BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00979 (736 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g21280.1 68416.m02689 ubiquitin-specific protease 7, putative... 67 1e-11 At1g51710.1 68414.m05827 ubiquitin-specific protease 6, putative... 64 9e-11 At5g57990.1 68418.m07255 ubiquitin-specific protease 23, putativ... 33 0.15 At5g45060.1 68418.m05525 disease resistance protein (TIR-NBS-LRR... 32 0.45 At2g34357.1 68415.m04206 expressed protein 32 0.45 At5g45250.1 68418.m05553 disease resistance protein (TIR-NBS-LRR... 31 0.60 At3g14400.1 68416.m01822 ubiquitin-specific protease 25 (UBP25) ... 30 1.4 At3g20630.1 68416.m02610 ubiquitin-specific protease 14, putativ... 29 3.2 At2g32905.1 68415.m04034 hypothetical protein contains Pfam prof... 29 3.2 At1g76140.1 68414.m08842 prolyl oligopeptidase, putative / proly... 29 4.2 At5g10790.1 68418.m01254 ubiquitin-specific protease 22 (UBP22) ... 28 7.4 At3g60860.1 68416.m06808 guanine nucleotide exchange family prot... 28 7.4 At3g52890.2 68416.m05829 protein kinase (KIPK) identical to prot... 28 7.4 At3g52890.1 68416.m05828 protein kinase (KIPK) identical to prot... 28 7.4 At5g17930.1 68418.m02102 MA3 domain-containing protein low simil... 27 9.8 >At3g21280.1 68416.m02689 ubiquitin-specific protease 7, putative (UBP7) similar to GI:11993467 Length = 532 Score = 66.9 bits (156), Expect = 1e-11 Identities = 28/72 (38%), Positives = 49/72 (68%) Frame = +2 Query: 254 EFVRFYYKEKESINAKILKDVKFPLDLDVYELCTPELQERLTPMRNKFKELEDAKVQSSL 433 +FVRF++K + + AKIL+ V +PL+LD+Y+LC+ +L+++L R K +++E K+ Sbjct: 361 QFVRFFWKRESNQKAKILRKVDYPLELDIYDLCSEDLRKKLEAPRQKLRDIEGQKLGLQA 420 Query: 434 SSGNKSHGDSSK 469 S+ + S GD K Sbjct: 421 SAKSSSKGDDVK 432 Score = 66.9 bits (156), Expect = 1e-11 Identities = 28/63 (44%), Positives = 41/63 (65%), Gaps = 1/63 (1%) Frame = +3 Query: 549 VLTHRGRSSSSGHYVAWVARGAG-WLRCXXXXXXXXXXXXXLKLSGGSDWHCAYLLLYGP 725 VLTH+GRS+ SGHYVAWV + +G W++ +KLSGG DWH AY+++Y Sbjct: 468 VLTHKGRSADSGHYVAWVKQESGKWVQYDDANTSLQRGEDIIKLSGGGDWHMAYIVMYKA 527 Query: 726 KIL 734 +++ Sbjct: 528 RLI 530 Score = 61.7 bits (143), Expect = 5e-10 Identities = 34/83 (40%), Positives = 45/83 (54%) Frame = +3 Query: 6 IEQYFGGTLDVELVCSEADEPPTHSEESFLQLSCFISQDVKYLQSGLRSKMAEHITKMSQ 185 ++ FG L L C E+ E + +E F L C IS +V +L GL+ + + K S Sbjct: 279 VKALFGLNLLNRLHCQESSEESSETESVF-SLKCHISHEVNHLHEGLKHGLKGELEKTSP 337 Query: 186 TLGRDAVYTKTSKISRLPAYLTV 254 +LGR AVY K S I LP YLTV Sbjct: 338 SLGRTAVYVKESLIDSLPRYLTV 360 >At1g51710.1 68414.m05827 ubiquitin-specific protease 6, putative (UBP6) similar to GI:11993465 Length = 482 Score = 64.1 bits (149), Expect = 9e-11 Identities = 28/58 (48%), Positives = 36/58 (62%), Gaps = 1/58 (1%) Frame = +3 Query: 549 VLTHRGRSSSSGHYVAWVARGAG-WLRCXXXXXXXXXXXXXLKLSGGSDWHCAYLLLY 719 VLTH+GRS+ SGHYVAWV + +G W++ KLSGG DWH AY+ +Y Sbjct: 418 VLTHKGRSADSGHYVAWVKQESGKWIQYDDDNPSMQREEDITKLSGGGDWHMAYITMY 475 Score = 61.3 bits (142), Expect = 6e-10 Identities = 33/83 (39%), Positives = 44/83 (53%) Frame = +3 Query: 6 IEQYFGGTLDVELVCSEADEPPTHSEESFLQLSCFISQDVKYLQSGLRSKMAEHITKMSQ 185 ++ FG L L C E+ E + +E S L C IS +V +L GL+ + + K S Sbjct: 225 VKALFGVNLQSRLHCQESGEESSETE-SVYSLKCHISHEVNHLHEGLKHGLKGELEKTSP 283 Query: 186 TLGRDAVYTKTSKISRLPAYLTV 254 LGR A+Y K S I LP YLTV Sbjct: 284 ALGRTALYVKESLIDSLPRYLTV 