BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00954 (664 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g02760.1 68416.m00268 histidyl-tRNA synthetase, putative / hi... 153 1e-37 At3g46100.1 68416.m04988 histidyl-tRNA synthetase / histidine--t... 44 9e-05 At3g59410.1 68416.m06626 protein kinase family protein low simil... 43 2e-04 At2g31970.1 68415.m03906 DNA repair-recombination protein (RAD50... 33 0.13 At5g35940.1 68418.m04325 jacalin lectin family protein similar t... 29 2.8 At4g34450.1 68417.m04896 coatomer gamma-2 subunit, putative / ga... 29 3.6 At3g19020.1 68416.m02415 leucine-rich repeat family protein / ex... 29 3.6 At5g26780.3 68418.m03195 glycine hydroxymethyltransferase, putat... 28 6.4 At5g26780.2 68418.m03194 glycine hydroxymethyltransferase, putat... 28 6.4 At5g26780.1 68418.m03193 glycine hydroxymethyltransferase, putat... 28 6.4 At5g06670.1 68418.m00753 kinesin motor protein-related 28 6.4 At3g66652.1 68416.m00776 fip1 motif-containing protein contains ... 28 6.4 At5g56680.1 68418.m07075 asparaginyl-tRNA synthetase 1, cytoplas... 27 8.4 >At3g02760.1 68416.m00268 histidyl-tRNA synthetase, putative / histidine--tRNA ligase, putative similar to SP|P12081 Histidyl-tRNA synthetase (EC 6.1.1.21) (Histidine--tRNA ligase) (HisRS) {Homo sapiens}; contains Pfam profiles PF00587: tRNA synthetase class II core domain (G, H, P, S and T), PF03129: Anticodon binding domain Length = 479 Score = 153 bits (371), Expect = 1e-37 Identities = 79/135 (58%), Positives = 92/135 (68%) Frame = +3 Query: 258 IDTPVFELKDVLTGKYGEDSKLIYDLKDQGGEILSLRYDLTVPLARYLAMNKITTLKRYH 437 +DTPVFEL++ L GKYGEDSKL+YD+ DQGGE+ SLRYDLTVP ARY+AMN IT+ KRY Sbjct: 78 LDTPVFELRETLMGKYGEDSKLVYDIADQGGELCSLRYDLTVPFARYVAMNGITSFKRYQ 137 Query: 438 IAKVYRRDNPAMTRGRYREFYQCDLT*LVYMILWYLMQNA*R**QRS*KLLT*GNISSKW 617 IAKVYRRDNP ++GRYREFYQCD + L G+ K Sbjct: 138 IAKVYRRDNP--SKGRYREFYQCDFDIAGLFEPMGPDFEIVKILTELLDELEIGDYEVKL 195 Query: 618 NHRRLLDGMFEACGV 662 NHR+LLDGM E CGV Sbjct: 196 NHRKLLDGMLEICGV 210 Score = 50.8 bits (116), Expect = 8e-07 Identities = 20/41 (48%), Positives = 33/41 (80%) Frame = +2 Query: 509 FDIAGVYDPMVPDAECLKVVTEILEALDIGQYILKVEPQKI 631 FDIAG+++PM PD E +K++TE+L+ L+IG Y +K+ +K+ Sbjct: 160 FDIAGLFEPMGPDFEIVKILTELLDELEIGDYEVKLNHRKL 200 Score = 42.3 bits (95), Expect = 3e-04 Identities = 21/49 (42%), Positives = 26/49 (53%) Frame = +1 Query: 154 KTPKGTRDYNPQQMTIRNNVLDKIITVFRRHGADVLIHLFLS*RMCLLG 300 K PKGTRD+ +QM +R I VF+RHGA L R L+G Sbjct: 43 KLPKGTRDFAKEQMAVREKAFSIIQNVFKRHGATALDTPVFELRETLMG 91 >At3g46100.1 68416.m04988 histidyl-tRNA synthetase / histidine--tRNA ligase identical to histidyl-tRNA synthetase [Arabidopsis thaliana] GI:3659909 Length = 486 Score = 44.0 bits (99), Expect = 9e-05 Identities = 30/89 (33%), Positives = 49/89 (55%), Gaps = 4/89 (4%) Frame = +3 Query: 258 IDTPVFELKDVLTGKYGEDSK-LIYDLKDQGGEILSLRYDLTVPLARYLAMNKITT---L 425 +D PV E + + K GE+ + +Y +D+G ++LR +LT LAR + + L Sbjct: 105 VDYPVLETEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKSVSLPL 164 Query: 426 KRYHIAKVYRRDNPAMTRGRYREFYQCDL 512 K + I + +R + MTRGR RE YQ ++ Sbjct: 165 