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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00894
         (739 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos...    96   5e-21
SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch...    48   1e-06
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha...    46   7e-06
SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2...    44   2e-05
SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz...    43   4e-05
SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac...    43   5e-05
SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch...    39   6e-04
SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac...    37   0.003
SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc...    36   0.006
SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac...    36   0.006
SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo...    36   0.008
SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch...    34   0.018
SPBC17D1.01 ||SPBC17D11.09|sequence orphan|Schizosaccharomyces p...    33   0.056
SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch...    32   0.098
SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit Pst...    29   0.69 
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo...    29   0.69 
SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki...    28   1.6  
SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac...    26   4.9  
SPBC16E9.01c |php4|SPBP16F5.09c|CCAAT-binding factor complex sub...    26   6.4  

>SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme
           Mms2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 139

 Score = 95.9 bits (228), Expect = 5e-21
 Identities = 40/93 (43%), Positives = 62/93 (66%)
 Frame = +1

Query: 232 KGSGRWNHSWGLESDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFI 411
           KG G  + S+GL + DD+TL+ W   I+GP  + +ENR+YSLKI C   YPD PP   F+
Sbjct: 19  KGLGESSCSYGLTNADDITLSDWNATILGPAHSVHENRIYSLKIHCDANYPDAPPIVTFV 78

Query: 412 SRIHMNCVNSQTGLVDNRQVPILARWQRDYTIK 510
           SRI++  V+ +TG V+  ++  L  W+R+Y+++
Sbjct: 79  SRINLPGVDGETGKVNPHKIDCLRHWKREYSME 111



 Score = 39.1 bits (87), Expect = 6e-04
 Identities = 15/22 (68%), Positives = 21/22 (95%)
 Frame = +2

Query: 188 VPRNFRLLEELEQGQKGVGDGT 253
           VPRNF+LLEELE+G+KG+G+ +
Sbjct: 4   VPRNFKLLEELEKGEKGLGESS 25



 Score = 35.5 bits (78), Expect = 0.008
 Identities = 15/28 (53%), Positives = 20/28 (71%)
 Frame = +3

Query: 507 QTVLQEVRRLMTLKDNMKLSQPPEGSTF 590
           +TVL ++++ M    N KL QPPEGSTF
Sbjct: 111 ETVLLDLKKEMASSSNRKLPQPPEGSTF 138


>SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme
           Ubc1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 217

 Score = 48.0 bits (109), Expect = 1e-06
 Identities = 28/75 (37%), Positives = 40/75 (53%), Gaps = 1/75 (1%)
 Frame = +1

Query: 289 LTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIHMNCVNSQTGLVDNRQ 468
           ++H  GM  GP  TPYE   + + IE    YP  PP   F ++I+   V+SQTG +    
Sbjct: 33  ISHLKGMFRGPEGTPYEGGYFVVDIEIPIDYPFRPPKMNFDTKIYHPNVSSQTGAI---C 89

Query: 469 VPILA-RWQRDYTIK 510
           + IL  +W   YT+K
Sbjct: 90  LDILKDQWSPVYTMK 104


>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
           Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 147

 Score = 45.6 bits (103), Expect = 7e-06
 Identities = 20/51 (39%), Positives = 28/51 (54%)
 Frame = +1

Query: 289 LTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIHMNCVNS 441
           L HW   I+GP  +PY   ++ L I   T YP +PP   F +RI+   +NS
Sbjct: 30  LFHWQATIMGPADSPYAGGVFFLSIHFPTDYPFKPPKVNFTTRIYHPNINS 80


>SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 176

 Score = 44.0 bits (99), Expect = 2e-05
 Identities = 19/47 (40%), Positives = 22/47 (46%)
 Frame = +1

Query: 274 DDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFIS 414
           D D  L HW G I GP  T YE   + + +     YP  PPT  F S
Sbjct: 53  DSDSNLLHWAGTITGPSDTYYEGLKFKISMSFPANYPYSPPTIIFTS 99


>SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme
           E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 151

 Score = 43.2 bits (97), Expect = 4e-05
 Identities = 17/45 (37%), Positives = 28/45 (62%), Gaps = 1/45 (2%)
 Frame = +1

Query: 298 WTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRI-HMN 429
           W  +IIGP  TP+E+  + L +    +YP++PP  +F+S + H N
Sbjct: 36  WNAVIIGPADTPFEDGTFKLVLSFDEQYPNKPPLVKFVSTMFHPN 80


>SPBC1198.09 |ubc16||ubiquitin conjugating enzyme
           Ubc16|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 160

 Score = 42.7 bits (96), Expect = 5e-05
 Identities = 20/51 (39%), Positives = 27/51 (52%), Gaps = 1/51 (1%)
 Frame = +1

Query: 280 DMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRI-HMN 429
           D+ + HW  +I GP  TPYE   + L I     YP  PP+  F ++I H N
Sbjct: 31  DVDMFHWKAVIEGPTETPYEGGQWVLDIHVHEGYPISPPSVYFQTKIVHPN 81


>SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme
           Hus5|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 157

 Score = 39.1 bits (87), Expect = 6e-04
 Identities = 21/60 (35%), Positives = 28/60 (46%), Gaps = 1/60 (1%)
 Frame = +1

Query: 271 SDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRI-HMNCVNSQT 447
           SD  + L +W   I G P+T +E  +Y L +     YP  PP  RF   + H N   S T
Sbjct: 32  SDGGLDLMNWKVGIPGKPKTSWEGGLYKLTMAFPEEYPTRPPKCRFTPPLFHPNVYPSGT 91


>SPBC1105.09 |ubc15||ubiquitin conjugating enzyme
           Ubc15|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 167

 Score = 37.1 bits (82), Expect = 0.003
 Identities = 19/52 (36%), Positives = 25/52 (48%), Gaps = 1/52 (1%)
 Frame = +1

Query: 277 DDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRI-HMN 429
           DD ++  W  MIIGP  T YE   +   +     YP  PP  +F + I H N
Sbjct: 31  DDKSIFEWEVMIIGPEDTLYEGGFFHATLSFPQDYPLMPPKMKFTTEIWHPN 82


>SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme
           Ubc6|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 227

 Score = 35.9 bits (79), Expect = 0.006
 Identities = 16/42 (38%), Positives = 21/42 (50%)
 Frame = +1

Query: 289 LTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFIS 414
           +  W  +I GPP TPYE   Y   +     YP +PP  R I+
Sbjct: 34  ILEWHYIITGPPDTPYEGGQYHGTLIFPPDYPFKPPAIRMIT 75


>SPAC1250.03 |ubc14||ubiquitin conjugating enzyme
           Ubc14|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 155

 Score = 35.9 bits (79), Expect = 0.006
 Identities = 15/48 (31%), Positives = 23/48 (47%)
 Frame = +1

Query: 280 DMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIH 423
           D  L HW    +GP  + Y    +   ++    YP +PPT  F +RI+
Sbjct: 33  DDNLFHWACTALGPSDSVYAGGKFHFSLKFPLDYPFQPPTIEFTTRIY 80


>SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme
           Ubc3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 166

 Score = 35.5 bits (78), Expect = 0.008
 Identities = 17/46 (36%), Positives = 26/46 (56%)
 Frame = +1

Query: 271 SDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARF 408
           S++D   T W  +I GP  TP+E  +Y   ++  + YP  PPT +F
Sbjct: 30  SNEDDFFT-WDCLIQGPDGTPFEGGLYPATLKFPSDYPLGPPTLKF 74


>SPBC211.07c |ubc8||ubiquitin conjugating enzyme
           Ubc8|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 184

 Score = 34.3 bits (75), Expect = 0.018
 Identities = 14/47 (29%), Positives = 26/47 (55%)
 Frame = +1

