BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00854 (776 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC3H8.10 |spo20|sec14|sec14 cytosolic factor family Sec14|Schi... 31 0.14 SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 26 5.2 SPAC19G12.08 |||fatty acid hydroxylase |Schizosaccharomyces pomb... 26 6.9 >SPAC3H8.10 |spo20|sec14|sec14 cytosolic factor family Sec14|Schizosaccharomyces pombe|chr 1|||Manual Length = 286 Score = 31.5 bits (68), Expect = 0.14 Identities = 15/50 (30%), Positives = 25/50 (50%) Frame = +2 Query: 110 QDCMPVRFKSIHFIGQPWYVETALAVIKPYLKAKTRERIVLHGNNLSTPM 259 QD P R + I PW +A +IK +L T ++I + G+N + + Sbjct: 197 QDYYPERMGKFYVINAPWGFSSAFNLIKGFLDEATVKKIHILGSNYKSAL 246 >SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1158 Score = 26.2 bits (55), Expect = 5.2 Identities = 14/34 (41%), Positives = 18/34 (52%) Frame = +3 Query: 411 SIGELNKAYIQKDSANFSYHANEKSAKSELLREK 512 +IG+L Y+ K Y AN+ AK E REK Sbjct: 525 TIGDLFLDYVPKFEPFIKYGANQAIAKFEFEREK 558 >SPAC19G12.08 |||fatty acid hydroxylase |Schizosaccharomyces pombe|chr 1|||Manual Length = 347 Score = 25.8 bits (54), Expect = 6.9 Identities = 9/35 (25%), Positives = 19/35 (54%) Frame = +1 Query: 547 YIDSSLPGHATGISIRFAWFGMHHVISSVIYLCII 651 ++D P H +++ FA+ G HH + + Y ++ Sbjct: 209 HLDEYTPDHPIFLTMHFAFHGCHHFLPADKYRLVM 243 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,263,799 Number of Sequences: 5004 Number of extensions: 69292 Number of successful extensions: 162 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 158 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 162 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 375345278 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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