SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00933
         (577 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC23D3.07 |pup1||20S proteasome component beta 2|Schizosacchar...   100   1e-22
SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyc...    50   3e-07
SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy...    36   0.006
SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy...    31   0.091
SPCC74.09 |mug24||RNA-binding protein, rrm type|Schizosaccharomy...    29   0.49 
SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Sc...    29   0.64 
SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ...    28   1.1  
SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces...    27   2.0  
SPBC30D10.15 |||snoRNP assembly factor |Schizosaccharomyces pomb...    26   4.5  
SPBC56F2.04 |utp20||U3 snoRNP protein Utp20|Schizosaccharomyces ...    26   4.5  
SPAC110.01 |ppk1|SPAC140.05|serine/threonine protein kinase Ppk1...    26   4.5  
SPAP27G11.14c |||sequence orphan|Schizosaccharomyces pombe|chr 1...    25   6.0  
SPAC2E1P3.05c |||fungal cellulose binding domain protein|Schizos...    25   6.0  

>SPAC23D3.07 |pup1||20S proteasome component beta
           2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 267

 Score =  100 bits (240), Expect = 1e-22
 Identities = 48/82 (58%), Positives = 59/82 (71%)
 Frame = +2

Query: 2   LQRLHTGRTVPVETAATLLKRMLFRYQGHIGAALVLGGVDRTGPHIYCIYPHGSVDKLPY 181
           L  L+T R   V TA T+LK+ LFRYQGHIGA LVLGG D  GPH++ I  HGS DKLPY
Sbjct: 100 LHSLYTNRKPRVVTALTMLKQHLFRYQGHIGAYLVLGGYDCKGPHLFTIAAHGSSDKLPY 159

Query: 182 ATMGSGSLAAMAVFEAGWKRDM 247
             +GSGSLAA++V E  ++ D+
Sbjct: 160 VALGSGSLAAISVLETKYQPDL 181



 Score = 69.3 bits (162), Expect = 4e-13
 Identities = 45/93 (48%), Positives = 55/93 (59%), Gaps = 6/93 (6%)
 Frame = +1

Query: 199 ISCGYGSV*--SRLET*Y----E*GEGKKLVRDAIAAGIFNDLGSGSNVDLCVIRNTGPA 360
           ++ G GS+   S LET Y    E  E  +LV++AI AGIFNDLGSGSN DL VI      
Sbjct: 160 VALGSGSLAAISVLETKYQPDLERHEAMELVKEAIEAGIFNDLGSGSNCDLVVIDEEKAT 219

Query: 361 QYLRTYEEANVKGKKQGSYRYALGTTAVLKQRV 459
            Y R Y + N +  KQ  Y Y  GTTAVLK+ +
Sbjct: 220 PY-RGYSKPNERATKQSKYTYDRGTTAVLKEDI 251


>SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 226

 Score = 49.6 bits (113), Expect = 3e-07
 Identities = 28/83 (33%), Positives = 43/83 (51%), Gaps = 1/83 (1%)
 Frame = +2

Query: 2   LQRLHTGRTVPVETAATLLKRMLFRYQGHIGAALVLGGVD-RTGPHIYCIYPHGSVDKLP 178
           + R+  G    V TAATL   M ++ +  + A L++ G D +TG  +Y I   GS+ K P
Sbjct: 89  MYRIQFGHDPSVHTAATLASEMCYQNKNMLSAGLIVAGYDEKTGGDVYSIPLGGSLHKQP 148

Query: 179 YATMGSGSLAAMAVFEAGWKRDM 247
            A  GSGS       +A ++ +M
Sbjct: 149 LAIGGSGSAFIYGFCDANFRENM 171


>SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 247

 Score = 35.5 bits (78), Expect = 0.006
 Identities = 17/52 (32%), Positives = 27/52 (51%)
 Frame = +2

Query: 92  GAALVLGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMAVFEAGWKRDM 247
           G AL++ G+D  GP +Y   P G+  +     +GSGS  A +     + +DM
Sbjct: 139 GVALLIAGIDEHGPQLYHSEPSGTYFRYEAKAIGSGSEPAKSELVKEFHKDM 190


>SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 245

 Score = 31.5 bits (68), Expect = 0.091
 Identities = 14/52 (26%), Positives = 25/52 (48%)
 Frame = +2

Query: 92  GAALVLGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMAVFEAGWKRDM 247
           G +L++ G+D  GP +Y + P G+        +G  S AA    E  +  ++
Sbjct: 131 GVSLLVAGMDEKGPSLYQVDPSGTYFAWKATAIGKSSTAAKTFLEKRYNDEL 182


>SPCC74.09 |mug24||RNA-binding protein, rrm type|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 654

 Score = 29.1 bits (62), Expect = 0.49
 Identities = 11/39 (28%), Positives = 18/39 (46%)
 Frame = -3

Query: 329 STLEPEPKSLNIPAAIASRTSFFPSPHSYHVSNLLQTLP 213
           S + P P S   P    S  +F P P++++  N+    P
Sbjct: 132 SVIYPSPPSTYYPNMYVSANTFIPMPYAHYTDNMYHAKP 170


>SPAC6G10.04c |||20S proteasome component alpha 6 subunit
           Pre5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 272

 Score = 28.7 bits (61), Expect = 0.64
 Identities = 15/45 (33%), Positives = 22/45 (48%)
 Frame = +2

Query: 92  GAALVLGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMAVFE 226
           G   ++ G D +GPH+    P G V +    +MGS S +A    E
Sbjct: 129 GVGFLVIGYDESGPHLLEFQPSGLVLEYLGTSMGSRSQSARTYIE 173


>SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 620

 Score = 27.9 bits (59), Expect = 1.1
 Identities = 12/33 (36%), Positives = 21/33 (63%)
 Frame = -1

Query: 163 RSMRINAVNMRTGSVNTSQNQSSPYMTLIAKQH 65
           +S+    + M+TGSVN +Q+Q    M ++A Q+
Sbjct: 267 QSIPSGPMGMKTGSVNDTQSQQITLMNVLASQN 299


>SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 995

 Score = 27.1 bits (57), Expect = 2.0
 Identities = 11/37 (29%), Positives = 20/37 (54%)
 Frame = -1

Query: 148 NAVNMRTGSVNTSQNQSSPYMTLIAKQHSFQQCGSSF 38
           N + M+  S+ T + + +PY  L+A     + CGS +
Sbjct: 861 NIIPMQKPSLTTHETKQNPYDLLVALDSDRKACGSLY 897


>SPBC30D10.15 |||snoRNP assembly factor |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 516

 Score = 25.8 bits (54), Expect = 4.5
 Identities = 17/61 (27%), Positives = 30/61 (49%)
 Frame = -2

Query: 381 FISS*ILGRASVTNNAQVNIRT*TQVIEYTSSNSISDKLFSFSSFISRFQPASNTAIAAR 202
           FIS+  +G  +  ++ + N++   ++ E T SNS   K  +  S  S     SNT+ +  
Sbjct: 145 FISNEEVGIPATNSSEKSNVKIADEIAEVTHSNSSIGKSSADLSSDSSSDSESNTSFSET 204

Query: 201 D 199
           D
Sbjct: 205 D 205


>SPBC56F2.04 |utp20||U3 snoRNP protein Utp20|Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 2493

 Score = 25.8 bits (54), Expect = 4.5
 Identities = 11/32 (34%), Positives = 22/32 (68%), Gaps = 2/32 (6%)
 Frame = -2

Query: 495  WAYQNRFY--LERYYTLLKHSSCTKSISVRAL 406
            WA+ N++   L+RY +LLK+++  + + VR +
Sbjct: 1525 WAHWNQYQAILKRYVSLLKNNAIDQKVVVRLI 1556


>SPAC110.01 |ppk1|SPAC140.05|serine/threonine protein kinase Ppk1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1023

 Score = 25.8 bits (54), Expect = 4.5
 Identities = 14/37 (37%), Positives = 20/37 (54%)
 Frame = -3

Query: 323 LEPEPKSLNIPAAIASRTSFFPSPHSYHVSNLLQTLP 213
           L  EPKS+ +P    SRT++  S  S  V N  + +P
Sbjct: 387 LYQEPKSIQLPELSVSRTTY--SAQSSSVKNCNERIP 421


>SPAP27G11.14c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 689

 Score = 25.4 bits (53), Expect = 6.0
 Identities = 13/40 (32%), Positives = 21/40 (52%)
 Frame = +1

Query: 376 YEEANVKGKKQGSYRYALGTTAVLKQRVIPLEVESILVRP 495
           YE  NV+  K+ +Y++      V+  ++I  E  SIL  P
Sbjct: 334 YETINVEETKKETYKFTRSIKTVIPNKLISNESISILSIP 373


>SPAC2E1P3.05c |||fungal cellulose binding domain
           protein|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 197

 Score = 25.4 bits (53), Expect = 6.0
 Identities = 14/47 (29%), Positives = 19/47 (40%)
 Frame = +3

Query: 396 GEKARLVQICSWYNCCA*AACNTSRGRIYFGTPNRSISPMDVEPQTS 536
           G  A+L Q C   N      C      IY G       P+D++P +S
Sbjct: 67  GPCAKLYQQCGGINYNGPTCCEPGSECIYNGPYYSQCIPVDIDPSSS 113


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,391,741
Number of Sequences: 5004
Number of extensions: 49231
Number of successful extensions: 147
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 139
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 146
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 246098644
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -