Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00933
(577 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2) (P... 118 2e-27
At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2) (P... 118 2e-27
At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1) id... 118 3e-27
At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1) id... 118 3e-27
At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1) (... 50 1e-06
At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative... 50 1e-06
At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1) (P... 44 6e-05
At2g27020.1 68415.m03244 20S proteasome alpha subunit G (PAG1) (... 37 0.008
At5g42790.1 68418.m05212 20S proteasome alpha subunit F1 (PAF1) ... 33 0.10
At1g47250.1 68414.m05231 20S proteasome alpha subunit F2 (PAF2) ... 33 0.10
At1g53850.1 68414.m06129 20S proteasome alpha subunit E1 (PAE1) ... 32 0.24
At1g79210.1 68414.m09235 20S proteasome alpha subunit B, putativ... 32 0.32
At1g16470.1 68414.m01970 20S proteasome alpha subunit B (PAB1) (... 32 0.32
At3g14290.1 68416.m01808 20S proteasome alpha subunit E2 (PAE2) ... 31 0.42
At1g79670.1 68414.m09291 wall-associated kinase, putative simila... 31 0.42
At3g51180.1 68416.m05603 zinc finger (CCCH-type) family protein ... 29 1.7
At1g77850.1 68414.m09072 transcriptional factor B3 family protei... 27 6.8
At5g23170.1 68418.m02710 protein kinase family protein contains ... 27 9.0
At1g55480.1 68414.m06346 expressed protein 27 9.0
>At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2)
(PRCFC) identical to 20S proteasome beta subunit PBB2
[Arabidopsis thaliana] GI:3421104, cDNA proteasome
subunit prcfc GI:2511575
Length = 274
Score = 118 bits (285), Expect = 2e-27
Identities = 56/82 (68%), Positives = 64/82 (78%)
Frame = +2
Query: 2 LQRLHTGRTVPVETAATLLKRMLFRYQGHIGAALVLGGVDRTGPHIYCIYPHGSVDKLPY 181
L R TGR V TA TLLK+ LF YQGH+ AALVLGGVD TGPH++ IYPHGS D LP+
Sbjct: 104 LHRYQTGRDSRVVTALTLLKKHLFSYQGHVSAALVLGGVDITGPHLHTIYPHGSTDTLPF 163
Query: 182 ATMGSGSLAAMAVFEAGWKRDM 247
ATMGSGSLAAM+VFEA +K +
Sbjct: 164 ATMGSGSLAAMSVFEAKYKEGL 185
Score = 63.3 bits (147), Expect = 1e-10
Identities = 35/71 (49%), Positives = 42/71 (59%)
Frame = +1
Query: 256 EGKKLVRDAIAAGIFNDLGSGSNVDLCVIRNTGPAQYLRTYEEANVKGKKQGSYRYALGT 435
EG KLV +AI +GIFNDLGSGSNVD+CVI G +YLR Y E N +
Sbjct: 189 EGIKLVAEAICSGIFNDLGSGSNVDICVI-TKGHKEYLRNYMEPNPRTYVSSKGYSFTKK 247
Query: 436 TAVLKQRVIPL 468
T VL ++ PL
Sbjct: 248 TEVLLTKITPL 258
>At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2)
(PRCFC) identical to 20S proteasome beta subunit PBB2
[Arabidopsis thaliana] GI:3421104, cDNA proteasome
subunit prcfc GI:2511575
Length = 274
Score = 118 bits (285), Expect = 2e-27
Identities = 56/82 (68%), Positives = 64/82 (78%)
Frame = +2
Query: 2 LQRLHTGRTVPVETAATLLKRMLFRYQGHIGAALVLGGVDRTGPHIYCIYPHGSVDKLPY 181
L R TGR V TA TLLK+ LF YQGH+ AALVLGGVD TGPH++ IYPHGS D LP+
Sbjct: 104 LHRYQTGRDSRVVTALTLLKKHLFSYQGHVSAALVLGGVDITGPHLHTIYPHGSTDTLPF 163
Query: 182 ATMGSGSLAAMAVFEAGWKRDM 247
ATMGSGSLAAM+VFEA +K +
Sbjct: 164 ATMGSGSLAAMSVFEAKYKEGL 185
Score = 63.3 bits (147), Expect = 1e-10
Identities = 35/71 (49%), Positives = 42/71 (59%)
Frame = +1
Query: 256 EGKKLVRDAIAAGIFNDLGSGSNVDLCVIRNTGPAQYLRTYEEANVKGKKQGSYRYALGT 435
EG KLV +AI +GIFNDLGSGSNVD+CVI G +YLR Y E N +
