Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00816
(787 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_21167| Best HMM Match : KH_1 (HMM E-Value=0) 90 2e-18
SB_27288| Best HMM Match : No HMM Matches (HMM E-Value=.) 35 0.065
SB_41555| Best HMM Match : CXC (HMM E-Value=3.9) 34 0.15
SB_24937| Best HMM Match : No HMM Matches (HMM E-Value=.) 34 0.15
SB_3277| Best HMM Match : KH_1 (HMM E-Value=4.3e-30) 33 0.26
SB_54| Best HMM Match : Actin (HMM E-Value=0) 31 0.80
SB_34170| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 2.4
SB_2738| Best HMM Match : KH_1 (HMM E-Value=0.087) 29 3.2
SB_16159| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.5
SB_30536| Best HMM Match : fn3 (HMM E-Value=0.079) 28 7.5
SB_26017| Best HMM Match : Extensin_2 (HMM E-Value=0.11) 28 7.5
SB_25863| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.5
>SB_21167| Best HMM Match : KH_1 (HMM E-Value=0)
Length = 1650
Score = 89.8 bits (213), Expect = 2e-18
Identities = 41/82 (50%), Positives = 58/82 (70%)
Frame = +3
Query: 267 GPKECIEVAKARINEIIEDLEAKVTIECVIPQRHHRTVMGARGAKVKDITAEFDVQIKFP 446
G KEC+E A+ R+ EI+++LE+ VTIECVIPQ HR +MGA+GA V+++TA VQIKFP
Sbjct: 804 GAKECVEGARQRVMEIVQELESMVTIECVIPQEFHRNIMGAKGANVQEVTARHKVQIKFP 863
Query: 447 ERDTTEGADVPSRDIDENAEPD 512
+R V + D E+ +P+
Sbjct: 864 DRSPAGEEPVVNGD-GEHLDPE 884
Score = 78.2 bits (184), Expect = 7e-15
Identities = 35/97 (36%), Positives = 60/97 (61%)
Frame = +1
Query: 25 DKEAIFIMGREEQVEAARKQLETAVAEISNVSEGEMSVALKHHRHFVARRGEVLRRIAED 204
D + I I G + QVEAAR+++ E++NV++ E+ + K H + +G ++R + ED
Sbjct: 629 DSDVIIITGHKAQVEAAREKILAIQNELANVTQLEVHIPSKFHNSIIGAKGRLIRSVMED 688
Query: 205 CGGVQISFPRQGVNSDRSCLRDQKNVLK*LKHESMKL 315
CGGV I FP +G NSD+ +R K+ ++ K + ++L
Sbjct: 689 CGGVSIKFPPEGSNSDKVLIRGPKDDVEKAKKQLLEL 725
Score = 70.1 bits (164), Expect = 2e-12
Identities = 38/90 (42%), Positives = 55/90 (61%), Gaps = 3/90 (3%)
Frame = +2
Query: 518 DIIKITGRPENCEGAKKALLEQVPITIDVEVPNELHRLLAGQKR---RELMPTYDVHILL 688
DII ITG+ E+ E AK LL+ VP+T + +P + HR + G K R++M + V+I +
Sbjct: 893 DIIIITGKKESAEAAKIDLLDLVPVTEQMHIPFDYHRFVIGPKGSNVRKMMDEFSVNISI 952
Query: 689 PPNEDTSDIVKVTGTPTSVENAKQALLRKL 778
PP +D SD V V G +VE A +AL K+
Sbjct: 953 PPAKDESDSVSVIGPRANVERAMKALEAKV 982
Score = 43.2 bits (97), Expect = 2e-04
Identities = 20/65 (30%), Positives = 36/65 (55%), Gaps = 1/65 (1%)
Frame = +3
Query: 255 VVLKGPKECIEVAKARINEIIEDLE-AKVTIECVIPQRHHRTVMGARGAKVKDITAEFDV 431
V+++GPK+ +E AK ++ E+ + E T+E HHR ++G GA ++ +
Sbjct: 706 VLIRGPKDDVEKAKKQLLELTNEKELGSYTVEIRAKPEHHRFLIGRGGASIRKVRENTGA 765
Query: 432 QIKFP 446
+I FP
Sbjct: 766 RIVFP 770
Score = 41.9 bits (94), Expect = 6e-04
Identities = 17/36 (47%), Positives = 27/36 (75%)
Frame = +1
