Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00954
         (664 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|ch...   169   2e-43
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc...    38   0.002
SPAC824.08 |gda1|gdp1|guanosine-diphosphatase Gda1|Schizosacchar...    28   1.0  
SPBC776.05 |||membrane transporter |Schizosaccharomyces pombe|ch...    28   1.0  
SPBC3B9.16c |nup120||nucleoporin Nup120|Schizosaccharomyces pomb...    25   7.4  
SPAC6G10.05c |||TRAPP complex subunit Trs120 |Schizosaccharomyce...    25   9.7  

>SPBC2G2.12 |||histidine-tRNA ligase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 538

 Score =  169 bits (412), Expect = 2e-43
 Identities = 84/136 (61%), Positives = 101/136 (74%), Gaps = 1/136 (0%)
 Frame = +3

Query: 258 IDTPVFELKDVLTGKYGEDSKLIYDLKDQGGEILSLRYDLTVPLARYLAMN-KITTLKRY 434
           +DTPVFELK++L+GKYGEDSKLIYDLKDQGGE+ SLRYDLTVP AR+LAMN K+T++KR+
Sbjct: 99  LDTPVFELKEILSGKYGEDSKLIYDLKDQGGELCSLRYDLTVPFARWLAMNPKVTSIKRF 158

Query: 435 HIAKVYRRDNPAMTRGRYREFYQCDLT*LVYMILWYLMQNA*R**QRS*KLLT*GNISSK 614
           HIAKVYRRD PAMT+GR REFYQCD               A +    +   L  GN + K
Sbjct: 159 HIAKVYRRDQPAMTKGRMREFYQCDFDIAGNYDPMIPDSEALKVLVEALDALEVGNYTIK 218

Query: 615 WNHRRLLDGMFEACGV 662
            NHR++LDG+F  CGV
Sbjct: 219 LNHRKILDGIFTVCGV 234



 Score = 66.9 bits (156), Expect = 2e-12
 Identities = 36/89 (40%), Positives = 56/89 (62%), Gaps = 5/89 (5%)
 Frame = +1

Query: 10  KIQEQGDLVRKLKAAKDSNERVKEEVSKLLALKAQLV--TEDAGPQK---FTLKTPKGTR 174
           +I  QG+LVR LK+   S E + +EV+KLL LK   +  +E +G +K   FTLKTPKGT+
Sbjct: 11  RITSQGNLVRSLKSQGASKEDIDKEVAKLLQLKNLKLGGSEVSGKKKDTSFTLKTPKGTK 70

Query: 175 DYNPQQMTIRNNVLDKIITVFRRHGADVL 261
           D+  + + +R ++   +  +F+RHG   L
Sbjct: 71  DWCDKDVVLREHIFSTVTEIFKRHGGVTL 99



 Score = 58.4 bits (135), Expect = 9e-10
 Identities = 24/41 (58%), Positives = 33/41 (80%)
 Frame = +2

Query: 509 FDIAGVYDPMVPDAECLKVVTEILEALDIGQYILKVEPQKI 631
           FDIAG YDPM+PD+E LKV+ E L+AL++G Y +K+  +KI
Sbjct: 184 FDIAGNYDPMIPDSEALKVLVEALDALEVGNYTIKLNHRKI 224


>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
            |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1576

 Score = 37.5 bits (83), Expect = 0.002
 Identities = 19/54 (35%), Positives = 30/54 (55%)
 Frame = +3

Query: 294  TGKYGEDSKLIYDLKDQGGEILSLRYDLTVPLARYLAMNKITTLKRYHIAKVYR 455
            + +Y +D   +  L D+ G +L L YD  +P AR +A N +   K Y I+ V+R
Sbjct: 1015 SSQYDKDQASV-SLLDKNGTLLQLPYDTVLPYARNVARNAVEEEKTYLISDVFR 1067


>SPAC824.08 |gda1|gdp1|guanosine-diphosphatase
           Gda1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 556

 Score = 28.3 bits (60), Expect = 1.0
 Identities = 18/46 (39%), Positives = 24/46 (52%)
 Frame = -1

Query: 397 YRAKGTVKSYLNERISPPWSFKS*INFESSPYFPVSTSFSSKTGVS 260
           YR++G V   LN    P    +  + F   P FPV+TS  +KT VS
Sbjct: 57  YRSEGEVVDLLN----PKDELEEPL-FSEEPLFPVTTSIPTKTAVS 97


>SPBC776.05 |||membrane transporter |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 404

 Score = 28.3 bits (60), Expect = 1.0
 Identities = 12/33 (36%), Positives = 20/33 (60%)
 Frame = -2

Query: 240 PKNCNNFIQYIITYCHLLWVVVSCSLWCFKSKL 142
           P+N   F+  II Y + +  ++ CSLW + +KL
Sbjct: 318 PQNLQPFVFMIIQYLYSITTMLPCSLW-YNNKL 349


>SPBC3B9.16c |nup120||nucleoporin Nup120|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1136

 Score = 25.4 bits (53), Expect = 7.4
 Identities = 14/52 (26%), Positives = 27/52 (51%)
 Frame = +3

Query: 273 FELKDVLTGKYGEDSKLIYDLKDQGGEILSLRYDLTVPLARYLAMNKITTLK 428
           +E K +L  ++   +KL+  L   G EILS+ +D +  +      NK+  ++
Sbjct: 492 YEYKRLLYNEWERFAKLVAYLDHFGDEILSINFDPSNAVTYINYANKVAFIR 543


>SPAC6G10.05c |||TRAPP complex subunit Trs120 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1210

 Score = 25.0 bits (52), Expect = 9.7
 Identities = 17/38 (44%), Positives = 20/38 (52%)
 Frame = -2

Query: 282 LAQKQVYQYISSVSPKNCNNFIQYIITYCHLLWVVVSC 169
           L QK   Q   +V P  C  F + I+ Y HLL VV SC
Sbjct: 349 LYQKSTLQPNDAV-PGLC--FSESILRYAHLLTVVYSC 383


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,783,839
Number of Sequences: 5004
Number of extensions: 58575
Number of successful extensions: 154
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 144
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 152
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 301829700
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)