BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00857 (646 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 30 0.25 SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 30 0.25 SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyce... 30 0.33 SPBC16E9.16c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 28 1.3 SPBP18G5.02 |||CDP-diacylglycerol-glycerol-3-phosphate3-phosphat... 27 1.8 SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb... 27 1.8 SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase Swr1|Schizo... 27 2.3 SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 27 3.1 SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po... 27 3.1 SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 27 3.1 SPAC694.04c |||conserved eukaryotic protein|Schizosaccharomyces ... 27 3.1 SPCC364.04c |||CASP family protein|Schizosaccharomyces pombe|chr... 26 4.0 SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7 |S... 26 4.0 SPBC8D2.14c |sed5||SNARE Sed5 |Schizosaccharomyces pombe|chr 2||... 26 4.0 SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces pomb... 26 4.0 SPBC649.05 |cut12|stf1|spindle pole body protein Cut12 |Schizosa... 25 7.1 SPBC18H10.19 |||human UVRAG |Schizosaccharomyces pombe|chr 2|||M... 25 7.1 SPBC16H5.05c |cyp7|cwf27|cyclophilin family peptidyl-prolyl cis-... 25 7.1 SPCC188.08c |ubp22|ubp5|ubiquitin C-terminal hydrolase Ubp22|Sch... 25 7.1 SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux transpo... 25 9.3 SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomy... 25 9.3 SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces p... 25 9.3 SPAC22E12.02 |||RNA-binding protein|Schizosaccharomyces pombe|ch... 25 9.3 SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78 |Schizosacch... 25 9.3 SPBC27B12.12c |||CorA family magnesium ion transporter |Schizosa... 25 9.3 SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces pomb... 25 9.3 SPBC21.06c |cdc7|pld1, its10|serine/threonine protein kinase Cdc... 25 9.3 >SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 412 Score = 30.3 bits (65), Expect = 0.25 Identities = 28/93 (30%), Positives = 43/93 (46%) Frame = +1 Query: 16 PHSVSRQYIMAAKFVVLFACIALAQGAMVRRDAPDFFKDIEHHTKEFHKTLEQQFNSLTK 195 P S S + A + L + V R F + +EH+ K+LE+Q + L + Sbjct: 171 PSSSSCNLVNANSLDIYLNINNLKKSKSVPRLRGQFMEPVEHN-HPLSKSLEEQSSFLEQ 229 Query: 196 SKDAQDFSKAWKDGSESVLHSSTPSPRVSRERS 294 SKDA A + S S L S+ S R+S++ S Sbjct: 230 SKDASSNLTAC-NRSGSSLSSNFYSSRLSKKTS 261 >SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 419 Score = 30.3 bits (65), Expect = 0.25 Identities = 17/81 (20%), Positives = 38/81 (46%) Frame = +3 Query: 261 NAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQN 440 N + ++ AL + KA + LE+ ++ E + EE+ H + T+ + + + Sbjct: 337 NLVSLAIYEALYEKFLKACKDLEEVSKSYEESREEIEALHETFTEEVTSFQSTKRLKEEK 396 Query: 441 TVQESQKLAKKVSSNVQETNE 503 +QE ++ K + Q+ +E Sbjct: 397 IIQEKSRVDKMIDEYRQKLSE 417 >SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyces pombe|chr 3|||Manual Length = 238 Score = 29.9 bits (64), Expect = 0.33 Identities = 19/71 (26%), Positives = 37/71 (52%), Gaps = 3/71 (4%) Frame = +3 Query: 261 NAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPD---VEKNATALREKLQAA 431 N + + L + +A