Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00983
(730 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative / ... 64 7e-11
At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative / ... 64 7e-11
At5g44000.1 68418.m05384 glutathione S-transferase C-terminal do... 36 0.036
At2g18110.1 68415.m02105 elongation factor 1-beta, putative / EF... 34 0.11
At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta id... 32 0.34
At1g32190.1 68414.m03959 expressed protein 31 0.78
At5g15630.1 68418.m01829 phytochelatin synthetase family protein... 29 3.2
At3g28910.1 68416.m03608 myb family transcription factor (MYB30)... 29 4.2
At2g27090.1 68415.m03255 expressed protein contains Pfam domains... 29 4.2
At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase / lon... 29 4.2
At5g62470.2 68418.m07840 myb family transcription factor (MYB96)... 28 7.3
At5g26710.1 68418.m03168 glutamate-tRNA ligase, putative / gluta... 28 7.3
At5g07270.1 68418.m00829 ankyrin repeat family protein contains ... 28 7.3
At2g31180.1 68415.m03807 myb family transcription factor (MYB14)... 27 9.6
At1g77290.1 68414.m09001 tetrachloro-p-hydroquinone reductive de... 27 9.6
At1g27850.1 68414.m03413 expressed protein similar to En/Spm-lik... 27 9.6
>At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative similar to elongation factor 1B
gamma GI:3868758 from [Oryza sativa]
Length = 413
Score = 64.5 bits (150), Expect = 7e-11
Identities = 24/69 (34%), Positives = 42/69 (60%)
Frame = +3
Query: 510 FDPENYSIWYAEYKYPEELAKVFMSCNLITGMFQRLDKMRKQAFASVCLFGEDNNSTISG 689
+DPE YS+W+ +YKY +E F++ N + G QR+D RK +F + + G + + G
Sbjct: 293 YDPEGYSLWFCDYKYNDENMVSFVTLNKVGGFLQRMDLARKYSFGKMLICGSEGPFKVKG 352
Query: 690 VWVWRRKEL 716
+W++R E+
Sbjct: 353 LWLFRGPEI 361
Score = 53.6 bits (123), Expect = 1e-07
Identities = 29/87 (33%), Positives = 44/87 (50%)
Frame = +2
Query: 2 SDLLAALKVLDGHLLTRTFLVTERITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFL 181
S L L+ L+ HL + TFLV +TLAD++ L F V+ S+ +V+R+F
Sbjct: 132 SALKRGLEALNTHLASNTFLVGHSVTLADIVTICNLNLGFATVMTKKFTSAFPHVERYFW 191
Query: 182 TVAHQPQVSAVVGSLTLCAAPPTYALK 262
T+ +QP+ V+G A P K
Sbjct: 192 TMVNQPEFKKVLGDAKQTEAVPPVPTK 218
>At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative Similar to elongation factor
1-gamma (gb|EF1G_XENLA). ESTs
gb|T20564,gb|T45940,gb|T04527 come from this gene
Length = 414
Score = 64.5 bits (150), Expect = 7e-11
Identities = 24/69 (34%), Positives = 42/69 (60%)
Frame = +3
Query: 510 FDPENYSIWYAEYKYPEELAKVFMSCNLITGMFQRLDKMRKQAFASVCLFGEDNNSTISG 689
+DPE YS+W+ +YKY +E F++ N + G QR+D RK +F + + G + + G
Sbjct: 294 YDPEGYSLWFCDYKYNDENMVSFVTLNKVGGFLQRMDLARKYSFGKMLICGSEGPFKVKG 353
Query: 690 VWVWRRKEL 716
+W++R E+
Sbjct: 354 LWLFRGPEI 362
Score = 56.8 bits (131), Expect = 1e-08
Identities = 33/87 (37%), Positives = 45/87 (51%)
Frame = +2
Query: 2 SDLLAALKVLDGHLLTRTFLVTERITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFL 181
S L AL L+ HL + T+LV ITLAD+I L F V+ S +V+R+F
Sbjct: 132 STLKRALDALNTHLTSNTYLVGHSITLADIITVCNLNLGFATVMTKKFTSEFPHVERYFW 191
Query: 182 TVAHQPQVSAVVGSLTLCAAPPTYALK 262
TV +QP + V+G + A P A K
Sbjct: 192 TVVNQPNFTKVLGDVKQTEAVPPIASK 218
>At5g44000.1 68418.m05384 glutathione S-transferase C-terminal
domain-containing protein contains Pfam domain PF00043:
Glutathione S-transferase, C-terminal domain
Length = 399
Score = 35.5 bits (78), Expect = 0.036
Identities = 21/86 (24%), Positives = 42/86 (48%), Gaps = 2/86 (2%)
