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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00954
         (664 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g02760.1 68416.m00268 histidyl-tRNA synthetase, putative / hi...   153   1e-37
At3g46100.1 68416.m04988 histidyl-tRNA synthetase / histidine--t...    44   9e-05
At3g59410.1 68416.m06626 protein kinase family protein low simil...    43   2e-04
At2g31970.1 68415.m03906 DNA repair-recombination protein (RAD50...    33   0.13 
At5g35940.1 68418.m04325 jacalin lectin family protein similar t...    29   2.8  
At4g34450.1 68417.m04896 coatomer gamma-2 subunit, putative / ga...    29   3.6  
At3g19020.1 68416.m02415 leucine-rich repeat family protein / ex...    29   3.6  
At5g26780.3 68418.m03195 glycine hydroxymethyltransferase, putat...    28   6.4  
At5g26780.2 68418.m03194 glycine hydroxymethyltransferase, putat...    28   6.4  
At5g26780.1 68418.m03193 glycine hydroxymethyltransferase, putat...    28   6.4  
At5g06670.1 68418.m00753 kinesin motor protein-related                 28   6.4  
At3g66652.1 68416.m00776 fip1 motif-containing protein contains ...    28   6.4  
At5g56680.1 68418.m07075 asparaginyl-tRNA synthetase 1, cytoplas...    27   8.4  

>At3g02760.1 68416.m00268 histidyl-tRNA synthetase, putative /
           histidine--tRNA ligase, putative similar to SP|P12081
           Histidyl-tRNA synthetase (EC 6.1.1.21) (Histidine--tRNA
           ligase) (HisRS) {Homo sapiens}; contains Pfam profiles
           PF00587: tRNA synthetase class II core domain (G, H, P,
           S and T), PF03129: Anticodon binding domain
          Length = 479

 Score =  153 bits (371), Expect = 1e-37
 Identities = 79/135 (58%), Positives = 92/135 (68%)
 Frame = +3

Query: 258 IDTPVFELKDVLTGKYGEDSKLIYDLKDQGGEILSLRYDLTVPLARYLAMNKITTLKRYH 437
           +DTPVFEL++ L GKYGEDSKL+YD+ DQGGE+ SLRYDLTVP ARY+AMN IT+ KRY 
Sbjct: 78  LDTPVFELRETLMGKYGEDSKLVYDIADQGGELCSLRYDLTVPFARYVAMNGITSFKRYQ 137

Query: 438 IAKVYRRDNPAMTRGRYREFYQCDLT*LVYMILWYLMQNA*R**QRS*KLLT*GNISSKW 617
           IAKVYRRDNP  ++GRYREFYQCD                 +        L  G+   K 
Sbjct: 138 IAKVYRRDNP--SKGRYREFYQCDFDIAGLFEPMGPDFEIVKILTELLDELEIGDYEVKL 195

Query: 618 NHRRLLDGMFEACGV 662
           NHR+LLDGM E CGV
Sbjct: 196 NHRKLLDGMLEICGV 210



 Score = 50.8 bits (116), Expect = 8e-07
 Identities = 20/41 (48%), Positives = 33/41 (80%)
 Frame = +2

Query: 509 FDIAGVYDPMVPDAECLKVVTEILEALDIGQYILKVEPQKI 631
           FDIAG+++PM PD E +K++TE+L+ L+IG Y +K+  +K+
Sbjct: 160 FDIAGLFEPMGPDFEIVKILTELLDELEIGDYEVKLNHRKL 200



 Score = 42.3 bits (95), Expect = 3e-04
 Identities = 21/49 (42%), Positives = 26/49 (53%)
 Frame = +1

Query: 154 KTPKGTRDYNPQQMTIRNNVLDKIITVFRRHGADVLIHLFLS*RMCLLG 300
           K PKGTRD+  +QM +R      I  VF+RHGA  L       R  L+G
Sbjct: 43  KLPKGTRDFAKEQMAVREKAFSIIQNVFKRHGATALDTPVFELRETLMG 91


>At3g46100.1 68416.m04988 histidyl-tRNA synthetase / histidine--tRNA
           ligase identical to histidyl-tRNA synthetase
           [Arabidopsis thaliana] GI:3659909
          Length = 486

 Score = 44.0 bits (99), Expect = 9e-05
 Identities = 30/89 (33%), Positives = 49/89 (55%), Gaps = 4/89 (4%)
 Frame = +3

Query: 258 IDTPVFELKDVLTGKYGEDSK-LIYDLKDQGGEILSLRYDLTVPLARYLAMNKITT---L 425
           +D PV E + +   K GE+ +  +Y  +D+G   ++LR +LT  LAR +     +    L
Sbjct: 105 VDYPVLETEALFIRKAGEEIRDQLYCFEDRGNRRVALRPELTPSLARLVIQKGKSVSLPL 164