KA+++LEQ+ + E L K H + E+ + +EKL A Sbjct: 43 NEYLRQLS-EINEAQKKAEKSLEQTEARKQNFTELLEKEHEEQAITEQEIFSFQEKLDAM 101 Query: 432 VQNTVQESQKL 464 ++ + S++L Sbjct: 102 LKRKQKLSEEL 112 >SPBC16E9.16c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 642 Score = 27.9 bits (59), Expect = 1.3 Identities = 23/57 (40%), Positives = 31/57 (54%), Gaps = 1/57 (1%) Frame = +3 Query: 249 AAQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRK-AHPDVEKNATALRE 416 A +L A AK A DA A E +++ +IER+A K A + E+ ATALRE Sbjct: 140 ALELEARAKK---AAQDAE-LASERAREAQSSIERSASLREKQAREEAERAATALRE 192 >SPBP18G5.02 |||CDP-diacylglycerol-glycerol-3-phosphate3- phosphatidyltransferase|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 27.5 bits (58), Expect = 1.8 Identities = 14/36 (38%), Positives = 21/36 (58%), Gaps = 1/36 (2%) Frame = +2 Query: 170 NNSLTRSPSQRTHRTSARLGRTAPSP-CCTAQRLRQ 274 +N+L+ +PS H + +L T SP CC+A L Q Sbjct: 74 SNALSNNPSLHVHILADQLRCTRESPGCCSASLLMQ 109 >SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 631 Score = 27.5 bits (58), Expect = 1.8 Identities = 10/20 (50%), Positives = 13/20 (65%) Frame = -2 Query: 339 ASTVPKPPWPCRSRLRALPG 280 AST+ K PWP + L +PG Sbjct: 38 ASTLEKEPWPASTALLVMPG 57 >SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase Swr1|Schizosaccharomyces pombe|chr 1|||Manual Length = 1288 Score = 27.1 bits (57), Expect = 2.3 Identities = 23/75 (30%), Positives = 33/75 (44%) Frame = +3 Query: 234 RLRVRAAQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPDVEKNATALR 413 R R + + A K L GA KAKE ++ R +RTA E+RK +E+ R Sbjct: 142 RSRRISGMILAHFKRLSGA---DEKKAKEEDKRIRLLAKRTAWEIRKKWKVIEREVRRRR 198 Query: 414 EKLQAAVQNTVQESQ 458 + A Q + Q Sbjct: 199 AERAAEAQRVAGKEQ 213 >SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces pombe|chr 2|||Manual Length = 1485 Score = 26.6 bits (56), Expect = 3.1 Identities = 12/42 (28%), Positives = 19/42 (45%), Gaps = 2/42 (4%) Frame = +1 Query: 109 DAPDFFKDIEHHTKEFHKTLEQ--QFNSLTKSKDAQDFSKAW 228 D +F D++ H K FH E+ + + +K D K W Sbjct: 665 DMKSYFSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEW 706 >SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces pombe|chr 1|||Manual Length = 670 Score = 26.6 bits (56), Expect = 3.1 Identities = 17/57 (29%), Positives = 26/57 (45%), Gaps = 5/57 (8%) Frame = +3 Query: 276 SLQGALGDANGKAKEALEQSRQNIE-----RTAEELRKAHPDVEKNATALREKLQAA 431 S++ L + N + KE +E + RT +E EKN LRE+L+ A Sbjct: 520 SMKDDLTEMNQRLKEQIESYENEVNSEITSRTLKEFETLKTQYEKNLCNLREQLKTA 576 >SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizosaccharomyces pombe|chr 1|||Manual Length = 899 Score = 26.6 bits (56), Expect = 3.1 Identities = 10/30 (33%), Positives = 18/30 (60%) Frame = +1 Query: 103 RRDAPDFFKDIEHHTKEFHKTLEQQFNSLT 192 ++ DFFK + ++ H TL ++ NSL+ Sbjct: 56 KKSEQDFFKMLSSRDRDAHSTLRKRSNSLS 85 >SPAC694.04c |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 324 Score = 26.6 bits (56), Expect = 3.1 Identities = 14/61 (22%), Positives = 28/61 (45%) Frame = +1 Query: 118 DFFKDIEHHTKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLHSSTPSPRVSRERSE 297 D K +HH +EF+ T ++++ S +K V+H+ P +++ + E Sbjct: 59 DGIKYFDHHQREFNDTFSPKYSTRLSSA-----GLIYKHFGREVIHAVLPQLKINEQDLE 113 Query: 298 T 300 T Sbjct: 114 T 114 >SPCC364.04c |||CASP family protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 633 Score = 26.2 bits (55), Expect = 4.0 Identities = 23/75 (30%), Positives = 35/75 (46%), Gaps = 8/75 (10%) Frame = +3 Query: 309 KAKEALE---QSRQNIERTAEELRKAHPDVEKNATALREKLQAAV-----QNTVQESQKL 464 K KE +E Q ++ +ER E LRK D K+ + +AA+ + + E QKL Sbjct: 128 KEKEEMEGSLQGKEKLEREVENLRK-ELDKYKDLVETEAEKRAAITKEECEKSWLEQQKL 186 Query: 465 AKKVSSNVQETNEKL 509 K + T +KL Sbjct: 187 YKDMEQENASTIQKL 201 >SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7 |Schizosaccharomyces pombe|chr 2|||Manual Length = 272 Score = 26.2 bits (55), Expect = 4.0 Identities = 15/59 (25%), Positives = 34/59 (57%) Frame = +3 Query: 309 KAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSN 485 K+++ LE S Q +E E + P+V++ +EK ++ V+ +E +K+++ + S+ Sbjct: 132 KSEKPLETS-QKVEIETVETKPGEPEVKQETNLQKEKKESKVKLESKE-EKISRNLRSS 188 >SPBC8D2.14c |sed5||SNARE Sed5 |Schizosaccharomyces pombe|chr 2|||Manual Length = 309 Score = 26.2 bits (55), Expect = 4.0 Identities = 21/70 (30%), Positives = 34/70 (48%), Gaps = 4/70 (5%) Frame = +3 Query: 213 LQQGLEGRLRVRAAQLNAFAK----SLQGALGDANGKAKEALEQSRQNIERTAEELRKAH 380 LQQ ++G R + AQ+N ++ SLQ +L + + K+ LE QN++ + K Sbjct: 102 LQQVVKGN-RNKPAQMNQHSENVVVSLQNSLANTSMTFKDILEIRTQNMKASQNRTEKFV 160 Query: 381 PDVEKNATAL 410 NA L Sbjct: 161 ASSSMNANPL 170 >SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces pombe|chr 2|||Manual Length = 175 Score = 26.2 bits (55), Expect = 4.0 Identities = 10/24 (41%), Positives = 18/24 (75%) Frame = +3 Query: 324 LEQSRQNIERTAEELRKAHPDVEK 395 +EQ+R E T E++++A P++EK Sbjct: 126 IEQARPTEEITIEDMKQAVPEIEK 149 >SPBC649.05 |cut12|stf1|spindle pole body protein Cut12 |Schizosaccharomyces pombe|chr 2|||Manual Length = 548 Score = 25.4 bits (53), Expect = 7.1 Identities = 10/29 (34%), Positives = 18/29 (62%) Frame = +3 Query: 366 LRKAHPDVEKNATALREKLQAAVQNTVQE 452 L AH D+E T++RE+L++ + +E Sbjct: 267 LDSAHSDLELELTSIRERLESLILEKQEE 295 >SPBC18H10.19 |||human UVRAG |Schizosaccharomyces pombe|chr 2|||Manual Length = 307 Score = 25.4 bits (53), Expect = 7.1 Identities = 10/34 (29%), Positives = 19/34 (55%) Frame = +1 Query: 169 EQQFNSLTKSKDAQDFSKAWKDGSESVLHSSTPS 270 ++Q NS+T+ + D +K+W + L+ S S Sbjct: 84 QKQINSITQMRTKFDKTKSWLSSKRNTLNKSKES 117 >SPBC16H5.05c |cyp7|cwf27|cyclophilin family peptidyl-prolyl cis-trans isomerase Cyp7|Schizosaccharomyces pombe|chr 2|||Manual Length = 463 Score = 25.4 bits (53), Expect = 7.1 Identities = 18/62 (29%), Positives = 28/62 (45%), Gaps = 3/62 (4%) Frame = +1 Query: 127 KDIEHHTKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLHSSTPS---PRVSRERSE 297 KD+ + H T LT +K + D D ++ L+SS+ S +SR +SE Sbjct: 246 KDLISSSSSIHSTYSSAQTGLTSAKVSSDEYARQVDTLDTKLNSSSKSKVQEEISRLKSE 305 Query: 298 TR 303 R Sbjct: 306 LR 307 >SPCC188.08c |ubp22|ubp5|ubiquitin C-terminal hydrolase Ubp22|Schizosaccharomyces pombe|chr 3|||Manual Length = 1108 Score = 25.4 bits (53), Expect = 7.1 Identities = 13/32 (40%), Positives = 19/32 (59%) Frame = +3 Query: 303 NGKAKEALEQSRQNIERTAEELRKAHPDVEKN 398 N K KE E++ + EELR+A PD E++ Sbjct: 22 NEKLKEDFEENVSIDVKIHEELRRALPDYEES 53 >SPCC18B5.01c |bfr1|hba2, SPCPJ732.04c|brefeldin A efflux transporter Bfr1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1530 Score = 25.0 bits (52), Expect = 9.3 