Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00816
(787 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual 48 2e-06
SPBC947.07 |||ribosome biogenesis protein Rrp14-C|Schizosaccharo... 31 0.25
SPAC19G12.13c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 27 2.3
SPAC11D3.05 |||membrane transporter|Schizosaccharomyces pombe|ch... 27 2.3
SPAC30.04c |abc4||glutathione S-conjugate-exporting ATPase Abc4|... 27 3.0
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 27 3.0
SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase Vas1|Schizos... 26 5.3
SPBC1A4.10c |pmc1|SPBP23A10.01c, med14|mediator complex subunit ... 26 7.0
SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc... 26 7.0
SPAC22F8.10c |sap145||U2 snRNP-associated protein Sap145 |Schizo... 25 9.3
>SPCC550.14 |||vigilin |Schizosaccharomyces pombe|chr 3|||Manual
Length = 1279
Score = 47.6 bits (108), Expect = 2e-06
Identities = 26/89 (29%), Positives = 46/89 (51%), Gaps = 1/89 (1%)
Frame = +3
Query: 243 QQRPVVLKGPKECIEVAKARINEIIEDLEAKVTIECVIPQRHHRTVMGARGAKVKDITAE 422
++ VVL+G KE +E + R+ EI+E+L+ +V + +PQR +++G G+ +DI +
Sbjct: 1008 ERNSVVLRGDKEIVEALETRLLEIVEELKNQVEEKIEVPQRCISSIIGRMGSTRRDIERK 1067
Query: 423 FDVQIKFPE-RDTTEGADVPSRDIDENAE 506
+ P D E + EN E
Sbjct: 1068 TSTMLNIPNVLDPEETVTITIVGSPENCE 1096
Score = 44.0 bits (99), Expect = 2e-05
Identities = 21/70 (30%), Positives = 37/70 (52%)
Frame = +3
Query: 261 LKGPKECIEVAKARINEIIEDLEAKVTIECVIPQRHHRTVMGARGAKVKDITAEFDVQIK 440
++G K+ +E ARI IE L + IP HR ++G+ G V+ + +F V+++
Sbjct: 772 IQGIKKNVEETAARIKSQIEALIDDTILRVNIPNDFHRQLIGSNGKYVRRLEEKFSVRVR 831
Query: 441 FPERDTTEGA 470
FP D + +
Sbjct: 832 FPREDDSSNS 841
Score = 40.7 bits (91), Expect = 2e-04
Identities = 16/64 (25%), Positives = 33/64 (51%)
Frame = +1
Query: 22 EDKEAIFIMGREEQVEAARKQLETAVAEISNVSEGEMSVALKHHRHFVARRGEVLRRIAE 201
E+ + + G + VE A K++ E+ N+ E + + ++HR+ + G L+ +
Sbjct: 924 EETTPVSVRGAKADVENAIKEISAIAEEVKNLVEKVIKIDREYHRYLIGPNGSKLQNTIK 983
Query: 202 DCGG 213
+CGG
Sbjct: 984 ECGG 987
Score = 33.5 bits (73), Expect = 0.035
Identities = 19/67 (28%), Positives = 32/67 (47%), Gaps = 2/67 (2%)
Frame = +3
Query: 252 PVVLKGPKECIEVAKARINEIIEDLEAKVTIECV--IPQRHHRTVMGARGAKVKDITAEF 425
P+VL G K + + E+L + TI + IP+R HR + G +G ++DI +
Sbjct: 376 PIVLSGEKSAVRECALYLQGQAEEL-LRTTIPTMLPIPRRQHRFINGEKGVGIQDILRKS 434
Query: 426 DVQIKFP 446
+ P
Sbjct: 435 GCSVILP 441
Score = 32.7 bits (71), Expect = 0.061
Identities = 17/57 (29%), Positives = 32/57 (56%)
Frame = +3
Query: 252 PVVLKGPKECIEVAKARINEIIEDLEAKVTIECVIPQRHHRTVMGARGAKVKDITAE 422
PV ++G K +E A I+ I E+++ V I + +HR ++G G+K+++ E
Sbjct: 928 PVSVRGAKADVENAIKEISAIAEEVKNLVEKVIKIDREYHRYLIGPNGSKLQNTIKE 984