Query: 426 KRYHIAKVYRRDNPAMTRGRYREFYQCDL 512
           K + I + +R +   MTRGR RE YQ ++
Sbjct: 165 KWFAIGQCWRYER--MTRGRRREHYQWNM 191



 Score = 39.9 bits (89), Expect = 0.001
 Identities = 19/50 (38%), Positives = 30/50 (60%)
 Frame = +1

Query: 100 ALKAQLVTEDAGPQKFTLKTPKGTRDYNPQQMTIRNNVLDKIITVFRRHG 249
           ++ A LVTE+   +K  +  PKGTRD+ P+ M +RN + +    V R +G
Sbjct: 52  SIVAPLVTEEDFHKKIDVNPPKGTRDFPPEDMRLRNWLFNHFKEVSRLYG 101


>At3g59410.1 68416.m06626 protein kinase family protein low
           similarity to GCN2 eIF2alpha kinase [Mus musculus]
           GI:6066585; contains Pfam profiles PF03129: Anticodon
           binding domain, PF00069: Protein kinase domain
          Length = 1241

 Score = 42.7 bits (96), Expect = 2e-04
 Identities = 18/48 (37%), Positives = 31/48 (64%)
 Frame = +3

Query: 315 SKLIYDLKDQGGEILSLRYDLTVPLARYLAMNKITTLKRYHIAKVYRR 458
           S+    L   GG++L L Y+L +P   ++++N+ ++ KRY I+ VYRR
Sbjct: 831 SRKTVKLLTNGGDMLELCYELRLPFVHWISVNQKSSFKRYEISHVYRR 878


>At2g31970.1 68415.m03906 DNA repair-recombination protein (RAD50)
           identical to DNA repair-recombination protein GI:7110148
           from [Arabidopsis thaliana]
          Length = 1316

 Score = 33.5 bits (73), Expect = 0.13
 Identities = 25/71 (35%), Positives = 38/71 (53%)
 Frame = +1

Query: 1   LLLKIQEQGDLVRKLKAAKDSNERVKEEVSKLLALKAQLVTEDAGPQKFTLKTPKGTRDY 180
           L +KIQE  + +   K  KD+  R +   SKL ALK + VT DA P+   L++ K  RD 
Sbjct: 612 LQMKIQEVNNSL--FKHNKDTESRKRYIESKLQALKQESVTIDAYPK--LLESAKDKRDD 667

Query: 181 NPQQMTIRNNV 213
             ++  + N +
Sbjct: 668 RKREYNMANGM 678


>At5g35940.1 68418.m04325 jacalin lectin family protein similar to
           myrosinase-binding protein homolog [Arabidopsis
           thaliana] GI:2997767; contains Pfam profile PF01419
           jacalin-like lectin domain
          Length = 444

 Score = 29.1 bits (62), Expect = 2.8
 Identities = 13/41 (31%), Positives = 21/41 (51%)
 Frame = -1

Query: 364 NERISPPWSFKS*INFESSPYFPVSTSFSSKTGVSIHQLRV 242
           N+R SPP+  ++ +NF         T F  K+   +HQ+ V
Sbjct: 397 NKRTSPPFGLEAGVNFVLQKESHKITGFHGKSSTMLHQIGV 437


>At4g34450.1 68417.m04896 coatomer gamma-2 subunit, putative /
           gamma-2 coat protein, putative / gamma-2 COP, putative
           similar to SP|Q9UBF2 Coatomer gamma-2 subunit (Gamma-2
           coat protein) (Gamma-2 COP) {Homo sapiens}; contains
           Pfam profile: PF01602 Adaptin N terminal region
          Length = 886

 Score = 28.7 bits (61), Expect = 3.6
 Identities = 12/34 (35%), Positives = 20/34 (58%)
 Frame = +2

Query: 530 DPMVPDAECLKVVTEILEALDIGQYILKVEPQKI 631
           DP V    C +V+T++L  L+ G+   KVE  ++
Sbjct: 38  DPQVDPRRCSQVITKLLYLLNQGESFTKVEATEV 71


>At3g19020.1 68416.m02415 leucine-rich repeat family protein /
           extensin family protein similar to extensin-like protein
           [Lycopersicon esculentum] gi|5917664|gb|AAD55979;
           contains leucine-rich repeats, Pfam:PF00560; contains
           proline rich extensin domains, INTERPRO:IPR002965
          Length = 956

 Score = 28.7 bits (61), Expect = 3.6
 Identities = 13/48 (27%), Positives = 26/48 (54%)
 Frame = -2

Query: 654 KLQTFHQVIFCGSTLRIYCPMSRASRISVTTFKHSASGTIGSYTPAMS 511
           +++  ++++F G+ L    P    S  +VT F  S++G +GS    +S
Sbjct: 260 QMKNLNEIVFIGNNLSGCLPNEIGSLNNVTVFDASSNGFVGSLPSTLS 307


