Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00908
         (659 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|ch...   111   7e-26
SPBC19G7.06 |mbx1||MADS-box transcription factor Mbx1|Schizosacc...    28   1.0  
SPAC13G7.04c |mac1||membrane anchored protein Mac1 |Schizosaccha...    27   1.8  
SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces...    27   1.8  
SPCC1919.02 |||pig-X|Schizosaccharomyces pombe|chr 3|||Manual          27   2.4  
SPBC8D2.15 |||mitochondrial lipoic acid synthetase |Schizosaccha...    27   3.2  
SPAC11D3.07c |||transcription factor|Schizosaccharomyces pombe|c...    27   3.2  
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit...    26   4.2  
SPBC17F3.01c |rga5|SPBC557.01|GTPase activating protein Rga5|Sch...    26   4.2  
SPBC25B2.07c |mug164||microtubule-associated protein|Schizosacch...    26   4.2  
SPAC16C9.02c |||S-methyl-5-thioadenosine phosphorylase|Schizosac...    26   5.5  
SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces...    26   5.5  
SPAC328.04 |||AAA family ATPase, unknown biological role|Schizos...    25   7.3  
SPBC1A4.07c |||U3 snoRNP-associated protein Sof1|Schizosaccharom...    25   7.3  
SPAC22G7.04 |ubp13|pan2|poly|Schizosaccharomyces pombe|chr 1|||M...    25   7.3  
SPAP27G11.10c |nup184||nucleoporin Nup184|Schizosaccharomyces po...    25   9.7  
SPCC736.14 |dis1||microtubule-associated protein Dis1 |Schizosac...    25   9.7  

>SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 509

 Score =  111 bits (268), Expect = 7e-26
 Identities = 54/82 (65%), Positives = 64/82 (78%)
 Frame = +2

Query: 260 LGSRKGVNLPGIPVDLPAVSEKDKSDLLFGVEQGVDMIFASFIRNGAALHEIRGILGEKG 439
           + S+KGVNLP   VDLPA+SEKDK+DL FGV+ GVDMIFASFIR    +  IR +LGE+G
Sbjct: 180 ISSKKGVNLPKTDVDLPALSEKDKADLRFGVKNGVDMIFASFIRRAEDVIHIREVLGEEG 239

Query: 440 KNIKIISKIENHQGMVNLDEII 505
           KNIKII KIEN QG+ N D I+
Sbjct: 240 KNIKIICKIENQQGVNNFDSIL 261



 Score = 82.6 bits (195), Expect = 5e-17
 Identities = 38/48 (79%), Positives = 42/48 (87%)
 Frame = +1

Query: 511 SDGIMVARGDLGIEIPPEKVFLAQKTMIARCNRVGKPVICATQMLESM 654
           +DGIMVARGDLGIEIP  +VF+AQK MIA+CN  GKPV CATQMLESM
Sbjct: 264 TDGIMVARGDLGIEIPASQVFVAQKMMIAKCNIAGKPVACATQMLESM 311



 Score = 69.7 bits (163), Expect = 3e-13
 Identities = 34/84 (40%), Positives = 48/84 (57%), Gaps = 1/84 (1%)
 Frame = +3

Query: 3   PEIRTGLLEGGGSAEVELKKGETIKLTTSSDYQEKGNADTIYVDYKNITNVVKPGNRIFI 182
           PEIRTGL  GG   +  +  G  +  TT   Y EK N   +Y+DYKNIT V++PG  I++
Sbjct: 95  PEIRTGLTVGG--TDYPISSGHEMIFTTDDAYAEKCNDKVMYIDYKNITKVIQPGRIIYV 152

Query: 183 DDGLISI-ICQSVSADTLTCTIEN 251
           DDG++S  + + V    L   + N
Sbjct: 153 DDGILSFTVIEKVDDKNLKVRVNN 176


>SPBC19G7.06 |mbx1||MADS-box transcription factor
           Mbx1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 436

 Score = 28.3 bits (60), Expect = 1.0
 Identities = 13/23 (56%), Positives = 15/23 (65%)
 Frame = +3

Query: 297 PWTYPQSPRRTSRTCSSASNKAL 365
           P T P SPRR+ +  S A NKAL
Sbjct: 7   PSTAPSSPRRSIQRISDAKNKAL 29


>SPAC13G7.04c |mac1||membrane anchored protein Mac1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 756

