BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00974 (420 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1002.03c |gls2||glucosidase II Gls2|Schizosaccharomyces pomb... 79 4e-16 SPAC30D11.01c ||SPAC56F8.01|alpha-glucosidase|Schizosaccharomyce... 46 3e-06 SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces... 42 3e-05 SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyc... 39 3e-04 SPAC6F6.06c |rax2||cell polarity factor Rax2|Schizosaccharomyces... 29 0.29 SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S... 26 2.7 SPAC1805.13 |rpl14||60S ribosomal protein L14|Schizosaccharomyce... 25 3.6 SPBC25H2.02 |ths1||threonine-tRNA ligase Ths1 |Schizosaccharomyc... 25 4.8 SPAC22F3.13 |tsc1||hamartin|Schizosaccharomyces pombe|chr 1|||Ma... 25 6.3 SPBC839.02 |||arrestin Aly1 related|Schizosaccharomyces pombe|ch... 25 6.3 >SPAC1002.03c |gls2||glucosidase II Gls2|Schizosaccharomyces pombe|chr 1|||Manual Length = 923 Score = 78.6 bits (185), Expect = 4e-16 Identities = 37/84 (44%), Positives = 49/84 (58%) Frame = +2 Query: 2 PGSSSYPDFFNPVVSRYYSDRYRFENFPGTSKDVHLWNDMNEPSVFNGPEVTMPKDCRHY 181 PG+S + DFFNP ++ Y F+ F + K++ +WNDMNEPSVF GPE +M +D HY Sbjct: 487 PGNSVWVDFFNPEAQAWWGSLYEFDRFE-SDKNLWIWNDMNEPSVFRGPETSMHRDAIHY 545 Query: 182 KPPQDGLEGLADYWEHRHVHNEYG 253 WEHR +HN YG Sbjct: 546 -----------GGWEHRDIHNIYG 558 Score = 42.3 bits (95), Expect = 3e-05 Identities = 17/36 (47%), Positives = 21/36 (58%) Frame = +3 Query: 309 RPFLLTRAVXAGTQRYSAVWTGDNTAEWSFLAASVP 416 RPF+LTR+ AGT +A W GD W L S+P Sbjct: 578 RPFILTRSFFAGTSALAANWIGDTMTTWEHLRGSIP 613 >SPAC30D11.01c ||SPAC56F8.01|alpha-glucosidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 993 Score = 45.6 bits (103), Expect = 3e-06 Identities = 17/35 (48%), Positives = 26/35 (74%) Frame = +3 Query: 309 RPFLLTRAVXAGTQRYSAVWTGDNTAEWSFLAASV 413 RPFLL+R+ G+ RY+A W GDN ++WS + +S+ Sbjct: 650 RPFLLSRSTFVGSGRYAAHWLGDNKSQWSDMVSSI 684 Score = 26.6 bits (56), Expect = 1.6 Identities = 10/18 (55%), Positives = 12/18 (66%) Frame = +2 Query: 2 PGSSSYPDFFNPVVSRYY 55 PG YPDF NP V +Y+ Sbjct: 457 PGFVVYPDFTNPAVLQYW 474 >SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 995 Score = 42.3 bits (95), Expect = 3e-05 Identities = 16/36 (44%), Positives = 25/36 (69%) Frame = +3 Query: 309 RPFLLTRAVXAGTQRYSAVWTGDNTAEWSFLAASVP 416 RPF+L+R+ G+ +Y+A W GDN + WS + S+P Sbjct: 647 RPFILSRSTFVGSGKYAAHWLGDNYSLWSNMIFSIP 682 Score = 30.3 bits (65), Expect = 0.13 Identities = 18/54 (33%), Positives = 29/54 (53%), Gaps = 8/54 (14%) Frame = +2 Query: 2 PGSSSYPDFFNPVVSRYYSD-------RYRFEN-FPGTSKDVHLWNDMNEPSVF 139 PG +++PDF NP + Y+++ + + + FP LW DMNEP+ F Sbjct: 455 PGFTTFPDFTNPETTNYWTECLMNLSAAFGYNSSFPLPYSG--LWIDMNEPTSF 506 >SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyces pombe|chr 1|||Manual Length = 969 Score = 39.1 bits (87), Expect = 3e-04 Identities = 22/51 (43%), Positives = 28/51 (54%), Gaps = 5/51 (9%) Frame = +2 Query: 