Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00974
(420 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g63840.1 68418.m08014 alpha-glucosidase, putative similar to ... 88 3e-18
At3g23640.1 68416.m02973 glycosyl hydrolase family 31 protein si... 51 3e-07
At5g11720.1 68418.m01369 alpha-glucosidase 1 (AGLU1) identical t... 47 5e-06
At3g45940.1 68416.m04971 alpha-xylosidase, putative strong simil... 43 1e-04
At1g68560.1 68414.m07833 alpha-xylosidase (XYL1) identical to al... 43 1e-04
At1g20110.1 68414.m02516 zinc finger (FYVE type) family protein ... 28 3.0
At5g24280.1 68418.m02856 expressed protein ; expression supporte... 27 3.9
At5g22240.1 68418.m02589 ovate family protein 60% similar to ova... 26 9.0
At5g01130.1 68418.m00017 hypothetical protein contains Pfam prof... 26 9.0
At2g40470.1 68415.m04994 LOB domain protein 15 / lateral organ b... 26 9.0
>At5g63840.1 68418.m08014 alpha-glucosidase, putative similar to
alpha-glucosidase GI:2648032 from [Solanum tuberosum]
Length = 921
Score = 87.8 bits (208), Expect = 3e-18
Identities = 40/84 (47%), Positives = 56/84 (66%)
Frame = +2
Query: 2 PGSSSYPDFFNPVVSRYYSDRYRFENFPGTSKDVHLWNDMNEPSVFNGPEVTMPKDCRHY 181
PGSSSY D +P + +++ R+ ++N+ G++ ++ WNDMNEPSVFNGPEVTMP+D H
Sbjct: 474 PGSSSYIDMLSPEIRKWWGGRFSYKNYVGSTPSLYTWNDMNEPSVFNGPEVTMPRDALH- 532
Query: 182 KPPQDGLEGLADYWEHRHVHNEYG 253
+ G+ EHR VHN YG
Sbjct: 533 ------VGGV----EHREVHNAYG 546
Score = 64.1 bits (149), Expect = 4e-11
Identities = 25/37 (67%), Positives = 30/37 (81%)
Frame = +3
Query: 309 RPFLLTRAVXAGTQRYSAVWTGDNTAEWSFLAASVPM 419
RPF+L+RA+ GTQRY A+WTGDNTAEW L S+PM
Sbjct: 566 RPFVLSRAIFPGTQRYGAIWTGDNTAEWEHLRVSIPM 602
>At3g23640.1 68416.m02973 glycosyl hydrolase family 31 protein
similar to alpha-glucosidase II from SP:Q9F234 [Bacillus
thermoamyloliquefaciens]; contains Pfam profile: PF01055
Glycosyl hydrolases family 31
Length = 991
Score = 51.2 bits (117), Expect = 3e-07
Identities = 21/37 (56%), Positives = 26/37 (70%)
Frame = +3
Query: 309 RPFLLTRAVXAGTQRYSAVWTGDNTAEWSFLAASVPM 419
RPF+LTRA G+QRY+A WTGDN + W L S+ M
Sbjct: 404 RPFVLTRAGFIGSQRYAATWTGDNLSNWEHLHMSISM 440
Score = 48.4 bits (110), Expect = 2e-06
Identities = 28/84 (33%), Positives = 41/84 (48%)
Frame = +2
Query: 2 PGSSSYPDFFNPVVSRYYSDRYRFENFPGTSKDVHLWNDMNEPSVFNGPEVTMPKDCRHY 181
PG +PD+ N ++++ + F D +WNDMNEP+VF TMP++ H+
Sbjct: 310 PGPCVFPDYTNSKARSWWANLVK--EFVSNGVD-GIWNDMNEPAVFKVVTKTMPENNIHH 366
Query: 182 KPPQDGLEGLADYWEHRHVHNEYG 253
G + L H H HN YG
Sbjct: 367 -----GDDELGGVQNHSHYHNVYG 385
>At5g11720.1 68418.m01369 alpha-glucosidase 1 (AGLU1) identical to
alpha-glucosidase 1 [Arabidopsis thaliana] GI:2323344
Length = 902
Score = 47.2 bits (107), Expect = 5e-06
Identities = 18/36 (50%), Positives = 26/36 (72%)
Frame = +3
Query: 309 RPFLLTRAVXAGTQRYSAVWTGDNTAEWSFLAASVP 416
RPF+L+R+ + +Y+A WTGDN A+W LA S+P
Sbjct: 536 RPFILSRSTFVSSGKYTAHWTGDNAAKWEDLAYSIP 571
Score = 32.3 bits (70), Expect = 0.14
Identities = 17/46 (36%), Positives = 23/46 (50%)
Frame = +2
Query: 2 PGSSSYPDFFNPVVSRYYSDRYRFENFPGTSKDVHLWNDMNEPSVF 139
PG +PDF NP + ++S+ + F LW DMNE S F
Sbjct: 423 PGKVYFPDFLNPAAATFWSNEIKM--FQEILPLDGLWIDMNELSNF 466
>At3g45940.1 68416.m04971 alpha-xylosidase, putative strong
similarity to alpha-xylosidase precursor GI:4163997 from
[Arabidopsis thaliana]
Length = 868
Score = 42.7 bits (96), Expect = 1e-04
Identities = 16/35 (45%), Positives = 23/35 (65%)
Frame = +3
Query: 309 RPFLLTRAVXAGTQRYSAVWTGDNTAEWSFLAASV 413
RPF+L+R+ G+ +Y+A WTGDN W L S+
Sbjct: 493 RPFILSRSTFVGSGQYAAHWTGDNQGTWQSLQVSI 527
