BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00964 (427 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g29210.1 68415.m03550 splicing factor PWI domain-containing p... 34 0.035 At1g16610.2 68414.m01990 arginine/serine-rich protein, putative ... 33 0.081 At1g16610.1 68414.m01989 arginine/serine-rich protein, putative ... 33 0.081 At1g78230.1 68414.m09116 leucine-rich repeat family protein 29 1.00 At3g63030.1 68416.m07080 methyl-CpG-binding domain-containing pr... 28 2.3 At2g47350.1 68415.m05911 PAPA-1-like family protein / zinc finge... 28 2.3 At4g12610.1 68417.m01987 transcription initiation factor IIF alp... 28 3.0 At5g63920.1 68418.m08026 DNA topoisomerase III alpha, putative s... 27 4.0 At1g69485.1 68414.m07984 hypothetical protein 27 5.3 At5g54260.1 68418.m06759 DNA repair and meiosis protein (Mre11) ... 26 9.3 At5g53500.1 68418.m06649 WD-40 repeat family protein contains Pf... 26 9.3 At3g07220.1 68416.m00861 transcriptional activator, putative sim... 26 9.3 At1g44850.1 68414.m05138 hypothetical protein 26 9.3 At1g14610.1 68414.m01737 valyl-tRNA synthetase / valine--tRNA li... 26 9.3 >At2g29210.1 68415.m03550 splicing factor PWI domain-containing protein contains Pfam profile PF01480: PWI domain Length = 878 Score = 34.3 bits (75), Expect = 0.035 Identities = 25/75 (33%), Positives = 32/75 (42%), Gaps = 5/75 (6%) Frame = +1 Query: 88 RQENYGGERRSFGGDERRRSPARESYRKPSGXXPPRHPRIGTRPLRPFR-----GRTLRS 252 R+ + G R+S RRRSP+ + R+ S P R R + P R R R RS Sbjct: 293 RRPTHEGRRQSPAPSRRRRSPSPPARRRRSPSPPARRRRSPSPPARRHRSPTPPARQRRS 352 Query: 253 RHPIAAHRARVPPLR 297 P A PP R Sbjct: 353 PSPPARRHRSPPPAR 367 >At1g16610.2 68414.m01990 arginine/serine-rich protein, putative (SR45) similar to arginine/serine-rich protein GI:6601502 from [Arabidopsis thaliana] Length = 407 Score = 33.1 bits (72), Expect = 0.081 Identities = 23/66 (34%), Positives = 26/66 (39%), Gaps = 1/66 (1%) Frame = +1 Query: 103 GGERRSFGGDERRRSPARESYRKPSGXXPPRHPRIGTRPL-RPFRGRTLRSRHPIAAHRA 279 G R G RR PA S + PPR R R R RG +R R P+ R Sbjct: 248 GSPIRRRGDTPPRRRPASPSRGRSPSSPPPRRYRSPPRGSPRRIRGSPVRRRSPLPLRRR 307 Query: 280 RVPPLR 297 PP R Sbjct: 308 SPPPRR 313 >At1g16610.1 68414.m01989 arginine/serine-rich protein, putative (SR45) similar to arginine/serine-rich protein GI:6601502 from [Arabidopsis thaliana] Length = 414 Score = 33.1 bits (72), Expect = 0.081 Identities = 23/66 (34%), Positives = 26/66 (39%), Gaps = 1/66 (1%) Frame = +1 Query: 103 GGERRSFGGDERRRSPARESYRKPSGXXPPRHPRIGTRPL-RPFRGRTLRSRHPIAAHRA 279 G R G RR PA S + PPR R R R RG +R R P+ R Sbjct: 255 GSPIRRRGDTPPRRRPASPSRGRSPSSPPPRRYRSPPRGSPRRIRGSPVRRRSPLPLRRR 314 Query: 280 RVPPLR 297 PP R Sbjct: 315 SPPPRR 320 >At1g78230.1 68414.m09116 leucine-rich repeat family protein Length = 676 Score = 29.5 bits (63), Expect = 1.00 Identities = 15/37 (40%), Positives = 21/37 (56%), Gaps = 4/37 (10%) Frame = +3 Query: 327 NRAFRSLHGRS----SIKSKEEASSTEEDWDADDKEE 425 NR F H S+KSKEE + T E+ D +D++E Sbjct: 164 NREFSKFHVHERAVESVKSKEEVTDTSENGDDNDEDE 200 >At3g63030.1 68416.m07080 methyl-CpG-binding domain-containing protein contains Pfam profile PF01429: Methyl-CpG binding domain Length = 186 Score = 28.3 bits (60), Expect = 2.3 Identities = 20/51 (39%), Positives = 26/51 (50%) Frame = +1 Query: 235 GRTLRSRHPIAAHRARVPPLRGGSLIEPSITTVPLEASMDAVPSNPKKKHP 387 G+ LRSR+ IAA P R L + + TVP + D VP +PK P Sbjct: 119 GKKLRSRNEIAAFVEANPEFRNAPLGDFNF-TVP-KVMEDTVPPDPKLGSP 167 >At2g47350.1 68415.m05911 PAPA-1-like family protein / zinc finger (HIT type) family protein contains Pfam domains, PF04795: PAPA-1-like conserved region and PF04438: HIT zinc finger Length = 486 Score = 28.3 bits (60), Expect = 2.3 Identities = 11/34 (32%), Positives = 19/34 (55%) Frame = +1 Query: 169 KPSGXXPPRHPRIGTRPLRPFRGRTLRSRHPIAA 270 KPSG PPR +G P++ R +++ P+ + Sbjct: 438 KPSGYPPPRENCVGPSCTNPYKYRDSKTKVPLCS 471 >At4g12610.1 68417.m01987 transcription initiation factor IIF alpha subunit (TFIIF-alpha) family