KWFAIGQCWRYER--MTRGRRREHYQWNM 191 Score = 39.9 bits (89), Expect = 0.001 Identities = 19/50 (38%), Positives = 30/50 (60%) Frame = +1 Query: 100 ALKAQLVTEDAGPQKFTLKTPKGTRDYNPQQMTIRNNVLDKIITVFRRHG 249 ++ A LVTE+ +K + PKGTRD+ P+ M +RN + + V R +G Sbjct: 52 SIVAPLVTEEDFHKKIDVNPPKGTRDFPPEDMRLRNWLFNHFKEVSRLYG 101 >At3g59410.1 68416.m06626 protein kinase family protein low similarity to GCN2 eIF2alpha kinase [Mus musculus] GI:6066585; contains Pfam profiles PF03129: Anticodon binding domain, PF00069: Protein kinase domain Length = 1241 Score = 42.7 bits (96), Expect = 2e-04 Identities = 18/48 (37%), Positives = 31/48 (64%) Frame = +3 Query: 315 SKLIYDLKDQGGEILSLRYDLTVPLARYLAMNKITTLKRYHIAKVYRR 458 S+ L GG++L L Y+L +P ++++N+ ++ KRY I+ VYRR Sbjct: 831 SRKTVKLLTNGGDMLELCYELRLPFVHWISVNQKSSFKRYEISHVYRR 878 >At2g31970.1 68415.m03906 DNA repair-recombination protein (RAD50) identical to DNA repair-recombination protein GI:7110148 from [Arabidopsis thaliana] Length = 1316 Score = 33.5 bits (73), Expect = 0.13 Identities = 25/71 (35%), Positives = 38/71 (53%) Frame = +1 Query: 1 LLLKIQEQGDLVRKLKAAKDSNERVKEEVSKLLALKAQLVTEDAGPQKFTLKTPKGTRDY 180 L +KIQE + + K KD+ R + SKL ALK + VT DA P+ L++ K RD Sbjct: 612 LQMKIQEVNNSL--FKHNKDTESRKRYIESKLQALKQESVTIDAYPK--LLESAKDKRDD 667 Query: 181 NPQQMTIRNNV 213 ++ + N + Sbjct: 668 RKREYNMANGM 678 >At5g35940.1 68418.m04325 jacalin lectin family protein similar to myrosinase-binding protein homolog [Arabidopsis thaliana] GI:2997767; contains Pfam profile PF01419 jacalin-like lectin domain Length = 444 Score = 29.1 bits (62), Expect = 2.8 Identities = 13/41 (31%), Positives = 21/41 (51%) Frame = -1 Query: 364 NERISPPWSFKS*INFESSPYFPVSTSFSSKTGVSIHQLRV 242 N+R SPP+ ++ +NF T F K+ +HQ+ V Sbjct: 397 NKRTSPPFGLEAGVNFVLQKESHKITGFHGKSSTMLHQIGV 437 >At4g34450.1 68417.m04896 coatomer gamma-2 subunit, putative / gamma-2 coat protein, putative / gamma-2 COP, putative similar to SP|Q9UBF2 Coatomer gamma-2 subunit (Gamma-2 coat protein) (Gamma-2 COP) {Homo sapiens}; contains Pfam profile: PF01602 Adaptin N terminal region Length = 886 Score = 28.7 bits (61), Expect = 3.6 Identities = 12/34 (35%), Positives = 20/34 (58%) Frame = +2 Query: 530 DPMVPDAECLKVVTEILEALDIGQYILKVEPQKI 631 DP V C +V+T++L L+ G+ KVE ++ Sbjct: 38 DPQVDPRRCSQVITKLLYLLNQGESFTKVEATEV 71 >At3g19020.1 68416.m02415 leucine-rich repeat family protein / extensin family protein similar to extensin-like protein [Lycopersicon esculentum] gi|5917664|gb|AAD55979; contains leucine-rich repeats, Pfam:PF00560; contains proline rich extensin domains, INTERPRO:IPR002965 Length = 956 Score = 28.7 bits (61), Expect = 3.6 Identities = 13/48 (27%), Positives = 26/48 (54%) Frame = -2 Query: 654 KLQTFHQVIFCGSTLRIYCPMSRASRISVTTFKHSASGTIGSYTPAMS 511 +++ ++++F G+ L P S +VT F S++G +GS +S Sbjct: 260 QMKNLNEIVFIGNNLSGCLPNEIGSLNNVTVFDASSNGFVGSLPSTLS 307 >At5g26780.3 68418.m03195 glycine hydroxymethyltransferase, putative / serine hydroxymethyltransferase, putative / serine/threonine aldolase, putative strong similarity to SP|P50433 Serine hydroxymethyltransferase, mitochondrial precursor (EC 2.1.2.1) (Serine methylase) (Glycine