2 PGSSSYPDFFNPVVSRYYSD-----RYRFENFPGTSKDVHLWNDMNEPSVF 139 PG +++PDF NP V Y+ D Y F + GT +W DMNEPS F Sbjct: 439 PGFTAFPDFTNPDVVDYWKDCLINLTYAFGS-NGTVPFSGIWTDMNEPSSF 488 Score = 36.3 bits (80), Expect = 0.002 Identities = 15/35 (42%), Positives = 22/35 (62%) Frame = +3 Query: 309 RPFLLTRAVXAGTQRYSAVWTGDNTAEWSFLAASV 413 RPF+L+R+ G+ Y A W GDN + WS + S+ Sbjct: 625 RPFILSRSTFLGSGVYGAHWLGDNHSLWSNMFFSI 659 >SPAC6F6.06c |rax2||cell polarity factor Rax2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1155 Score = 29.1 bits (62), Expect = 0.29 Identities = 17/47 (36%), Positives = 27/47 (57%), Gaps = 3/47 (6%) Frame = -1 Query: 168 SLGMVTSGPLKTLGS---FISFHRWTSLLVPGKFSNLYRSE*YRLTT 37 SLG+ T+GP+ +GS FI + + G F+ +Y SE Y ++T Sbjct: 633 SLGLGTNGPVTHIGSTSLFIDGKIESFISFQGDFNEVYTSEGYAIST 679 >SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|Schizosaccharomyces pombe|chr 1|||Manual Length = 2397 Score = 25.8 bits (54), Expect = 2.7 Identities = 12/51 (23%), Positives = 25/51 (49%) Frame = +2 Query: 35 PVVSRYYSDRYRFENFPGTSKDVHLWNDMNEPSVFNGPEVTMPKDCRHYKP 187 P+VS + ++ + N + +H+++D ++P V E P + Y P Sbjct: 1757 PIVSNHQTEGSQMFNEISSVSSIHVYHDESQPPVEMPAESDTPLQNKLYHP 1807 >SPAC1805.13 |rpl14||60S ribosomal protein L14|Schizosaccharomyces pombe|chr 1|||Manual Length = 134 Score = 25.4 bits (53), Expect = 3.6 Identities = 8/17 (47%), Positives = 11/17 (64%) Frame = +1 Query: 331 PCXQAPRDTLRYGQVTI 381 PC + PR +RYG V + Sbjct: 42 PCSEFPRQVIRYGSVVL 58 >SPBC25H2.02 |ths1||threonine-tRNA ligase Ths1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 703 Score = 25.0 bits (52), Expect = 4.8 Identities = 10/38 (26%), Positives = 18/38 (47%) Frame = +3 Query: 285 AGPRRRVYRPFLLTRAVXAGTQRYSAVWTGDNTAEWSF 398 +G + RP ++ RA+ +R A+ T +W F Sbjct: 556 SGNNNKYTRPVMVHRAILGSLERMIAILTEHYAGKWPF 593 >SPAC22F3.13 |tsc1||hamartin|Schizosaccharomyces pombe|chr 1|||Manual Length = 899 Score = 24.6 bits (51), Expect = 6.3 Identities = 13/46 (28%), Positives = 21/46 (45%), Gaps = 1/46 (2%) Frame = +2 Query: 8 SSSYPDFFNPVVS-RYYSDRYRFENFPGTSKDVHLWNDMNEPSVFN 142 S YPD + Y RY +N + K H+ +++ E + FN Sbjct: 19 SEGYPDLTELIAEVESYQQRYPKQNPTNSQKIRHILDEIYEKTPFN 64 >SPBC839.02 |||arrestin Aly1 related|Schizosaccharomyces pombe|chr 2|||Manual Length = 530 Score = 24.6 bits (51), Expect = 6.3 Identities = 15/39 (38%), Positives = 20/39 (51%) Frame = +3 Query: 96 RTSTCGTI*TNPVSSTVQRSPCPRTVATTSHPRTVWRGW 212 R+ST T + + RSPC +VA TS P V + W Sbjct: 226 RSSTFSGKSTGRIPIELIRSPCSTSVA-TSEPILVSKSW 263 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,698,397 Number of Sequences: 5004 Number of extensions: 33964 Number of successful extensions: 122 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 109 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 120 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 148351622 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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