>At5g26780.3 68418.m03195 glycine hydroxymethyltransferase, putative
           / serine hydroxymethyltransferase, putative /
           serine/threonine aldolase, putative strong similarity to
           SP|P50433 Serine hydroxymethyltransferase, mitochondrial
           precursor (EC 2.1.2.1) (Serine methylase) (Glycine
           hydroxymethyltransferase) (SHMT) {Solanum tuberosum};
           contains Pfam profile PF00464: serine
           hydroxymethyltransferase
          Length = 533

 Score = 27.9 bits (59), Expect = 6.4
 Identities = 12/32 (37%), Positives = 20/32 (62%)
 Frame = +1

Query: 1   LLLKIQEQGDLVRKLKAAKDSNERVKEEVSKL 96
           L +K + QG  ++   A   SNE+++ E+SKL
Sbjct: 477 LKIKAESQGTKLKDFVATMQSNEKLQSEMSKL 508


>At5g26780.2 68418.m03194 glycine hydroxymethyltransferase, putative
           / serine hydroxymethyltransferase, putative /
           serine/threonine aldolase, putative strong similarity to
           SP|P50433 Serine hydroxymethyltransferase, mitochondrial
           precursor (EC 2.1.2.1) (Serine methylase) (Glycine
           hydroxymethyltransferase) (SHMT) {Solanum tuberosum};
           contains Pfam profile PF00464: serine
           hydroxymethyltransferase
          Length = 533

 Score = 27.9 bits (59), Expect = 6.4
 Identities = 12/32 (37%), Positives = 20/32 (62%)
 Frame = +1

Query: 1   LLLKIQEQGDLVRKLKAAKDSNERVKEEVSKL 96
           L +K + QG  ++   A   SNE+++ E+SKL
Sbjct: 477 LKIKAESQGTKLKDFVATMQSNEKLQSEMSKL 508


>At5g26780.1 68418.m03193 glycine hydroxymethyltransferase, putative
           / serine hydroxymethyltransferase, putative /
           serine/threonine aldolase, putative strong similarity to
           SP|P50433 Serine hydroxymethyltransferase, mitochondrial
           precursor (EC 2.1.2.1) (Serine methylase) (Glycine
           hydroxymethyltransferase) (SHMT) {Solanum tuberosum};
           contains Pfam profile PF00464: serine
           hydroxymethyltransferase
          Length = 517

 Score = 27.9 bits (59), Expect = 6.4
 Identities = 12/32 (37%), Positives = 20/32 (62%)
 Frame = +1

Query: 1   LLLKIQEQGDLVRKLKAAKDSNERVKEEVSKL 96
           L +K + QG  ++   A   SNE+++ E+SKL
Sbjct: 461 LKIKAESQGTKLKDFVATMQSNEKLQSEMSKL 492


>At5g06670.1 68418.m00753 kinesin motor protein-related 
          Length = 992

 Score = 27.9 bits (59), Expect = 6.4
 Identities = 15/54 (27%), Positives = 30/54 (55%), Gaps = 1/54 (1%)
 Frame = +1

Query: 10  KIQEQGDLVRKL-KAAKDSNERVKEEVSKLLALKAQLVTEDAGPQKFTLKTPKG 168
           K+ E+    ++L  AA    + + EE+++L+    +L  + A  QK ++ TP+G
Sbjct: 833 KLAEESSYAKELASAAAIELKALSEEIARLMNHNERLAADLAAVQKSSVTTPQG 886


>At3g66652.1 68416.m00776 fip1 motif-containing protein contains
           Pfam profile PF05182: Fip1 motif
          Length = 980

 Score = 27.9 bits (59), Expect = 6.4
 Identities = 8/27 (29%), Positives = 20/27 (74%)
 Frame = +1

Query: 16  QEQGDLVRKLKAAKDSNERVKEEVSKL 96
           +E+G+++ ++K  +  NER++E + K+
Sbjct: 909 KEEGEIIEEVKGVEIDNERIQESLKKM 935


>At5g56680.1 68418.m07075 asparaginyl-tRNA synthetase 1, cytoplasmic
           / asparagine-tRNA ligase 1 (SYNC1) identical to
           SP|Q9SW96
          Length = 572

 Score = 27.5 bits (58), Expect = 8.4
 Identities = 14/34 (41%), Positives = 22/34 (64%)
 Frame = +1

Query: 7   LKIQEQGDLVRKLKAAKDSNERVKEEVSKLLALK 108
           L + E+G++V +LKAAK S E +   V++L   K
Sbjct: 242 LIVIERGNVVAELKAAKASKEAITAAVAELKIAK 275


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,096,064
Number of Sequences: 28952
Number of extensions: 285750
Number of successful extensions: 686
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 670
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 685
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1393347168
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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