 Score = 27.5 bits (58), Expect = 1.8
 Identities = 28/113 (24%), Positives = 41/113 (36%)
 Frame = +3

Query: 111 NADTIYVDYKNITNVVKPGNRIFIDDGLISIICQSVSADTLTCTIENGVCSDPGKASTCP 290
           + DT+   Y +    VK    + +DD L S+       D LT T E G  SD G      
Sbjct: 185 HVDTMNSSYSSYKTDVK--YPLALDDKLSSVPTLPKFHDALTSTSEFGPPSDSGDTVGTT 242

Query: 291 AYPWTYPQSPRRTSRTCSSASNKALI*FSRHSSATAPRCTRFAASSAKRGRTS 449
            YP     +    S   S   ++     SR + +       F  S +   R+S
Sbjct: 243 QYPGDIKYATGYESTVASPVPSRVAKLSSRDTPSIIADYDEFRKSESSPSRSS 295


>SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 713

 Score = 27.5 bits (58), Expect = 1.8
 Identities = 13/33 (39%), Positives = 18/33 (54%)
 Frame = -1

Query: 245 NGTRKSVSADRLTDDRDEAIIDEDSVSWLHDIR 147
           N TRKS+S  + TD      + +D  SWL  +R
Sbjct: 576 NKTRKSISHSKNTDKHVGNELPQDIPSWLRSLR 608


>SPCC1919.02 |||pig-X|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 332

 Score = 27.1 bits (57), Expect = 2.4
 Identities = 15/56 (26%), Positives = 23/56 (41%)
 Frame = -3

Query: 657 NHGFQHLSRADHWFSNPIASGDHGLLGEEYLFWRDLDTQISASNHDTIGLAIISSK 490
           N    HLSR ++W   P     H  +  E      +D    A N++ +  A +S K
Sbjct: 54  NINISHLSRENYWVETPFGKVFHSEVVSEEFMQIFIDKGNKALNNEEMIFACMSEK 109


>SPBC8D2.15 |||mitochondrial lipoic acid synthetase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 370

 Score = 26.6 bits (56), Expect = 3.2
 Identities = 10/24 (41%), Positives = 14/24 (58%)
 Frame = +2

Query: 119 YNLRGLQEHNECREARKPNLHR*W 190
           ++LRG   H  C EA+ PN+   W
Sbjct: 89  HDLRGSHLHTVCEEAKCPNIGECW 112


>SPAC11D3.07c |||transcription factor|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 603

 Score = 26.6 bits (56), Expect = 3.2
 Identities = 14/33 (42%), Positives = 18/33 (54%)
 Frame = +2

Query: 329 KSDLLFGVEQGVDMIFASFIRNGAALHEIRGIL 427
           K +LLFGV+ G D+IF   +       EIR  L
Sbjct: 341 KYELLFGVQSGGDLIFVPTVSLTIIQSEIRNRL 373


>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
            hand and WH2 motif |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1794

 Score = 26.2 bits (55), Expect = 4.2
 Identities = 18/54 (33%), Positives = 22/54 (40%)
 Frame = -3

Query: 435  FSPRMPRISCSAAPLRMNDAKIISTPCSTPKSRSDLSFSETAGRSTGMPGRLTP 274
            FSPRM  ++ S +PL MN A   S       S     F+  A      P  L P
Sbjct: 1588 FSPRMGPVNNSGSPLAMNAAGQPSLAVPAVPSAPSNHFNPFAKMQPPAPSPLQP 1641


>SPBC17F3.01c |rga5|SPBC557.01|GTPase activating protein
           Rga5|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 361

 Score = 26.2 bits (55), Expect = 4.2
 Identities = 18/56 (32%), Positives = 26/56 (46%)
 Frame = +3

Query: 372 FSRHSSATAPRCTRFAASSAKRGRTSRSSPRLKITREWSI*TRL*LIRWYHGCSRR 539
           FSRHSSAT         S+ K       SP    +R +S+ +R   ++ +H   RR
Sbjct: 302 FSRHSSATYSNSPSSNFSNMKSSEVDPGSPPRIKSRSYSL-SRSSSMKLFHTLDRR 356


>SPBC25B2.07c |mug164||microtubule-associated
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 501

 Score = 26.2 bits (55), Expect = 4.2
 Identities = 22/74 (29%), Positives = 31/74 (41%)
 Frame = +3