Sbjct: 189 EGIKLVAEAICSGIFNDLGSGSNVDICVI-TKGHKEYLRNYMEPNPRTYVSSKGYSFTKK 247
Query: 436 TAVLKQRVIPL 468
T VL ++ PL
Sbjct: 248 TEVLLTKITPL 258
>At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1)
identical to 20S proteasome beta subunit PBB1 (PBB1)
GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
677-692 (1998)); contains Pfam profile: PF00227
proteasome A-type and B-type;
Length = 273
Score = 118 bits (284), Expect = 3e-27
Identities = 56/82 (68%), Positives = 64/82 (78%)
Frame = +2
Query: 2 LQRLHTGRTVPVETAATLLKRMLFRYQGHIGAALVLGGVDRTGPHIYCIYPHGSVDKLPY 181
L R TGR V TA TLLK+ LF YQGH+ AALVLGGVD TGPH++ IYPHGS D LP+
Sbjct: 104 LHRYQTGRDSRVITALTLLKKHLFSYQGHVSAALVLGGVDITGPHLHTIYPHGSTDTLPF 163
Query: 182 ATMGSGSLAAMAVFEAGWKRDM 247
ATMGSGSLAAM+VFEA +K +
Sbjct: 164 ATMGSGSLAAMSVFEAKYKEGL 185
Score = 62.1 bits (144), Expect = 3e-10
Identities = 34/71 (47%), Positives = 42/71 (59%)
Frame = +1
Query: 256 EGKKLVRDAIAAGIFNDLGSGSNVDLCVIRNTGPAQYLRTYEEANVKGKKQGSYRYALGT 435
EG KLV ++I +GIFNDLGSGSNVD+CVI G +YLR Y E N +
Sbjct: 189 EGIKLVAESICSGIFNDLGSGSNVDICVI-TKGNKEYLRNYMEPNPRTYVSSKGYSFTKK 247
Query: 436 TAVLKQRVIPL 468
T VL ++ PL
Sbjct: 248 TEVLLTKITPL 258
>At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1)
identical to 20S proteasome beta subunit PBB1 (PBB1)
GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
677-692 (1998)); contains Pfam profile: PF00227
proteasome A-type and B-type;
Length = 267
Score = 118 bits (284), Expect = 3e-27
Identities = 56/82 (68%), Positives = 64/82 (78%)
Frame = +2
Query: 2 LQRLHTGRTVPVETAATLLKRMLFRYQGHIGAALVLGGVDRTGPHIYCIYPHGSVDKLPY 181
L R TGR V TA TLLK+ LF YQGH+ AALVLGGVD TGPH++ IYPHGS D LP+
Sbjct: 104 LHRYQTGRDSRVITALTLLKKHLFSYQGHVSAALVLGGVDITGPHLHTIYPHGSTDTLPF 163
Query: 182 ATMGSGSLAAMAVFEAGWKRDM 247
ATMGSGSLAAM+VFEA +K +
Sbjct: 164 ATMGSGSLAAMSVFEAKYKEGL 185
Score = 60.1 bits (139), Expect = 1e-09
Identities = 29/45 (64%), Positives = 34/45 (75%)
Frame = +1
Query: 256 EGKKLVRDAIAAGIFNDLGSGSNVDLCVIRNTGPAQYLRTYEEAN 390
EG KLV ++I +GIFNDLGSGSNVD+CVI G +YLR Y E N
Sbjct: 189 EGIKLVAESICSGIFNDLGSGSNVDICVI-TKGNKEYLRNYMEPN 232
>At1g13060.1 68414.m01514 20S proteasome beta subunit E1 (PBE1)
(PRCE) identical to GB:O23717; identical to cDNA
proteasome subunit prce GI:2511595
Length = 274
Score = 50.0 bits (114), Expect = 1e-06
Identities = 29/84 (34%), Positives = 44/84 (52%), Gaps = 1/84 (1%)
Frame = +2
Query: 2 LQRLHTGRTVPVETAATLLKRMLFRYQGH-IGAALVLGGVDRTGPHIYCIYPHGSVDKLP 178
L L R + V A+ LL ML+ Y+G + ++ G D TGP +Y + G K
Sbjct: 122 LHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWDETGPGLYYVDNEGGRLKGD 181
Query: 179 YATMGSGSLAAMAVFEAGWKRDMN 250
++GSGS A V ++G+K DM+
Sbjct: 182 RFSVGSGSPYAYGVLDSGYKYDMS 205
>At3g26340.1 68416.m03286 20S proteasome beta subunit E, putative
very strong similarity to SP|O23717 Proteasome subunit
beta type 5 precursor (EC 3.4.25.1) (20S proteasome
subunit E) (Proteasome epsilon chain) {Arabidopsis
thaliana}
Length = 273
Score = 49.6 bits (113), Expect = 1e-06
Identities = 29/84 (34%), Positives = 44/84 (52%), Gaps = 1/84 (1%)
Frame = +2
Query: 2 LQRLHTGRTVPVETAATLLKRMLFRYQGH-IGAALVLGGVDRTGPHIYCIYPHGSVDKLP 178
L L R + V A+ LL ML+ Y+G + ++ G D TGP +Y + G K
Sbjct: 122 LHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWDETGPGLYYVDNEGGRLKGD 181