306 Score = 60.5 bits (140), Expect = 1e-09 Identities = 28/67 (41%), Positives = 47/67 (70%), Gaps = 2/67 (2%) Frame = +2 Query: 254 EFVRFYYKEKESINAKILKDVKFPLDLDVYELCTPELQERLTPMRNKFKELEDAK--VQS 427 +FVRF++K + + AKIL+ V +PL LD+++LC+ +L+++L R K +E E K +Q+ Sbjct: 307 QFVRFFWKRESNQKAKILRKVDYPLVLDIFDLCSEDLRKKLEAPRQKLREEEGKKLGLQT 366 Query: 428 SLSSGNK 448 S SG+K Sbjct: 367 SAKSGSK 373 >At5g57990.1 68418.m07255 ubiquitin-specific protease 23, putative (UBP23) identical to GI:11993486 Length = 859 Score = 33.5 bits (73), Expect = 0.15 Identities = 14/24 (58%), Positives = 16/24 (66%) Frame = +3 Query: 546 GVLTHRGRSSSSGHYVAWVARGAG 617 GVL H GRSS SGHY +V +G Sbjct: 356 GVLVHYGRSSHSGHYACFVRTSSG 379 >At5g45060.1 68418.m05525 disease resistance protein (TIR-NBS-LRR class), putative domain signature TIR-NBS-LRR exists, suggestive of a disease resistance protein. Length = 1165 Score = 31.9 bits (69), Expect = 0.45 Identities = 16/38 (42%), Positives = 24/38 (63%) Frame = +3 Query: 39 ELVCSEADEPPTHSEESFLQLSCFISQDVKYLQSGLRS 152 E+V DE +++FL ++CF SQDV Y++S L S Sbjct: 448 EVVQVSYDELSLAQKDAFLDIACFRSQDVDYVESLLVS 485 >At2g34357.1 68415.m04206 expressed protein Length = 1280 Score = 31.9 bits (69), Expect = 0.45 Identities = 15/34 (44%), Positives = 22/34 (64%) Frame = +2 Query: 386 RNKFKELEDAKVQSSLSSGNKSHGDSSKAKKKRV 487 R+K KEL DA + S G++ G+SSK +KR+ Sbjct: 1149 RSKRKELSDADSDAKSSKGSRFSGNSSKKNQKRM 1182 >At5g45250.1 68418.m05553 disease resistance protein (TIR-NBS-LRR class), putative (RPS4) domain signature TIR-NBS-LRR exists, suggestive of a disease resistance protein. Identical to RPS4 (GI:11357255). False intron created at intron 2 to escape a frameshift in the BAC sequence. Length = 1217 Score = 31.5 bits (68), Expect = 0.60 Identities = 14/31 (45%), Positives = 21/31 (67%) Frame = +3 Query: 60 DEPPTHSEESFLQLSCFISQDVKYLQSGLRS 152 DE T +++FL ++CF SQD Y++S L S Sbjct: 447 DELTTAQKDAFLDIACFRSQDKDYVESLLAS 477 >At3g14400.1 68416.m01822 ubiquitin-specific protease 25 (UBP25) similar to GI:11993490 Length = 661 Score = 30.3 bits (65), Expect = 1.4 Identities = 13/29 (44%), Positives = 16/29 (55%), Gaps = 1/29 (3%) Frame = +3 Query: 546 GVLTHRGRSSSSGHYVAWVARGAG-WLRC 629 G++ H G S SGHY A+V G W C Sbjct: 281 GIIVHSGFSPESGHYYAYVKDSLGRWYCC 309 >At3g20630.1 68416.m02610 ubiquitin-specific protease 14, putative (UBP14) similar to ubiquitin-specific protease 14 GI:11993473 [Arabidopsis thaliana] Length = 797 Score = 29.1 bits (62), Expect = 3.2 Identities = 10/26 (38%), Positives = 16/26 (61%) Frame = +3 Query: 546 GVLTHRGRSSSSGHYVAWVARGAGWL 623 G+++H G S GHYVA + + W+ Sbjct: 745 GIVSHMGTSVHCGHYVAHILKEGRWV 770 >At2g32905.1 68415.m04034 hypothetical protein contains Pfam profile PF03754: Domain of unknown function (DUF313) Length = 205 Score = 29.1 bits (62), Expect = 3.2 Identities = 19/57 (33%), Positives = 26/57 (45%), Gaps = 3/57 (5%) Frame = +1 Query: 7 SSNTSEVPWMWSLYAQR---LMSPRPTLRSHSSSCPVSYHKTSNICSLD*DRKWLSI 168 SSN + PW + QR L +P P SSSC + K ++ + RK L I Sbjct: 71 SSNKTITPWTRVIEQQRSSELQNPNPESEPSSSSCVTHFKKRRHMDKEESTRKRLKI 127 >At1g76140.1 68414.m08842 prolyl oligopeptidase, putative / prolyl endopeptidase, putative / post-proline cleaving enzyme, putative similar to SP|Q9QUR6 Prolyl endopeptidase (EC 3.4.21.26) (Post-proline cleaving enzyme) {Mus