Identities = 13/26 (50%), Positives = 16/26 (61%) Frame = -1 Query: 418 FSRRAVAFFSTSGWALRSSSAVRSMF 341 FSR V FFS AL+S S + +MF Sbjct: 570 FSRGGVLFFSILFCALQSLSEIANMF 595 >SPCC417.07c |mto1|mbo1, mod20|MT organizer Mto1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1115 Score = 25.0 bits (52), Expect = 9.3 Identities = 22/101 (21%), Positives = 52/101 (51%), Gaps = 2/101 (1%) Frame = +3 Query: 213 LQQGLEGRLRVRAAQLNAF--AKSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPD 386 ++Q E + + + ++NA A+S + L D+ ++ ++ Q+IE T L K D Sbjct: 459 VKQVEELTVALNSGKMNAIVEAESSKNELWDSMMVSR--MKTQEQSIELT--RLYKQLQD 514 Query: 387 VEKNATALREKLQAAVQNTVQESQKLAKKVSSNVQETNEKL 509 +E++ +++ + V + Q+ ++++ +Q+T E L Sbjct: 515 IEEDYENKLMRMEQQWREDVDQLQEYVEEITQELQDTKEVL 555 >SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces pombe|chr 1|||Manual Length = 521 Score = 25.0 bits (52), Expect = 9.3 Identities = 10/24 (41%), Positives = 15/24 (62%) Frame = +3 Query: 294 GDANGKAKEALEQSRQNIERTAEE 365 G NG+AKE E+ R ++T E+ Sbjct: 473 GKGNGRAKETPEEKRARKKKTKED 496 >SPAC22E12.02 |||RNA-binding protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 219 Score = 25.0 bits (52), Expect = 9.3 Identities = 10/38 (26%), Positives = 24/38 (63%) Frame = +3 Query: 6 RISSALSLSTVHHGRQVRSSLRLHRSGPRSDGATRRSR 119 +I ++L T+HHG +V+ ++ ++ G S ++R ++ Sbjct: 122 KIRNSLYSRTIHHGNRVQKKIK-NKHGKNSSKSSRAAQ 158 >SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78 |Schizosaccharomyces pombe|chr 2|||Manual Length = 803 Score = 25.0 bits (52), Expect = 9.3 Identities = 12/23 (52%), Positives = 15/23 (65%) Frame = +3 Query: 297 DANGKAKEALEQSRQNIERTAEE 365 D+ KAK LEQ+R +ERT E Sbjct: 403 DSIKKAKTELEQARIELERTQRE 425 >SPBC27B12.12c |||CorA family magnesium ion transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 803 Score = 25.0 bits (52), Expect = 9.3 Identities = 10/24 (41%), Positives = 14/24 (58%) Frame = -2 Query: 345 CSASTVPKPPWPCRSRLRALPGDS 274 C +T+PKPP+ + L LP S Sbjct: 290 CPHTTIPKPPYQSDTDLTELPTKS 313 >SPAC144.17c |||6-phosphofructo-2-kinase|Schizosaccharomyces pombe|chr 1|||Manual Length = 432 Score = 25.0 bits (52), Expect = 9.3 Identities = 13/51 (25%), Positives = 26/51 (50%) Frame = +3 Query: 330 QSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSS 482 ++RQ E A+ +RK+ PDV + ++ +V + +E + +SS Sbjct: 284 ENRQFSEYVADRIRKSFPDVSFKNLHVLSCMEDSVMSPFRELGSVTSSMSS 334 >SPBC21.06c |cdc7|pld1, its10|serine/threonine protein kinase Cdc7|Schizosaccharomyces pombe|chr 2|||Manual Length = 1062 Score = 25.0 bits (52), Expect = 9.3 Identities = 15/47 (31%), Positives = 24/47 (51%) Frame = +1 Query: 130 DIEHHTKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLHSSTPS 270 D ++ EF TL+ + L KS+ DF +K + S +S+PS Sbjct: 325 DHDNWDNEFQGTLKISDDVLKKSEHFMDFCSNFKGKNNSSSITSSPS 371 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,022,021 Number of Sequences: 5004 Number of extensions: 34244 Number of successful extensions: 176 Number of sequences better than 10.0: 27 Number of HSP's better than 10.0 without gapping: 168 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 175 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 289756512 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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