Query: 179 YATMGSGSLAAMAVFEAGWKRDMN 250
++GSGS A V ++G+K DM+
Sbjct: 182 RFSVGSGSPYAYGVLDSGYKFDMS 205
>At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1)
(PRCD) identical to cDNA proteasome subunit prcd
GI:2511593
Length = 233
Score = 44.4 bits (100), Expect = 6e-05
Identities = 24/77 (31%), Positives = 41/77 (53%), Gaps = 1/77 (1%)
Frame = +2
Query: 20 GRTVPVETAATLLKRMLFRYQGHIGAALVLGGVDR-TGPHIYCIYPHGSVDKLPYATMGS 196
G+ V+ +A L++ + + + + L++GG D+ G IY I G+V + P+A GS
Sbjct: 83 GQPATVKVSANLIRMLAYNNKNMLQTGLIVGGWDKYEGGKIYGIPLGGTVVEQPFAIGGS 142
Query: 197 GSLAAMAVFEAGWKRDM 247
GS F+ WK +M
Sbjct: 143 GSSYLYGFFDQAWKDNM 159
>At2g27020.1 68415.m03244 20S proteasome alpha subunit G (PAG1)
(PRC8) identical to proteasome subunit alpha type 3
SP:O23715, GI:12644056 from [Arabidopsis thaliana];
identical to cDNA proteasome subunit prc8 GI:2511591
Length = 249
Score = 37.1 bits (82), Expect = 0.008
Identities = 18/45 (40%), Positives = 23/45 (51%)
Frame = +2
Query: 92 GAALVLGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMAVFE 226
G ++LGG DR GP +Y I P G + A +G G AA E
Sbjct: 133 GCGVILGGYDRDGPQLYMIEPSGISYRYFGAAIGKGKQAAKTEIE 177
>At5g42790.1 68418.m05212 20S proteasome alpha subunit F1 (PAF1)
(gb|AAC32062.1)
Length = 278
Score = 33.5 bits (73), Expect = 0.10
Identities = 16/45 (35%), Positives = 24/45 (53%)
Frame = +2
Query: 92 GAALVLGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMAVFE 226
G L++GG+D +G H+Y P G+ + +GS S AA E
Sbjct: 129 GVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLE 173
>At1g47250.1 68414.m05231 20S proteasome alpha subunit F2 (PAF2)
(PRC2B) (PRS1) identical to GB:AAC32063 from
[Arabidopsis thaliana] (Genetics 149 (2), 677-692
(1998)); identical to cDNA proteasome subunit prc2b
GI:2511585
Length = 277
Score = 33.5 bits (73), Expect = 0.10
Identities = 16/45 (35%), Positives = 24/45 (53%)
Frame = +2
Query: 92 GAALVLGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMAVFE 226
G L++GG+D +G H+Y P G+ + +GS S AA E
Sbjct: 129 GVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLE 173
>At1g53850.1 68414.m06129 20S proteasome alpha subunit E1 (PAE1)
identical to 20S proteasome subunit PAE1 GI:3421087 from
[Arabidopsis thaliana]
Length = 237
Score = 32.3 bits (70), Expect = 0.24
Identities = 15/53 (28%), Positives = 28/53 (52%)
Frame = +2
Query: 92 GAALVLGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMAVFEAGWKRDMN 250
G +L++ G D GP +Y P G+ + +GSGS A + + + +D++
Sbjct: 136 GVSLLIAGHDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQFNKDLS 188
>At1g79210.1 68414.m09235 20S proteasome alpha subunit B, putative
nearly identical to SP|O23708 Proteasome subunit alpha
type 2 (EC 3.4.25.1) (20S proteasome alpha subunit B)
{Arabidopsis thaliana} and to At1g16470
Length = 235
Score = 31.9 bits (69), Expect = 0.32
Identities = 16/52 (30%), Positives = 23/52 (44%)
Frame = +2
Query: 92 GAALVLGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMAVFEAGWKRDM 247
G +L++ G D GP +Y + P GS + MG A E + DM
Sbjct: 131 GVSLLVAGYDDKGPQLYQVDPSGSYFSWKASAMGKNVSNAKTFLEKRYTEDM 182
>At1g16470.1 68414.m01970 20S proteasome alpha subunit B (PAB1)
(PRC3) identical to proteasome subunit alpha type 2
SP:O23708, GI:6093778; identical to cDNA proteasome