musculus}; contains Pfam profiles PF00326: prolyl oligopeptidase family, PF02897: Prolyl oligopeptidase, N-terminal beta-propeller domain Length = 731 Score = 28.7 bits (61), Expect = 4.2 Identities = 14/38 (36%), Positives = 24/38 (63%), Gaps = 1/38 (2%) Frame = +3 Query: 63 EPPTHSEESFLQLSCF-ISQDVKYLQSGLRSKMAEHIT 173 +P T S++ + L+ F +S+D KYL GL S ++ +T Sbjct: 122 DPNTLSDDGTVALNTFSVSEDAKYLAYGLSSSGSDWVT 159 >At5g10790.1 68418.m01254 ubiquitin-specific protease 22 (UBP22) almost identical to ubiquitin-specific protease 22 GI:11993484 [Arabidopsis thaliana], one amino acid difference Length = 557 Score = 27.9 bits (59), Expect = 7.4 Identities = 12/27 (44%), Positives = 16/27 (59%) Frame = +3 Query: 549 VLTHRGRSSSSGHYVAWVARGAGWLRC 629 V+TH+G S GHYV ++ W RC Sbjct: 480 VVTHKGMLES-GHYVTYLRLKGLWYRC 505 >At3g60860.1 68416.m06808 guanine nucleotide exchange family protein similar to guanine nucleotide exchange factor, Homo sapiens, GI:5456754; contains Pfam profile PF01369: Sec7 domain Length = 1793 Score = 27.9 bits (59), Expect = 7.4 Identities = 19/72 (26%), Positives = 29/72 (40%), Gaps = 2/72 (2%) Frame = +2 Query: 293 NAKILKDVKFPLDLDVYELCTPELQER--LTPMRNKFKELEDAKVQSSLSSGNKSHGDSS 466 N + KD+ + C +L E +P NK+K QSSL SG ++ Sbjct: 1278 NNRFSKDISLS-SIAFLRYCATKLAEGDLNSPSTNKYKGTSGKIPQSSLHSGKSGKQENG 1336 Query: 467 KAKKKRVLPYWF 502 + L +WF Sbjct: 1337 EIVNNNHLYFWF 1348 >At3g52890.2 68416.m05829 protein kinase (KIPK) identical to protein kinase KIPK (KCBP-interacting protein kinase) [Arabidopsis thaliana] gi|7716430|gb|AAF68383 Length = 934 Score = 27.9 bits (59), Expect = 7.4 Identities = 19/63 (30%), Positives = 27/63 (42%) Frame = +3 Query: 84 ESFLQLSCFISQDVKYLQSGLRSKMAEHITKMSQTLGRDAVYTKTSKISRLPAYLTVNSF 263 E Q+SCF + Q G + K +H++K Q L R ++ P NSF Sbjct: 725 EPSCQVSCFSPRLSSNQQQGRKPKRGDHLSKTQQHLSR-----SLPQLVAEPTEARSNSF 779 Query: 264 VST 272 V T Sbjct: 780 VGT 782 >At3g52890.1 68416.m05828 protein kinase (KIPK) identical to protein kinase KIPK (KCBP-interacting protein kinase) [Arabidopsis thaliana] gi|7716430|gb|AAF68383 Length = 934 Score = 27.9 bits (59), Expect = 7.4 Identities = 19/63 (30%), Positives = 27/63 (42%) Frame = +3 Query: 84 ESFLQLSCFISQDVKYLQSGLRSKMAEHITKMSQTLGRDAVYTKTSKISRLPAYLTVNSF 263 E Q+SCF + Q G + K +H++K Q L R ++ P NSF Sbjct: 725 EPSCQVSCFSPRLSSNQQQGRKPKRGDHLSKTQQHLSR-----SLPQLVAEPTEARSNSF 779 Query: 264 VST 272 V T Sbjct: 780 VGT 782 >At5g17930.1 68418.m02102 MA3 domain-containing protein low similarity to SP|Q9P6R9 Cell cycle control protein cwf22 {Schizosaccharomyces pombe}; contains Pfam profile PF02847: MA3 domain Length = 707 Score = 27.5 bits (58), Expect = 9.8 Identities = 14/34 (41%), Positives = 21/34 (61%), Gaps = 3/34 (8%) Frame = +2 Query: 395 FKELEDA---KVQSSLSSGNKSHGDSSKAKKKRV 487 F+++ D+ ++SS SGNK HG S KK+ V Sbjct: 34 FRQISDSVTEHMESSSPSGNKDHGKSFIPKKEEV 67 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,197,479 Number of Sequences: 28952 Number of extensions: 279053 Number of successful extensions: 797 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 771 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 795 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1614253080 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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