Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00910
         (704 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef...   148   9e-37
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef...   148   9e-37
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef...   148   9e-37
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote...    46   5e-06
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E...    40   3e-04
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa...    38   0.001
SPAC23D3.08 |usp108||U1 snRNP-associated protein Usp108|Schizosa...    28   1.1  
SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr 1|||Ma...    28   1.1  
SPCC417.10 |||membrane transporter|Schizosaccharomyces pombe|chr...    28   1.5  
SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomy...    27   2.0  
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni...    27   3.5  
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc...    26   6.0  
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos...    25   8.0  

>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
           Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 460

 Score =  148 bits (358), Expect = 9e-37
 Identities = 66/85 (77%), Positives = 74/85 (87%)
 Frame = +1

Query: 256 ETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAG 435
           ETGV+KPG IV FAPA +TTEVKSVEMHHE+L   +PGDNVGFNVKNVSVK++RRG V G
Sbjct: 266 ETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCG 325

Query: 436 DSKNNPPKGAADFTAQVIVLNHPGQ 510
           DSKN+PP G A FTAQVI+LNHPGQ
Sbjct: 326 DSKNDPPMGCASFTAQVIILNHPGQ 350



 Score =  145 bits (351), Expect = 6e-36
 Identities = 62/86 (72%), Positives = 73/86 (84%)
 Frame = +2

Query: 2   YNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPARPT 181
           +NP  V FVP+SG+ GDNM+EP+T MPW++GWQ E K G   GK L+EA+D+I PPARPT
Sbjct: 181 FNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPT 240

Query: 182 DKPLRLPLQDVYKIGGIGTVPVGRVK 259
           DKPLRLPLQDVYKIGGIGTVPVGRV+
Sbjct: 241 DKPLRLPLQDVYKIGGIGTVPVGRVE 266



 Score =  108 bits (260), Expect = 7e-25
 Identities = 50/92 (54%), Positives = 62/92 (67%)
 Frame = +3

Query: 429 CW*LQKQPT*GCCRFYSSSHCA*PSWAISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGK 608
           C   +  P  GC  F +          IS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK
Sbjct: 324 CGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGK 383

Query: 609 STEVNPKSIKSGDAAIVNLVPSKPLCVESFQE 704
             E +PK +KSGDA I  +VPSKP+CVE+F +
Sbjct: 384 KIEESPKFVKSGDACIAKMVPSKPMCVEAFTD 415


>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
           Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 460

 Score =  148 bits (358), Expect = 9e-37
 Identities = 66/85 (77%), Positives = 74/85 (87%)
 Frame = +1

Query: 256 ETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAG 435
           ETGV+KPG IV FAPA +TTEVKSVEMHHE+L   +PGDNVGFNVKNVSVK++RRG V G
Sbjct: 266 ETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCG 325

Query: 436 DSKNNPPKGAADFTAQVIVLNHPGQ 510
           DSKN+PP G A FTAQVI+LNHPGQ
Sbjct: 326 DSKNDPPMGCASFTAQVIILNHPGQ 350



 Score =  145 bits (351), Expect = 6e-36
 Identities = 62/86 (72%), Positives = 73/86 (84%)
 Frame = +2

Query: 2   YNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPARPT 181
           +NP  V FVP+SG+ GDNM+EP+T MPW++GWQ E K G   GK L+EA+D+I PPARPT
Sbjct: 181 FNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPT 240

Query: 182 DKPLRLPLQDVYKIGGIGTVPVGRVK 259
           DKPLRLPLQDVYKIGGIGTVPVGRV+
Sbjct: 241 DKPLRLPLQDVYKIGGIGTVPVGRVE 266



 Score =  108 bits (260), Expect = 7e-25
 Identities = 50/92 (54%), Positives = 62/92 (67%)
 Frame = +3

Query: 429 CW*LQKQPT*GCCRFYSSSHCA*PSWAISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGK 608
           C   +  P  GC  F +          IS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK
Sbjct: 324 CGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGK 383

Query: 609 STEVNPKSIKSGDAAIVNLVPSKPLCVESFQE 704
             E +PK +KSGDA I  +VPSKP+CVE+F +
Sbjct: 384 KIEESPKFVKSGDACIAKMVPSKPMCVEAFTD 415


