BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00850 (774 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g67270.1 68418.m08480 microtubule-associated EB1 family prote... 95 6e-20 At5g62500.1 68418.m07844 microtubule-associated EB1 family prote... 95 6e-20 At3g47690.1 68416.m05194 microtubule-associated EB1 family prote... 94 8e-20 At2g03200.1 68415.m00273 aspartyl protease family protein contai... 30 1.5 At4g30990.1 68417.m04398 expressed protein ; expression supporte... 28 7.9 >At5g67270.1 68418.m08480 microtubule-associated EB1 family protein similar to SP|Q9UPY8 Microtubule-associated protein RP/EB family member 3 (Protein EB3) {Homo sapiens}; contains Pfam profiles PF00307: Calponin homology (CH) domain, PF03271: EB1 protein Length = 329 Score = 94.7 bits (225), Expect = 6e-20 Identities = 41/92 (44%), Positives = 59/92 (64%) Frame = +1 Query: 226 VKLCEDRRVVTGAAYCQFMDMLFPGSVPMKRIKFKTNLEHEYIQNFKILQAGFKKMGVDK 405 + L + +GA +CQ MD + PG+VPM ++ F E+E IQN+K+LQ F K+ + K Sbjct: 29 LNLSKVEEACSGAVHCQLMDSVHPGTVPMHKVNFDAKSEYEMIQNYKVLQDVFNKLKITK 88 Query: 406 IVPIDKLVKGRFQDNFEFLQWFKKFFDANYGG 501 + + KLVKGR DN EF+QW KK+ D+ GG Sbjct: 89 HIEVSKLVKGRPLDNLEFMQWMKKYCDSVNGG 120 Score = 31.1 bits (67), Expect = 0.85 Identities = 11/23 (47%), Positives = 17/23 (73%) Frame = +2 Query: 182 LSRHDMLAWVNDCLQSNFAKIEE 250 + R ++LAW+N LQ N +K+EE Sbjct: 14 VGRSEILAWINSTLQLNLSKVEE 36 >At5g62500.1 68418.m07844 microtubule-associated EB1 family protein similar to EBF3-S (Microtubule-associated protein) [Homo sapiens] GI:12751131; contains Pfam profiles PF00307: Calponin homology (CH) domain, PF03271: EB1 protein Length = 293 Score = 94.7 bits (225), Expect = 6e-20 Identities = 44/109 (40%), Positives = 64/109 (58%), Gaps = 1/109 (0%) Frame = +1 Query: 226 VKLCEDRRVVTGAAYCQFMDMLFPGSVPMKRIKFKTNLEHEYIQNFKILQAGFKKMGVDK 405 + L +GA CQ +DM FPG VPM ++ F+ E+E IQN+K++Q F K+ + K Sbjct: 29 LNLSRIEEAASGAVQCQMLDMTFPGVVPMHKVNFEAKNEYEMIQNYKVMQEVFTKLKITK 88 Query: 406 IVPIDKLVKGRFQDNFEFLQWFKKFFDANYGGAALTQWR-TRRSAHGAR 549 + +++LVKGR DN EFLQW K+F D+ GG + R + G R Sbjct: 89 PLEVNRLVKGRPLDNLEFLQWLKRFCDSINGGIMNENYNPVERRSRGGR 137 Score = 29.5 bits (63), Expect = 2.6 Identities = 10/23 (43%), Positives = 18/23 (78%) Frame = +2 Query: 182 LSRHDMLAWVNDCLQSNFAKIEE 250 + R+++L+W+ND L N ++IEE Sbjct: 14 VGRNEILSWINDRLHLNLSRIEE 36 >At3g47690.1 68416.m05194 microtubule-associated EB1 family protein similar to SP|Q9UPY8 Microtubule-associated protein RP/EB family member 3 (Protein EB3) {Homo sapiens}; contains Pfam profile PF03271: EB1 protein Length = 276 Score = 94.3 bits (224), Expect = 8e-20 Identities = 46/108 (42%), Positives = 64/108 (59%), Gaps = 2/108 (1%) Frame = +1 Query: 226 VKLCEDRRVVTGAAYCQFMDMLFPGSVPMKRIKFKTNLEHEYIQNFKILQAGFKKMGVDK 405 + L +GA CQ +DM FPG VPM ++ F E++ IQN+K+LQ F K+ + K Sbjct: 29 LNLSRVEEAASGAVQCQMLDMTFPGVVPMHKVNFDAKNEYDMIQNYKVLQDVFNKLKITK 88 Query: 406 IVPIDKLVKGRFQDNFEFLQWFKKFFDANYGGAALTQWR--TRRSAHG 543 + I++LVKGR DN EFLQW K+F D+ GG + RRS +G Sbjct: 89 PLEINRLVKGRPLDNLEFLQWLKRFCDSINGGIMNENYNPVERRSRNG 136 Score = 28.7 bits (61), Expect = 4.5 Identities = 9/23 (39%), Positives = 17/23 (73%) Frame = +2 Query: 182 LSRHDMLAWVNDCLQSNFAKIEE 250 + R+++L W+ND L N +++EE Sbjct: 14 VGRNEILTWINDRLHLNLSRVEE 36 >At2g03200.1 68415.m00273 aspartyl protease family protein contains Pfam domain, PF00026: eukaryotic aspartyl protease Length = 461 Score = 30.3 bits (65), Expect = 1.5 Identities = 14/30 (46%), Positives = 18/30 (60%), Gaps = 1/30 (3%) Frame = -3 Query: 631 NGLGSSCDFGDRGDGFLSGSGPRG-GRAPV 545 +G+G C + GDGF GSG G GR P+ Sbjct: 212 SGIGFGCGVENEGDGFSQGSGLVGLGRGPL 241 >At4g30990.1 68417.m04398 expressed protein ; expression supported by MPSS Length = 2138 Score = 27.9 bits (59), Expect = 7.9 Identities = 18/66 (27%), Positives = 28/66 (42%) Frame = -1 Query: 429 HQFVNGYYLVHTHLFKTSL*YFKVLYIFMFQICLKFDSFHWYTAREQHVHELAIGGTCNN 250 H YY + F+ + K I + ICL D FH+ A++ + HE TC Sbjct: 1514 HMSWTSYYALLNRCFREMNKHTKKGKILLRLICLILDKFHF--AKDGYPHEAEEIRTCLQ 1571 Query: 249 SSIFAK 232 +F + Sbjct: 1572 KIVFPR 1577 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 17,244,466 Number of Sequences: 28952 Number of extensions: 370773 Number of successful extensions: 962 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 929 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 962 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1726528800 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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