subunit prc3 GI:2511573
Length = 235
Score = 31.9 bits (69), Expect = 0.32
Identities = 16/52 (30%), Positives = 23/52 (44%)
Frame = +2
Query: 92 GAALVLGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMAVFEAGWKRDM 247
G +L++ G D GP +Y + P GS + MG A E + DM
Sbjct: 131 GVSLLVAGYDDKGPQLYQVDPSGSYFSWKASAMGKNVSNAKTFLEKRYTEDM 182
>At3g14290.1 68416.m01808 20S proteasome alpha subunit E2 (PAE2)
identical to 20S proteasome subunit PAE2 GB:AAC32061
from [Arabidopsis thaliana]
Length = 237
Score = 31.5 bits (68), Expect = 0.42
Identities = 15/52 (28%), Positives = 27/52 (51%)
Frame = +2
Query: 92 GAALVLGGVDRTGPHIYCIYPHGSVDKLPYATMGSGSLAAMAVFEAGWKRDM 247
G +L++ G D GP +Y P G+ + +GSGS A + + + +D+
Sbjct: 136 GVSLLIAGHDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQFNKDI 187
>At1g79670.1 68414.m09291 wall-associated kinase, putative similar
to wall-associated kinase 4 GI:3355308 from [Arabidopsis
thaliana]; isoform contains non-consensus AT-acceptor
splice site.
Length = 751
Score = 31.5 bits (68), Expect = 0.42
Identities = 10/18 (55%), Positives = 14/18 (77%)
Frame = +3
Query: 390 CEGEKARLVQICSWYNCC 443
C+G+K+RL +IC Y CC
Sbjct: 166 CDGDKSRLDKICGGYTCC 183
>At3g51180.1 68416.m05603 zinc finger (CCCH-type) family protein
contains Pfam domain, PF00642: Zinc finger
C-x8-C-x5-C-x3-H type (and similar)
Length = 521
Score = 29.5 bits (63), Expect = 1.7
Identities = 29/112 (25%), Positives = 47/112 (41%), Gaps = 1/112 (0%)
Frame = -3
Query: 488 TKIDSTSRGITRCLSTAVV-PRAYLYEPCFFPFTLASS*VLRYWAGPVLRITHRSTLEPE 312
T I+S +R + + +S P A +P P + + V R + P R+ S +P
Sbjct: 257 TNINSATRPVPQPVSAVTASPMARKPQPVIIPQEHSVA-VSRSFTNPERRV---SPPKPV 312
Query: 311 PKSLNIPAAIASRTSFFPSPHSYHVSNLLQTLP*PQEIRIPWLHMVIYLQIH 156
S++ P I + S P P +HV + PQ R P + + L H
Sbjct: 313 NGSISPPKPITGKPSSIPMPVHFHVGIAKEQ---PQPARFPSSSLPMNLNFH 361
>At1g77850.1 68414.m09072 transcriptional factor B3 family protein
similar to auxin response factor 10 GI:6165644 from
[Arabidopsis thaliana]; contains Pfam profile PF02362:
B3 DNA binding domain
Length = 585
Score = 27.5 bits (58), Expect = 6.8
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = -3
Query: 305 SLNIPAAIASRTSFFPSPHSYHVSNLLQTLP 213
S+ IP + SR +FP H H LL TLP
Sbjct: 28 SVQIPV-LHSRVYYFPQGHVEHCCPLLSTLP 57
>At5g23170.1 68418.m02710 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 341
Score = 27.1 bits (57), Expect = 9.0
Identities = 12/28 (42%), Positives = 17/28 (60%)
Frame = +1
Query: 460 IPLEVESILVRPTEAFRLWMWSLKRVVK 543
I E+ + L P ++ LWM L+RVVK
Sbjct: 292 ITAEIVACLAEPLKSLPLWMSVLRRVVK 319
>At1g55480.1 68414.m06346 expressed protein
Length = 335
Score = 27.1 bits (57), Expect = 9.0
Identities = 14/32 (43%), Positives = 17/32 (53%)
Frame = +2
Query: 128 GPHIYCIYPHGSVDKLPYATMGSGSLAAMAVF 223
G +I I P GS DK T+G +A AVF
Sbjct: 108 GTYIDAILPGGSADKTGKFTVGDRVIATSAVF 139
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,515,224
Number of Sequences: 28952
Number of extensions: 259944
Number of successful extensions: 668
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 651
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 666
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1121903184
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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