Query: 3   PEIRTGLLEGGGSAEVELKKGETIKLTTSSDYQEKGNADTIYVDYKNITNVVKPGNRIFI 182
           P  RT          V    G   K +TS   + K NA T  V   +    V+PG+RIF 
Sbjct: 239 PPTRTSTTRPLSRVNVTNASGSISKNSTSPS-KVKVNASTKIVRPVSAAQTVRPGSRIFR 297

Query: 183 DDGLISIICQSVSA 224
           ++   +    +VSA
Sbjct: 298 ENSASNTQRPNVSA 311


>SPAC16C9.02c |||S-methyl-5-thioadenosine
           phosphorylase|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 307

 Score = 25.8 bits (54), Expect = 5.5
 Identities = 11/22 (50%), Positives = 12/22 (54%)
 Frame = +3

Query: 279 STCPAYPWTYPQSPRRTSRTCS 344
           S  P  PW YP SP   +RT S
Sbjct: 29  SVNPITPWGYPASPISIARTTS 50


>SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 513

 Score = 25.8 bits (54), Expect = 5.5
 Identities = 10/17 (58%), Positives = 11/17 (64%)
 Frame = -2

Query: 67  SPFFSSTSAEPPPSKSP 17
           SP FSS+S  PPP   P
Sbjct: 231 SPIFSSSSTPPPPPPRP 247


>SPAC328.04 |||AAA family ATPase, unknown biological
           role|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 741

 Score = 25.4 bits (53), Expect = 7.3
 Identities = 21/59 (35%), Positives = 26/59 (44%)
 Frame = +3

Query: 162 PGNRIFIDDGLISIICQSVSADTLTCTIENGVCSDPGKASTCPAYPWTYPQSPRRTSRT 338
           PG+R FIDDG  S    S      T  +++ V +    AS  P  P T   S    SRT
Sbjct: 121 PGDRSFIDDGNYS--ASSFERQNRTAPLQSKVTT----ASLKPNIPRTSMSSSPTASRT 173


>SPBC1A4.07c |||U3 snoRNP-associated protein
           Sof1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 436

 Score = 25.4 bits (53), Expect = 7.3
 Identities = 11/30 (36%), Positives = 16/30 (53%)
 Frame = +1

Query: 244 LKTEYARIPERRQPARHTRGPTRSLREGQV 333
           L+  Y  IPE R+ ARH   PT   +  ++
Sbjct: 368 LRERYKHIPEIRRIARHRHLPTNVKKAAEI 397


>SPAC22G7.04 |ubp13|pan2|poly|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1115

 Score = 25.4 bits (53), Expect = 7.3
 Identities = 12/32 (37%), Positives = 19/32 (59%)
 Frame = -3

Query: 582  LGEEYLFWRDLDTQISASNHDTIGLAIISSKL 487
            L  ++L W  LD +I  + HD+I  A+ + KL
Sbjct: 1028 LSLKFLAWYLLDEEIQLTEHDSIEDALTALKL 1059


>SPAP27G11.10c |nup184||nucleoporin Nup184|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1564

 Score = 25.0 bits (52), Expect = 9.7
 Identities = 13/45 (28%), Positives = 23/45 (51%)
 Frame = -2

Query: 262 EHTPFSMVHVRVSALTD*QMIEMRPSSMKIRFPGFTTFVMFL*ST 128
           ++ PF+++H  +S+L D   I+     M   F    ++V F  ST
Sbjct: 377 KYDPFTLIHRIISSLEDDPYIDGYSKIMATLFSSAVSYVKFSDST 421


>SPCC736.14 |dis1||microtubule-associated protein Dis1
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 882

 Score = 25.0 bits (52), Expect = 9.7
 Identities = 19/68 (27%), Positives = 33/68 (48%), Gaps = 6/68 (8%)
 Frame = -3

Query: 438 PFSPRMPRISCSAAPLRMNDAKIISTP------CSTPKSRSDLSFSETAGRSTGMPGRLT 277
           P  PR+PR+   A+PL+ +  K+  TP       S+  S++ L+    + RS+      T
Sbjct: 541 PSRPRLPRV---ASPLKTSPVKLAVTPQAPSPLPSSNPSQASLTEESLSTRSSPTKPSTT 597

Query: 276 PFRDPSIL 253
             R  S++
Sbjct: 598 SLRSQSLV 605


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,718,339
Number of Sequences: 5004
Number of extensions: 56219
Number of successful extensions: 204
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 193
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 203
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 299817502
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)