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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00850
         (774 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g67270.1 68418.m08480 microtubule-associated EB1 family prote...    95   6e-20
At5g62500.1 68418.m07844 microtubule-associated EB1 family prote...    95   6e-20
At3g47690.1 68416.m05194 microtubule-associated EB1 family prote...    94   8e-20
At2g03200.1 68415.m00273 aspartyl protease family protein contai...    30   1.5  
At4g30990.1 68417.m04398 expressed protein ; expression supporte...    28   7.9  

>At5g67270.1 68418.m08480 microtubule-associated EB1 family protein
           similar to SP|Q9UPY8 Microtubule-associated protein
           RP/EB family member 3 (Protein EB3) {Homo sapiens};
           contains Pfam profiles PF00307: Calponin homology (CH)
           domain, PF03271: EB1 protein
          Length = 329

 Score = 94.7 bits (225), Expect = 6e-20
 Identities = 41/92 (44%), Positives = 59/92 (64%)
 Frame = +1

Query: 226 VKLCEDRRVVTGAAYCQFMDMLFPGSVPMKRIKFKTNLEHEYIQNFKILQAGFKKMGVDK 405
           + L +     +GA +CQ MD + PG+VPM ++ F    E+E IQN+K+LQ  F K+ + K
Sbjct: 29  LNLSKVEEACSGAVHCQLMDSVHPGTVPMHKVNFDAKSEYEMIQNYKVLQDVFNKLKITK 88

Query: 406 IVPIDKLVKGRFQDNFEFLQWFKKFFDANYGG 501
            + + KLVKGR  DN EF+QW KK+ D+  GG
Sbjct: 89  HIEVSKLVKGRPLDNLEFMQWMKKYCDSVNGG 120



 Score = 31.1 bits (67), Expect = 0.85
 Identities = 11/23 (47%), Positives = 17/23 (73%)
 Frame = +2

Query: 182 LSRHDMLAWVNDCLQSNFAKIEE 250
           + R ++LAW+N  LQ N +K+EE
Sbjct: 14  VGRSEILAWINSTLQLNLSKVEE 36


>At5g62500.1 68418.m07844 microtubule-associated EB1 family protein
           similar to EBF3-S (Microtubule-associated protein) [Homo
           sapiens] GI:12751131; contains Pfam profiles PF00307:
           Calponin homology (CH) domain, PF03271: EB1 protein
          Length = 293

 Score = 94.7 bits (225), Expect = 6e-20
 Identities = 44/109 (40%), Positives = 64/109 (58%), Gaps = 1/109 (0%)
 Frame = +1

Query: 226 VKLCEDRRVVTGAAYCQFMDMLFPGSVPMKRIKFKTNLEHEYIQNFKILQAGFKKMGVDK 405
           + L       +GA  CQ +DM FPG VPM ++ F+   E+E IQN+K++Q  F K+ + K
Sbjct: 29  LNLSRIEEAASGAVQCQMLDMTFPGVVPMHKVNFEAKNEYEMIQNYKVMQEVFTKLKITK 88

Query: 406 IVPIDKLVKGRFQDNFEFLQWFKKFFDANYGGAALTQWR-TRRSAHGAR 549
            + +++LVKGR  DN EFLQW K+F D+  GG     +    R + G R
Sbjct: 89  PLEVNRLVKGRPLDNLEFLQWLKRFCDSINGGIMNENYNPVERRSRGGR 137



 Score = 29.5 bits (63), Expect = 2.6
 Identities = 10/23 (43%), Positives = 18/23 (78%)
 Frame = +2

Query: 182 LSRHDMLAWVNDCLQSNFAKIEE 250
           + R+++L+W+ND L  N ++IEE
Sbjct: 14  VGRNEILSWINDRLHLNLSRIEE 36


>At3g47690.1 68416.m05194 microtubule-associated EB1 family protein
           similar to SP|Q9UPY8 Microtubule-associated protein
           RP/EB family member 3 (Protein EB3) {Homo sapiens};
           contains Pfam profile PF03271: EB1 protein
          Length = 276

 Score = 94.3 bits (224), Expect = 8e-20
 Identities = 46/108 (42%), Positives = 64/108 (59%), Gaps = 2/108 (1%)
 Frame = +1

Query: 226 VKLCEDRRVVTGAAYCQFMDMLFPGSVPMKRIKFKTNLEHEYIQNFKILQAGFKKMGVDK 405
           + L       +GA  CQ +DM FPG VPM ++ F    E++ IQN+K+LQ  F K+ + K
Sbjct: 29  LNLSRVEEAASGAVQCQMLDMTFPGVVPMHKVNFDAKNEYDMIQNYKVLQDVFNKLKITK 88

Query: 406 IVPIDKLVKGRFQDNFEFLQWFKKFFDANYGGAALTQWR--TRRSAHG 543
            + I++LVKGR  DN EFLQW K+F D+  GG     +    RRS +G
Sbjct: 89  PLEINRLVKGRPLDNLEFLQWLKRFCDSINGGIMNENYNPVERRSRNG 136



 Score = 28.7 bits (61), Expect = 4.5
 Identities = 9/23 (39%), Positives = 17/23 (73%)
 Frame = +2

Query: 182 LSRHDMLAWVNDCLQSNFAKIEE 250
           + R+++L W+ND L  N +++EE
Sbjct: 14  VGRNEILTWINDRLHLNLSRVEE 36


>At2g03200.1 68415.m00273 aspartyl protease family protein contains
           Pfam domain, PF00026: eukaryotic aspartyl protease
          Length = 461

 Score = 30.3 bits (65), Expect = 1.5
 Identities = 14/30 (46%), Positives = 18/30 (60%), Gaps = 1/30 (3%)
 Frame = -3

Query: 631 NGLGSSCDFGDRGDGFLSGSGPRG-GRAPV 545
           +G+G  C   + GDGF  GSG  G GR P+
Sbjct: 212 SGIGFGCGVENEGDGFSQGSGLVGLGRGPL 241


>At4g30990.1 68417.m04398 expressed protein ; expression supported by
            MPSS
          Length = 2138

 Score = 27.9 bits (59), Expect = 7.9
 Identities = 18/66 (27%), Positives = 28/66 (42%)
 Frame = -1

Query: 429  HQFVNGYYLVHTHLFKTSL*YFKVLYIFMFQICLKFDSFHWYTAREQHVHELAIGGTCNN 250
            H     YY +    F+    + K   I +  ICL  D FH+  A++ + HE     TC  
Sbjct: 1514 HMSWTSYYALLNRCFREMNKHTKKGKILLRLICLILDKFHF--AKDGYPHEAEEIRTCLQ 1571

Query: 249  SSIFAK 232
              +F +
Sbjct: 1572 KIVFPR 1577


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,244,466
Number of Sequences: 28952
Number of extensions: 370773
Number of successful extensions: 962
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 929
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 962
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1726528800
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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