Score = 32.3 bits (70), Expect = 0.081
Identities = 21/71 (29%), Positives = 32/71 (45%), Gaps = 8/71 (11%)
Frame = +2
Query: 524 IKITGRPENCEGAKKALLEQVPITID-----VEVPNELHRLLAGQKR---RELMPTYDVH 679
I+I G +N E + Q+ ID V +PN+ HR L G R L + V
Sbjct: 770 IRIQGIKKNVEETAARIKSQIEALIDDTILRVNIPNDFHRQLIGSNGKYVRRLEEKFSVR 829
Query: 680 ILLPPNEDTSD 712
+ P +D+S+
Sbjct: 830 VRFPREDDSSN 840
Score = 30.3 bits (65), Expect = 0.33
Identities = 18/77 (23%), Positives = 36/77 (46%), Gaps = 3/77 (3%)
Frame = +2
Query: 554 EGAKKALLEQVPITIDVEVPNELHRLLAGQKR---RELMPTYDVHILLPPNEDTSDIVKV 724
+G + LL T+ + +P HR + G+K ++++ ++LPP SD+V V
Sbjct: 394 QGQAEELLRTTIPTM-LPIPRRQHRFINGEKGVGIQDILRKSGCSVILPPINGDSDVVSV 452
Query: 725 TGTPTSVENAKQALLRK 775
G ++ + L +
Sbjct: 453 RGPALNISEGIRLTLER 469
Score = 30.3 bits (65), Expect = 0.33
Identities = 16/72 (22%), Positives = 39/72 (54%)
Frame = +1
Query: 37 IFIMGREEQVEAARKQLETAVAEISNVSEGEMSVALKHHRHFVARRGEVLRRIAEDCGGV 216
I I G ++ VE ++++ + + + + +++ HR + G+ +RR+ E+ V
Sbjct: 770 IRIQGIKKNVEETAARIKSQIEALIDDTILRVNIPNDFHRQLIGSNGKYVRRL-EEKFSV 828
Query: 217 QISFPRQGVNSD 252
++ FPR+ +S+
Sbjct: 829 RVRFPREDDSSN 840
Score = 29.5 bits (63), Expect = 0.57
Identities = 19/59 (32%), Positives = 31/59 (52%), Gaps = 3/59 (5%)
Frame = +2
Query: 524 IKITGRPENCEGAKKALLEQV--PITIDVEVPNELHR-LLAGQKRRELMPTYDVHILLP 691
I I G PENCE AK+ + E+V T + VP+ ++ ++ G ++L V + P
Sbjct: 1086 ITIVGSPENCEKAKEMIQEKVASQYTQMITVPDTVYESIMKGILMKKLRSDLKVFVDTP 1144
>SPBC947.07 |||ribosome biogenesis protein
Rrp14-C|Schizosaccharomyces pombe|chr 2|||Manual
Length = 233
Score = 30.7 bits (66), Expect = 0.25
Identities = 26/106 (24%), Positives = 44/106 (41%)
Frame = +3
Query: 252 PVVLKGPKECIEVAKARINEIIEDLEAKVTIECVIPQRHHRTVMGARGAKVKDITAEFDV 431
P + G + +E K RI + E+ K+ + HR ++ A G K+KD
Sbjct: 116 PTDVFGALKHLEAKKRRIESMDEEKRRKIEES----DKWHRVLLQAEGKKLKDNEQLLKK 171
Query: 432 QIKFPERDTTEGADVPSRDIDENAEPDLMT*LR*RVALKIAKELKR 569
I+ E++ + +D D + LM R LK +E K+
Sbjct: 172 SIRRKEKEKKKSSDAWKERKDNEKKAMLMRQQRREENLKKRRESKK 217
>SPAC19G12.13c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 249
Score = 27.5 bits (58), Expect = 2.3
Identities = 13/39 (33%), Positives = 25/39 (64%)
Frame = +2
Query: 8 RLKRMRIRKQYLLWVEKNKWKLPANNWKLL*QRSVMCLK 124
RL + +IRK+ L++V ++K K+P + + + R + C K
Sbjct: 187 RLSKHKIRKR-LIYVSQHKKKMPWSKFNSVLSRYIQCTK 224
>SPAC11D3.05 |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 546
Score = 27.5 bits (58), Expect = 2.3
Identities = 16/33 (48%), Positives = 21/33 (63%)
Frame = -1
Query: 343 IVTFASKSSIISLIRALATSIHSFGPLSTTGRC 245
+VTFAS S S I +A+ +HS P+ST G C
Sbjct: 118 VVTFAS-SVYSSGIIDIASELHSSIPVSTLGSC 149
