BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00847 (807 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_37033| Best HMM Match : Annexin (HMM E-Value=0) 79 3e-15 SB_26806| Best HMM Match : Annexin (HMM E-Value=0) 65 7e-11 SB_6006| Best HMM Match : Annexin (HMM E-Value=2.4e-25) 58 1e-08 SB_7681| Best HMM Match : Annexin (HMM E-Value=0) 58 1e-08 SB_47771| Best HMM Match : Annexin (HMM E-Value=0) 48 9e-06 SB_35578| Best HMM Match : Annexin (HMM E-Value=1e-19) 47 2e-05 SB_15593| Best HMM Match : No HMM Matches (HMM E-Value=.) 38 0.013 SB_12472| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 2.5 SB_55125| Best HMM Match : zf-C2HC (HMM E-Value=1.5e-13) 29 5.9 SB_25942| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.7 SB_31195| Best HMM Match : PspA_IM30 (HMM E-Value=1) 28 7.7 SB_18204| Best HMM Match : Phage_integr_N (HMM E-Value=0.24) 28 7.7 >SB_37033| Best HMM Match : Annexin (HMM E-Value=0) Length = 287 Score = 79.4 bits (187), Expect = 3e-15 Identities = 37/84 (44%), Positives = 52/84 (61%) Frame = +2 Query: 254 HLENVIVALMTPLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGK 433 + E+ ++ALMTP + A L A+ G+GTDE +IE+L T +N I I Y L+ + Sbjct: 145 YFEDTVIALMTPPAEYDATLLRKAIKGLGTDEAVLIEVLTTRTNDEIIAIRNAYNTLFSR 204 Query: 434 SLESDLKGDTSGHFKRLCVSLCMA 505 LE D+ GDTSG FK+ +SLC A Sbjct: 205 DLEKDIAGDTSGKFKKFLISLCNA 228 Score = 44.0 bits (99), Expect = 1e-04 Identities = 19/28 (67%), Positives = 22/28 (78%) Frame = +1 Query: 583 QWGTDESIFNSILITRSYQQLRQIFAEY 666 +WGTDES FNSIL +RS+ QLR F EY Sbjct: 236 RWGTDESKFNSILASRSFDQLRATFNEY 263 Score = 42.3 bits (95), Expect = 4e-04 Identities = 23/88 (26%), Positives = 45/88 (51%) Frame = +2 Query: 338 GTDEEAIIEILCTLSNYGIRTISAFYEQLYGKSLESDLKGDTSGHFKRLCVSLCMANAMK 517 G D +A++ +LC+ +N + IS Y+ ++G+ L DLK + G+F+ ++L A Sbjct: 101 GCDNKALMYLLCSRTNSQRQRISLEYKTMFGRDLIKDLKSEVGGYFEDTVIALMTPPAEY 160 Query: 518 TRASMKAQLKPMLKHWPPLVKVNGEPTN 601 ++ +K + L++V TN Sbjct: 161 DATLLRKAIKGLGTDEAVLIEVLTTRTN 188 Score = 36.7 bits (81), Expect = 0.022 Identities = 16/57 (28%), Positives = 31/57 (54%) Frame = +1 Query: 589 GTDESIFNSILITRSYQQLRQIFAEYEALTGKDIEDSIKKEFSGSIEKGMLAIAKCV 759 GTDE++ +L TR+ ++ I Y L +D+E I + SG +K ++++ + Sbjct: 173 GTDEAVLIEVLTTRTNDEIIAIRNAYNTLFSRDLEKDIAGDTSGKFKKFLISLCNAL 229 Score = 33.5 bits (73), Expect = 0.21 Identities = 15/53 (28%), Positives = 28/53 (52%) Frame = +1 Query: 589 GTDESIFNSILITRSYQQLRQIFAEYEALTGKDIEDSIKKEFSGSIEKGMLAI 747 G D +L +R+ Q ++I EY+ + G+D+ +K E G E ++A+ Sbjct: 101 GCDNKALMYLLCSRTNSQRQRISLEYKTMFGRDLIKDLKSEVGGYFEDTVIAL 153 Score = 32.3 bits (70), Expect = 0.48 Identities = 14/22 (63%), Positives = 17/22 (77%) Frame = +1 Query: 100 LRKAMKGFGTDEKAIIDVLCRR 165 LRKA+KG GTDE +I+VL R Sbjct: 165 LRKAIKGLGTDEAVLIEVLTTR 186 Score = 30.7 bits (66), Expect = 1.5 Identities = 16/30 (53%), Positives = 21/30 (70%) Frame = +3 Query: 165 RHRASLEIAETFKTNYGKDLISELKSELTG 254 R R SLE +KT +G+DLI +LKSE+ G Sbjct: 119 RQRISLE----YKTMFGRDLIKDLKSEVGG 144 >SB_26806| Best HMM Match : Annexin (HMM E-Value=0) Length = 829 Score = 64.9 bits (151), Expect = 7e-11 Identities = 31/80 (38%), Positives = 47/80 (58%) Frame = +2 Query: 257 LENVIVALMTPLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGKS 436 + + +L+ P + L+ A+ G+GT E +IEILCT SN I+ I Y + YG+S Sbjct: 502 VHEAVTSLLLPATKADVRSLYYAMKGLGTKEAQLIEILCTRSNAEIQEIKQEYNKKYGRS 561 Query: 437 LESDLKGDTSGHFKRLCVSL 496 L D+K +TSG F+ L +L Sbjct: 562 LTDDMKSETSGDFENLLETL 581 Score = 48.4 bits (110), Expect = 7e-06 Identities = 24/101 (23%), Positives = 47/101 (46%), Gaps = 1/101 (0%) Frame = +1 Query: 448 LKRRHVGTLQEIVRVVVHGQCDENQGIDXXXXXXXXXXXXXXXXXQWGTDESIFNSILIT 627 +K G + ++ ++ G DE+ ++ +WGTDE F +IL Sbjct: 566 MKSETSGDFENLLETLLKGSRDESTDVNKELVTKDAAALVSAGVARWGTDEDQFITILSQ 625 Query: 628 RSYQQLRQIFAEYEALT-GKDIEDSIKKEFSGSIEKGMLAI 747 RS ++ + AEY+ L+ G + D+++ E G + +L + Sbjct: 626 RSSAHVQAVLAEYKTLSGGVSLADTVRDEMKGDLRDALLVL 666 Score = 40.7 bits (91), Expect = 0.001 Identities = 21/58 (36%), Positives = 27/58 (46%) Frame = +1 Query: 493 VVHGQCDENQGIDXXXXXXXXXXXXXXXXXQWGTDESIFNSILITRSYQQLRQIFAEY 666 V G+ DE+ I+ +WGTDE F I TRSY QL+ +F EY Sbjct: 155 VYQGKRDESDEINEDLAHKDAEALIEAGIKKWGTDEDCFIEIFTTRSYPQLQAMFPEY 212 Score = 37.1 bits (82), Expect = 0.017 Identities = 19/55 (34%), Positives = 29/55 (52%) Frame = +1 Query: 589 GTDESIFNSILITRSYQQLRQIFAEYEALTGKDIEDSIKKEFSGSIEKGMLAIAK 753 GT E+ IL TRS ++++I EY G+ + D +K E SG E + + K Sbjct: 529 GTKEAQLIEILCTRSNAEIQEIKQEYNKKYGRSLTDDMKSETSGDFENLLETLLK 583 Score = 36.7 bits (81), Expect = 0.022 Identities = 24/66 (36%), Positives = 40/66 (60%), Gaps = 3/66 (4%) Frame = +2 Query: 308 KELHDAVSGI---GTDEEAIIEILCTLSNYGIRTISAFYEQLYGKSLESDLKGDTSGHFK 478 ++LH+AV+ + G + +A + + N I +I Y++ YG SLE D+K +TSG F+ Sbjct: 60 EDLHEAVAAMLLPGNEADART-LYFAMKN--IDSIKEEYQKKYGCSLEDDVKEETSGDFE 116 Query: 479 RLCVSL 496 L V+L Sbjct: 117 DLLVAL 122 Score = 36.3 bits (80), Expect = 0.029 Identities = 21/68 (30%), Positives = 32/68 (47%), Gaps = 1/68 (1%) Frame = +1 Query: 511 DENQGIDXXXXXXXXXXXXXXXXXQWGTDESIFNSILITRSYQQLRQIFAEYEALT-GKD 687 DE+ ID +WGT+E F +IL RS ++ + AEY L+ G Sbjct: 760 DESTKIDEDLVTQDAAALVSAGVARWGTEEDQFITILTQRSSAHVQAVLAEYRTLSGGVT 819 Query: 688 IEDSIKKE 711 + D+I+ E Sbjct: 820 LADTIRDE 827 Score = 29.1 bits (62), Expect = 4.4 Identities = 12/23 (52%), Positives = 16/23 (69%) Frame = +1 Query: 97 TLRKAMKGFGTDEKAIIDVLCRR 165 +L AMKG GT E +I++LC R Sbjct: 520 SLYYAMKGLGTKEAQLIEILCTR 542 >SB_6006| Best HMM Match : Annexin (HMM E-Value=2.4e-25) Length = 803 Score = 57.6 bits (133), Expect = 1e-08 Identities = 29/84 (34%), Positives = 41/84 (48%) Frame = +1 Query: 502 GQCDENQGIDXXXXXXXXXXXXXXXXXQWGTDESIFNSILITRSYQQLRQIFAEYEALTG 681 GQ DE++ D WGTDE F + RS+ Q+ + AEY+ LT Sbjct: 109 GQRDESKEADIHLAKKDAKDLMEAGVASWGTDEGAFIRVFTQRSFTQISAMCAEYKKLTK 168 Query: 682 KDIEDSIKKEFSGSIEKGMLAIAK 753 +IE+SI KE G ++K L + K Sbjct: 169 VEIEESIDKEMDGDMQKAFLTLVK 192 Score = 44.0 bits (99), Expect = 1e-04 Identities = 18/29 (62%), Positives = 23/29 (79%) Frame = +2 Query: 299 FYAKELHDAVSGIGTDEEAIIEILCTLSN 385 F AK LHDA+ G+GTDE+ +IEI+CT N Sbjct: 424 FDAKCLHDAIKGLGTDEDLLIEIICTRKN 452 Score = 42.7 bits (96), Expect = 3e-04 Identities = 19/31 (61%), Positives = 23/31 (74%) Frame = +2 Query: 305 AKELHDAVSGIGTDEEAIIEILCTLSNYGIR 397 AK L++A G GTDE+A+I ILCT SN G R Sbjct: 81 AKTLYEATKGFGTDEDALISILCTRSNKGQR 111 Score = 35.9 bits (79), Expect = 0.039 Identities = 15/23 (65%), Positives = 18/23 (78%) Frame = +1 Query: 97 TLRKAMKGFGTDEKAIIDVLCRR 165 TL +A KGFGTDE A+I +LC R Sbjct: 83 TLYEATKGFGTDEDALISILCTR 105 Score = 30.3 bits (65), Expect = 1.9 Identities = 11/22 (50%), Positives = 16/22 (72%) Frame = +1 Query: 100 LRKAMKGFGTDEKAIIDVLCRR 165 L A+KG GTDE +I+++C R Sbjct: 429 LHDAIKGLGTDEDLLIEIICTR 450 >SB_7681| Best HMM Match : Annexin (HMM E-Value=0) Length = 426 Score = 57.6 bits (133), Expect = 1e-08 Identities = 28/79 (35%), Positives = 42/79 (53%) Frame = +1 Query: 502 GQCDENQGIDXXXXXXXXXXXXXXXXXQWGTDESIFNSILITRSYQQLRQIFAEYEALTG 681 G D N +D +WGTDES FN +L +RS+ QL+ F EY ++ Sbjct: 1 GNRDTNMTVDMAKATKEAQDLYKAGEKKWGTDESRFNVVLASRSFPQLQATFNEYIKISQ 60 Query: 682 KDIEDSIKKEFSGSIEKGM 738 +DI +SI +E SG ++ G+ Sbjct: 61 RDIMNSIDREMSGDLKAGL 79 Score = 47.2 bits (107), Expect = 2e-05 Identities = 22/73 (30%), Positives = 41/73 (56%) Frame = +2 Query: 299 FYAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGKSLESDLKGDTSGHFK 478 ++A L ++ G GTD+ +I ++ + S + I A + Q Y K++ ++GD SG +K Sbjct: 88 YFADRLWKSMKGAGTDDSLLIRVVVSRSEVDLVEIKASFLQKYHKTVYKMIEGDCSGDYK 147 Query: 479 RLCVSLCMANAMK 517 +L ++L N K Sbjct: 148 KLLMALPSPNTQK 160 >SB_47771| Best HMM Match : Annexin (HMM E-Value=0) Length = 529 Score = 48.0 bits (109), Expect = 9e-06 Identities = 25/66 (37%), Positives = 41/66 (62%) Frame = +2 Query: 305 AKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGKSLESDLKGDTSGHFKRL 484 A++L A+ G G+DEEAIIE+L + + I+ ++Q Y K L ++LK + SG F+ Sbjct: 308 AEKLKKAMKGFGSDEEAIIEVLQSCNLEQRLAIAQSFKQQYDKDLVNELKSELSGKFEDA 