>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
           Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 460

 Score =  148 bits (358), Expect = 9e-37
 Identities = 66/85 (77%), Positives = 74/85 (87%)
 Frame = +1

Query: 256 ETGVLKPGTIVVFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAG 435
           ETGV+KPG IV FAPA +TTEVKSVEMHHE+L   +PGDNVGFNVKNVSVK++RRG V G
Sbjct: 266 ETGVIKPGMIVTFAPAGVTTEVKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCG 325

Query: 436 DSKNNPPKGAADFTAQVIVLNHPGQ 510
           DSKN+PP G A FTAQVI+LNHPGQ
Sbjct: 326 DSKNDPPMGCASFTAQVIILNHPGQ 350



 Score =  145 bits (351), Expect = 6e-36
 Identities = 62/86 (72%), Positives = 73/86 (84%)
 Frame = +2

Query: 2   YNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPARPT 181
           +NP  V FVP+SG+ GDNM+EP+T MPW++GWQ E K G   GK L+EA+D+I PPARPT
Sbjct: 181 FNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPT 240

Query: 182 DKPLRLPLQDVYKIGGIGTVPVGRVK 259
           DKPLRLPLQDVYKIGGIGTVPVGRV+
Sbjct: 241 DKPLRLPLQDVYKIGGIGTVPVGRVE 266



 Score =  108 bits (260), Expect = 7e-25
 Identities = 50/92 (54%), Positives = 62/92 (67%)
 Frame = +3

Query: 429 CW*LQKQPT*GCCRFYSSSHCA*PSWAISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGK 608
           C   +  P  GC  F +          IS GY+PVLDCHTAHIACKFAE+ EK+DRR+GK
Sbjct: 324 CGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLDCHTAHIACKFAELIEKIDRRSGK 383

Query: 609 STEVNPKSIKSGDAAIVNLVPSKPLCVESFQE 704
             E +PK +KSGDA I  +VPSKP+CVE+F +
Sbjct: 384 KIEESPKFVKSGDACIAKMVPSKPMCVEAFTD 415


>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 592

 Score = 46.0 bits (104), Expect = 5e-06
 Identities = 31/88 (35%), Positives = 45/88 (51%), Gaps = 2/88 (2%)
 Frame = +2

Query: 2   YNPAAVAFVPISGWHGDNMLEPSTK--MPWFKGWQVERKEGKADGKCLIEALDAILPPAR 175
           +  + V FVPIS   G N+++  +     W+KG              L+ ALD ++PP +
Sbjct: 352 FKTSNVHFVPISAISGTNLIQKDSSDLYKWYKG------------PTLLSALDQLVPPEK 399

Query: 176 PTDKPLRLPLQDVYKIGGIGTVPVGRVK 259
           P  KPLRL + DVY+     TV  GRV+
Sbjct: 400 PYRKPLRLSIDDVYRSPRSVTV-TGRVE 426


>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
           EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 439

 Score = 39.9 bits (89), Expect = 3e-04
 Identities = 19/42 (45%), Positives = 28/42 (66%), Gaps = 1/42 (2%)
 Frame = +2

Query: 137 LIEALDAILP-PARPTDKPLRLPLQDVYKIGGIGTVPVGRVK 259
           L+EA+D+ +  P R TD P  + ++DV+ I G GTV  GRV+
Sbjct: 234 LMEAVDSYITLPERKTDVPFLMAIEDVFSISGRGTVVTGRVE 275



 Score = 38.3 bits (85), Expect = 0.001
 Identities = 20/60 (33%), Positives = 34/60 (56%), Gaps = 2/60 (3%)
 Frame = +1

Query: 256 ETGVLKPGTIV--VFAPANITTEVKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYV 429
           E G LK G  +  V   +++ T V  +EM  + L  AV GDN G  ++++  ++L+RG +
Sbjct: 275 ERGTLKKGAEIEIVGYGSHLKTTVTGIEMFKKQLDAAVAGDNCGLLLRSIKREQLKRGMI 334


>SPCC584.04 |sup35|erf3|translation release factor eRF3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 662

 Score = 38.3 bits (85), Expect = 0.001
 Identities = 19/65 (29%), Positives = 34/65 (52%)
 Frame = +3

