BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00816 (787 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g35150.1 68417.m04997 O-methyltransferase family 2 protein si... 31 0.66 At1g18370.1 68414.m02295 kinesin motor family protein (NACK1) si... 31 1.1 At4g35540.1 68417.m05051 expressed protein transcription factor ... 30 1.5 At4g29820.1 68417.m04245 expressed protein 29 2.6 At3g60910.1 68416.m06814 expressed protein low similarity to PIR... 29 2.6 At5g51650.1 68418.m06404 hypothetical protein 29 4.6 At4g00840.1 68417.m00115 zinc finger (DHHC type) family protein ... 29 4.6 At1g33680.1 68414.m04166 KH domain-containing protein similar to... 29 4.6 At5g40230.1 68418.m04881 nodulin-related low similarity to MtN21... 28 6.1 At5g35530.1 68418.m04226 40S ribosomal protein S3 (RPS3C) 28 6.1 At3g57660.1 68416.m06424 DNA-directed RNA polymerase family prot... 28 6.1 >At4g35150.1 68417.m04997 O-methyltransferase family 2 protein similar to caffeic acid O-methyltransferase, Pinus taeda, gb:U39301 Length = 325 Score = 31.5 bits (68), Expect = 0.66 Identities = 12/55 (21%), Positives = 30/55 (54%) Frame = +3 Query: 267 GPKECIEVAKARINEIIEDLEAKVTIECVIPQRHHRTVMGARGAKVKDITAEFDV 431 G K+CI++ K ++ ++ + +ECVI ++ + + R K++ + + D+ Sbjct: 225 GDKDCIKILKNCKEAVLPNIGKVLIVECVIGEKKNTMIAEERDDKLEHVRLQLDM 279 >At1g18370.1 68414.m02295 kinesin motor family protein (NACK1) similar to kinesin heavy chain isolog GB:AAB63609 GI:2262101 from [Arabidopsis thaliana] Length = 974 Score = 30.7 bits (66), Expect = 1.1 Identities = 14/40 (35%), Positives = 24/40 (60%), Gaps = 1/40 (2%) Frame = +1 Query: 43 IMGREEQVEAARKQLETAVAEI-SNVSEGEMSVALKHHRH 159 I EEQ+E+ +K ++ V + SN+S G+ + K+H H Sbjct: 582 IANLEEQLESVQKSIDKLVMSLPSNISAGDETPKTKNHHH 621 >At4g35540.1 68417.m05051 expressed protein transcription factor IIIB chain BRF1, Saccharomycescerevisiae, PIR2:A44072 Length = 527 Score = 30.3 bits (65), Expect = 1.5 Identities = 13/43 (30%), Positives = 25/43 (58%) Frame = +1 Query: 28 KEAIFIMGREEQVEAARKQLETAVAEISNVSEGEMSVALKHHR 156 K + + R E+++AA+ ++ NVSEG +S+ L+H + Sbjct: 437 KGCVAVERRREKIKAAKLRINAIQHPSDNVSEGALSLELEHSK 479 >At4g29820.1 68417.m04245 expressed protein Length = 222 Score = 29.5 bits (63), Expect = 2.6 Identities = 13/37 (35%), Positives = 19/37 (51%) Frame = +3 Query: 195 CRGLWWSTNFFSSARCQQRPVVLKGPKECIEVAKARI 305 C G+WW N F + P +K PKEC ++ R+ Sbjct: 137 CIGMWWRPN-FETLMYPFLPPNIKHPKECTKLFLVRL 172 >At3g60910.1 68416.m06814 expressed protein low similarity to PIR|I46078 endothelin converting enzyme from Bos primigenius taurus Length = 252 Score = 29.5 bits (63), Expect = 2.6 Identities = 10/27 (37%), Positives = 19/27 (70%) Frame = +2 Query: 539 RPENCEGAKKALLEQVPITIDVEVPNE 619 RPE+C + K+ +E +PIT + +P++ Sbjct: 191 RPESCSSSAKSCMEAIPITSEGMLPHD 217 >At5g51650.1 68418.m06404 hypothetical protein Length = 206 Score = 28.7 bits (61), Expect = 4.6 Identities = 16/46 (34%), Positives = 23/46 (50%) Frame = +1 Query: 22 EDKEAIFIMGREEQVEAARKQLETAVAEISNVSEGEMSVALKHHRH 159 E KE + G+EE VE + E A +S +G +SVA + H Sbjct: 40 ERKEGLEAQGKEEGVEKVLVEKEKEEAHVSWEKDGSVSVAYTYAIH 85 >At4g00840.1 68417.m00115 zinc finger (DHHC type) family protein contains Pfam profile PF01529: DHHC zinc finger domain Length = 262 Score = 28.7 bits (61), Expect = 4.6 Identities = 12/28 (42%), Positives = 17/28 (60%) Frame = +2 Query: 323 FGSKGYD*MCDSTKTSPNCHGCTWCQSQ 406 FGS GY C + K P CH C+ C+++ Sbjct: 42 FGSLGYCTKCRNVKP-PRCHHCSVCKAK 68 >At1g33680.1 68414.m04166 KH domain-containing protein similar to FUSE binding protein 2 GB:AAC50892 GI:1575607 from [Homo sapiens] Length = 759 Score = 28.7 bits (61), Expect = 4.6 Identities = 13/28 (46%), Positives = 18/28 (64%) Frame = +3 Query: 249 RPVVLKGPKECIEVAKARINEIIEDLEA 332 RPV + G CIE A+ IN +I ++EA Sbjct: 280 RPVEIIGTVSCIEKAEKLINAVIAEVEA 307 >At5g40230.1 68418.m04881 nodulin-related low similarity to MtN21 [Medicago truncatula] GI:2598575; contains Pfam profile PF00892: Integral membrane protein Length = 370 Score = 28.3 bits (60), Expect = 6.1 Identities = 14/37 (37%), Positives = 20/37 (54%) Frame = -3 Query: 290 YFNTFFWSLKHDRSLLTPCRGKEICTPPQSSAIRRST 180 +FN F +L SL+ C+G E +P +SAI T Sbjct: 85 FFNIFLLALVGFMSLIVGCKGIEYSSPTLASAISNLT 121 >At5g35530.1 68418.m04226 40S ribosomal protein S3 (RPS3C) Length = 248 Score = 28.3 bits (60), Expect = 6.1 Identities = 18/67 (26%), Positives = 36/67 (53%), Gaps = 7/67 (10%) Frame = +3 Query: 285 EVAKARINEIIED----LEAKVT---IECVIPQRHHRTVMGARGAKVKDITAEFDVQIKF 443 E+ + E+ ED +E +VT E +I + V+G +G +++++T+ + KF Sbjct: 20 ELNEVLTRELAEDGYSGVEVRVTPMRTEIIIRATRTQNVLGEKGRRIRELTSLVQKRFKF 79 Query: 444 PERDTTE 464 P+ D+ E Sbjct: 80 PQ-DSVE 85 >At3g57660.1 68416.m06424 DNA-directed RNA polymerase family protein similar to SP|O35134 DNA-directed RNA polymerase I largest subunit (EC 2.7.7.6) (RNA polymerase I 194 kDa subunit) (RPA194) {Mus musculus}; contains InterPro accession IPR000722: RNA polymerase, alpha subunit Length = 1670 Score = 28.3 bits (60), Expect = 6.1 Identities = 20/110 (18%), Positives = 50/110 (45%), Gaps = 5/110 (4%) Frame = +1 Query: 160 FVARRGEVLRRIAEDCGGVQISFPRQGVNSDRSCLRDQKNVLK*LKHESMKLLRIWKQRL 339 ++ G++ R +CG Q+ + R D+K L + +W+ + Sbjct: 1482 YIQNSGKIERCTVANCGDPQVIYHGDNPKERREISNDEKKASPALHASGVDFPALWEFQD 1541 Query: 340 RLNV--*FHKDITELSWVHVVPKSRTSL---LNLMFKSNSLNVTLQKVQM 474 +L+V + I ++ + V +R ++ +N +FKS ++V+++ + + Sbjct: 1542 KLDVRYLYSNSIHDMLNIFGVEAARETIIREINHVFKSYGISVSIRHLNL 1591 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,878,429 Number of Sequences: 28952 Number of extensions: 351906 Number of successful extensions: 1221 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 1160 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1221 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1765546400 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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