Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00854
(776 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g01630.1 68414.m00080 SEC14 cytosolic factor, putative / phos... 43 2e-04
At1g72160.1 68414.m08343 SEC14 cytosolic factor family protein /... 33 0.21
At4g00020.1 68417.m00002 BRCA2 repeat-containing protein low sim... 31 0.64
At3g51670.1 68416.m05666 SEC14 cytosolic factor family protein /... 31 0.64
At4g09160.1 68417.m01517 SEC14 cytosolic factor family protein /... 31 0.85
At1g30690.1 68414.m03752 SEC14 cytosolic factor family protein /... 31 0.85
At1g08860.1 68414.m00987 copine, putative Similar to BONZAI1 [Ar... 31 0.85
At5g01630.1 68418.m00079 BRCA2 repeat-containing protein low sim... 29 3.4
At4g00420.2 68417.m00058 double-stranded RNA-binding domain (DsR... 29 3.4
At4g00420.1 68417.m00057 double-stranded RNA-binding domain (DsR... 29 3.4
At4g20190.1 68417.m02952 hypothetical protein 29 4.5
At2g45540.1 68415.m05663 WD-40 repeat family protein / beige-rel... 28 7.9
At1g17770.1 68414.m02199 SET domain-containing protein (SUVH7) c... 28 7.9
>At1g01630.1 68414.m00080 SEC14 cytosolic factor, putative /
phosphoglyceride transfer protein, putative contains
Pfam PF00650 : CRAL/TRIO domain and PF03765 : CRAL/TRIO,
N-terminus; similar to polyphosphoinositide binding
protein Ssh2p GB:AAB94599 GI:2739046 from [Glycine max]
Length = 255
Score = 43.2 bits (97), Expect = 2e-04
Identities = 20/56 (35%), Positives = 30/56 (53%)
Frame = +2
Query: 98 IDCLQDCMPVRFKSIHFIGQPWYVETALAVIKPYLKAKTRERIVLHGNNLSTPMTL 265
+ LQDC P R ++ + P+ TA VI P++ A T+++IV N TP L
Sbjct: 176 LSTLQDCYPERLGKLYIVHAPYIFMTAWKVIYPFIDANTKKKIVFVENKKLTPTLL 231
>At1g72160.1 68414.m08343 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein similar to
GI:807956 from [Saccharomyces cerevisiae]similar to
polyphosphoinositide binding protein Ssh2p (GI:2739046)
{Glycine max}; contains Pfam PF00650 : CRAL/TRIO domain;
contains Pfam PF03765 : CRAL/TRIO, N-terminus
Length = 490
Score = 33.1 bits (72), Expect = 0.21
Identities = 19/64 (29%), Positives = 31/64 (48%)
Frame = +2
Query: 89 KMMIDCLQDCMPVRFKSIHFIGQPWYVETALAVIKPYLKAKTRERIVLHGNNLSTPMTLY 268
K ++ LQD P FI PW+ VI P++ +++ ++V G + S TL+
Sbjct: 299 KQAVELLQDNYPEFVFKQAFINVPWWYLVFYTVIGPFMTPRSKSKLVFAGPSRSAE-TLF 357
Query: 269 LLIS 280
IS
Sbjct: 358 KYIS 361
>At4g00020.1 68417.m00002 BRCA2 repeat-containing protein low
similarity to SP|P51587 Breast cancer type 2
susceptibility protein {Homo sapiens}; contains Pfam
profile PF00634: BRCA2 repeat
Length = 765
Score = 31.5 bits (68), Expect = 0.64
Identities = 15/40 (37%), Positives = 20/40 (50%)
Frame = -3
Query: 450 CPFVYRLCLVRRWKVVLYCYSCHWCCKCRRNVFTSTELLE 331
C + C+ WK L CY ++ KCR N T T +LE
Sbjct: 570 CDRSFEACMWIVWK--LACYDIYYPAKCRGNFLTITNVLE 607
>At3g51670.1 68416.m05666 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein similar to
polyphosphoinositide binding protein Ssh2p (GI:2739046)
{Glycine max};; contains Pfam PF00650 : CRAL/TRIO
domain; contains Pfam PF03765 : CRAL/TRIO, N-terminus
Length = 409
Score = 31.5 bits (68), Expect = 0.64
Identities = 16/61 (26%), Positives = 27/61 (44%)
Frame = +2
Query: 95 MIDCLQDCMPVRFKSIHFIGQPWYVETALAVIKPYLKAKTRERIVLHGNNLSTPMTLYLL 274
++ QD P + FI PWY ++ P+L +T+ + V+ + TLY
Sbjct: 218 ILSLFQDNYPELVATKIFINVPWYFSVIYSMFSPFLTQRTKSKFVMSKEG-NAAETLYKF 276
Query: 275 I 277
I
Sbjct: 277 I 277
>At4g09160.1 68417.m01517 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein similar to
polyphosphoinositide binding protein Ssh1p (GI:2739044)
{Glycine max}; similar to polyphosphoinositide binding
protein Ssh2, Glycine max, gb:T05953; contains Pfam
PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminus
Length = 668
Score = 31.1 bits (67), Expect = 0.85
Identities = 15/59 (25%), Positives = 26/59 (44%)
Frame = +2
Query: 89 KMMIDCLQDCMPVRFKSIHFIGQPWYVETALAVIKPYLKAKTRERIVLHGNNLSTPMTL 265
K + LQD P FI PW+ +I P++ +++ ++V G + S L
