BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00933 (577 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC23D3.07 |pup1||20S proteasome component beta 2|Schizosacchar... 100 1e-22 SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyc... 50 3e-07 SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy... 36 0.006 SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy... 31 0.091 SPCC74.09 |mug24||RNA-binding protein, rrm type|Schizosaccharomy... 29 0.49 SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Sc... 29 0.64 SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 28 1.1 SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces... 27 2.0 SPBC30D10.15 |||snoRNP assembly factor |Schizosaccharomyces pomb... 26 4.5 SPBC56F2.04 |utp20||U3 snoRNP protein Utp20|Schizosaccharomyces ... 26 4.5 SPAC110.01 |ppk1|SPAC140.05|serine/threonine protein kinase Ppk1... 26 4.5 SPAP27G11.14c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 25 6.0 SPAC2E1P3.05c |||fungal cellulose binding domain protein|Schizos... 25 6.0 >SPAC23D3.07 |pup1||20S proteasome component beta 2|Schizosaccharomyces pombe|chr 1|||Manual Length = 267 Score = 100 bits (240), Expect = 1e-22 Identities = 48/82 (58%), Positives = 59/82 (71%) Frame = +2 Query: 2 LQRLHTGRTVPVETAATLLKRMLFRYQGHIGAALVLGGVDRTGPHIYCIYPHGSVDKLPY 181 L L+T R V TA T+LK+ LFRYQGHIGA LVLGG D GPH++ I HGS DKLPY Sbjct: 100 LHSLYTNRKPRVVTALTMLKQHLFRYQGHIGAYLVLGGYDCKGPHLFTIAAHGSSDKLPY 159 Query: 182 ATMGSGSLAAMAVFEAGWKRDM 247 +GSGSLAA++V E ++ D+ Sbjct: 160 VALGSGSLAAISVLETKYQPDL 181 Score = 69.3 bits (162), Expect = 4e-13 Identities = 45/93 (48%), Positives = 55/93 (59%), Gaps = 6/93 (6%) Frame = +1 Query: 199 ISCGYGSV*--SRLET*Y----E*GEGKKLVRDAIAAGIFNDLGSGSNVDLCVIRNTGPA 360 ++ G GS+ S LET Y E E +LV++AI AGIFNDLGSGSN DL VI Sbjct: 160 VALGSGSLAAISVLETKYQPDLERHEAMELVKEAIEAGIFNDLGSGSNCDLVVIDEEKAT 219 Query: 361 QYLRTYEEANVKGKKQGSYRYALGTTAVLKQRV 459 Y R Y + N + KQ Y Y GTTAVLK+ + Sbjct: 220 PY-RGYSKPNERATKQSKYTYDRGTTAVLKEDI 251 >SPBC4C3.10c |||20S proteasome component beta 1|Schizosaccharomyces pombe|chr 2|||Manual Length = 226 Score = 49.6 bits (113), Expect = 3e-07 Identities = 28/83 (33%), Positives = 43/83 (51%), Gaps = 1/83 (1%) Frame = +2 Query: 2 LQRLHTGRTVPVETAATLLKRMLFRYQGHIGAALVLGGVD-RTGPHIYCIYPHGSVDKLP 178 + R+ G V TAATL M ++ + + A L++ G D +TG +Y I GS+ K P Sbjct: 89 MYRIQFGHDPSVHTAATLASEMCYQNKNMLSAGLIVAGYDEKTGGDVYSIPLGGSLHKQP 148 Query: 179 YATMGSGSLAAMAVFEAGWKRDM 247 A GSGS +A ++ +M Sbjct: 149 LAIGGSGSAFIYGFCDANFRENM 171 >SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces pombe|chr 1|||Manual Length = 247 Score = 35.5 bits (78), Expect = 0.006 Identities = 17/52 (32%), Positives = 27/52 (51%) Frame = +2 Query: 92 GAALVLGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMAVFEAGWKRDM 247 G AL++ G+D GP +Y P G+ + +GSGS A + + +DM Sbjct: 139 GVALLIAGIDEHGPQLYHSEPSGTYFRYEAKAIGSGSEPAKSELVKEFHKDM 190 >SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces pombe|chr 3|||Manual Length = 245 Score = 31.5 bits (68), Expect = 0.091 Identities = 14/52 (26%), Positives = 25/52 (48%) Frame = +2 Query: 92 GAALVLGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMAVFEAGWKRDM 247 G +L++ G+D GP +Y + P G+ +G S AA E + ++ Sbjct: 131 GVSLLVAGMDEKGPSLYQVDPSGTYFAWKATAIGKSSTAAKTFLEKRYNDEL 182 >SPCC74.09 |mug24||RNA-binding protein, rrm type|Schizosaccharomyces pombe|chr 3|||Manual Length = 654 Score = 29.1 bits (62), Expect = 0.49 Identities = 11/39 (28%), Positives = 18/39 (46%) Frame = -3 Query: 329 STLEPEPKSLNIPAAIASRTSFFPSPHSYHVSNLLQTLP 213 S + P P S P S +F P P++++ N+ P Sbjct: 132 SVIYPSPPSTYYPNMYVSANTFIPMPYAHYTDNMYHAKP 170 >SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Schizosaccharomyces pombe|chr 1|||Manual Length = 272 Score = 28.7 bits (61), Expect = 0.64 Identities = 15/45 (33%), Positives = 22/45 (48%) Frame = +2 Query: 92 GAALVLGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMAVFE 226 G ++ G D +GPH+ P G V + +MGS S +A E Sbjct: 129 GVGFLVIGYDESGPHLLEFQPSGLVLEYLGTSMGSRSQSARTYIE 173 >SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 27.9 bits (59), Expect = 1.1 Identities = 12/33 (36%), Positives = 21/33 (63%) Frame = -1 Query: 163 RSMRINAVNMRTGSVNTSQNQSSPYMTLIAKQH 65 +S+ + M+TGSVN +Q+Q M ++A Q+ Sbjct: 267 QSIPSGPMGMKTGSVNDTQSQQITLMNVLASQN 299 >SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 995 Score = 27.1 bits (57), Expect = 2.0 Identities = 11/37 (29%), Positives = 20/37 (54%) Frame = -1 Query: 148 NAVNMRTGSVNTSQNQSSPYMTLIAKQHSFQQCGSSF 38 N + M+ S+ T + + +PY L+A + CGS + Sbjct: 861 NIIPMQKPSLTTHETKQNPYDLLVALDSDRKACGSLY 897 >SPBC30D10.15 |||snoRNP assembly factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 516 Score = 25.8 bits (54), Expect = 4.5 Identities = 17/61 (27%), Positives = 30/61 (49%) Frame = -2 Query: 381 FISS*ILGRASVTNNAQVNIRT*TQVIEYTSSNSISDKLFSFSSFISRFQPASNTAIAAR 202 FIS+ +G + ++ + N++ ++ E T SNS K + S S SNT+ + Sbjct: 145 FISNEEVGIPATNSSEKSNVKIADEIAEVTHSNSSIGKSSADLSSDSSSDSESNTSFSET 204 Query: 201 D 199 D Sbjct: 205 D 205 >SPBC56F2.04 |utp20||U3 snoRNP protein Utp20|Schizosaccharomyces pombe|chr 2|||Manual Length = 2493 Score = 25.8 bits (54), Expect = 4.5 Identities = 11/32 (34%), Positives = 22/32 (68%), Gaps = 2/32 (6%) Frame = -2 Query: 495 WAYQNRFY--LERYYTLLKHSSCTKSISVRAL 406 WA+ N++ L+RY +LLK+++ + + VR + Sbjct: 1525 WAHWNQYQAILKRYVSLLKNNAIDQKVVVRLI 1556 >SPAC110.01 |ppk1|SPAC140.05|serine/threonine protein kinase Ppk1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1023 Score = 25.8 bits (54), Expect = 4.5 Identities = 14/37 (37%), Positives = 20/37 (54%) Frame = -3 Query: 323 LEPEPKSLNIPAAIASRTSFFPSPHSYHVSNLLQTLP 213 L EPKS+ +P SRT++ S S V N + +P Sbjct: 387 LYQEPKSIQLPELSVSRTTY--SAQSSSVKNCNERIP 421 >SPAP27G11.14c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 689 Score = 25.4 bits (53), Expect = 6.0 Identities = 13/40 (32%), Positives = 21/40 (52%) Frame = +1 Query: 376 YEEANVKGKKQGSYRYALGTTAVLKQRVIPLEVESILVRP 495 YE NV+ K+ +Y++ V+ ++I E SIL P Sbjct: 334 YETINVEETKKETYKFTRSIKTVIPNKLISNESISILSIP 373 >SPAC2E1P3.05c |||fungal cellulose binding domain protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 197 Score = 25.4 bits (53), Expect = 6.0 Identities = 14/47 (29%), Positives = 19/47 (40%) Frame = +3 Query: 396 GEKARLVQICSWYNCCA*AACNTSRGRIYFGTPNRSISPMDVEPQTS 536 G A+L Q C N C IY G P+D++P +S Sbjct: 67 GPCAKLYQQCGGINYNGPTCCEPGSECIYNGPYYSQCIPVDIDPSSS 113 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,391,741 Number of Sequences: 5004 Number of extensions: 49231 Number of successful extensions: 147 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 139 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 146 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 246098644 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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