BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00894 (739 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos... 96 5e-21 SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosacch... 48 1e-06 SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 46 7e-06 SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2... 44 2e-05 SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enz... 43 4e-05 SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosac... 43 5e-05 SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosacch... 39 6e-04 SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosac... 37 0.003 SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosacc... 36 0.006 SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosac... 36 0.006 SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizo... 36 0.008 SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosacch... 34 0.018 SPBC17D1.01 ||SPBC17D11.09|sequence orphan|Schizosaccharomyces p... 33 0.056 SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Sch... 32 0.098 SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit Pst... 29 0.69 SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 29 0.69 SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki... 28 1.6 SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosac... 26 4.9 SPBC16E9.01c |php4|SPBP16F5.09c|CCAAT-binding factor complex sub... 26 6.4 >SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizosaccharomyces pombe|chr 3|||Manual Length = 139 Score = 95.9 bits (228), Expect = 5e-21 Identities = 40/93 (43%), Positives = 62/93 (66%) Frame = +1 Query: 232 KGSGRWNHSWGLESDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFI 411 KG G + S+GL + DD+TL+ W I+GP + +ENR+YSLKI C YPD PP F+ Sbjct: 19 KGLGESSCSYGLTNADDITLSDWNATILGPAHSVHENRIYSLKIHCDANYPDAPPIVTFV 78 Query: 412 SRIHMNCVNSQTGLVDNRQVPILARWQRDYTIK 510 SRI++ V+ +TG V+ ++ L W+R+Y+++ Sbjct: 79 SRINLPGVDGETGKVNPHKIDCLRHWKREYSME 111 Score = 39.1 bits (87), Expect = 6e-04 Identities = 15/22 (68%), Positives = 21/22 (95%) Frame = +2 Query: 188 VPRNFRLLEELEQGQKGVGDGT 253 VPRNF+LLEELE+G+KG+G+ + Sbjct: 4 VPRNFKLLEELEKGEKGLGESS 25 Score = 35.5 bits (78), Expect = 0.008 Identities = 15/28 (53%), Positives = 20/28 (71%) Frame = +3 Query: 507 QTVLQEVRRLMTLKDNMKLSQPPEGSTF 590 +TVL ++++ M N KL QPPEGSTF Sbjct: 111 ETVLLDLKKEMASSSNRKLPQPPEGSTF 138 >SPBC2D10.20 |ubc1||ubiquitin conjugating enzyme Ubc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 217 Score = 48.0 bits (109), Expect = 1e-06 Identities = 28/75 (37%), Positives = 40/75 (53%), Gaps = 1/75 (1%) Frame = +1 Query: 289 LTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIHMNCVNSQTGLVDNRQ 468 ++H GM GP TPYE + + IE YP PP F ++I+ V+SQTG + Sbjct: 33 ISHLKGMFRGPEGTPYEGGYFVVDIEIPIDYPFRPPKMNFDTKIYHPNVSSQTGAI---C 89 Query: 469 VPILA-RWQRDYTIK 510 + IL +W YT+K Sbjct: 90 LDILKDQWSPVYTMK 104 >SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccharomyces pombe|chr 2|||Manual Length = 147 Score = 45.6 bits (103), Expect = 7e-06 Identities = 20/51 (39%), Positives = 28/51 (54%) Frame = +1 Query: 289 LTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIHMNCVNS 441 L HW I+GP +PY ++ L I T YP +PP F +RI+ +NS Sbjct: 30 LFHWQATIMGPADSPYAGGVFFLSIHFPTDYPFKPPKVNFTTRIYHPNINS 80 >SPCC1259.15c |ubc11|ubcdp, ubcp4|ubiquitin conjugating enzyme E2-C |Schizosaccharomyces pombe|chr 3|||Manual Length = 176 Score = 44.0 bits (99), Expect = 2e-05 Identities = 19/47 (40%), Positives = 22/47 (46%) Frame = +1 Query: 274 DDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFIS 414 D D L HW G I GP T YE + + + YP PPT F S Sbjct: 53 DSDSNLLHWAGTITGPSDTYYEGLKFKISMSFPANYPYSPPTIIFTS 99 >SPAC18B11.07c |rhp6|ubc2|Rad6 homolog, ubiquitin conjugating enzyme E2 Rhp6|Schizosaccharomyces pombe|chr 1|||Manual Length = 151 Score = 43.2 bits (97), Expect = 4e-05 Identities = 17/45 (37%), Positives = 28/45 (62%), Gaps = 1/45 (2%) Frame = +1 Query: 298 WTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRI-HMN 429 W +IIGP TP+E+ + L + +YP++PP +F+S + H N Sbjct: 36 WNAVIIGPADTPFEDGTFKLVLSFDEQYPNKPPLVKFVSTMFHPN 80 >SPBC1198.09 |ubc16||ubiquitin conjugating enzyme Ubc16|Schizosaccharomyces pombe|chr 2|||Manual Length = 160 Score = 42.7 bits (96), Expect = 5e-05 Identities = 20/51 (39%), Positives = 27/51 (52%), Gaps = 1/51 (1%) Frame = +1 Query: 280 DMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRI-HMN 429 D+ + HW +I GP TPYE + L I YP PP+ F ++I H N Sbjct: 31 DVDMFHWKAVIEGPTETPYEGGQWVLDIHVHEGYPISPPSVYFQTKIVHPN 81 >SPAC30D11.13 |hus5|ubc9|SUMO conjugating enzyme Hus5|Schizosaccharomyces pombe|chr 1|||Manual Length = 157 Score = 39.1 bits (87), Expect = 6e-04 Identities = 21/60 (35%), Positives = 28/60 (46%), Gaps = 1/60 (1%) Frame = +1 Query: 271 SDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRI-HMNCVNSQT 447 SD + L +W I G P+T +E +Y L + YP PP RF + H N S T Sbjct: 32 SDGGLDLMNWKVGIPGKPKTSWEGGLYKLTMAFPEEYPTRPPKCRFTPPLFHPNVYPSGT 91 >SPBC1105.09 |ubc15||ubiquitin conjugating enzyme Ubc15|Schizosaccharomyces pombe|chr 2|||Manual Length = 167 Score = 37.1 bits (82), Expect = 0.003 Identities = 19/52 (36%), Positives = 25/52 (48%), Gaps = 1/52 (1%) Frame = +1 Query: 277 DDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRI-HMN 429 DD ++ W MIIGP T YE + + YP PP +F + I H N Sbjct: 31 DDKSIFEWEVMIIGPEDTLYEGGFFHATLSFPQDYPLMPPKMKFTTEIWHPN 82 >SPAC10F6.05c |ubc6||ubiquitin conjugating enzyme Ubc6|Schizosaccharomyces pombe|chr 1|||Manual Length = 227 Score = 35.9 bits (79), Expect = 0.006 Identities = 16/42 (38%), Positives = 21/42 (50%) Frame = +1 Query: 289 LTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFIS 414 + W +I GPP TPYE Y + YP +PP R I+ Sbjct: 34 ILEWHYIITGPPDTPYEGGQYHGTLIFPPDYPFKPPAIRMIT 75 >SPAC1250.03 |ubc14||ubiquitin conjugating enzyme Ubc14|Schizosaccharomyces pombe|chr 1|||Manual Length = 155 Score = 35.9 bits (79), Expect = 0.006 Identities = 15/48 (31%), Positives = 23/48 (47%) Frame = +1 Query: 280 DMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIH 423 D L HW +GP + Y + ++ YP +PPT F +RI+ Sbjct: 33 DDNLFHWACTALGPSDSVYAGGKFHFSLKFPLDYPFQPPTIEFTTRIY 80 >SPBP16F5.04 |ubc3|ubcp3|ubiquitin conjugating enzyme Ubc3|Schizosaccharomyces pombe|chr 2|||Manual Length = 166 Score = 35.5 bits (78), Expect = 0.008 Identities = 17/46 (36%), Positives = 26/46 (56%) Frame = +1 Query: 271 SDDDMTLTHWTGMIIGPPRTPYENRMYSLKIECGTRYPDEPPTARF 408 S++D T W +I GP TP+E +Y ++ + YP PPT +F Sbjct: 30 SNEDDFFT-WDCLIQGPDGTPFEGGLYPATLKFPSDYPLGPPTLKF 74 >SPBC211.07c |ubc8||ubiquitin