protein low similarity to SP|Q05913 Transcription initiation factor IIF, alpha subunit (TFIIF-alpha) (Transcription factor 5, large chain) (TF5A) {Drosophila melanogaster}; contains Pfam profile PF05793: Transcription initiation factor IIF, alpha subunit (TFIIF-alpha) Length = 543 Score = 27.9 bits (59), Expect = 3.0 Identities = 15/50 (30%), Positives = 23/50 (46%) Frame = +1 Query: 91 QENYGGERRSFGGDERRRSPARESYRKPSGXXPPRHPRIGTRPLRPFRGR 240 + NYG S + + P + KP+ PPR GT P +P +G+ Sbjct: 370 ETNYGTVTNSKQKEAAKEEPVDNAPAKPAPSGPPR----GTPPAKPSKGK 415 >At5g63920.1 68418.m08026 DNA topoisomerase III alpha, putative similar to Swiss-Prot:Q9NG98 DNA topoisomerase III alpha [Drosophila melanogaster] Length = 926 Score = 27.5 bits (58), Expect = 4.0 Identities = 17/58 (29%), Positives = 25/58 (43%), Gaps = 3/58 (5%) Frame = -2 Query: 264 DRMPRAQSAATEGAERAGADARVSRGXXTAWFAVAFSGGR---SSSFIPTEGPPFASV 100 D + + AT G+ G+ R SRG SGGR +SF+ G P + + Sbjct: 843 DSINNSSGNATTGSNSGGSGRRGSRGRGRGGRGGQSSGGRRGSGTSFVSATGEPVSGI 900 >At1g69485.1 68414.m07984 hypothetical protein Length = 125 Score = 27.1 bits (57), Expect = 5.3 Identities = 15/44 (34%), Positives = 21/44 (47%) Frame = -2 Query: 147 RSSSFIPTEGPPFASVIFLP*AVGPAVMTAAAFMATSFGWSLHL 16 R S++ PP S I A P V+ F++ SFG S+ L Sbjct: 22 RCFSYVAAISPPLDSAIDRSTASPPLVLPEFGFLSFSFGGSMEL 65 >At5g54260.1 68418.m06759 DNA repair and meiosis protein (Mre11) identical to DNA repair and meiosis protein (Mre11) GI:5524769 from [Arabidopsis thaliana] Length = 720 Score = 26.2 bits (55), Expect = 9.3 Identities = 15/56 (26%), Positives = 26/56 (46%) Frame = +3 Query: 258 SYRGTPRSRASFTRRFADRTLNYNRAFRSLHGRSSIKSKEEASSTEEDWDADDKEE 425 S +G R R + T+R R ++ R SS+ + +ED D +D+E+ Sbjct: 647 STKGKGRKRPATTKRGRGRGSGTSKRGRKNESSSSLNRLLSSKDDDEDEDDEDREK 702 >At5g53500.1 68418.m06649 WD-40 repeat family protein contains Pfam PF00400: WD domain, G-beta repeat; similar to WD-repeat protein 5 (WD repeat protein BIG-3) (SP: Q9UGP9) [Homo sapiens] Length = 654 Score = 26.2 bits (55), Expect = 9.3 Identities = 16/42 (38%), Positives = 21/42 (50%), Gaps = 4/42 (9%) Frame = +1 Query: 130 DERRRSPARESYRKPSGXX----PPRHPRIGTRPLRPFRGRT 243 +E + ES+RK S PP+ RI +PL FRG T Sbjct: 287 NEEKPKKTTESFRKTSDSACVVFPPKVFRIMEKPLYEFRGHT 328 >At3g07220.1 68416.m00861 transcriptional activator, putative similar to transcriptional activator FHA1 [Nicotiana tabacum] gi|15705932|gb|AAL05884 Length = 320 Score = 26.2 bits (55), Expect = 9.3 Identities = 13/35 (37%), Positives = 18/35 (51%) Frame = +3 Query: 321 NYNRAFRSLHGRSSIKSKEEASSTEEDWDADDKEE 425 NY S G+ ++S+E +ED D DD EE Sbjct: 142 NYQSGPGSGSGKKGVRSRELYEYDDEDDDDDDDEE 176 >At1g44850.1 68414.m05138 hypothetical protein Length = 266 Score = 26.2 bits (55), Expect = 9.3 Identities = 12/30 (40%), Positives = 15/30 (50%) Frame = +1 Query: 136 RRRSPARESYRKPSGXXPPRHPRIGTRPLR 225 R SP R +R PS P R + +PLR Sbjct: 15 RPMSPTRHYWRPPSALAPVRGNQFAVQPLR 44 >At1g14610.1 68414.m01737 valyl-tRNA synthetase / valine--tRNA ligase (VALRS) nearly identical to SP|P93736 Valyl-tRNA synthetase (EC 6.1.1.9) (Valine--tRNA ligase) (ValRS) {Arabidopsis thaliana} Length = 1108 Score = 26.2 bits (55), Expect = 9.3 Identities = 14/41 (34%), Positives = 21/41 (51%) Frame = +3 Query: 303 FADRTLNYNRAFRSLHGRSSIKSKEEASSTEEDWDADDKEE 425 F TL N+ RS HG ++ E+ TEE+ + K+E Sbjct: 26 FLSPTLT-NQLVRSFHGSRTMSESEKKILTEEELERKKKKE 65 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.316 0.135 0.400 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 7,738,503 Number of Sequences: 28952 Number of extensions: 137045 Number of successful extensions: 623 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 594 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 619 length of database: 12,070,560 effective HSP length: 74 effective length of database: 9,928,112 effective search space used: 665183504 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.6 bits)
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