Query: 316 GPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIHMNCVNSQTGLV 456
           GP  TPY   ++ + +E  + YP + P+  F++RI    ++  +G V
Sbjct: 38  GPSETPYSGGIWKVHVELPSEYPWKSPSIGFVNRIFHPNIDELSGSV 84


>SPBC17D1.01 ||SPBC17D11.09|sequence orphan|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 584

 Score = 32.7 bits (71), Expect = 0.056
 Identities = 18/38 (47%), Positives = 23/38 (60%), Gaps = 1/38 (2%)
 Frame = -2

Query: 306 SSPMSKSHIIIAFKTPRMVPSPTPFWPCSSS-SKRRKF 196
           SSPMS+SH+ I+ + PR   S  P  P S +  K RKF
Sbjct: 439 SSPMSESHVQISSEMPRTGMSALPMRPRSQNVDKHRKF 476


>SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme
           Ubc13|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 148

 Score = 31.9 bits (69), Expect = 0.098
 Identities = 12/36 (33%), Positives = 21/36 (58%)
 Frame = +1

Query: 316 GPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIH 423
           GP ++ YE   + L++     YP  PP  RF+++I+
Sbjct: 40  GPQQSAYEGGKFHLELFLPDEYPMMPPNVRFLTKIY 75


>SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit
           Pst2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1075

 Score = 29.1 bits (62), Expect = 0.69
 Identities = 17/47 (36%), Positives = 27/47 (57%), Gaps = 4/47 (8%)
 Frame = +1

Query: 238 SGRWNHSWGLESDDDMTLTHW----TGMIIGPPRTPYENRMYSLKIE 366
           SGR + +WG+ +DD ++   W    +G I+   +TPYE  M  L+ E
Sbjct: 354 SGRDDFAWGILNDDWVSHPTWASEESGFIV-QRKTPYEEAMTKLEEE 399


>SPBC215.01 ||SPBC3B9.20|GTPase activating
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 834

 Score = 29.1 bits (62), Expect = 0.69
 Identities = 14/32 (43%), Positives = 20/32 (62%)
 Frame = -3

Query: 128 KLRLIQSSFIA*ALPKQIRFI*SKYGNLQQSN 33
           K RL++S F   +LP Q+RF+   Y NL+  N
Sbjct: 27  KFRLLESQFALYSLPCQLRFV--HYNNLEDPN 56


>SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase
           kinase Win1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1436

 Score = 27.9 bits (59), Expect = 1.6
 Identities = 12/32 (37%), Positives = 18/32 (56%)
 Frame = -2

Query: 486 SSKNWDLSIIHKSSLTIDTVHMNPRNKAGCWW 391
           S+ NW  S+++  SL  D +  NP+NK    W
Sbjct: 291 STVNWVQSVLNLPSLLSDDLMANPKNKERFEW 322


>SPCC777.10c |ubc12||ubiquitin conjugating enzyme
           Ubc12|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 177

 Score = 26.2 bits (55), Expect = 4.9
 Identities = 9/37 (24%), Positives = 19/37 (51%)
 Frame = +1

Query: 313 IGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIH 423
           I P    Y+   +  +I+    YP +PP  + +++I+
Sbjct: 58  IRPDEGYYKGGKFKFRIQIDDNYPHDPPKVKCLNKIY 94


>SPBC16E9.01c |php4|SPBP16F5.09c|CCAAT-binding factor complex
           subunit Php4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 295

 Score = 25.8 bits (54), Expect = 6.4
 Identities = 14/35 (40%), Positives = 20/35 (57%)
 Frame = -2

Query: 315 NNHSSPMSKSHIIIAFKTPRMVPSPTPFWPCSSSS 211
           ++ S+PM K+   IAFK P M P+     P +S S
Sbjct: 258 SSKSAPMLKAGPSIAFKLPTMEPNFNDVRPVTSIS 292


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,010,892
Number of Sequences: 5004
Number of extensions: 61933
Number of successful extensions: 148
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 143
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 148
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 349251756
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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