Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00939
(710 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g27670.1 68417.m03979 25.3 kDa small heat shock protein, chlo... 44 1e-04
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 44 1e-04
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 44 1e-04
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 42 3e-04
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 42 4e-04
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 40 0.001
At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein (HS... 40 0.002
At1g52560.1 68414.m05933 26.5 kDa class I small heat shock prote... 34 0.11
At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock pro... 31 0.75
At5g56930.1 68418.m07107 zinc finger (CCCH-type) family protein ... 30 1.7
At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family prote... 29 3.0
At5g60030.1 68418.m07527 expressed protein 28 7.0
At5g50630.1 68418.m06272 nodulin family protein similar to nodul... 28 7.0
At5g50520.1 68418.m06257 nodulin family protein similar to nodul... 28 7.0
At1g31440.1 68414.m03850 SH3 domain-containing protein 1 (SH3P1)... 28 7.0
At5g64200.2 68418.m08063 arginine/serine-rich splicing factor SC... 27 9.3
At5g64200.1 68418.m08062 arginine/serine-rich splicing factor SC... 27 9.3
At1g53330.1 68414.m06045 pentatricopeptide (PPR) repeat-containi... 27 9.3
At1g35160.1 68414.m04360 14-3-3 protein GF14 phi (GRF4) identica... 27 9.3
At1g27680.1 68414.m03383 glucose-1-phosphate adenylyltransferase... 27 9.3
>At4g27670.1 68417.m03979 25.3 kDa small heat shock protein,
chloroplast precursor (HSP25.3-P) identical to small
heat shock protein, chloroplast precursor SP:P31170 from
[Arabidopsis thaliana]; identified in Scharf, K-D., et
al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 227
Score = 44.0 bits (99), Expect = 1e-04
Identities = 20/84 (23%), Positives = 42/84 (50%), Gaps = 3/84 (3%)
Frame = +3
Query: 249 IPVNLDVQHFSPEEISVKTADGYVIVEGKHEERQDEHGYISRQ---FTRRYALPENCNPD 419
I + D+ S E++ + D ++++G+ ++ + + R + R LP+NC D
Sbjct: 138 IKMRFDMPGLSKEDVKISVEDNVLVIKGEQKKEDSDDSWSGRSVSSYGTRLQLPDNCEKD 197
Query: 420 TVESRLSSDGVLTVIAPRTPAATK 491
+++ L +GVL + P+T K
Sbjct: 198 KIKAEL-KNGVLFITIPKTKVERK 220
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 43.6 bits (98), Expect = 1e-04
Identities = 25/80 (31%), Positives = 46/80 (57%), Gaps = 8/80 (10%)
Frame = +3
Query: 264 DVQHFSPEEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFTRRYALPENCNPD 419
D+ EE+ V+ DG ++ + G+ +EE+ D+ + R +FTRR+ LPEN +
Sbjct: 66 DLPGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENAKME 125
Query: 420 TVESRLSSDGVLTVIAPRTP 479
+++ + +GVL+V P+ P
Sbjct: 126 EIKASM-ENGVLSVTVPKVP 144
>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
(HSP17.8-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]; contains Pfam
PF00011: Hsp20/alpha crystallin family
Length = 157
Score = 43.6 bits (98), Expect = 1e-04
Identities = 29/84 (34%), Positives = 45/84 (53%), Gaps = 8/84 (9%)
Frame = +3