Score = 29.5 bits (63), Expect = 0.97
Identities = 16/42 (38%), Positives = 19/42 (45%)
Frame = +2
Query: 2 PGSSSYPDFFNPVVSRYYSDRYRFENFPGTSKDVHLWNDMNE 127
PG +PDF NP ++ D R F LW DMNE
Sbjct: 401 PGPVYFPDFLNPKTVSWWGDEIR--RFHELVPIDGLWIDMNE 440
>At1g68560.1 68414.m07833 alpha-xylosidase (XYL1) identical to
alpha-xylosidase precursor GB:AAD05539 GI:4163997 from
[Arabidopsis thaliana]; contains Pfam profile PF01055:
Glycosyl hydrolases family 31; identical to cDNA
alpha-xylosidase precursor (XYL1) partial cds GI:4163996
Length = 915
Score = 42.7 bits (96), Expect = 1e-04
Identities = 16/35 (45%), Positives = 23/35 (65%)
Frame = +3
Query: 309 RPFLLTRAVXAGTQRYSAVWTGDNTAEWSFLAASV 413
RPF+L+R+ G+ +Y+A WTGDN W L S+
Sbjct: 541 RPFILSRSTFVGSGQYAAHWTGDNQGTWQSLQVSI 575
Score = 32.7 bits (71), Expect = 0.10
Identities = 19/55 (34%), Positives = 25/55 (45%)
Frame = +2
Query: 2 PGSSSYPDFFNPVVSRYYSDRYRFENFPGTSKDVHLWNDMNEPSVFNGPEVTMPK 166
PG +PDF NP ++ D + F LW DMNE S F T+P+
Sbjct: 404 PGPVYFPDFLNPKTVSWWGD--EIKRFHDLVPIDGLWIDMNEVSNFCSGLCTIPE 456
>At1g20110.1 68414.m02516 zinc finger (FYVE type) family protein
contains Pfam profile: PF01363 FYVE zinc finger
Length = 601
Score = 27.9 bits (59), Expect = 3.0
Identities = 22/86 (25%), Positives = 40/86 (46%), Gaps = 2/86 (2%)
Frame = +2
Query: 50 YYSDRY-RFENFPGTSKDVHLWNDMNEPSVFNGPEVTMPKDCRHYKPPQDGLEGLADYWE 226
YY +Y +F+N P + + + P+ GP + ++ PP G G ADY
Sbjct: 9 YYHHQYSQFQN-PTPNPNPNPNPSPPAPATVAGP-TDLTRNTYASAPPFTGGYGSADYSN 66
Query: 227 HRHVHNEYGRGT-CAPPTRGCWTAPT 301
+ + YG+ + PP+ +T+P+
Sbjct: 67 YSQNYTPYGQNSEHVPPSAPSFTSPS 92
>At5g24280.1 68418.m02856 expressed protein ; expression supported by
MPSS
Length = 1634
Score = 27.5 bits (58), Expect = 3.9
Identities = 14/27 (51%), Positives = 16/27 (59%)
Frame = +1
Query: 109 VERYERTQCLQRSRGHHAQGLSPLQAT 189
VE ER +CLQ R H Q S LQA+
Sbjct: 1309 VELEERLKCLQAQREHAEQECSRLQAS 1335
>At5g22240.1 68418.m02589 ovate family protein 60% similar to ovate
protein (GI:23429649) [Lycopersicon esculentum];
contains TIGRFAM TIGR01568 : uncharacterized
plant-specific domain TIGR01568
Length = 196
Score = 26.2 bits (55), Expect = 9.0
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = +3
Query: 129 PVSSTVQRSPCPR 167
PVS TV RSPCP+
Sbjct: 81 PVSPTVLRSPCPK 93
>At5g01130.1 68418.m00017 hypothetical protein contains Pfam profile
PF05056: Protein of unknown function (DUF674)
Length = 484
Score = 26.2 bits (55), Expect = 9.0
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = -1
Query: 144 PLKTLGSFISFHRWTSLLVPGKFSNLYRS 58
P+ T+ + HR + + G FSNLYRS
Sbjct: 43 PMGTIIRLLEEHRESQPITVGCFSNLYRS 71
>At2g40470.1 68415.m04994 LOB domain protein 15 / lateral organ
boundaries domain protein 15 (LBD15) identical to
SP|Q8L5T5 LOB domain protein 15 {Arabidopsis thaliana};
similar to ASYMMETRIC LEAVES2 [Arabidopsis thaliana]
GI:19918971
Length = 224
Score = 26.2 bits (55), Expect = 9.0
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = -1
Query: 414 ARKPQGTTTPPYCHLSIPQSISGCLXTRPS 325
A PQ TTPP + P S S C+ ++P+
Sbjct: 178 APPPQRPTTPPQPTTAHPPSPSSCVFSQPT 207
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,450,480
Number of Sequences: 28952
Number of extensions: 199912
Number of successful extensions: 581
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 558
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 577
length of database: 12,070,560
effective HSP length: 74
effective length of database: 9,928,112
effective search space used: 645327280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -