Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00949
(684 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g13950.1 68414.m01639 eukaryotic translation initiation facto... 105 4e-23
At1g69410.1 68414.m07972 eukaryotic translation initiation facto... 102 3e-22
At1g26630.1 68414.m03243 eukaryotic translation initiation facto... 101 3e-22
At3g06450.1 68416.m00746 anion exchange family protein similar t... 31 0.94
At1g56345.1 68414.m06477 pseudouridine synthase family protein l... 29 2.2
At5g14230.1 68418.m01663 ankyrin repeat family protein contains ... 29 2.9
At5g03140.1 68418.m00262 lectin protein kinase family protein co... 29 3.8
At3g51290.1 68416.m05614 proline-rich family protein 28 5.0
At2g27680.1 68415.m03354 aldo/keto reductase family protein cont... 28 5.0
At5g13880.1 68418.m01622 expressed protein 27 8.8
At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-... 27 8.8
At3g59820.1 68416.m06675 calcium-binding mitochondrial protein-r... 27 8.8
At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative simila... 27 8.8
At2g28550.1 68415.m03468 AP2 domain-containing transcription fac... 27 8.8
At1g35830.1 68414.m04452 VQ motif-containing protein contains PF... 27 8.8
>At1g13950.1 68414.m01639 eukaryotic translation initiation factor
5A-1 / eIF-5A 1 identical to SP|Q9XI91 Eukaryotic
translation initiation factor 5A-1 (eIF-5A 1)
{Arabidopsis thaliana}
Length = 158
Score = 105 bits (251), Expect = 4e-23
Identities = 46/83 (55%), Positives = 63/83 (75%), Gaps = 1/83 (1%)
Frame = +3
Query: 255 FNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGD-LGT 431
F KK EDI PS+HN DVPHV R DYQL DIS+DGY++L+ DNG ++DLK+P+ D L
Sbjct: 65 FTSKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKDDLKLPNDDTLLQ 124
Query: 432 QLRTDFDSGKELLCTVLKSCGEE 500
Q+++ FD GK+L+ +V+ + GEE
Sbjct: 125 QIKSGFDDGKDLVVSVMSAMGEE 147
Score = 101 bits (241), Expect = 6e-22
Identities = 42/72 (58%), Positives = 55/72 (76%)
Frame = +1
Query: 73 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 252
E+ HFE+ D+GAS T+P Q +RKNG++++K RPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 EEHHFESSDAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 253 ISMVKSMKISVP 288
I K ++ VP
Sbjct: 64 IFTSKKLEDIVP 75
>At1g69410.1 68414.m07972 eukaryotic translation initiation factor
5A, putative / eIF-5A, putative strong similarity to
eukaryotic initiation factor 5A (2) (Nicotiana
plumbaginifolia) GI:19702, SP|Q9AXQ6| Eukaryotic
translation initiation factor 5A-1 (eIF-5A 1)
{Lycopersicon esculentum}
Length = 158
Score = 102 bits (244), Expect = 3e-22
Identities = 46/90 (51%), Positives = 65/90 (72%), Gaps = 1/90 (1%)
Frame = +3
Query: 255 FNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGT 431
F KK EDI PS+HN DVPHV R DYQL DIS+DG+++L+ DNG ++DLK+P D L T
Sbjct: 65 FTSKKLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLT 124
Query: 432 QLRTDFDSGKELLCTVLKSCGEECVMPSKQ 521
QL+ F+ GK+++ +V+ + GEE + K+
Sbjct: 125 QLKNGFEEGKDIVVSVMSAMGEEQMCALKE 154
Score = 97.5 bits (232), Expect = 7e-21
Identities = 41/72 (56%), Positives = 54/72 (75%)
Frame = +1
Query: 73 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 252
++ HFE+ D+GAS T+P Q +RK G +++KGRPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 DEHHFESSDAGASKTYPQQAGNIRKGGHIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 253 ISMVKSMKISVP 288
I K ++ VP
Sbjct: 64 IFTSKKLEDIVP 75
>At1g26630.1 68414.m03243 eukaryotic translation initiation factor
5A, putative / eIF-5A, putative strong similariy to
SP|Q9AXQ6 Eukaryotic translation initiation factor 5A-1
(eIF-5A 1) {Lycopersicon esculentum}
Length = 159
Score = 101 bits (243), Expect = 3e-22
Identities = 45/90 (50%), Positives = 64/90 (71%), Gaps = 1/90 (1%)
Frame = +3
Query: 255 FNGKKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGT 431
F KK EDI PS+HN DVPHV R DYQL DI++DG+++L+ D+G ++DLK+P D L
Sbjct: 65 FTAKKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTDDGLTA 124
Query: 432 QLRTDFDSGKELLCTVLKSCGEECVMPSKQ 521
Q+R FD GK+++ +V+ S GEE + K+
Sbjct: 125 QMRLGFDEGKDIVVSVMSSMGEEQICAVKE 154
Score = 93.1 bits (221), Expect = 2e-19
Identities = 39/72 (54%), Positives = 51/72 (70%)
Frame = +1
Query: 73 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 252
++ HFE +SGAS T+P +RK G +++K RPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4 DEHHFEASESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63
Query: 253 ISMVKSMKISVP 288
I K ++ VP
Sbjct: 64 IFTAKKLEDIVP 75
>At3g06450.1 68416.m00746 anion exchange family protein similar to
putative Anion exchanger family members: GB:AAD39673,
GB:AAD55295 [Arabidopsis thaliana]
Length = 732
Score = 30.7 bits (66), Expect = 0.94
Identities = 12/21 (57%), Positives = 13/21 (61%)
Frame = -1
Query: 675 LRAPPLYYYSFFKLNLYTILC 613
LR PP Y+Y F L TILC
Sbjct: 328 LRKPPAYHYDLFLLGFLTILC 348
>At1g56345.1 68414.m06477 pseudouridine synthase family protein low
similarity to SP|P23851 Ribosomal large subunit
pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate
synthase) (Uracil hydrolyase) {Escherichia coli};
contains Pfam profile PF00849: RNA pseudouridylate
synthase
Length = 322
Score = 29.5 bits (63), Expect = 2.2
Identities = 20/69 (28%), Positives = 35/69 (50%), Gaps = 1/69 (1%)
Frame = -2
Query: 482 QHGAQQFLAAVEVSTQLCAEVTIRDFEVLTQ-VTVVSHQGQVAIIRDISQLVVFALHVGY 306
+HGA + AA++V L +RD E + V+V S + + + D++ ++V G
Sbjct: 176 KHGAWRVYAALDVGRVLPGGSFVRDMETTFEVVSVNSVKNESCELEDVNHVIVAE---GE 232
Query: 305 VHVVCGGTD 279
+ CGG D
Sbjct: 233 RELSCGGDD 241
>At5g14230.1 68418.m01663 ankyrin repeat family protein contains
ankyrin repeats, Pfam:PF00023
Length = 591
Score = 29.1 bits (62), Expect = 2.9
Identities = 15/34 (44%), Positives = 16/34 (47%)
Frame = +3
Query: 402 LKIPDGDLGTQLRTDFDSGKELLCTVLKSCGEEC 503
L IPDGD T L G +C L SCG C
Sbjct: 412 LDIPDGDGYTPLMLAAREGHGHMCEYLISCGANC 445
>At5g03140.1 68418.m00262 lectin protein kinase family protein
contains Pfam domains, PF00138: Legume lectins alpha
domain, PF00139: Legume lectins beta domain and PF00069:
Protein kinase domain
Length = 711
Score = 28.7 bits (61), Expect = 3.8
Identities = 13/36 (36%), Positives = 21/36 (58%)
Frame = -2
Query: 173 RPFSITKPFLRRAEHCMGKVAEAPESPVSKCVSSMS 66
+P +T+P +R + A+ PE P++K SSMS
Sbjct: 631 QPDPVTRPTMRSVVQILVGEADVPEVPIAKPSSSMS 666
>At3g51290.1 68416.m05614 proline-rich family protein
Length = 602
Score = 28.3 bits (60), Expect = 5.0
Identities = 10/25 (40%), Positives = 14/25 (56%)
Frame = +2
Query: 50 KTQQWVTSKTHTLRPETPGPQPPSP 124
+T W T+ T ++ P P P PP P
Sbjct: 91 ETTTWTTTTTSSVLPPPPPPPPPPP 115
>At2g27680.1 68415.m03354 aldo/keto reductase family protein
contains Pfam profile PF00248: oxidoreductase, aldo/keto
reductase family
Length = 384