hydroxymethyltransferase) (SHMT) {Solanum tuberosum}; contains Pfam profile PF00464: serine hydroxymethyltransferase Length = 533 Score = 27.9 bits (59), Expect = 6.4 Identities = 12/32 (37%), Positives = 20/32 (62%) Frame = +1 Query: 1 LLLKIQEQGDLVRKLKAAKDSNERVKEEVSKL 96 L +K + QG ++ A SNE+++ E+SKL Sbjct: 477 LKIKAESQGTKLKDFVATMQSNEKLQSEMSKL 508 >At5g26780.2 68418.m03194 glycine hydroxymethyltransferase, putative / serine hydroxymethyltransferase, putative / serine/threonine aldolase, putative strong similarity to SP|P50433 Serine hydroxymethyltransferase, mitochondrial precursor (EC 2.1.2.1) (Serine methylase) (Glycine hydroxymethyltransferase) (SHMT) {Solanum tuberosum}; contains Pfam profile PF00464: serine hydroxymethyltransferase Length = 533 Score = 27.9 bits (59), Expect = 6.4 Identities = 12/32 (37%), Positives = 20/32 (62%) Frame = +1 Query: 1 LLLKIQEQGDLVRKLKAAKDSNERVKEEVSKL 96 L +K + QG ++ A SNE+++ E+SKL Sbjct: 477 LKIKAESQGTKLKDFVATMQSNEKLQSEMSKL 508 >At5g26780.1 68418.m03193 glycine hydroxymethyltransferase, putative / serine hydroxymethyltransferase, putative / serine/threonine aldolase, putative strong similarity to SP|P50433 Serine hydroxymethyltransferase, mitochondrial precursor (EC 2.1.2.1) (Serine methylase) (Glycine hydroxymethyltransferase) (SHMT) {Solanum tuberosum}; contains Pfam profile PF00464: serine hydroxymethyltransferase Length = 517 Score = 27.9 bits (59), Expect = 6.4 Identities = 12/32 (37%), Positives = 20/32 (62%) Frame = +1 Query: 1 LLLKIQEQGDLVRKLKAAKDSNERVKEEVSKL 96 L +K + QG ++ A SNE+++ E+SKL Sbjct: 461 LKIKAESQGTKLKDFVATMQSNEKLQSEMSKL 492 >At5g06670.1 68418.m00753 kinesin motor protein-related Length = 992 Score = 27.9 bits (59), Expect = 6.4 Identities = 15/54 (27%), Positives = 30/54 (55%), Gaps = 1/54 (1%) Frame = +1 Query: 10 KIQEQGDLVRKL-KAAKDSNERVKEEVSKLLALKAQLVTEDAGPQKFTLKTPKG 168 K+ E+ ++L AA + + EE+++L+ +L + A QK ++ TP+G Sbjct: 833 KLAEESSYAKELASAAAIELKALSEEIARLMNHNERLAADLAAVQKSSVTTPQG 886 >At3g66652.1 68416.m00776 fip1 motif-containing protein contains Pfam profile PF05182: Fip1 motif Length = 980 Score = 27.9 bits (59), Expect = 6.4 Identities = 8/27 (29%), Positives = 20/27 (74%) Frame = +1 Query: 16 QEQGDLVRKLKAAKDSNERVKEEVSKL 96 +E+G+++ ++K + NER++E + K+ Sbjct: 909 KEEGEIIEEVKGVEIDNERIQESLKKM 935 >At5g56680.1 68418.m07075 asparaginyl-tRNA synthetase 1, cytoplasmic / asparagine-tRNA ligase 1 (SYNC1) identical to SP|Q9SW96 Length = 572 Score = 27.5 bits (58), Expect = 8.4 Identities = 14/34 (41%), Positives = 22/34 (64%) Frame = +1 Query: 7 LKIQEQGDLVRKLKAAKDSNERVKEEVSKLLALK 108 L + E+G++V +LKAAK S E + V++L K Sbjct: 242 LIVIERGNVVAELKAAKASKEAITAAVAELKIAK 275 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,096,064 Number of Sequences: 28952 Number of extensions: 285750 Number of successful extensions: 686 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 670 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 685 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1393347168 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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