367 Query: 485 CVSLCM 502 V+L + Sbjct: 368 IVALLL 373 Score = 46.4 bits (105), Expect = 3e-05 Identities = 22/55 (40%), Positives = 32/55 (58%) Frame = +1 Query: 583 QWGTDESIFNSILITRSYQQLRQIFAEYEALTGKDIEDSIKKEFSGSIEKGMLAI 747 +WGTDES+F IL RS QLR ++ Y+ + DI ++I E +G + AI Sbjct: 417 KWGTDESVFTKILTCRSDLQLRALYQAYQHVAKCDILETIDDELTGDYHDAVKAI 471 Score = 37.9 bits (84), Expect = 0.010 Identities = 16/28 (57%), Positives = 23/28 (82%) Frame = +3 Query: 180 LEIAETFKTNYGKDLISELKSELTGTWK 263 L IA++FK Y KDL++ELKSEL+G ++ Sbjct: 338 LAIAQSFKQQYDKDLVNELKSELSGKFE 365 Score = 36.7 bits (81), Expect = 0.022 Identities = 16/45 (35%), Positives = 25/45 (55%) Frame = +2 Query: 257 LENVIVALMTPLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYG 391 + + +L+ P + L+ A+ G+GT E +IE LCT SN G Sbjct: 105 VHEAVTSLLLPATQADVRSLYYAMKGLGTKEAQLIETLCTRSNTG 149 Score = 35.5 bits (78), Expect = 0.051 Identities = 15/19 (78%), Positives = 19/19 (100%) Frame = +1 Query: 100 LRKAMKGFGTDEKAIIDVL 156 L+KAMKGFG+DE+AII+VL Sbjct: 311 LKKAMKGFGSDEEAIIEVL 329 Score = 29.9 bits (64), Expect = 2.5 Identities = 14/54 (25%), Positives = 28/54 (51%) Frame = +1 Query: 586 WGTDESIFNSILITRSYQQLRQIFAEYEALTGKDIEDSIKKEFSGSIEKGMLAI 747 +G+DE +L + + +Q I ++ KD+ + +K E SG E ++A+ Sbjct: 318 FGSDEEAIIEVLQSCNLEQRLAIAQSFKQQYDKDLVNELKSELSGKFEDAIVAL 371 >SB_35578| Best HMM Match : Annexin (HMM E-Value=1e-19) Length = 146 Score = 46.8 bits (106), Expect = 2e-05 Identities = 23/48 (47%), Positives = 31/48 (64%) Frame = +2 Query: 254 HLENVIVALMTPLPHFYAKELHDAVSGIGTDEEAIIEILCTLSNYGIR 397 +LE ++A+M P + AK L + G GTDEE +I+ILCT SN G R Sbjct: 45 NLEECLLAMMEPSVLYDAKCLRRGMRGAGTDEETLIDILCTRSNQGNR 92 Score = 40.7 bits (91), Expect = 0.001 Identities = 20/55 (36%), Positives = 26/55 (47%) Frame = +1 Query: 502 GQCDENQGIDXXXXXXXXXXXXXXXXXQWGTDESIFNSILITRSYQQLRQIFAEY 666 G D N +D +WGTDES FN +L +RS+ QL+ F EY Sbjct: 90 GNRDTNMTVDMAKATKEAQDLYKAGEKKWGTDESRFNVVLASRSFPQLQATFNEY 144 Score = 36.7 bits (81), Expect = 0.022 Identities = 15/25 (60%), Positives = 22/25 (88%) Frame = +3 Query: 180 LEIAETFKTNYGKDLISELKSELTG 254 +EI + +KT YGKDL+++LKSEL+G Sbjct: 20 VEIRKRYKTMYGKDLMNDLKSELSG 44 Score = 35.1 bits (77), Expect = 0.068 Identities = 18/52 (34%), Positives = 29/52 (55%) Frame = +2 Query: 341 TDEEAIIEILCTLSNYGIRTISAFYEQLYGKSLESDLKGDTSGHFKRLCVSL 496 T+E +I IL SN I Y+ +YGK L +DLK + SG+ + +++ Sbjct: 2 TNEAELIGILANRSNAQRVEIRKRYKTMYGKDLMNDLKSELSGNLEECLLAM 53 Score = 35.1 bits (77), Expect = 0.068 Identities = 13/22 (59%), Positives = 18/22 (81%) Frame = +1 Query: 100 LRKAMKGFGTDEKAIIDVLCRR 165 LR+ M+G GTDE+ +ID+LC R Sbjct: 65 LRRGMRGAGTDEETLIDILCTR 86 >SB_15593| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1593 Score = 37.5 bits (83), Expect = 0.013 Identities = 28/94 (29%), Positives = 49/94 (52%), Gaps = 4/94 (4%) Frame = +2 Query: 269 IVALMTPLPHFYAK-ELHDAVSGIGTDEEA---IIEILCTLSNYGIRTISAFYEQLYGKS 436 +V + +F K E+HDA+ G T+ ++ +++ L ++YG + S + +L G S Sbjct: 537 VVTVFNMTSNFTTKSEVHDAIDGAVTENDSYRKVLDFLKEWASYGNKEYSEAWIRLDGIS 596 Query: 437 LESDLKGDTSGHFKRLCVSLCMANAMKTRASMKA 538 +E+ LKG +S H R C AM RA ++ Sbjct: 597 VEA-LKGKSSWH--RFCYKDVAHTAMLKRAKTRS 627 >SB_12472| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 175 Score = 29.9 bits (64), Expect = 2.5 Identities = 12/26 (46%), Positives = 14/26 (53%) Frame = +1 Query: 292 APLLR*GAPRCCLRNWNRRRSHHRDP 369 +P L PRCC +N N R H DP Sbjct: 138 SPFLYEPGPRCCNQNMNNRSKCHADP 163 >SB_55125| Best HMM Match : zf-C2HC (HMM E-Value=1.5e-13) Length = 405 Score = 28.7 bits (61), Expect = 5.9 Identities = 14/43 (32%), Positives = 23/43 (53%), Gaps = 1/43 (2%) Frame = +3 Query: 594 RRINLQLHPYHSLLSAAETDLRRVRSFD-RKRH*GLDQERILW 719 R +NL L P H + +D ++ + D K GLD++ +LW Sbjct: 12 RDVNLTLSPAHMMAKGRNSDKQKEKDKDAEKDKDGLDRKYLLW 54 >SB_25942| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1062 Score = 28.3 bits (60), Expect = 7.7 Identities = 16/47 (34%), Positives = 23/47 (48%) Frame = +2 Query: 302 YAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGKSLE 442 Y K + + VSG GTD + + I T S ISA + K++E Sbjct: 493 YVKYMRNKVSGAGTDPDEKVFISFTASEMTSSMISAQLNSFWQKAVE 539 >SB_31195| Best HMM Match : PspA_IM30 (HMM E-Value=1) Length = 345 Score = 28.3 bits (60), Expect = 7.7 Identities = 16/47 (34%), Positives = 23/47 (48%) Frame = +2 Query: 302 YAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGKSLE 442 Y K + + VSG GTD + + I T S ISA + K++E Sbjct: 47 YVKYMRNKVSGAGTDPDEKVFISFTASEMTSSMISAQLNSFWQKAVE 93 >SB_18204| Best HMM Match : Phage_integr_N (HMM E-Value=0.24) Length = 285 Score = 28.3 bits (60), Expect = 7.7 Identities = 16/47 (34%), Positives = 23/47 (48%) Frame = +2 Query: 302 YAKELHDAVSGIGTDEEAIIEILCTLSNYGIRTISAFYEQLYGKSLE 442 Y K + + VSG GTD + + I T S ISA + K++E Sbjct: 229 YVKYMRNKVSGAGTDPDEKVFISFTASEMTSSMISAQLNSFWQKAVE 275 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 23,711,977 Number of Sequences: 59808 Number of extensions: 494008 Number of successful extensions: 1555 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 1378 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1555 length of database: 16,821,457 effective HSP length: 81 effective length of database: 11,977,009 effective search space used: 2239700683 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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