Query: 264 DVQHFSPEEISVKTADGYVI-VEGKH----EERQDEHGYISR---QFTRRYALPENCNPD 419
D+ EE+ V+ D V+ + G+ EE+QD + R QF+R++ LPEN D
Sbjct: 64 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMD 123
Query: 420 TVESRLSSDGVLTVIAPRTPAATK 491
V++ + +GVLTV P+ A K
Sbjct: 124 QVKASM-ENGVLTVTVPKVEEAKK 146
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 42.3 bits (95), Expect = 3e-04
Identities = 26/80 (32%), Positives = 45/80 (56%), Gaps = 8/80 (10%)
Frame = +3
Query: 264 DVQHFSPEEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFTRRYALPENCNPD 419
D+ EE+ V+ D V+ + G+ +EE+ D+ + R +F RR+ LPEN +
Sbjct: 68 DLPGLKKEEVKVEVEDKNVLQISGERSKENEEKNDKWHRVERASGKFMRRFRLPENAKME 127
Query: 420 TVESRLSSDGVLTVIAPRTP 479
V++ + +GVLTV+ P+ P
Sbjct: 128 EVKATM-ENGVLTVVVPKAP 146
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 41.9 bits (94), Expect = 4e-04
Identities = 27/89 (30%), Positives = 48/89 (53%), Gaps = 8/89 (8%)
Frame = +3
Query: 264 DVQHFSPEEISVKTADGYVI-VEG-KHEERQDEHGYISR------QFTRRYALPENCNPD 419
D+ EE+ V+ + V+ + G +H E++D++ R QFTRR+ LPEN D
Sbjct: 62 DLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMD 121
Query: 420 TVESRLSSDGVLTVIAPRTPAATKNERAV 506
V++ + +GVLTV P+ + +++
Sbjct: 122 QVKAAM-ENGVLTVTVPKAETKKADVKSI 149
>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
(HSP17.4-CI) identical to 17.4 kDa class I heat shock
protein SP:P19036 from [Arabidopsis thaliana]
Length = 156
Score = 40.3 bits (90), Expect = 0.001
Identities = 26/89 (29%), Positives = 47/89 (52%), Gaps = 8/89 (8%)
Frame = +3
Query: 264 DVQHFSPEEISVKTADGYVI-VEGK----HEERQDEHGYISR---QFTRRYALPENCNPD 419
DV EE+ V+ DG ++ + G+ +EE+ D + R +F RR+ LPEN +
Sbjct: 65 DVPGLKKEEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFMRRFRLPENAKVE 124
Query: 420 TVESRLSSDGVLTVIAPRTPAATKNERAV 506
V++ + +GVL+V P+ + ++V
Sbjct: 125 EVKASM-ENGVLSVTVPKVQESKPEVKSV 152
>At1g59860.1 68414.m06742 17.6 kDa class I heat shock protein
(HSP17.6A-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]
Length = 155
Score = 39.5 bits (88), Expect = 0.002
Identities = 27/84 (32%), Positives = 43/84 (51%), Gaps = 8/84 (9%)
Frame = +3
Query: 264 DVQHFSPEEISVKTADGYVI-VEGKH----EERQDEHGYISRQ---FTRRYALPENCNPD 419
D+ EE+ V+ D V+ + G+ EE+QD + R F+R++ LPEN D
Sbjct: 62 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGGFSRKFRLPENVKMD 121
Query: 420 TVESRLSSDGVLTVIAPRTPAATK 491
V++ + +GVLTV P+ K
Sbjct: 122 QVKASM-ENGVLTVTVPKVETNKK 144
>At1g52560.1 68414.m05933 26.5 kDa class I small heat shock
protein-like (HSP26.5-P) contains Pfam profile: PF00011
Hsp20/alpha crystallin family; identified in Scharf,
K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 232
Score = 33.9 bits (74), Expect = 0.11
Identities = 20/90 (22%), Positives = 45/90 (50%), Gaps = 9/90 (10%)
Frame = +3
Query: 264 DVQHFSPEEISVKTADGYVIVEGKHEERQD-----EHGYISRQ----FTRRYALPENCNP 416
+V + E++ + DG + ++G H+ ++ E Y S + + +LP++
Sbjct: 141 EVPGLTKEDVKITVNDGILTIKGDHKAEEEKGSPEEDEYWSSKSYGYYNTSLSLPDDAKV 200
Query: 417 DTVESRLSSDGVLTVIAPRTPAATKNERAV 506
+ +++ L + GVL ++ PRT KN + +
Sbjct: 201 EDIKAELKN-GVLNLVIPRTEKPKKNVQEI 229
