BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00894
(739 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos... 96 5e-21
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 48 1e-06
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 46 7e-06
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 44 2e-05
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 43 4e-05
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 43 5e-05
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 39 6e-04
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 37 0.003
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 36 0.006
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 36 0.006
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 36 0.008
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 34 0.018
SPBC17D1.01 ||SPBC17D11.09|sequence orphan|Schizosaccharomyces p... 33 0.056
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 32 0.098
SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit Pst... 29 0.69
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 29 0.69
SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki... 28 1.6
SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 26 4.9
SPBC16E9.01c |php4|SPBP16F5.09c|CCAAT-binding factor complex sub... 26 6.4
>SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme
Mms2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 95.9 bits (228), Expect = 5e-21
Identities = 40/93 (43%), Positives = 62/93 (66%)
Frame = +1
Query: 232 KGSGRWNHSWGLESDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFI 411
KG G + S+GL + DD+TL+ W I+GP + +ENR+YSLKI C YPD PP F+
Sbjct: 19 KGLGESSCSYGLTNADDITLSDWNATILGPAHSVHENRIYSLKIHCDANYPDAPPIVTFV 78
Query: 412 SRIHMNCVNSQTGLVDNRQVPILARWQRDYTIK 510
SRI++ V+ +TG V+ ++ L W+R+Y+++
Sbjct: 79 SRINLPGVDGETGKVNPHKIDCLRHWKREYSME 111
Score = 39.1 bits (87), Expect = 6e-04
Identities = 15/22 (68%), Positives = 21/22 (95%)
Frame = +2
Query: 188 VPRNFRLLEELEQGQKGVGDGT 253
VPRNF+LLEELE+G+KG+G+ +
Sbjct: 4 VPRNFKLLEELEKGEKGLGESS 25
Score = 35.5 bits (78), Expect = 0.008
Identities = 15/28 (53%), Positives = 20/28 (71%)
Frame = +3
Query: 507 QTVLQEVRRLMTLKDNMKLSQPPEGSTF 590
+TVL ++++ M N KL QPPEGSTF
Sbjct: 111 ETVLLDLKKEMASSSNRKLPQPPEGSTF 138
>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 217
Score = 48.0 bits (109), Expect = 1e-06
Identities = 28/75 (37%), Positives = 40/75 (53%), Gaps = 1/75 (1%)
Frame = +1
Query: 289 LTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIHMNCVNSQTGLVDNRQ 468
++H GM GP TPYE + + IE YP PP F ++I+ V+SQTG +
Sbjct: 33 ISHLKGMFRGPEGTPYEGGYFVVDIEIPIDYPFRPPKMNFDTKIYHPNVSSQTGAI---C 89
Query: 469 VPILA-RWQRDYTIK 510
+ IL +W YT+K
Sbjct: 90 LDILKDQWSPVYTMK 104
>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 147
Score = 45.6 bits (103), Expect = 7e-06
Identities = 20/51 (39%), Positives = 28/51 (54%)
Frame = +1
Query: 289 LTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIHMNCVNS 441
L HW I+GP +PY ++ L I T YP +PP F +RI+ +NS
Sbjct: 30 LFHWQATIMGPADSPYAGGVFFLSIHFPTDYPFKPPKVNFTTRIYHPNINS 80
>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 176
Score = 44.0 bits (99), Expect = 2e-05
Identities = 19/47 (40%), Positives = 22/47 (46%)
Frame = +1
Query: 274 DDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFIS 414
D D L HW G I GP T YE + + + YP PPT F S
Sbjct: 53 DSDSNLLHWAGTITGPSDTYYEGLKFKISMSFPANYPYSPPTIIFTS 99
>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 151
Score = 43.2 bits (97), Expect = 4e-05
Identities = 17/45 (37%), Positives = 28/45 (62%), Gaps = 1/45 (2%)
Frame = +1
Query: 298 WTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRI-HMN 429
W +IIGP TP+E+ + L + +YP++PP +F+S + H N
Sbjct: 36 WNAVIIGPADTPFEDGTFKLVLSFDEQYPNKPPLVKFVSTMFHPN 80
>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 160
Score = 42.7 bits (96), Expect = 5e-05
Identities = 20/51 (39%), Positives = 27/51 (52%), Gaps = 1/51 (1%)
Frame = +1
Query: 280 DMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRI-HMN 429
D+ + HW +I GP TPYE + L I YP PP+ F ++I H N
Sbjct: 31 DVDMFHWKAVIEGPTETPYEGGQWVLDIHVHEGYPISPPSVYFQTKIVHPN 81
>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
Hus5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 157
Score = 39.1 bits (87), Expect = 6e-04
Identities = 21/60 (35%), Positives = 28/60 (46%), Gaps = 1/60 (1%)
Frame = +1
Query: 271 SDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRI-HMNCVNSQT 447
SD + L +W I G P+T +E +Y L + YP PP RF + H N S T
Sbjct: 32 SDGGLDLMNWKVGIPGKPKTSWEGGLYKLTMAFPEEYPTRPPKCRFTPPLFHPNVYPSGT 91