>SPAC30.04c |abc4||glutathione S-conjugate-exporting ATPase
Abc4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1469
Score = 27.1 bits (57), Expect = 3.0
Identities = 20/57 (35%), Positives = 28/57 (49%), Gaps = 6/57 (10%)
Frame = -1
Query: 580 FKKSLFSSFAIFRATRYL--NYVIRSGSAFSSI----SLLGTSAPSVVSRSGNLI*T 428
F SLF+ F +F +TRYL +Y ++ + + S S V RS NLI T
Sbjct: 346 FLTSLFTQFYLFMSTRYLARSYTTLVQQIYNKVIRSRFVQNKSGDSKVGRSNNLIST 402
>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1236
Score = 27.1 bits (57), Expect = 3.0
Identities = 21/63 (33%), Positives = 33/63 (52%), Gaps = 1/63 (1%)
Frame = -3
Query: 203 SSAIRRSTSPRRATK*R*CFNATDISPSDT-LLISATAVSNCLRAASTCSSRPIINIASL 27
SS I S+ P + +++ SPS T L IS+++ S+ +AST S I + S
Sbjct: 561 SSGISSSSIPSTFSSVSSILSSSTSSPSSTSLSISSSSTSSTFSSASTSSPSSISSSISS 620
Query: 26 SSS 18
SS+
Sbjct: 621 SST 623
>SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase
Vas1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 950
Score = 26.2 bits (55), Expect = 5.3
Identities = 12/30 (40%), Positives = 20/30 (66%)
Frame = -1
Query: 571 SLFSSFAIFRATRYLNYVIRSGSAFSSISL 482
SLF++ I+RA R++N+ + SA S I +
Sbjct: 183 SLFNAGLIYRANRFVNWCPKLESAVSDIEV 212
>SPBC1A4.10c |pmc1|SPBP23A10.01c, med14|mediator complex subunit
Pmc1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 879
Score = 25.8 bits (54), Expect = 7.0
Identities = 11/31 (35%), Positives = 18/31 (58%)
Frame = -3
Query: 383 HDSSVMSLWNHTFNRNLCFQILNNFIDSCFS 291
HD + +L H FNR+ F +++ F+ FS
Sbjct: 716 HDDNTFTL--HFFNRHSPFHLISQFLQDTFS 744
>SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 587
Score = 25.8 bits (54), Expect = 7.0
Identities = 13/69 (18%), Positives = 30/69 (43%), Gaps = 3/69 (4%)
Frame = +1
Query: 19 DEDKEAIFIMGREEQVEAARKQLETAVAEISNVSEGEMSVALKHHRHFVARRGEVL---R 189
+ED + E+++ A K ++ + ++V EG+ + R G +
Sbjct: 249 EEDLHCLVTADSEDKINHAIKLIDNVIQTAASVPEGQNDLKRNQLRQLATLNGTLRDDEN 308
Query: 190 RIAEDCGGV 216
++ ++CG V
Sbjct: 309 QVCQNCGNV 317
>SPAC22F8.10c |sap145||U2 snRNP-associated protein Sap145
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 601
Score = 25.4 bits (53), Expect = 9.3
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = -2
Query: 255 PVAVDTLPRKRNLYS 211
P A DTLP+KRN +S
Sbjct: 537 PTAEDTLPQKRNAHS 551
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,225,210
Number of Sequences: 5004
Number of extensions: 67150
Number of successful extensions: 231
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 217
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 230
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 381366860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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