Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00912
(709 letters)
Database: tribolium
336 sequences; 122,585 total letters
Searching.......................................................done
Score E
Sequences producing significant alignments: (bits) Value
DQ157471-1|AAZ85125.1| 1639|Tribolium castaneum Down Syndrome ad... 59 3e-11
DQ490059-1|ABF22614.1| 947|Tribolium castaneum short gastrulati... 23 1.8
AM292363-1|CAL23175.2| 347|Tribolium castaneum gustatory recept... 22 4.3
AY887136-1|AAW78361.1| 580|Tribolium castaneum vasa RNA helicas... 22 5.6
>DQ157471-1|AAZ85125.1| 1639|Tribolium castaneum Down Syndrome
adhesion molecule splicevariant 3.12.3.1 protein.
Length = 1639
Score = 59.3 bits (137), Expect = 3e-11
Identities = 26/84 (30%), Positives = 46/84 (54%)
Frame = +2
Query: 5 SAAPPVFVSTPQKRFAKINETSVMFDCTATGFPKPHVAWYFNGERMLLNERIILQHNGSI 184
SA+ P +++ F + V C A G P P + W G + +++I Q +GS+
Sbjct: 1302 SASVPAKIASFDDTFTTTYKEDVTLPCLAVGLPPPVITWKIKGVQFTTSDKIRQQPDGSL 1361
Query: 185 LIKNVQNSDAGVYTCQVENIHGKN 256
I++V ++AG Y+C VEN +G++
Sbjct: 1362 FIRDVSRNNAGEYSCHVENDYGQD 1385
Score = 42.3 bits (95), Expect = 4e-06
Identities = 23/85 (27%), Positives = 40/85 (47%), Gaps = 6/85 (7%)
Frame = +1
Query: 271 LEVTAPPSFIVVPTNQTAIIGDNVWFTCKAKGTPKPSIKWYRNTVILP------ITENIV 432
L + PP +I+ PT++ G + CKA G P+P + W R T + P N
Sbjct: 693 LFINVPPRWILEPTDKAFAQGSDAAVECKADGFPRPVVTWKRATGVSPGDYKDFKPNNPD 752
Query: 433 LSDDNQNLTLLEVTRDDDAIYHCRA 507
+ ++ LT+ + + ++ Y C A
Sbjct: 753 IKVEDGTLTINNIQKTNEGYYLCEA 777
Score = 41.1 bits (92), Expect = 9e-06
Identities = 29/92 (31%), Positives = 40/92 (43%), Gaps = 8/92 (8%)
Frame = +1
Query: 256 SASVPLEVTAPPSFIVVPTNQTAIIGDNVWFTCKAKGTPKPSIKWYRNTVIL-------- 411
SA + + V APP F + NQT+ GD C+AKG I W N L
Sbjct: 786 SAVIQISVQAPPQFDIKLRNQTSRRGDPAVLQCEAKGEKPIGILWNINNKRLEPKGDNRY 845
Query: 412 PITENIVLSDDNQNLTLLEVTRDDDAIYHCRA 507
I E I+ + L++ R D A++ C A
Sbjct: 846 TIREEILANGVLSGLSIKRTERSDSALFTCVA 877
Score = 40.3 bits (90), Expect = 2e-05
Identities = 22/77 (28%), Positives = 41/77 (53%), Gaps = 1/77 (1%)
Frame = +2
Query: 23 FVSTPQKRFAKINETSVMFDCTATGFPKPHVAWYFNGERMLLNERIILQHNGSILIKNVQ 202
FV + +K+ A + +++ C G P V W +G ++ +N + + NG+++I+NV+
Sbjct: 513 FVRSMEKQ-AIVAGGTLIVHCPFAGHPVDSVVWERDGRQLPINRKQKVFINGTLIIENVE 571
Query: 203 N-SDAGVYTCQVENIHG 250
SD Y C +N G
Sbjct: 572 RASDQATYDCVAKNSQG 588
Score = 39.1 bits (87), Expect = 3e-05
Identities = 22/74 (29%), Positives = 37/74 (50%), Gaps = 6/74 (8%)
Frame = +2
Query: 47 FAKINETSVMFDCTATGFPKPHVAWY------FNGERMLLNERIILQHNGSILIKNVQNS 208
+ + ++ +V C A GFP P WY + + LN+R + Q G+++I+ +
Sbjct: 242 YQRHSKQNVALLCPAQGFPSPSFRWYKFVEGTTRKQAVTLNDR-VKQVAGTLIIREAKVE 300
Query: 209 DAGVYTCQVENIHG 250
D+G Y C V N G
Sbjct: 301 DSGKYLCVVNNSVG 314
Score = 38.7 bits (86), Expect = 5e-05
Identities = 25/84 (29%), Positives = 42/84 (50%), Gaps = 8/84 (9%)
Frame = +2
Query: 14 PPVFVSTP-QKRFAKINETSVMFDCTATGFPKPHVAWYF-------NGERMLLNERIILQ 169