Query: 1 IFPTEKDEDKEAIFIMGREEQVEAARKQLETAVAEI 108
+FP KDEDKE I I+G++E VEAA+ +L ++ ++
Sbjct: 768 VFPAAKDEDKELITIIGKQEAVEAAKDELLKSIKDL 803
Score = 41.5 bits (93), Expect = 7e-04
Identities = 19/65 (29%), Positives = 38/65 (58%), Gaps = 1/65 (1%)
Frame = +3
Query: 327 EAKVTIECVIPQRHHRTVMGARGAKVKDITAEFDVQIKFPERDTTEGADVPSRDID-ENA 503
E +V++E I R HR ++GA+G V+ + ++ V I+FP ++ + + ++ D E A
Sbjct: 1110 EDQVSVELTIDPRIHRRLIGAKGRAVRKLMEQYKVDIRFPRQNANDPVVISGQEQDVEEA 1169
Query: 504 EPDLM 518
+ L+
Sbjct: 1170 KEQLL 1174
Score = 38.3 bits (85), Expect = 0.007
Identities = 21/65 (32%), Positives = 38/65 (58%), Gaps = 6/65 (9%)
Frame = +2
Query: 596 IDVEVPNELHRLLAGQKRR---ELMPTYDVHILLPPN---EDTSDIVKVTGTPTSVENAK 757
+DV+V + H + G+K + + YDV+I PP E+++D++ +TG S E A+
Sbjct: 999 MDVKVDRQYHPKIIGRKGQVITNIRKQYDVNIQFPPQDAPEESADVIGLTGYQHSCEAAR 1058
Query: 758 QALLR 772
A+L+
Sbjct: 1059 DAILK 1063
Score = 37.1 bits (82), Expect = 0.016
Identities = 22/90 (24%), Positives = 47/90 (52%), Gaps = 9/90 (10%)
Frame = +3
Query: 234 ARCQQRPVVLKGPKECIEVAKARINEIIEDLEAK--------VTIECVIPQRHHRTVMGA 389
A+ + V + GP+ +E A + + ++EA+ ++ + +++H ++G
Sbjct: 955 AKDESDSVSVIGPRANVERAMKALEAKVAEIEAENEDRALRSFKMDVKVDRQYHPKIIGR 1014
Query: 390 RGAKVKDITAEFDVQIKFPERDT-TEGADV 476
+G + +I ++DV I+FP +D E ADV
Sbjct: 1015 KGQVITNIRKQYDVNIQFPPQDAPEESADV 1044
Score = 35.9 bits (79), Expect = 0.037
Identities = 27/98 (27%), Positives = 46/98 (46%), Gaps = 10/98 (10%)
Frame = +2
Query: 515 NDIIKITGRPENCEGAKKALLEQV------PITIDVEVPNELHRLLAGQ---KRRELMPT 667
+D + I G ++ E AKK LLE T+++ E HR L G+ R++
Sbjct: 703 SDKVLIRGPKDDVEKAKKQLLELTNEKELGSYTVEIRAKPEHHRFLIGRGGASIRKVREN 762
Query: 668 YDVHILLPPNED-TSDIVKVTGTPTSVENAKQALLRKL 778
I+ P +D +++ + G +VE AK LL+ +
Sbjct: 763 TGARIVFPAAKDEDKELITIIGKQEAVEAAKDELLKSI 800
Score = 35.1 bits (77), Expect = 0.065
Identities = 18/70 (25%), Positives = 36/70 (51%)
Frame = +1
Query: 19 DEDKEAIFIMGREEQVEAARKQLETAVAEISNVSEGEMSVALKHHRHFVARRGEVLRRIA 198
D D E I + G ++++ A Q+ + ++V E++ HR + RRG+ +R++
Sbjct: 377 DSDSETITLRGEQDKLGVALTQV---YEKANSVVFAEVAAPRWLHRFIIGRRGQNIRKVT 433
Query: 199 EDCGGVQISF 228
+D V + F
Sbjct: 434 QDLPKVHVEF 443
Score = 34.3 bits (75), Expect = 0.11
Identities = 15/49 (30%), Positives = 27/49 (55%), Gaps = 1/49 (2%)
Frame = +3
Query: 255 VVLKGPKECIEVAKARINEIIEDLEAKVTI-ECVIPQRHHRTVMGARGA 398
+ L+GP E +E A+ + I +L + EC + Q++H ++G GA
Sbjct: 450 ITLEGPPEQVESARESLEAFIRELIVSMAFAECNVDQKYHPHIIGKNGA 498
Score = 33.5 bits (73), Expect = 0.20
Identities = 18/59 (30%), Positives = 32/59 (54%)
Frame = +3
Query: 288 VAKARINEIIEDLEAKVTIECVIPQRHHRTVMGARGAKVKDITAEFDVQIKFPERDTTE 464
VAKAR ++ L+ + IE IP+ HH+ ++G G ++ + +I P RD ++
Sbjct: 180 VAKAR-RLVLSQLQTQAQIEIQIPREHHKFILGKGGKTLQTMELSTATKITMP-RDGSD 236
