BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00880 (774 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g59990.2 68416.m06698 methionyl aminopeptidase, putative / me... 137 7e-33 At3g59990.1 68416.m06697 methionyl aminopeptidase, putative / me... 137 7e-33 At2g44180.1 68415.m05496 methionyl aminopeptidase, putative / me... 134 6e-32 At3g51800.2 68416.m05681 metallopeptidase M24 family protein sim... 42 6e-04 At3g51800.1 68416.m05680 metallopeptidase M24 family protein sim... 42 6e-04 At4g30710.2 68417.m04353 expressed protein contains Pfam domain,... 30 2.0 At4g30710.1 68417.m04352 expressed protein contains Pfam domain,... 30 2.0 At1g04160.1 68414.m00406 myosin family protein contains Pfam pro... 28 7.9 >At3g59990.2 68416.m06698 methionyl aminopeptidase, putative / methionine aminopeptidase, putative / peptidase M, putative similar to Methionine aminopeptidase 2 (EC 3.4.11.18) from {Rattus norvegicus} SP|P38062, {Homo sapiens} SP|P50579; contains Pfam profile PF00557: metallopeptidase family M24; supporting cDNA gi|11344921|gb|AF300880.1|AF300880 Length = 439 Score = 137 bits (332), Expect = 7e-33 Identities = 60/84 (71%), Positives = 75/84 (89%) Frame = +3 Query: 6 FNPRYDPLVKGVQEATEAGIKASGVDVRLCDVGAAVQEVMESHEVELDGQMYQVKPIRNL 185 FNP +DPL+ +EAT GIK +G+DVRLCD+GAA+QEVMES+EVE++G+++QVK IRNL Sbjct: 230 FNPMFDPLLAASREATYTGIKEAGIDVRLCDIGAAIQEVMESYEVEINGKVFQVKSIRNL 289 Query: 186 NGHSIEPYKIHAGKTVPIVKGGEQ 257 NGHSI PY+IHAGK+VPIVKGGEQ Sbjct: 290 NGHSIGPYQIHAGKSVPIVKGGEQ 313 Score = 134 bits (324), Expect = 6e-32 Identities = 61/101 (60%), Positives = 75/101 (74%) Frame = +2 Query: 257 TRMEENEIYAIETFGSTGRGQVHDDMDCSHYMKNFDQQFVPLRLQSSKQLLNVINKNFGT 436 T+MEE E YAIETFGSTG+G V +D++CSHYMKNFD VPLRL +KQLL INKNF T Sbjct: 314 TKMEEGEFYAIETFGSTGKGYVREDLECSHYMKNFDAGHVPLRLPRAKQLLATINKNFST 373 Query: 437 LAFCKRWLERAGASRYAMALKDLCTRGWWTPTRPSATSRGA 559 LAFC+R+L+R G ++Y MALK+LC G P P +G+ Sbjct: 374 LAFCRRYLDRIGETKYLMALKNLCDSGIVQPYPPLCDVKGS 414 Score = 62.9 bits (146), Expect = 2e-10 Identities = 24/32 (75%), Positives = 28/32 (87%) Frame = +1 Query: 514 GVVDAYPPLCDIKGCYTAQFEHTILLRPTCKK 609 G+V YPPLCD+KG Y +QFEHTILLRPTCK+ Sbjct: 400 GIVQPYPPLCDVKGSYVSQFEHTILLRPTCKE 431 >At3g59990.1 68416.m06697 methionyl aminopeptidase, putative / methionine aminopeptidase, putative / peptidase M, putative similar to Methionine aminopeptidase 2 (EC 3.4.11.18) from {Rattus norvegicus} SP|P38062, {Homo sapiens} SP|P50579; contains Pfam profile PF00557: metallopeptidase family M24; supporting cDNA gi|11344921|gb|AF300880.1|AF300880 Length = 439 Score = 137 bits (332), Expect = 7e-33 Identities = 60/84 (71%), Positives = 75/84 (89%) Frame = +3 Query: 6 FNPRYDPLVKGVQEATEAGIKASGVDVRLCDVGAAVQEVMESHEVELDGQMYQVKPIRNL 185 FNP +DPL+ +EAT GIK +G+DVRLCD+GAA+QEVMES+EVE++G+++QVK IRNL Sbjct: 230 FNPMFDPLLAASREATYTGIKEAGIDVRLCDIGAAIQEVMESYEVEINGKVFQVKSIRNL 289 Query: 186 NGHSIEPYKIHAGKTVPIVKGGEQ 257 NGHSI PY+IHAGK+VPIVKGGEQ Sbjct: 290 NGHSIGPYQIHAGKSVPIVKGGEQ 313 Score = 134 bits (324), Expect = 6e-32 Identities = 61/101 (60%), Positives = 75/101 (74%) Frame = +2 Query: 257 TRMEENEIYAIETFGSTGRGQVHDDMDCSHYMKNFDQQFVPLRLQSSKQLLNVINKNFGT 436 T+MEE E YAIETFGSTG+G V +D++CSHYMKNFD VPLRL +KQLL INKNF T Sbjct: 314 TKMEEGEFYAIETFGSTGKGYVREDLECSHYMKNFDAGHVPLRLPRAKQLLATINKNFST 373 Query: 437 LAFCKRWLERAGASRYAMALKDLCTRGWWTPTRPSATSRGA 559 LAFC+R+L+R G ++Y MALK+LC G P P +G+ Sbjct: 374 LAFCRRYLDRIGETKYLMALKNLCDSGIVQPYPPLCDVKGS 414 Score = 62.9 bits (146), Expect = 2e-10 Identities = 24/32 (75%), Positives = 28/32 (87%) Frame = +1 Query: 514 GVVDAYPPLCDIKGCYTAQFEHTILLRPTCKK 609 G+V YPPLCD+KG Y +QFEHTILLRPTCK+ Sbjct: 400 GIVQPYPPLCDVKGSYVSQFEHTILLRPTCKE 431 >At2g44180.1 68415.m05496 methionyl aminopeptidase, putative / methionine aminopeptidase, putative / peptidase M, putative similar to SP|P50579 Methionine aminopeptidase 2 (EC 3.4.11.18) (MetAP 2) {Homo sapiens}; contains Pfam profile PF00557: metallopeptidase family M24 Length = 441 Score = 134 bits (324), Expect = 6e-32 Identities = 61/109 (55%), Positives = 78/109 (71%) Frame = +2 Query: 233 PDSERWRATRMEENEIYAIETFGSTGRGQVHDDMDCSHYMKNFDQQFVPLRLQSSKQLLN 412 P+ T+MEE E+YAIETFGSTG+G V +D++CSHYMKN+D VPLRL +KQLL Sbjct: 308 PNVRGGEQTKMEEGELYAIETFGSTGKGYVREDLECSHYMKNYDVGHVPLRLPRAKQLLA 367 Query: 413 VINKNFGTLAFCKRWLERAGASRYAMALKDLCTRGWWTPTRPSATSRGA 559 INKNF TLAFC+R+L+R G ++Y MALK+LC G P P +G+ Sbjct: 368 TINKNFSTLAFCRRYLDRLGETKYLMALKNLCDSGIIEPCPPVCDVKGS 416 Score = 128 bits (309), Expect = 4e-30 Identities = 59/84 (70%), Positives = 72/84 (85%) Frame = +3 Query: 6 FNPRYDPLVKGVQEATEAGIKASGVDVRLCDVGAAVQEVMESHEVELDGQMYQVKPIRNL 185 FNP +DPL+ ++AT GIK +GVDVRLCDVGAAVQEVMES+EVE++G++YQVK IRNL Sbjct: 232 FNPMFDPLLAASRDATYTGIKEAGVDVRLCDVGAAVQEVMESYEVEINGKVYQVKSIRNL 291 Query: 186 NGHSIEPYKIHAGKTVPIVKGGEQ 257 NGHSI Y+IHA K+VP V+GGEQ Sbjct: 292 NGHSIGRYQIHAEKSVPNVRGGEQ 315 Score = 58.4 bits (135), Expect = 5e-09 Identities = 26/57 (45%), Positives = 39/57 (68%) Frame = +1 Query: 439 RVLQALAGTRRGLSLRDGAEGPLHKGVVDAYPPLCDIKGCYTAQFEHTILLRPTCKK 609 R L L T+ ++L++ + G+++ PP+CD+KG Y +QFEHTILLRPTCK+ Sbjct: 381 RYLDRLGETKYLMALKNLCDS----GIIEPCPPVCDVKGSYISQFEHTILLRPTCKE 433 >At3g51800.2 68416.m05681 metallopeptidase M24 family protein similar to SP|P50580 Proliferation-associated protein 2G4 {Mus musculus}; contains Pfam profile PF00557: metallopeptidase family M24 Length = 401 Score = 41.5 bits (93), Expect = 6e-04 Identities = 24/72 (33%), Positives = 42/72 (58%), Gaps = 2/72 (2%) Frame = +2 Query: 266 EENEIYAIETFGSTGRG--QVHDDMDCSHYMKNFDQQFVPLRLQSSKQLLNVINKNFGTL 439 EENE+YAI+ STG G ++ D+ + Y K+ + L++++S+ +++ I +NF + Sbjct: 216 EENEVYAIDIVASTGDGKPKLLDEKQTTIYKKDESVNY-QLKMKASRFIISEIKQNFPRM 274 Query: 440 AFCKRWLERAGA 475 F R LE A Sbjct: 275 PFTARSLEEKRA 286 >At3g51800.1 68416.m05680 metallopeptidase M24 family protein similar to SP|P50580 Proliferation-associated protein 2G4 {Mus musculus}; contains Pfam profile PF00557: metallopeptidase family M24 Length = 392 Score = 41.5 bits (93), Expect = 6e-04 Identities = 24/72 (33%), Positives = 42/72 (58%), Gaps = 2/72 (2%) Frame = +2 Query: 266 EENEIYAIETFGSTGRG--QVHDDMDCSHYMKNFDQQFVPLRLQSSKQLLNVINKNFGTL 439 EENE+YAI+ STG G ++ D+ + Y K+ + L++++S+ +++ I +NF + Sbjct: 216 EENEVYAIDIVASTGDGKPKLLDEKQTTIYKKDESVNY-QLKMKASRFIISEIKQNFPRM 274 Query: 440 AFCKRWLERAGA 475 F R LE A Sbjct: 275 PFTARSLEEKRA 286 >At4g30710.2 68417.m04353 expressed protein contains Pfam domain, PF04484: Family of unknown function (DUF566) Length = 644 Score = 29.9 bits (64), Expect = 2.0 Identities = 21/65 (32%), Positives = 28/65 (43%), Gaps = 4/65 (6%) Frame = +2 Query: 524 TPTRPSATSRGATPHSSNTPSC----SDPPARSRVPWR*LLTSDPTNLLNATSYSSTRVN 691 +PTR + SRG +P SC S PP+R P R T+ T TS S + Sbjct: 359 SPTRGLSPSRGLSPSRGTNTSCFARPSTPPSRGISPSRIRQTTTSTQSSTTTSVLSFITD 418 Query: 692 PNSGQ 706 G+ Sbjct: 419 VKKGK 423 >At4g30710.1 68417.m04352 expressed protein contains Pfam domain, PF04484: Family of unknown function (DUF566) Length = 644 Score = 29.9 bits (64), Expect = 2.0 Identities = 21/65 (32%), Positives = 28/65 (43%), Gaps = 4/65 (6%) Frame = +2 Query: 524 TPTRPSATSRGATPHSSNTPSC----SDPPARSRVPWR*LLTSDPTNLLNATSYSSTRVN 691 +PTR + SRG +P SC S PP+R P R T+ T TS S + Sbjct: 359 SPTRGLSPSRGLSPSRGTNTSCFARPSTPPSRGISPSRIRQTTTSTQSSTTTSVLSFITD 418 Query: 692 PNSGQ 706 G+ Sbjct: 419 VKKGK 423 >At1g04160.1 68414.m00406 myosin family protein contains Pfam profiles: PF02736 myosin N-terminal SH3-like domain, PF00063 myosin head (motor domain), PF00612 IQ calmodulin-binding motif, PF01843: DIL domain Length = 1500 Score = 27.9 bits (59), Expect = 7.9 Identities = 17/46 (36%), Positives = 25/46 (54%), Gaps = 1/46 (2%) Frame = +1 Query: 352 EKLRSAICSVEIAIVETASE-RDQQELWYTRVLQALAGTRRGLSLR 486 EKL+ + S+EI I ETA E + + R+ QALA + L+ Sbjct: 980 EKLKGMVSSLEIKIDETAKELHETARISQDRLKQALAAESKVAKLK 1025 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,774,387 Number of Sequences: 28952 Number of extensions: 349686 Number of successful extensions: 1039 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 986 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1036 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1726528800 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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