Frame = +2
Query: 2 SDLLAALKVLDGHLLTRTFLVTERITLADVIVFSTLLH--AFQHVLDPSVRSSLINVQRW 175
++L + L ++ HL + +L ER+TLADV +F+TL+ + ++L + L+
Sbjct: 270 NELFSTLDEIEDHLGSNRYLCGERLTLADVCLFTTLIRFDSVYNILFKCTKKKLVEYPNL 329
Query: 176 FLTVAHQPQVSAVVGSLTLCAAPPTY 253
+ + Q+ V + + A Y
Sbjct: 330 YGYLREIYQIPGVAATCDISAIMDGY 355
>At2g18110.1 68415.m02105 elongation factor 1-beta, putative /
EF-1-beta, putative nearly identical to eEF-1beta
[Arabidopsis thaliana] GI:398606
Length = 231
Score = 33.9 bits (74), Expect = 0.11
Identities = 21/67 (31%), Positives = 33/67 (49%)
Frame = +2
Query: 14 AALKVLDGHLLTRTFLVTERITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTVAH 193
+ LK LD HLLTR+++ + + D+ VF+ L S +NV RWF +
Sbjct: 11 SGLKKLDEHLLTRSYITGYQASKDDITVFT--------ALSKPPTSEFVNVSRWFNHIDA 62
Query: 194 QPQVSAV 214
++S V
Sbjct: 63 LLRISGV 69
>At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta
identical to SP|P48006 Elongation factor 1-beta
(EF-1-beta) {Arabidopsis thaliana}
Length = 231
Score = 32.3 bits (70), Expect = 0.34
Identities = 21/67 (31%), Positives = 34/67 (50%)
Frame = +2
Query: 14 AALKVLDGHLLTRTFLVTERITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTVAH 193
A LK LD HLLTR+++ + + D+ VF+ L P+ S +N RW+ +
Sbjct: 11 AGLKKLDEHLLTRSYITGYQASKDDITVFAAL------AKPPT--SQYVNASRWYNHIDA 62
Query: 194 QPQVSAV 214
++S V
Sbjct: 63 LLRISGV 69
>At1g32190.1 68414.m03959 expressed protein
Length = 422
Score = 31.1 bits (67), Expect = 0.78
Identities = 14/36 (38%), Positives = 16/36 (44%), Gaps = 1/36 (2%)
Frame = +1
Query: 625 CANRLSHPSACLVKTTIP-PSPECGCGAERSSCSRC 729
C + L PS K P PS CGCG C +C
Sbjct: 296 CCSGLCRPSCSCPKPRCPKPSCSCGCGCGDCGCFKC 331
>At5g15630.1 68418.m01829 phytochelatin synthetase family protein /
COBRA cell expansion protein COBL4 similar to
phytochelatin synthetase [Hordeum vulgare subsp.
vulgare] GI:29570314; identified in Roudier, et al,
Plant Phys. (2002) 130:538-548 (PMID:12376623); contains
Pfam profile PF04833: Phytochelatin synthetase-like
conserved region
Length = 431
Score = 29.1 bits (62), Expect = 3.2
Identities = 14/32 (43%), Positives = 16/32 (50%), Gaps = 6/32 (18%)
Frame = +1
Query: 643 HPSACL-----VKTTIPPSPECGCGAE-RSSC 720
HPS C+ TI P P C CG E + SC
Sbjct: 214 HPSCCVSFSSFYNDTITPCPSCACGCENKKSC 245
>At3g28910.1 68416.m03608 myb family transcription factor (MYB30)
identical to myb-like protein GB:AJ007289 [Arabidopsis
thaliana] (Plant J. 20 (1), 57-66 (1999))
Length = 323
Score = 28.7 bits (61), Expect = 4.2
Identities = 26/91 (28%), Positives = 39/91 (42%)
Frame = -1
Query: 415 RRGPWTP*AFQAPPLQGQEQLPPSSWVALSFHTSYLFCPPSCSVHQLTPGTF*GVCRRSR 236
++GPWTP QE P +W A+ +T L C SC + + T G+ R +
Sbjct: 13 KKGPWTPEEDIILVTYIQEH-GPGNWRAVPTNTGLLRCSKSCRL-RWTNYLRPGIKRGNF 70
Query: 235 TERERADDGRHLWLVGDGQEPTLNVYQRRTD 143
TE E L+G+ + +RTD
Sbjct: 71 TEHEEKMIVHLQALLGNRWAAIASYLPQRTD 101
>At2g27090.1 68415.m03255 expressed protein contains Pfam domains,
PF04782: Protein of unknown function (DUF632) and
PF04783: Protein of unknown function (DUF630)
Length = 743
Score = 28.7 bits (61), Expect = 4.2
Identities = 15/67 (22%), Positives = 33/67 (49%), Gaps = 1/67 (1%)
Frame = +3
Query: 513 DPENYSIWYAEYKYPEELAKVFMSCNLITGM-FQRLDKMRKQAFASVCLFGEDNNSTISG 689
+P S+ Y + + E ++ S N + GM ++++ F ++C+ + ST+
Sbjct: 374 EPAQNSVKYLTW-HRTESSRSSSSRNPLGGMNSDDVEELNSNLFENICMIAGSHASTLDR 432
Query: 690 VWVWRRK 710
++ W RK
Sbjct: 433 LYAWERK 439
>At1g49430.1 68414.m05541 long-chain-fatty-acid--CoA ligase /