>At4g21870.1 68417.m03163 26.5 kDa class P-related heat shock
protein (HSP26.5-P) contains Pfam profile: PF00011
Hsp20/alpha crystallin family: identified in Scharf,
K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 134
Score = 31.1 bits (67), Expect = 0.75
Identities = 19/72 (26%), Positives = 33/72 (45%)
Frame = +3
Query: 258 NLDVQHFSPEEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRL 437
++D+ EEI V+ D ++ + F R++ LPE+ + + +
Sbjct: 40 SVDLPGLRKEEIKVEIEDSIYLIIRTEATPMSPPDQPLKTFKRKFRLPESIDMIGISAGY 99
Query: 438 SSDGVLTVIAPR 473
DGVLTVI P+
Sbjct: 100 E-DGVLTVIVPK 110
>At5g56930.1 68418.m07107 zinc finger (CCCH-type) family protein
contains Pfam domain, PF00642: Zinc finger
C-x8-C-x5-C-x3-H type (and similar)
Length = 675
Score = 29.9 bits (64), Expect = 1.7
Identities = 16/53 (30%), Positives = 26/53 (49%), Gaps = 3/53 (5%)
Frame = +3
Query: 468 PRTPAATKNERAVPSL---KPARSGRRLRSPLRKLRATKQNNDSRNAVNQKSV 617
P+ PA++KN + PS S +L + + + NND AVN+ +V
Sbjct: 594 PQKPASSKNLKTTPSSHIQSSLLSAMKLAAEFESAKVERGNNDPTEAVNKSNV 646
>At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 822
Score = 29.1 bits (62), Expect = 3.0
Identities = 26/118 (22%), Positives = 42/118 (35%)
Frame = +3
Query: 285 EEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDGVLTVI 464
+EI D Y EG EE ++ R + L E+C + VES D
Sbjct: 48 DEIDSAYGDFYEYEEGVPEEESRKNNRYERVVNYEFELAEDCEDENVESEDDDDDDDDDD 107
Query: 465 APRTPAATKNERAVPSLKPARSGRRLRSPLRKLRATKQNNDSRNAVNQKSVIACVNFI 638
R KN +P + R + + N +R+ + KS+ +F+
Sbjct: 108 DDRHSRMLKNVTELPISAFQGESKNKRVVFTEPYPESEFNPTRDVLEGKSLTTIDDFM 165
>At5g60030.1 68418.m07527 expressed protein
Length = 292
Score = 27.9 bits (59), Expect = 7.0
Identities = 18/50 (36%), Positives = 27/50 (54%), Gaps = 6/50 (12%)
Frame = +2
Query: 452 ADRDRSQDSGCHEERASCSITQTGPVRKEIK----EPTA--EAESNETKQ 583
+D SQD + RAS + T+ +EIK +P++ E +S ETKQ
Sbjct: 29 SDNGASQDVSAYLRRASAAFTELKSFHREIKSKETKPSSDRETKSTETKQ 78
>At5g50630.1 68418.m06272 nodulin family protein similar to
nodulin-like protein [Arabidopsis thaliana] GI:3329368,
nodule-specific protein Nlj70 [Lotus japonicus]
GI:3329366
Length = 540
Score = 27.9 bits (59), Expect = 7.0
Identities = 15/61 (24%), Positives = 28/61 (45%)
Frame = +2
Query: 506 SITQTGPVRKEIKEPTAEAESNETKQ*LSERCESKKCYSVC*FYLHSSCVLVRLIALSYL 685
S +G + I + A ++ T + S C CYSV + C++ +++LS +
Sbjct: 464 SFVFSGVIASNIYDYYARKQAGPTTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVV 523
Query: 686 Y 688
Y
Sbjct: 524 Y 524
>At5g50520.1 68418.m06257 nodulin family protein similar to
nodulin-like protein [Arabidopsis thaliana] GI:3329368,
nodule-specific protein Nlj70 [Lotus japonicus]
GI:3329366
Length = 540
Score = 27.9 bits (59), Expect = 7.0
Identities = 15/61 (24%), Positives = 28/61 (45%)
Frame = +2
Query: 506 SITQTGPVRKEIKEPTAEAESNETKQ*LSERCESKKCYSVC*FYLHSSCVLVRLIALSYL 685
S +G + I + A ++ T + S C CYSV + C++ +++LS +
Sbjct: 464 SFVFSGVIASNIYDYYARKQAGPTTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVV 523
Query: 686 Y 688
Y
Sbjct: 524 Y 524
>At1g31440.1 68414.m03850 SH3 domain-containing protein 1 (SH3P1)
nearly identical to SH3 domain-containing protein 1