Sbjct: 474 KQALHLLQDNYPEFVSKQIFINVPWWYLAFYRIISPFMSQRSKSKLVFAGPSRSAETLL 532
>At1g30690.1 68414.m03752 SEC14 cytosolic factor family protein /
phosphoglyceride transfer family protein contains Pfam
PF00650 : CRAL/TRIO domain; contains Pfam PF03765 :
CRAL/TRIO, N-terminus; similar to cytosolic factor
(Phosphatidylinositol/phosphatidylcholine transfer
protein) (PI/PCTP) (SP:P24280) [Saccharomyces
cerevisiae]; ESTs gb|T76582, gb|N06574 and gb|Z25700
come from this gene
Length = 540
Score = 31.1 bits (67), Expect = 0.85
Identities = 15/49 (30%), Positives = 26/49 (53%)
Frame = +2
Query: 86 LKMMIDCLQDCMPVRFKSIHFIGQPWYVETALAVIKPYLKAKTRERIVL 232
+K +I+ LQD P FI P++ AV+ P+L +T+ + V+
Sbjct: 349 IKKVIETLQDNYPEFVSRNIFINVPFWFYAMRAVLSPFLTQRTKSKFVV 397
>At1g08860.1 68414.m00987 copine, putative Similar to BONZAI1
[Arabidopsis thaliana] GI:15487382; contains Pfam
profile PF00168: C2 domain
Length = 589
Score = 31.1 bits (67), Expect = 0.85
Identities = 17/53 (32%), Positives = 30/53 (56%), Gaps = 6/53 (11%)
Frame = +3
Query: 372 YNTSDSYNSRGRP--SIGELNKAYIQKDSANFSY----HANEKSAKSELLREK 512
+NTS ++ G+ S+ EL + +QK++ANF Y H K K +L+ ++
Sbjct: 277 FNTSGNHELIGKTEKSVAELERLCLQKEAANFVYPSLSHGRNKVLKGQLIVDR 329
>At5g01630.1 68418.m00079 BRCA2 repeat-containing protein low
similarity to breast cancer susceptibility protein
[Gallus gallus] GI:19568157; contains Pfam profile
PF00634: BRCA2 repeat
Length = 1155
Score = 29.1 bits (62), Expect = 3.4
Identities = 14/30 (46%), Positives = 17/30 (56%), Gaps = 1/30 (3%)
Frame = -3
Query: 417 RWKV-VLYCYSCHWCCKCRRNVFTSTELLE 331
RW V L CY ++ KCR N T T +LE
Sbjct: 583 RWIVWKLACYDIYYPAKCRGNFLTITNVLE 612
>At4g00420.2 68417.m00058 double-stranded RNA-binding domain
(DsRBD)-containing protein contains Pfam profile
PF00035: Double-stranded RNA binding motif
Length = 190
Score = 29.1 bits (62), Expect = 3.4
Identities = 14/37 (37%), Positives = 17/37 (45%)
Frame = -3
Query: 441 VYRLCLVRRWKVVLYCYSCHWCCKCRRNVFTSTELLE 331
+Y LC VR WK LY Y C +FT +E
Sbjct: 89 LYNLCSVRHWKAPLYEYIAEG--PCHMKIFTGKVTVE 123
>At4g00420.1 68417.m00057 double-stranded RNA-binding domain
(DsRBD)-containing protein contains Pfam profile
PF00035: Double-stranded RNA binding motif
Length = 189
Score = 29.1 bits (62), Expect = 3.4
Identities = 14/37 (37%), Positives = 17/37 (45%)
Frame = -3
Query: 441 VYRLCLVRRWKVVLYCYSCHWCCKCRRNVFTSTELLE 331
+Y LC VR WK LY Y C +FT +E
Sbjct: 88 LYNLCSVRHWKAPLYEYIAEG--PCHMKIFTGKVTVE 122
>At4g20190.1 68417.m02952 hypothetical protein
Length = 389
Score = 28.7 bits (61), Expect = 4.5
Identities = 10/35 (28%), Positives = 22/35 (62%)
Frame = +2
Query: 236 GNNLSTPMTLYLLISYRPNLEAKVRHIIQSVGSKS 340
GN+ P++ + PNL+ +++ ++++ GSKS
Sbjct: 307 GNDQDDPVSAAFVFDKEPNLDKEIKGVLKTSGSKS 341
>At2g45540.1 68415.m05663 WD-40 repeat family protein / beige-related
contains Pfam PF02138: Beige/BEACH domain; contains Pfam
PF00400: WD domain, G-beta repeat (3 copies)
Length = 2946
Score = 27.9 bits (59), Expect = 7.9
Identities = 18/46 (39%), Positives = 20/46 (43%), Gaps = 3/46 (6%)
Frame = -1
Query: 374 VSVGGMFSLRQNSWSQRSELYDG---PSPPSSAGKISRGKASWE*T 246
VS G W RS L+ G PS PSS G G SW+ T
Sbjct: 1475 VSYGSCAMDLAEGWKYRSRLWYGVGLPSKPSSLGGGGSGSDSWKST 1520
>At1g17770.1 68414.m02199 SET domain-containing protein (SUVH7)
contains Pfam profiles: PF05033: Pre-SET motif, PF00856
SET domain; identical to cDNA SUVH7 (SUVH7) GI:13517754
Length = 693
Score = 27.9 bits (59), Expect = 7.9
Identities = 11/22 (50%), Positives = 12/22 (54%)
Frame = -1
Query: 335 WSQRSELYDGPSPPSSAGKISR 270
W E YDG + PSS G I R
Sbjct: 58 WPSNKEWYDGDAGPSSTGPIKR 79
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,800,878
Number of Sequences: 28952
Number of extensions: 351639
Number of successful extensions: 926
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 896
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 926
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1736283200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -