BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00822 (699 letters) Database: bee 438 sequences; 146,343 total letters Searching......................................................done Score E Sequences producing significant alignments: (bits) Value AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 297 5e-83 X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 292 2e-81 AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 186 2e-49 EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 174 6e-46 AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 31 0.008 AM076717-1|CAJ28210.1| 501|Apis mellifera serotonin receptor pr... 24 1.2 AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.2 DQ232888-1|ABB36783.1| 499|Apis mellifera cytochrome P450 monoo... 22 4.9 DQ071552-1|AAY82248.1| 495|Apis mellifera anarchy 1 protein. 22 6.4 AF134818-1|AAD40234.1| 130|Apis mellifera lambda crystallin-lik... 22 6.4 AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic ac... 21 8.5 >AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha F2 protein. Length = 461 Score = 297 bits (730), Expect = 5e-83 Identities = 148/170 (87%), Positives = 152/170 (89%), Gaps = 1/170 (0%) Frame = +2 Query: 2 EKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL 181 EK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL Sbjct: 4 EKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL 63 Query: 182 KAEREWYHNRYCSL-EVRN*QVLCYLIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEF 358 KAERE +L + + +IDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEF Sbjct: 64 KAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEF 123 Query: 359 EAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKE 508 EAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKE Sbjct: 124 EAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKE 173 Score = 133 bits (321), Expect = 2e-33 Identities = 58/64 (90%), Positives = 61/64 (95%) Frame = +1 Query: 508 ISSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALD 687 +SSYIKKIGYNPAAVAFVPISGWHGDNMLE S+KMPWFKGW VERKEGK +GKCLIEALD Sbjct: 174 VSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALD 233 Query: 688 AILP 699 AILP Sbjct: 234 AILP 237 >X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha protein. Length = 461 Score = 292 bits (716), Expect = 2e-81 Identities = 145/170 (85%), Positives = 150/170 (88%), Gaps = 1/170 (0%) Frame = +2 Query: 2 EKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL 181 EK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL Sbjct: 4 EKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL 63 Query: 182 KAEREWYHNRYCSL-EVRN*QVLCYLIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEF 358 KAERE +L + + +IDAPGHRDFIKNMITGTSQADCAVLIVAAG GEF Sbjct: 64 KAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGIGEF 123 Query: 359 EAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKE 508 EAGISKNGQTREHALLAFTLGVKQLIVGVNKMD T+PPYSE RFEEIKKE Sbjct: 124 EAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKE 173 Score = 124 bits (300), Expect = 6e-31 Identities = 54/64 (84%), Positives = 59/64 (92%) Frame = +1 Query: 508 ISSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALD 687 +SSYIKKIGYN A+VAFVPISGWHGDNMLEPS K PW+KGW+VERK+G ADGK LIEALD Sbjct: 174 VSSYIKKIGYNTASVAFVPISGWHGDNMLEPSPKTPWYKGWKVERKDGNADGKTLIEALD 233 Query: 688 AILP 699 AILP Sbjct: 234 AILP 237 >AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-alpha protein. Length = 274 Score = 186 bits (452), Expect = 2e-49 Identities = 95/116 (81%), Positives = 99/116 (85%), Gaps = 1/116 (0%) Frame = +2 Query: 164 WVLDKLKAEREWYHNRYCSL-EVRN*QVLCYLIDAPGHRDFIKNMITGTSQADCAVLIVA 340 WVLDKLKAERE +L + + +IDAPGHRDFIKNMITGTSQADCAVLIVA Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60 Query: 341 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKE 508 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKE Sbjct: 61 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKE 116 Score = 133 bits (321), Expect = 2e-33 Identities = 58/64 (90%), Positives = 61/64 (95%) Frame = +1 Query: 508 ISSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALD 687 +SSYIKKIGYNPAAVAFVPISGWHGDNMLE S+KMPWFKGW VERKEGK +GKCLIEALD Sbjct: 117 VSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALD 176 Query: 688 AILP 699 AILP Sbjct: 177 AILP 180 >EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-alpha protein. Length = 172 Score = 174 bits (424), Expect = 6e-46 Identities = 83/85 (97%), Positives = 84/85 (98%) Frame = +2 Query: 254 LIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 433 +IDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL Sbjct: 16 IIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 75 Query: 434 IVGVNKMDSTEPPYSEPRFEEIKKE 508 IVGVNKMDSTEPPYSE RFEEIKKE Sbjct: 76 IVGVNKMDSTEPPYSETRFEEIKKE 100 Score = 133 bits (321), Expect = 2e-33 Identities = 58/64 (90%), Positives = 61/64 (95%) Frame = +1 Query: 508 ISSYIKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVERKEGKADGKCLIEALD 687 +SSYIKKIGYNPAAVAFVPISGWHGDNMLE S+KMPWFKGW VERKEGK +GKCLIEALD Sbjct: 101 VSSYIKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALD 160 Query: 688 AILP 699 AILP Sbjct: 161 AILP 164 Score = 37.1 bits (82), Expect = 2e-04 Identities = 15/15 (100%), Positives = 15/15 (100%) Frame = +3 Query: 210 DIALWKFETSKYYVT 254 DIALWKFETSKYYVT Sbjct: 1 DIALWKFETSKYYVT 15 >AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation factor 2 protein. Length = 690 Score = 31.5 bits (68), Expect = 0.008 Identities = 26/82 (31%), Positives = 36/82 (43%) Frame = +2 Query: 257 IDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLI 436 +D PGH FI G D VL+VAA G E QT + +A V +I Sbjct: 198 LDTPGHAAFISMRHRGAHITDIVVLVVAADDGVKE-------QTLQSIEMAKDAKV-PII 249 Query: 437 VGVNKMDSTEPPYSEPRFEEIK 502 V +NK+D + ++E K Sbjct: 250 VAINKIDKPNIDIIKVQYELAK 271 Score = 25.4 bits (53), Expect = 0.52 Identities = 9/18 (50%), Positives = 13/18 (72%) Frame = +2 Query: 5 KTHINIVVIGHVDSGKST 58 K H + ++GHVD GK+T Sbjct: 143 KRHPIVTIMGHVDHGKTT 160 >AM076717-1|CAJ28210.1| 501|Apis mellifera serotonin receptor protein. Length = 501 Score = 24.2 bits (50), Expect = 1.2 Identities = 13/50 (26%), Positives = 20/50 (40%) Frame = -1 Query: 249 HSTC*FRTSREQYRL*YHSRSAFSLSNTQAYLKDPLPISWASFSNFSMVR 100 +STC S Q +L +H S T + + W F ++VR Sbjct: 346 NSTCSVTNSPHQKKLRFHLAKERKASTTLGIIMSAFIVCWLPFFVLALVR 395 >AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methyltransferase protein. Length = 683 Score = 24.2 bits (50), Expect = 1.2 Identities = 11/15 (73%), Positives = 11/15 (73%) Frame = +2 Query: 14 INIVVIGHVDSGKST 58 INI IGHV GKST Sbjct: 43 INIGTIGHVAHGKST 57 >DQ232888-1|ABB36783.1| 499|Apis mellifera cytochrome P450 monooxygenase protein. Length = 499 Score = 22.2 bits (45), Expect = 4.9 Identities = 11/29 (37%), Positives = 16/29 (55%) Frame = -1 Query: 201 YHSRSAFSLSNTQAYLKDPLPISWASFSN 115 Y +RS F L N +KD L ++ F+N Sbjct: 76 YATRSPFLLLNDPELIKDILIRDFSKFAN 104 >DQ071552-1|AAY82248.1| 495|Apis mellifera anarchy 1 protein. Length = 495 Score = 21.8 bits (44), Expect = 6.4 Identities = 11/36 (30%), Positives = 18/36 (50%) Frame = -2 Query: 248 IVLASFELPESNIDCDTTHAQPLVCPIPKHI*RILY 141 IV+ FE+ +S D A IP ++ R+L+ Sbjct: 54 IVIGGFEIEKSEDDSFNNQADKSEKRIPLYVCRVLH 89 >AF134818-1|AAD40234.1| 130|Apis mellifera lambda crystallin-like protein protein. Length = 130 Score = 21.8 bits (44), Expect = 6.4 Identities = 11/31 (35%), Positives = 13/31 (41%) Frame = +2 Query: 107 IEKFEKEAQEMGKGSFKYAWVLDKLKAEREW 199 + KFE E E L+KLK R W Sbjct: 84 VPKFEGEMAEKISNELNEMCPLEKLKERRIW 114 >AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic acetylcholine Apisa7-2 subunit protein. Length = 461 Score = 21.4 bits (43), Expect = 8.5 Identities = 8/24 (33%), Positives = 13/24 (54%) Frame = -1 Query: 609 FG*RLQHVVSVPSRNGHESDSSWV 538 FG L H++ V +N + + WV Sbjct: 35 FGLSLHHIIDVDEKNQILTTNCWV 58 Database: bee Posted date: Oct 23, 2007 1:17 PM Number of letters in database: 146,343 Number of sequences in database: 438 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 202,737 Number of Sequences: 438 Number of extensions: 4493 Number of successful extensions: 30 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 18 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 24 length of database: 146,343 effective HSP length: 56 effective length of database: 121,815 effective search space used: 21439440 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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