>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
Length = 167
Score = 37.1 bits (82), Expect = 0.003
Identities = 19/52 (36%), Positives = 25/52 (48%), Gaps = 1/52 (1%)
Frame = +1
Query: 277 DDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRI-HMN 429
DD ++ W MIIGP T YE + + YP PP +F + I H N
Sbjct: 31 DDKSIFEWEVMIIGPEDTLYEGGFFHATLSFPQDYPLMPPKMKFTTEIWHPN 82
>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 35.9 bits (79), Expect = 0.006
Identities = 16/42 (38%), Positives = 21/42 (50%)
Frame = +1
Query: 289 LTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFIS 414
+ W +I GPP TPYE Y + YP +PP R I+
Sbjct: 34 ILEWHYIITGPPDTPYEGGQYHGTLIFPPDYPFKPPAIRMIT 75
>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
Length = 155
Score = 35.9 bits (79), Expect = 0.006
Identities = 15/48 (31%), Positives = 23/48 (47%)
Frame = +1
Query: 280 DMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIH 423
D L HW +GP + Y + ++ YP +PPT F +RI+
Sbjct: 33 DDNLFHWACTALGPSDSVYAGGKFHFSLKFPLDYPFQPPTIEFTTRIY 80
>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 166
Score = 35.5 bits (78), Expect = 0.008
Identities = 17/46 (36%), Positives = 26/46 (56%)
Frame = +1
Query: 271 SDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARF 408
S++D T W +I GP TP+E +Y ++ + YP PPT +F
Sbjct: 30 SNEDDFFT-WDCLIQGPDGTPFEGGLYPATLKFPSDYPLGPPTLKF 74
>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 34.3 bits (75), Expect = 0.018
Identities = 14/47 (29%), Positives = 26/47 (55%)
Frame = +1
Query: 316 GPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIHMNCVNSQTGLV 456
GP TPY ++ + +E + YP + P+ F++RI ++ +G V
Sbjct: 38 GPSETPYSGGIWKVHVELPSEYPWKSPSIGFVNRIFHPNIDELSGSV 84
>SPBC17D1.01 ||SPBC17D11.09|sequence orphan|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 584
Score = 32.7 bits (71), Expect = 0.056
Identities = 18/38 (47%), Positives = 23/38 (60%), Gaps = 1/38 (2%)
Frame = -2
Query: 306 SSPMSKSHIIIAFKTPRMVPSPTPFWPCSSS-SKRRKF 196
SSPMS+SH+ I+ + PR S P P S + K RKF
Sbjct: 439 SSPMSESHVQISSEMPRTGMSALPMRPRSQNVDKHRKF 476
>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
Length = 148
Score = 31.9 bits (69), Expect = 0.098
Identities = 12/36 (33%), Positives = 21/36 (58%)
Frame = +1
Query: 316 GPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIH 423
GP ++ YE + L++ YP PP RF+++I+
Sbjct: 40 GPQQSAYEGGKFHLELFLPDEYPMMPPNVRFLTKIY 75
>SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit
Pst2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1075
Score = 29.1 bits (62), Expect = 0.69
Identities = 17/47 (36%), Positives = 27/47 (57%), Gaps = 4/47 (8%)
Frame = +1
Query: 238 SGRWNHSWGLESDDDMTLTHW----TGMIIGPPRTPYENRMYSLKIE 366
SGR + +WG+ +DD ++ W +G I+ +TPYE M L+ E
Sbjct: 354 SGRDDFAWGILNDDWVSHPTWASEESGFIV-QRKTPYEEAMTKLEEE 399
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 29.1 bits (62), Expect = 0.69
Identities = 14/32 (43%), Positives = 20/32 (62%)
Frame = -3
Query: 128 KLRLIQSSFIA*ALPKQIRFI*SKYGNLQQSN 33
K RL++S F +LP Q+RF+ Y NL+ N
Sbjct: 27 KFRLLESQFALYSLPCQLRFV--HYNNLEDPN 56
>SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase
kinase Win1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1436
Score = 27.9 bits (59), Expect = 1.6
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = -2
Query: 486 SSKNWDLSIIHKSSLTIDTVHMNPRNKAGCWW 391
S+ NW S+++ SL D + NP+NK W
Sbjct: 291 STVNWVQSVLNLPSLLSDDLMANPKNKERFEW 322
>SPCC777.10c |ubc12||ubiquitin conjugating enzyme
Ubc12|Schizosaccharomyces pombe|chr 3|||Manual
Length = 177
Score = 26.2 bits (55), Expect = 4.9
Identities = 9/37 (24%), Positives = 19/37 (51%)
Frame = +1
Query: 313 IGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIH 423
I P Y+ + +I+ YP +PP + +++I+
Sbjct: 58 IRPDEGYYKGGKFKFRIQIDDNYPHDPPKVKCLNKIY 94
>SPBC16E9.01c |php4|SPBP16F5.09c|CCAAT-binding factor complex
subunit Php4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 295
Score = 25.8 bits (54), Expect = 6.4
Identities = 14/35 (40%), Positives = 20/35 (57%)
Frame = -2
Query: 315 NNHSSPMSKSHIIIAFKTPRMVPSPTPFWPCSSSS 211
++ S+PM K+ IAFK P M P+ P +S S
Sbjct: 258 SSKSAPMLKAGPSIAFKLPTMEPNFNDVRPVTSIS 292
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,010,892
Number of Sequences: 5004
Number of extensions: 61933
Number of successful extensions: 148
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 143
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 148
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 349251756
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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