conjugating enzyme Ubc8|Schizosaccharomyces pombe|chr 2|||Manual Length = 184 Score = 34.3 bits (75), Expect = 0.018 Identities = 14/47 (29%), Positives = 26/47 (55%) Frame = +1 Query: 316 GPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIHMNCVNSQTGLV 456 GP TPY ++ + +E + YP + P+ F++RI ++ +G V Sbjct: 38 GPSETPYSGGIWKVHVELPSEYPWKSPSIGFVNRIFHPNIDELSGSV 84 >SPBC17D1.01 ||SPBC17D11.09|sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 584 Score = 32.7 bits (71), Expect = 0.056 Identities = 18/38 (47%), Positives = 23/38 (60%), Gaps = 1/38 (2%) Frame = -2 Query: 306 SSPMSKSHIIIAFKTPRMVPSPTPFWPCSSS-SKRRKF 196 SSPMS+SH+ I+ + PR S P P S + K RKF Sbjct: 439 SSPMSESHVQISSEMPRTGMSALPMRPRSQNVDKHRKF 476 >SPAC11E3.04c |ubc13|spu13|ubiquitin conjugating enzyme Ubc13|Schizosaccharomyces pombe|chr 1|||Manual Length = 148 Score = 31.9 bits (69), Expect = 0.098 Identities = 12/36 (33%), Positives = 21/36 (58%) Frame = +1 Query: 316 GPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIH 423 GP ++ YE + L++ YP PP RF+++I+ Sbjct: 40 GPQQSAYEGGKFHLELFLPDEYPMMPPNVRFLTKIY 75 >SPAC23C11.15 |pst2||Clr6 histone deacetylase complex subunit Pst2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1075 Score = 29.1 bits (62), Expect = 0.69 Identities = 17/47 (36%), Positives = 27/47 (57%), Gaps = 4/47 (8%) Frame = +1 Query: 238 SGRWNHSWGLESDDDMTLTHW----TGMIIGPPRTPYENRMYSLKIE 366 SGR + +WG+ +DD ++ W +G I+ +TPYE M L+ E Sbjct: 354 SGRDDFAWGILNDDWVSHPTWASEESGFIV-QRKTPYEEAMTKLEEE 399 >SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 834 Score = 29.1 bits (62), Expect = 0.69 Identities = 14/32 (43%), Positives = 20/32 (62%) Frame = -3 Query: 128 KLRLIQSSFIA*ALPKQIRFI*SKYGNLQQSN 33 K RL++S F +LP Q+RF+ Y NL+ N Sbjct: 27 KFRLLESQFALYSLPCQLRFV--HYNNLEDPN 56 >SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase kinase Win1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1436 Score = 27.9 bits (59), Expect = 1.6 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = -2 Query: 486 SSKNWDLSIIHKSSLTIDTVHMNPRNKAGCWW 391 S+ NW S+++ SL D + NP+NK W Sbjct: 291 STVNWVQSVLNLPSLLSDDLMANPKNKERFEW 322 >SPCC777.10c |ubc12||ubiquitin conjugating enzyme Ubc12|Schizosaccharomyces pombe|chr 3|||Manual Length = 177 Score = 26.2 bits (55), Expect = 4.9 Identities = 9/37 (24%), Positives = 19/37 (51%) Frame = +1 Query: 313 IGPPRTPYENRMYSLKIECGTRYPDEPPTARFISRIH 423 I P Y+ + +I+ YP +PP + +++I+ Sbjct: 58 IRPDEGYYKGGKFKFRIQIDDNYPHDPPKVKCLNKIY 94 >SPBC16E9.01c |php4|SPBP16F5.09c|CCAAT-binding factor complex subunit Php4|Schizosaccharomyces pombe|chr 2|||Manual Length = 295 Score = 25.8 bits (54), Expect = 6.4 Identities = 14/35 (40%), Positives = 20/35 (57%) Frame = -2 Query: 315 NNHSSPMSKSHIIIAFKTPRMVPSPTPFWPCSSSS 211 ++ S+PM K+ IAFK P M P+ P +S S Sbjct: 258 SSKSAPMLKAGPSIAFKLPTMEPNFNDVRPVTSIS 292 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,010,892 Number of Sequences: 5004 Number of extensions: 61933 Number of successful extensions: 148 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 143 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 148 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 349251756 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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