Score = 28.3 bits (60), Expect = 5.0
Identities = 26/95 (27%), Positives = 44/95 (46%)
Frame = +3
Query: 273 EDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDLGTQLRTDFD 452
++I S MDV + + D ++DGYL + DL+E +G + T T+FD
Sbjct: 147 QNIDISRKRMDVAALDMLQFHWWDYANDGYLDALKHLTDLKE-----EGKIKTVALTNFD 201
Query: 453 SGKELLCTVLKSCGEECVMPSKQTQLSTNKPTQHL 557
+ E L +L++ G V Q + +P Q +
Sbjct: 202 T--ERLQKILEN-GIPVVSNQVQHSIVDMRPQQRM 233
>At5g13880.1 68418.m01622 expressed protein
Length = 188
Score = 27.5 bits (58), Expect = 8.8
Identities = 9/36 (25%), Positives = 19/36 (52%)
Frame = +2
Query: 2 VEEPVGHISFLTVVKFKTQQWVTSKTHTLRPETPGP 109
+E+ + ++ L + +F Q W+ + P+TP P
Sbjct: 150 LEDEIDKVADLFITRFHRQMWLQKQLSLESPDTPSP 185
>At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-box
RNA helicase [Chlamydomonas reinhardtii] GI:12044832;
contains Pfam profiles PF04408: Helicase associated
domain (HA2), PF00271: Helicase conserved C-terminal
domain
Length = 1226
Score = 27.5 bits (58), Expect = 8.8
Identities = 15/51 (29%), Positives = 28/51 (54%), Gaps = 1/51 (1%)
Frame = +3
Query: 315 VKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDL-GTQLRTDFDSGKE 464
V+R+ +++ Y L ADN + + G + GT+++T+FDS +E
Sbjct: 350 VRRDGSKMSLAQSKKYSQLNADNAQWEDRQLLRSGAVRGTEVQTEFDSEEE 400
>At3g59820.1 68416.m06675 calcium-binding mitochondrial
protein-related contains weak similarity to
Calcium-binding mitochondrial protein Anon-60Da
(Swiss-Prot:P91927) [Drosophila melanogaster]
Length = 755
Score = 27.5 bits (58), Expect = 8.8
Identities = 16/44 (36%), Positives = 21/44 (47%)
Frame = +2
Query: 131 VRPCVKTVSLC*RVVHARLLKCPHPKPESTATLKFTWLGLIFQW 262
+ P +K V+ R A+ K H K E +TLK WLG W
Sbjct: 182 IGPAIKAVASMNRADWAK--KLTHWKHEFVSTLKHYWLGTKLLW 223
>At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative similar to
SP|P35207 Antiviral protein SKI2 {Saccharomyces
cerevisiae}; contains Pfam profiles PF00270: DEAD/DEAH
box helicase, PF00271: Helicase conserved C-terminal
domain
Length = 1347
Score = 27.5 bits (58), Expect = 8.8
Identities = 17/51 (33%), Positives = 30/51 (58%), Gaps = 6/51 (11%)
Frame = +3
Query: 345 ISDDGYLTLMADNG--DLREDLKIPDGDLGTQLR----TDFDSGKELLCTV 479
+SD+ L + A G D+ +++ D DL Q++ + +SG+EL+CTV
Sbjct: 1141 MSDEALLQMPAFQGRIDVLKNIGCIDDDLVVQIKGRVACEMNSGEELICTV 1191
>At2g28550.1 68415.m03468 AP2 domain-containing transcription factor
RAP2.7 (RAP2.7) nearly identical to AP2 domain
transcription factor RAP2.7 (GI:2281639) [Arabidopsis
thaliana]
Length = 449
Score = 27.5 bits (58), Expect = 8.8
Identities = 9/15 (60%), Positives = 12/15 (80%)
Frame = +2
Query: 74 KTHTLRPETPGPQPP 118
+T +RP+ PGPQPP
Sbjct: 402 QTSAVRPQPPGPQPP 416
>At1g35830.1 68414.m04452 VQ motif-containing protein contains
PF05678: VQ motif
Length = 302
Score = 27.5 bits (58), Expect = 8.8
Identities = 11/33 (33%), Positives = 17/33 (51%)
Frame = +2
Query: 26 SFLTVVKFKTQQWVTSKTHTLRPETPGPQPPSP 124
++L V + ++TH L P+ P P PP P
Sbjct: 60 TYLNFVNNLISDDILNQTHLLPPQPPPPPPPPP 92
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,807,962
Number of Sequences: 28952
Number of extensions: 353461
Number of successful extensions: 1027
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 953
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1017
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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