PP ++ P K FA+ ++ +V +C A GFP+P V W + + N I
Sbjct: 698 PPRWILEPTDKAFAQGSDAAV--ECKADGFPRPVVTWKRATGVSPGDYKDFKPNNPDIKV 755
Query: 170 HNGSILIKNVQNSDAGVYTCQVEN 241
+G++ I N+Q ++ G Y C+ N
Sbjct: 756 EDGTLTINNIQKTNEGYYLCEAVN 779
Score = 36.3 bits (80), Expect = 2e-04
Identities = 25/83 (30%), Positives = 36/83 (43%)
Frame = +1
Query: 253 KSASVPLEVTAPPSFIVVPTNQTAIIGDNVWFTCKAKGTPKPSIKWYRNTVILPITENIV 432
+S L VTAP + P QT G FTC +G P +I W ++ PI N
Sbjct: 316 ESVETVLTVTAPLKAKIEPQVQTIDFGRPATFTCNFEGNPIKTISWLKDG--HPIDHNEA 373
Query: 433 LSDDNQNLTLLEVTRDDDAIYHC 501
+ L + V ++D +Y C
Sbjct: 374 V------LRIESVRKEDKGMYQC 390
Score = 35.1 bits (77), Expect = 6e-04
Identities = 27/78 (34%), Positives = 37/78 (47%), Gaps = 13/78 (16%)
Frame = +2
Query: 35 PQKRFAKINET-----SVMFDCTATGFPKPHVAWYFNGERMLLNER-IILQH---NGSIL 187
PQ R A ET SV C A+G P P + W G R+ +ER I Q+ NG ++
Sbjct: 414 PQIRHAFNEETVQPGNSVFLKCIASGNPTPEITWELYGRRLSNSERNQIGQYVTVNGDVV 473
Query: 188 ----IKNVQNSDAGVYTC 229
I + +D G+Y C
Sbjct: 474 SHLNITAIHTNDGGLYRC 491
Score = 34.3 bits (75), Expect = 0.001
Identities = 14/35 (40%), Positives = 18/35 (51%)
Frame = +2
Query: 17 PVFVSTPQKRFAKINETSVMFDCTATGFPKPHVAW 121
PVFV P R N T + +C+A G P P + W
Sbjct: 23 PVFVREPPNRIDFSNTTGAVVECSAHGNPTPDIIW 57
Score = 31.9 bits (69), Expect = 0.005
Identities = 34/127 (26%), Positives = 52/127 (40%)
Frame = +1
Query: 130 RRKNVIKRAHNFTT*WIYSYQKCSKFRCGCLHMSS*KHTWKKSASVPLEVTAPPSFIVVP 309
R +N+ +A N + Y + + G S K T SASVP ++ SF
Sbjct: 1261 RNQNLSHQAKNLDSNLKYEFWVTAATTIGEGQPSK-KVTVSPSASVPAKIA---SF---D 1313
Query: 310 TNQTAIIGDNVWFTCKAKGTPKPSIKWYRNTVILPITENIVLSDDNQNLTLLEVTRDDDA 489
T ++V C A G P P I W V ++ I D +L + +V+R++
Sbjct: 1314 DTFTTTYKEDVTLPCLAVGLPPPVITWKIKGVQFTTSDKIRQQPDG-SLFIRDVSRNNAG 1372
Query: 490 IYHCRAE 510
Y C E
Sbjct: 1373 EYSCHVE 1379
Score = 29.5 bits (63), Expect = 0.028
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = +1
Query: 337 NVWFTCKAKGTPKPSIKWYR 396
NV C A+G P PS +WY+
Sbjct: 249 NVALLCPAQGFPSPSFRWYK 268
Score = 29.5 bits (63), Expect = 0.028
Identities = 20/81 (24%), Positives = 34/81 (41%), Gaps = 7/81 (8%)
Frame = +1
Query: 286 PPSFIVVPTNQTAIIGDNVWFTCKAKGTPKPSIKW------YRNTVILPITENIVLSDD- 444
PP +T G++V+ C A G P P I W N+ I + + ++ D
Sbjct: 413 PPQIRHAFNEETVQPGNSVFLKCIASGNPTPEITWELYGRRLSNSERNQIGQYVTVNGDV 472
Query: 445 NQNLTLLEVTRDDDAIYHCRA 507
+L + + +D +Y C A
Sbjct: 473 VSHLNITAIHTNDGGLYRCVA 493
Score = 29.1 bits (62), Expect = 0.037
Identities = 20/68 (29%), Positives = 31/68 (45%), Gaps = 6/68 (8%)
Frame = +2
Query: 56 INE-TSVMFDCTATGFPKP-HVAWYFNGERMLLNERIILQHNG----SILIKNVQNSDAG 217
INE V CT + P +++W NG + I++ ++ I NVQ++ G
Sbjct: 615 INEGDGVSVQCTVSKGDYPLNISWTLNGHFIDQTNGIVINRASKRVSTLSIDNVQSTHVG 674
Query: 218 VYTCQVEN 241
YTC N
Sbjct: 675 NYTCLASN 682
Score = 28.3 bits (60), Expect = 0.065
Identities = 9/38 (23%), Positives = 20/38 (52%)
Frame = +3
Query: 567 IPPRIVLKPEDMDAFKETSVQMPCEIESVPPASIDWRK 680
+PPR +L+P D + + + C+ + P + W++