[Arabidopsis thaliana] GI:16974676; contains Pfam
profile PF00018: SH3 domain
Length = 439
Score = 27.9 bits (59), Expect = 7.0
Identities = 16/38 (42%), Positives = 17/38 (44%)
Frame = +3
Query: 108 LRTGAYSGRSPDGRHHANDVQRLLPSVEADGDRQ*RRR 221
+RT YS D RH N RL VEA RRR
Sbjct: 136 IRTMIYSAPLEDARHLVNHYDRLRQEVEAQATDVLRRR 173
>At5g64200.2 68418.m08063 arginine/serine-rich splicing factor SC35
contains similarity to splicing factor; contains Pfam
profile PF00076: RNA recognition motif. (a.k.a. RRM,
RBD, or RNP domain)
Length = 303
Score = 27.5 bits (58), Expect = 9.3
Identities = 16/48 (33%), Positives = 23/48 (47%)
Frame = +3
Query: 471 RTPAATKNERAVPSLKPARSGRRLRSPLRKLRATKQNNDSRNAVNQKS 614
R+P +++ R S RS RR RSP R+ R + D R +S
Sbjct: 119 RSPRRSRSPRRSRSPPRRRSPRRSRSPRRRSRDDYREKDYRKRSRSRS 166
>At5g64200.1 68418.m08062 arginine/serine-rich splicing factor SC35
contains similarity to splicing factor; contains Pfam
profile PF00076: RNA recognition motif. (a.k.a. RRM,
RBD, or RNP domain)
Length = 303
Score = 27.5 bits (58), Expect = 9.3
Identities = 16/48 (33%), Positives = 23/48 (47%)
Frame = +3
Query: 471 RTPAATKNERAVPSLKPARSGRRLRSPLRKLRATKQNNDSRNAVNQKS 614
R+P +++ R S RS RR RSP R+ R + D R +S
Sbjct: 119 RSPRRSRSPRRSRSPPRRRSPRRSRSPRRRSRDDYREKDYRKRSRSRS 166
>At1g53330.1 68414.m06045 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 471
Score = 27.5 bits (58), Expect = 9.3
Identities = 21/74 (28%), Positives = 32/74 (43%)
Frame = +3
Query: 342 ERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDGVLTVIAPRTPAATKNERAVPSLKP 521
++ +E Y+ RR C+PDT+ R+ DG+ + A +E KP
Sbjct: 342 KKWEEATYLFEDMPRR-----GCSPDTLSYRIVFDGLCEGLQFEEAAVILDEMLFKGYKP 396
Query: 522 ARSGRRLRSPLRKL 563
R RL L+KL
Sbjct: 397 RRD--RLEGFLQKL 408
>At1g35160.1 68414.m04360 14-3-3 protein GF14 phi (GRF4) identical
to GF14 protein phi chain GI:1493805, SP:P46077 from
[Arabidopsis thaliana]
Length = 267
Score = 27.5 bits (58), Expect = 9.3
Identities = 13/46 (28%), Positives = 24/46 (52%)
Frame = +2
Query: 86 RTAFAIRTSDWRLLRTIS*RPSSRQRCPEITTVRGSRWRSPIETSV 223
+ R + WR++ +I + SR +TT+R +RS IE+ +
Sbjct: 59 KNVIGARRASWRIISSIEQKEESRGNDDHVTTIRD--YRSKIESEL 102
>At1g27680.1 68414.m03383 glucose-1-phosphate adenylyltransferase
large subunit 2 (APL2) / ADP-glucose pyrophosphorylase
identical to SP|P55230
Length = 518
Score = 27.5 bits (58), Expect = 9.3
Identities = 15/59 (25%), Positives = 26/59 (44%)
Frame = +3
Query: 285 EEISVKTADGYVIVEGKHEERQDEHGYISRQFTRRYALPENCNPDTVESRLSSDGVLTV 461
E+ K + +I+ G H R D ++ + + +C P ESR S G+L +
Sbjct: 198 EDAKTKNVEHVLILSGDHLYRMDYMNFVQKHIESNADITVSCLP-MDESRASDFGLLKI 255
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,182,151
Number of Sequences: 28952
Number of extensions: 282109
Number of successful extensions: 935
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 901
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 933
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1535986264
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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