Query: 510 ISNGYTPVLDCHTAHIACKFAEIKEKVDRRTGKSTEVNPKSIKSGDAAIVNLVPSKPLCV 689
           ++ GY+ V+  HTA     FA++  K+D +T + ++  P     G   I  L    P+C+
Sbjct: 573 LTTGYSCVMHIHTAVEEVSFAKLLHKLD-KTNRKSKKPPMFATKGMKIIAELETQTPVCM 631

Query: 690 ESFQE 704
           E F++
Sbjct: 632 ERFED 636


>SPAC23D3.08 |usp108||U1 snRNP-associated protein
           Usp108|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 382

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 26/77 (33%), Positives = 36/77 (46%), Gaps = 4/77 (5%)
 Frame = -3

Query: 417 TQFLDGHVLYVET---YIVSRYSFLESFVVHLHRFDFSSDVGGGKDNNGTWFQH-TSFTL 250
           T  +D  V+ +ET   YI+SR SF+    V  +  D +  + G   +  T F H +S   
Sbjct: 162 TNEIDASVV-IETLLKYILSRISFIPRVSVVPYIPDAAYGIYGSP-STFTQFNHMSSMVT 219

Query: 249 PTGTVPIPPILYTSCRG 199
           P     IPPI  TS  G
Sbjct: 220 PENPYGIPPIALTSAGG 236


>SPAC1952.11c |ure2||urease |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 835

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 15/46 (32%), Positives = 19/46 (41%)
 Frame = -3

Query: 177 GRAGGRMASRASMRHFPSALPSLRSTCHPLNQGILVEGSNMLSPCH 40
           G  GG      S+   P+ LPS  +   P     L E  +ML  CH
Sbjct: 545 GAGGGHAPDIISLVQNPNILPSSTNPTRPFTTNTLDEELDMLMVCH 590


>SPCC417.10 |||membrane transporter|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 508

 Score = 27.9 bits (59), Expect = 1.5
 Identities = 17/57 (29%), Positives = 26/57 (45%), Gaps = 2/57 (3%)
 Frame = -1

Query: 326 DLTSVVMLAGAKTTMVP--GFNTPVSLCRRARYQYHRFCIRLAGEDAGACQWGGQVA 162
           D+ + +M   +    +P  G  T  SL  +    Y      L G  AGAC++GG +A
Sbjct: 289 DIRTWIMFVSSVLLNIPNGGIGTFSSLLIKGTMGYDTLQTLLMGLPAGACEFGGLIA 345


>SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1115

 Score = 27.5 bits (58), Expect = 2.0
 Identities = 11/34 (32%), Positives = 19/34 (55%)
 Frame = -3

Query: 330 HRFDFSSDVGGGKDNNGTWFQHTSFTLPTGTVPI 229
           H++DF  D    K ++G+   H + T PT + P+
Sbjct: 59  HKYDFEIDRDSLKSDSGSPRLHQNATAPTSSTPL 92


>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
           Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 2199

 Score = 26.6 bits (56), Expect = 3.5
 Identities = 12/25 (48%), Positives = 15/25 (60%)
 Frame = -3

Query: 414 QFLDGHVLYVETYIVSRYSFLESFV 340
           +F DGH+L  ETY+      LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551


>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
            |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1233

 Score = 25.8 bits (54), Expect = 6.0
 Identities = 10/39 (25%), Positives = 22/39 (56%)
 Frame = -3

Query: 438  VTSNITTTQFLDGHVLYVETYIVSRYSFLESFVVHLHRF 322
            +T ++ +   L  H+  +++   SR +FL   ++H+H F
Sbjct: 1012 LTQSLQSFSHLSNHIEVLDSTRQSRLTFLCHLILHMHGF 1050


>SPAC631.01c |acp2||F-actin capping protein beta subunit
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 268

 Score = 25.4 bits (53), Expect = 8.0
 Identities = 11/25 (44%), Positives = 18/25 (72%)
 Frame = -2

Query: 655 MAASPDLMDFGLTSVDLPVRRSTFS 581
           ++ +PDL D  L+SVD P++ +T S
Sbjct: 27  LSVAPDLADVLLSSVDQPLKVNTCS 51


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,331,469
Number of Sequences: 5004
Number of extensions: 74468
Number of successful extensions: 226
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 207
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 226
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 327172622
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)