Sbjct: 697 VPPRWILEPTDKAFAQGSDAAVECKADGFPRPVVTWKR 734
Score = 27.1 bits (57), Expect = 0.15
Identities = 13/60 (21%), Positives = 24/60 (40%)
Frame = +3
Query: 528 EASAVLTLEQVEIIPPRIVLKPEDMDAFKETSVQMPCEIESVPPASIDWRKDGSRIVEDD 707
+ S +T+ +P +I + + V +PC +PP I W+ G + D
Sbjct: 1292 QPSKKVTVSPSASVPAKIASFDDTFTTTYKEDVTLPCLAVGLPPPVITWKIKGVQFTTSD 1351
Score = 26.6 bits (56), Expect = 0.20
Identities = 21/73 (28%), Positives = 29/73 (39%), Gaps = 1/73 (1%)
Frame = +1
Query: 295 FIVVPTNQTAIIGDNVWFTCKAKGTPKPSIKWYRNTVILPITENIVLSDDNQNLTLLEVT 474
F+ Q + G + C G P S+ W R+ LPI + N L + V
Sbjct: 513 FVRSMEKQAIVAGGTLIVHCPFAGHPVDSVVWERDGRQLPINRKQKVF-INGTLIIENVE 571
Query: 475 R-DDDAIYHCRAE 510
R D A Y C A+
Sbjct: 572 RASDQATYDCVAK 584
Score = 25.0 bits (52), Expect = 0.60
Identities = 13/41 (31%), Positives = 17/41 (41%), Gaps = 1/41 (2%)
Frame = +1
Query: 280 TAPPSFIVVPTNQTAIIGDN-VWFTCKAKGTPKPSIKWYRN 399
T+ P F+ P N+ C A G P P I W R+
Sbjct: 20 TSGPVFVREPPNRIDFSNTTGAVVECSAHGNPTPDIIWVRS 60
Score = 22.6 bits (46), Expect = 3.2
Identities = 9/20 (45%), Positives = 10/20 (50%)
Frame = +3
Query: 636 CEIESVPPASIDWRKDGSRI 695
C E P +I W KDG I
Sbjct: 349 CNFEGNPIKTISWLKDGHPI 368
Score = 21.8 bits (44), Expect = 5.6
Identities = 9/42 (21%), Positives = 18/42 (42%)
Frame = +3
Query: 570 PPRIVLKPEDMDAFKETSVQMPCEIESVPPASIDWRKDGSRI 695
PP+ +K + + + + CE + P I W + R+
Sbjct: 796 PPQFDIKLRNQTSRRGDPAVLQCEAKGEKPIGILWNINNKRL 837
>DQ490059-1|ABF22614.1| 947|Tribolium castaneum short gastrulation
protein.
Length = 947
Score = 23.4 bits (48), Expect = 1.8
Identities = 17/42 (40%), Positives = 24/42 (57%), Gaps = 2/42 (4%)
Frame = -1
Query: 700 STILEPSFLQSIEAGGT--LSISQGICTEVSLNASISSGFKT 581
S++LEP+ AGGT +SIS + T V L A + +G T
Sbjct: 280 SSLLEPAVGTMTGAGGTAIVSISTSVSTSVYL-AIVFNGLFT 320
>AM292363-1|CAL23175.2| 347|Tribolium castaneum gustatory receptor
candidate 42 protein.
Length = 347
Score = 22.2 bits (45), Expect = 4.3
Identities = 7/19 (36%), Positives = 13/19 (68%)
Frame = -3
Query: 434 STIFSVIGNITVFLYHLIE 378
+T+ +IGN+T F +I+
Sbjct: 324 TTLLQIIGNVTTFFIIIIQ 342
>AY887136-1|AAW78361.1| 580|Tribolium castaneum vasa RNA helicase
protein.
Length = 580
Score = 21.8 bits (44), Expect = 5.6
Identities = 11/32 (34%), Positives = 14/32 (43%)
Frame = -3
Query: 350 VNQTLSPIIAV*FVGTTIKDGGAVTSNGTDAD 255
V + P +AV F+G D G G D D
Sbjct: 71 VTSGVIPGVAVDFLGENNDDSGGGRGGGRDGD 102
Database: tribolium
Posted date: Oct 23, 2007 1:18 PM
Number of letters in database: 122,585
Number of sequences in database: 336
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 178,933
Number of Sequences: 336
Number of extensions: 4147
Number of successful extensions: 25
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 23
length of database: 122,585
effective HSP length: 55
effective length of database: 104,105
effective search space used: 18738900
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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