long-chain acyl-CoA synthetase nearly identical to acyl
CoA synthetase (MF45P) GI:1617268 from [Brassica napus]
Length = 665
Score = 28.7 bits (61), Expect = 4.2
Identities = 13/44 (29%), Positives = 22/44 (50%)
Frame = -2
Query: 162 FISDERTLGSSTCWKACSSVLKTMTSASVILSVTRKVRVRRCPS 31
F+ ++ +C K CSS LKT+ S + S ++ +C S
Sbjct: 156 FVQEKTVSSILSCQKGCSSNLKTIVSFGEVSSTQKEEAKNQCVS 199
>At5g62470.2 68418.m07840 myb family transcription factor (MYB96)
contains Pfam profile: PF00249 myb-like DNA-binding
domain
Length = 352
Score = 27.9 bits (59), Expect = 7.3
Identities = 25/91 (27%), Positives = 37/91 (40%)
Frame = -1
Query: 415 RRGPWTP*AFQAPPLQGQEQLPPSSWVALSFHTSYLFCPPSCSVHQLTPGTF*GVCRRSR 236
++GPWTP QE P +W ++ HT C SC + + T G+ R +
Sbjct: 13 KKGPWTPEEDIILVSYIQEH-GPGNWRSVPTHTGLRRCSKSCRL-RWTNYLRPGIKRGNF 70
Query: 235 TERERADDGRHLWLVGDGQEPTLNVYQRRTD 143
TE E L+G+ + RTD
Sbjct: 71 TEHEEKTIVHLQALLGNRWAAIASYLPERTD 101
>At5g26710.1 68418.m03168 glutamate-tRNA ligase, putative /
glutamyl-tRNA synthetase, putatuve / GluRS, putative
identical to gi:3435196
Length = 719
Score = 27.9 bits (59), Expect = 7.3
Identities = 15/53 (28%), Positives = 27/53 (50%)
Frame = +2
Query: 29 LDGHLLTRTFLVTERITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTV 187
+D +L + TFLV +++ADV ++S L Q ++ RWF ++
Sbjct: 104 VDKYLESSTFLVGHSLSIADVAIWSALAGTGQRWESLRKSKKYQSLVRWFNSI 156
>At5g07270.1 68418.m00829 ankyrin repeat family protein contains
ankyrin repeats, Pfam:PF00023
Length = 513
Score = 27.9 bits (59), Expect = 7.3
Identities = 14/26 (53%), Positives = 14/26 (53%)
Frame = +3
Query: 42 FSHAPSLLPRESHLPMSLSSVHCCML 119
F PS L RE LPMSL CML
Sbjct: 368 FKRLPSSLTREMKLPMSLGFCAPCML 393
>At2g31180.1 68415.m03807 myb family transcription factor (MYB14)
similar to myb-related transcription factor GI:1370140
from [Lycopersicon esculentum]
Length = 249
Score = 27.5 bits (58), Expect = 9.6
Identities = 16/42 (38%), Positives = 19/42 (45%)
Frame = -1
Query: 415 RRGPWTP*AFQAPPLQGQEQLPPSSWVALSFHTSYLFCPPSC 290
+RGPWTP Q + S+W AL H L C SC
Sbjct: 13 KRGPWTPEEDQIL-INYIHLYGHSNWRALPKHAGLLRCGKSC 53
>At1g77290.1 68414.m09001 tetrachloro-p-hydroquinone reductive
dehalogenase-related contains similarity to
tetrachloro-p-hydroquinone reductive dehalogenase
GI:148689 from [Flavobacterium sp.]
Length = 266
Score = 27.5 bits (58), Expect = 9.6
Identities = 18/71 (25%), Positives = 31/71 (43%)
Frame = +2
Query: 8 LLAALKVLDGHLLTRTFLVTERITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTV 187
LL L ++ L T+L ++ADV++ L L+ SS N+ ++ V
Sbjct: 168 LLRLLDEVETKLEGTTYLAGNEFSMADVMLIPVLARLSLLDLEEEYISSRKNLAEYWALV 227
Query: 188 AHQPQVSAVVG 220
+P V+G
Sbjct: 228 RRRPSYKKVIG 238
>At1g27850.1 68414.m03413 expressed protein similar to En/Spm-like
transposon protein GB:AAB95292 GI:2088658 from
[Arabidopsis thaliana]
Length = 1148
Score = 27.5 bits (58), Expect = 9.6
Identities = 14/28 (50%), Positives = 18/28 (64%)
Frame = -3
Query: 224 ASRRRPTLVAGGRRSGTNAERLSATNGR 141
+S R P+ A GRRSGT R+S T G+
Sbjct: 171 SSARHPS-PASGRRSGTPVRRISPTPGK 197
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,763,931
Number of Sequences: 28952
Number of extensions: 329104
Number of successful extensions: 928
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 896
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 926
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1594686376
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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