Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00870
(737 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g03650.1 68418.m00324 1,4-alpha-glucan branching enzyme / sta... 126 1e-29
At2g36390.1 68415.m04466 1,4-alpha-glucan branching enzyme / sta... 124 5e-29
At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein s... 84 8e-17
At2g39930.1 68415.m04907 isoamylase, putative / starch debranchi... 31 1.1
At4g09020.1 68417.m01489 isoamylase, putative / starch debranchi... 30 1.4
At5g42390.1 68418.m05161 metalloendopeptidase identical to chlor... 29 4.3
At5g44490.1 68418.m05451 F-box family protein contains F-box dom... 28 5.6
>At5g03650.1 68418.m00324 1,4-alpha-glucan branching enzyme / starch
branching enzyme class II (SBE2-2) identical to starch
branching enzyme class II [Arabidopsis thaliana]
GI:726490
Length = 716
Score = 126 bits (305), Expect = 1e-29
Identities = 52/83 (62%), Positives = 66/83 (79%)
Frame = +3
Query: 3 LWDSRLFNYSEIEVLRFLLSNLRWYQEEYRFDGFRFDGVTSMLYHSRGIGEGFSGHYDEY 182
+WDSRLFNY EVLR+LLSN RW+ EEY+FDGFRFDGVTSM+Y G+ GF+G+Y EY
Sbjct: 326 MWDSRLFNYGSWEVLRYLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLSVGFTGNYTEY 385
Query: 183 YGLNVDTEALIYLMLANDIVHSI 251
+GL D +A+ YLML ND++H +
Sbjct: 386 FGLETDVDAVNYLMLVNDMIHGL 408
Score = 114 bits (275), Expect = 5e-26
Identities = 54/85 (63%), Positives = 61/85 (71%)
Frame = +2
Query: 263 ITIAEDVSGMPASCRPVREGGTGFDYRLGMAIPDMWIXXXXXXXXXXWKMGHIVHTLTNR 442
IT+ EDVSGMP C PV++GG GFDYRL MAI D WI W+MG I++TLTNR
Sbjct: 413 ITVGEDVSGMPTFCIPVQDGGVGFDYRLHMAIADKWIEMLKKRDED-WQMGDIIYTLTNR 471
Query: 443 RWMEGTIAYAESHDQALVGDKT*RF 517
RW E I+YAESHDQALVGDKT F
Sbjct: 472 RWSEKCISYAESHDQALVGDKTIAF 496
Score = 101 bits (243), Expect = 4e-22
Identities = 50/87 (57%), Positives = 58/87 (66%), Gaps = 11/87 (12%)
Frame = +1
Query: 508 IAFWLMDAAMYTHMSTLSEPSAVVERGLALHCMIRLITHALGGEGYLNFIGNEFGHPEWL 687
IAFWLMD MY M+ + +++RG+ALH MIRLIT LGGEGYLNF+GNEFGHPEW+
Sbjct: 494 IAFWLMDKDMYDFMAVDRPSTPLIDRGIALHKMIRLITMGLGGEGYLNFMGNEFGHPEWI 553
Query: 688 DFPRA-----------GNNSSYHYARR 735
DFPR GNN SY RR
Sbjct: 554 DFPRGEQRLSDGSVIPGNNFSYDKCRR 580
>At2g36390.1 68415.m04466 1,4-alpha-glucan branching enzyme / starch
branching enzyme class II (SBE2-1) nearly identical to
starch branching enzyme class II [Arabidopsis thaliana]
GI:619939
Length = 858
Score = 124 bits (300), Expect = 5e-29
Identities = 50/83 (60%), Positives = 67/83 (80%)
Frame = +3
Query: 3 LWDSRLFNYSEIEVLRFLLSNLRWYQEEYRFDGFRFDGVTSMLYHSRGIGEGFSGHYDEY 182
+WDSRLFNY EVLR+LLSN RW+ EEY+FDGFRFDGVTSM+Y G+ F+G+Y+EY
Sbjct: 450 MWDSRLFNYGSWEVLRYLLSNARWWLEEYKFDGFRFDGVTSMMYTHHGLQVEFTGNYNEY 509
Query: 183 YGLNVDTEALIYLMLANDIVHSI 251
+G + D +A++YLML ND++H +
Sbjct: 510 FGYSTDVDAVVYLMLVNDLIHGL 532
Score = 107 bits (258), Expect = 6e-24
Identities = 51/85 (60%), Positives = 57/85 (67%)
Frame = +2
Query: 263 ITIAEDVSGMPASCRPVREGGTGFDYRLGMAIPDMWIXXXXXXXXXXWKMGHIVHTLTNR 442
I + EDVSGMPA C PV +GG GFDYRL MA+ D WI W++G I TLTNR
Sbjct: 537 IVVGEDVSGMPAFCVPVEDGGVGFDYRLHMAVADKWI-ELLKKRDEDWQVGDITFTLTNR 595
Query: 443 RWMEGTIAYAESHDQALVGDKT*RF 517
RW E + YAESHDQALVGDKT F
Sbjct: 596 RWGEKCVVYAESHDQALVGDKTIAF 620
Score = 104 bits (249), Expect = 7e-23
Identities = 52/87 (59%), Positives = 59/87 (67%), Gaps = 11/87 (12%)
Frame = +1
Query: 508 IAFWLMDAAMYTHMSTLSEPSAVVERGLALHCMIRLITHALGGEGYLNFIGNEFGHPEWL 687
IAFWLMD MY M+ + + V+RG+ALH MIRLIT LGGEGYLNF+GNEFGHPEW+
Sbjct: 618 IAFWLMDKDMYDFMAVDRQATPRVDRGIALHKMIRLITMGLGGEGYLNFMGNEFGHPEWI 677
Query: 688 DFPR-----------AGNNSSYHYARR 735
DFPR AGNN SY RR
Sbjct: 678 DFPRTDQHLPDGRVIAGNNGSYDKCRR 704
>At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein
similar to 1,4-alpha-glucan branching enzyme [Solanum
tuberosum] GI:1621012, 1,4-alpha-glucan branching enzyme
(EC 2.4.1.18) from [Homo sapiens] SP|Q04446, {Solanum
tuberosum} SP|P30924; contains Pfam profiles: PF00128
Alpha amylase catalytic domain, PF02922 Isoamylase
N-terminal domain
Length = 777
Score = 84.2 bits (199), Expect = 8e-17
Identities = 35/80 (43%), Positives = 54/80 (67%)
Frame = +3
Query: 6 WDSRLFNYSEIEVLRFLLSNLRWYQEEYRFDGFRFDGVTSMLYHSRGIGEGFSGHYDEYY 185
W +R+F Y +++VL FL+SNL W+ EY+ DG++F + SM+Y G F+ D+Y
Sbjct: 370 WGTRMFKYGDLDVLHFLISNLNWWITEYQVDGYQFHSLASMIYTHNGFA-SFNNDLDDYC 428
Query: 186 GLNVDTEALIYLMLANDIVH 245
VD +AL+YL+LAN+I+H
Sbjct: 429 NQYVDRDALMYLILANEILH 448
Score = 72.9 bits (171), Expect = 2e-13
Identities = 31/54 (57%), Positives = 40/54 (74%)
Frame = +1
Query: 574 VVERGLALHCMIRLITHALGGEGYLNFIGNEFGHPEWLDFPRAGNNSSYHYARR 735
+++RG++LH MIRLIT GG YLNF+GNEFGHPE ++FP NN S+ A R
Sbjct: 560 LLDRGISLHKMIRLITFTSGGRAYLNFMGNEFGHPERVEFPTQSNNFSFSLANR 613
Score = 66.9 bits (156), Expect = 1e-11
Identities = 31/88 (35%), Positives = 50/88 (56%), Gaps = 5/88 (5%)
Frame = +2
Query: 260 VITIAEDVSGMPASCRPVREGGTGFDYRLGMAIPDMWIXXXXXXXXXXWKMG----HIVH 427
+ITIAED + P C PV +GG GFDY + ++ +MW+ W M IV
Sbjct: 454 IITIAEDATYYPGLCEPVSQGGLGFDYYVNLSASEMWVSLLDNVPDNEWSMSKPVLQIVS 513
Query: 428 TL-TNRRWMEGTIAYAESHDQALVGDKT 508
TL N+ + + ++YAE+H+Q++ G ++
Sbjct: 514 TLVANKEYADKMLSYAENHNQSISGGRS 541
>At2g39930.1 68415.m04907 isoamylase, putative / starch debranching
enzyme, putative similar to isoamylase from [Solanum
tuberosum] GI:27728145, [Triticum aestivum] GI:17932898,
[Hordeum vulgare] GI:21314275, [Oryza sativa]
GI:3252794; contains Pfam profiles PF00128: Alpha
amylase catalytic domain, PF02922: Isoamylase N-terminal
domain
Length = 783
Score = 30.7 bits (66), Expect = 1.1
Identities = 14/31 (45%), Positives = 19/31 (61%)
Frame = +3
Query: 21 FNYSEIEVLRFLLSNLRWYQEEYRFDGFRFD 113
FN + V +F+L LR++ E DGFRFD
Sbjct: 380 FNCNHPVVRQFILDCLRYWVTEMHVDGFRFD 410
>At4g09020.1 68417.m01489 isoamylase, putative / starch debranching
enzyme, putative similar to isoamylase isoform 3
[Solanum tuberosum] GI:27728149, isoamylase [Oryza
sativa] GI:3252794; contains Pfam profiles PF00128:
Alpha amylase catalytic domain, PF02922: Isoamylase
N-terminal domain
Length = 764
Score = 30.3 bits (65), Expect = 1.4
Identities = 12/24 (50%), Positives = 16/24 (66%)
Frame = +3
Query: 42 VLRFLLSNLRWYQEEYRFDGFRFD 113
V+ +L +LR + EY DGFRFD
Sbjct: 405 VMELILDSLRHWVTEYHVDGFRFD 428
>At5g42390.1 68418.m05161 metalloendopeptidase identical to
chloroplast processing enzyme metalloendopeptidase
[Arabidopsis thaliana] gi|2827039|gb|AAC39482
Length = 1265
Score = 28.7 bits (61), Expect = 4.3
Identities = 16/43 (37%), Positives = 24/43 (55%)
Frame = -1
Query: 605 MQCRARPRSTTALGSLRVLMCVYIAASINQNAMFYRQLRLDHD 477
+Q + PR L ++ L+ +Y AASI + Y QLR+D D
Sbjct: 1177 LQASSVPRKE--LSCIKELVSLYEAASIEDIYLAYNQLRVDED 1217
>At5g44490.1 68418.m05451 F-box family protein contains F-box domain
Pfam:PF00646
Length = 455
Score = 28.3 bits (60), Expect = 5.6
Identities = 12/31 (38%), Positives = 18/31 (58%)
Frame = +3
Query: 156 GFSGHYDEYYGLNVDTEALIYLMLANDIVHS 248
GF G+Y +G+ +D L YL + +DI S
Sbjct: 208 GFHGYYSLDFGVLIDAPRLKYLKIGDDISRS 238
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,931,108
Number of Sequences: 28952
Number of extensions: 283556
Number of successful extensions: 676
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 657
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 672
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1624036432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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