Score = 31.1 bits (67), Expect = 1.1
Identities = 23/77 (29%), Positives = 35/77 (45%), Gaps = 8/77 (10%)
Frame = +2
Query: 524 IKITGRPENCEGAKKALLEQVP-----ITIDVEVPNELHRLLAGQKR---RELMPTYDVH 679
IK G E EGA++ ++E V +TI+ +P E HR + G K +E+ + V
Sbjct: 800 IKDLGAKECVEGARQRVMEIVQELESMVTIECVIPQEFHRNIMGAKGANVQEVTARHKVQ 859
Query: 680 ILLPPNEDTSDIVKVTG 730
I P + V G
Sbjct: 860 IKFPDRSPAGEEPVVNG 876
Score = 30.7 bits (66), Expect = 1.4
Identities = 17/61 (27%), Positives = 31/61 (50%), Gaps = 3/61 (4%)
Frame = +2
Query: 596 IDVEVPNELHRLLAGQKR---RELMPTYDVHILLPPNEDTSDIVKVTGTPTSVENAKQAL 766
IDV + + H+ + G+ +++ D I LP SD++ +TG VE A++ +
Sbjct: 590 IDVPIFKQFHKNVIGRGGTTIKKIREETDTKIELPAEGSDSDVIIITGHKAQVEAAREKI 649
Query: 767 L 769
L
Sbjct: 650 L 650
>SB_27288| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 458
Score = 35.1 bits (77), Expect = 0.065
Identities = 23/86 (26%), Positives = 46/86 (53%)
Frame = +3
Query: 219 NFFSSARCQQRPVVLKGPKECIEVAKARINEIIEDLEAKVTIECVIPQRHHRTVMGARGA 398
+F ++ +R +V+KG +E A+ I +I+ + ++T E +IPQ ++G GA
Sbjct: 101 DFGANRTPSERTIVIKGEREKARKAELIIKKIVAEQPRQLTEEYLIPQAACGKIIGRGGA 160
Query: 399 KVKDITAEFDVQIKFPERDTTEGADV 476
++ + + V I F + T+GA +
Sbjct: 161 TIRHL-CQVSVVI-FYRVNITQGARI 184
>SB_41555| Best HMM Match : CXC (HMM E-Value=3.9)
Length = 607
Score = 33.9 bits (74), Expect = 0.15
Identities = 15/32 (46%), Positives = 17/32 (53%)
Frame = -3
Query: 239 PCRGKEICTPPQSSAIRRSTSPRRATK*R*CF 144
PCR K++ TPP R S PRR T CF
Sbjct: 564 PCRAKKVTTPPSKRGCRHSRWPRRITCSDDCF 595
>SB_24937| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 589
Score = 33.9 bits (74), Expect = 0.15
Identities = 13/43 (30%), Positives = 25/43 (58%)
Frame = +3
Query: 336 VTIECVIPQRHHRTVMGARGAKVKDITAEFDVQIKFPERDTTE 464
V+++ I +HH ++G GA +K IT + + FP+ + T+
Sbjct: 470 VSMQIDIAPQHHLFIIGKNGANIKHITQQTGASVNFPDPNGTQ 512
>SB_3277| Best HMM Match : KH_1 (HMM E-Value=4.3e-30)
Length = 379
Score = 33.1 bits (72), Expect = 0.26
Identities = 23/84 (27%), Positives = 40/84 (47%), Gaps = 9/84 (10%)
Frame = +3
Query: 228 SSARCQQRPVVLKGPKECIEVAKARINEIIED-LEAK--------VTIECVIPQRHHRTV 380
S +R V + G K+ + A A I + +ED L++ VT+ ++P ++
Sbjct: 44 SDGSTPERIVSVTGTKDAVVTAFALIGQKLEDELKSNSKSNTTPPVTLRLIVPGSQCGSI 103
Query: 381 MGARGAKVKDITAEFDVQIKFPER 452
+G GAK+K+I + FP R
Sbjct: 104 IGKGGAKIKEIREVSGASVVFPPR 127
>SB_54| Best HMM Match : Actin (HMM E-Value=0)
Length = 2486
Score = 31.5 bits (68), Expect = 0.80
Identities = 22/86 (25%), Positives = 34/86 (39%)
Frame = +1
Query: 25 DKEAIFIMGREEQVEAARKQLETAVAEISNVSEGEMSVALKHHRHFVARRGEVLRRIAED 204
+KE + REE VEA + E A E + + H F R E +R I D
Sbjct: 1434 EKEMRLEVVREESVEATESEPEHAQQEAGERGRFDRVDGKREHYAFTVREQENMRSITSD 1493
Query: 205 CGGVQISFPRQGVNSDRSCLRDQKNV 282
+F + + S +D+K +
Sbjct: 1494 SSEEVETFGQGNLQRRESASQDEKGL 1519
>SB_34170| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 330
Score = 29.9 bits (64), Expect = 2.4
Identities = 16/44 (36%), Positives = 23/44 (52%)
Frame = -2
Query: 615 LGTSTSIVIGTCSRRAFLAPSQFSGRPVILIMSLGPVQHSHQYL 484
LG STSI++G RR P SG L+ + PV ++ + L
Sbjct: 98 LGRSTSIIVGKVKRRGDQNPGSESGLGAALLQNGQPVAYASRAL 141
>SB_2738| Best HMM Match : KH_1 (HMM E-Value=0.087)
Length = 47
Score = 29.5 bits (63), Expect = 3.2
Identities = 10/31 (32%), Positives = 21/31 (67%)
Frame = +2
Query: 680 ILLPPNEDTSDIVKVTGTPTSVENAKQALLR 772
I++P N + S+ +++ G+P VE AK +++
Sbjct: 12 IIIPSNTEKSNTIRIEGSPQGVEVAKNEIMQ 42
>SB_16159| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 351
Score = 28.3 bits (60), Expect = 7.5
Identities = 11/36 (30%), Positives = 21/36 (58%), Gaps = 2/36 (5%)
Frame = -1
Query: 754 SIFNTGW--SSCHLNNVTGIFIGWQ*DVNIICWHQF 653
S+ + GW + CH +N+ G ++G + + W+QF
Sbjct: 298 SLLSGGWWFTDCHFSNLNGEYVGDIVNEKGVVWYQF 333
>SB_30536| Best HMM Match : fn3 (HMM E-Value=0.079)
Length = 754
Score = 28.3 bits (60), Expect = 7.5
Identities = 18/61 (29%), Positives = 28/61 (45%)
Frame = +1
Query: 295 KHESMKLLRIWKQRLRLNV*FHKDITELSWVHVVPKSRTSLLNLMFKSNSLNVTLQKVQM 474
KHE +K + L V DI SW H++ S + +MF + +L+ +QM
Sbjct: 397 KHELLKHRGPLGKPLPAPVCTVMDIVTSSWKHLIDSGSNSPVAIMFWTKWCRASLEGLQM 456
Query: 475 F 477
F
Sbjct: 457 F 457
>SB_26017| Best HMM Match : Extensin_2 (HMM E-Value=0.11)
Length = 1704
Score = 28.3 bits (60), Expect = 7.5
Identities = 13/41 (31%), Positives = 22/41 (53%)
Frame = +2
Query: 560 AKKALLEQVPITIDVEVPNELHRLLAGQKRRELMPTYDVHI 682
A+KAL P++ D +P + + +G ++R PTY I
Sbjct: 445 AQKALSHITPLSPDNHIPQKTNGGFSGSQKRNQKPTYHTTI 485
>SB_25863| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 813
Score = 28.3 bits (60), Expect = 7.5
Identities = 18/64 (28%), Positives = 33/64 (51%), Gaps = 2/64 (3%)
Frame = +3
Query: 228 SSARCQ--QRPVVLKGPKECIEVAKARINEIIEDLEAKVTIECVIPQRHHRTVMGARGAK 401
S AR Q + ++G +E AK IN+++ + + + IP+ R V+G G +
Sbjct: 425 SGARIQIKEDKAYIRGTQEQKSKAKDLINKLLTSVSSGTCV--FIPEHLARHVIGKHGQR 482
Query: 402 VKDI 413
+KD+
Sbjct: 483 IKDL 486
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 24,142,943
Number of Sequences: 59808
Number of extensions: 494906
Number of successful extensions: 1720
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1485
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1719
length of database: 16,821,457
effective HSP length: 81
effective length of database: 11,977,009
effective search space used: 2155861620
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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