BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00803 (774 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC343.03 |apc11||anaphase-promoting complex subunit Apc11|Schi... 83 4e-17 SPAC23H4.18c |rbx1|pip1, pip1|RING-box protein 1|Schizosaccharom... 54 2e-08 SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 43 4e-05 SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo... 39 0.001 SPBC17D11.02c |||synoviolin homolog|Schizosaccharomyces pombe|ch... 38 0.002 SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 38 0.002 SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 37 0.004 SPAC3A12.03c |mug145||ubiquitin-protein ligase E3 |Schizosacchar... 35 0.015 SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces pomb... 34 0.026 SPAC17G8.10c |dma1||mitotic spindle checkpoint protein Dma1|Schi... 33 0.045 SPCC126.07c |||human CTD-binding SR-like protein rA9 homolog|Sch... 33 0.060 SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 32 0.10 SPAC6B12.07c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 31 0.14 SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 31 0.24 SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein... 30 0.42 SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 30 0.42 SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyce... 29 0.56 SPAC57A7.09 |||human RNF family homolog|Schizosaccharomyces pomb... 29 0.56 SPBC1734.06 |rhp18||Rad18 homolog Rhp18|Schizosaccharomyces pomb... 28 1.3 SPCC132.04c |||NAD-dependent glutamate dehydrogenase |Schizosacc... 27 2.3 SPCC11E10.08 |rik1||silencing protein Rik1|Schizosaccharomyces p... 27 2.3 SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 27 2.3 SPAC4F8.11 |||WD repeat protein, human WDR24 family|Schizosaccha... 27 3.9 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 27 3.9 SPAC3H1.12c |snt2||Lid2 complex subunit Snt2 |Schizosaccharomyce... 26 5.2 SPAC22F8.10c |sap145||U2 snRNP-associated protein Sap145 |Schizo... 26 6.9 SPCC162.07 |ent1||epsin|Schizosaccharomyces pombe|chr 3|||Manual 26 6.9 SPAC16C9.06c |upf1||ATP-dependent RNA helicase Upf1|Schizosaccha... 25 9.1 >SPAC343.03 |apc11||anaphase-promoting complex subunit Apc11|Schizosaccharomyces pombe|chr 1|||Manual Length = 94 Score = 83.0 bits (196), Expect = 4e-17 Identities = 32/51 (62%), Positives = 35/51 (68%), Gaps = 1/51 (1%) Frame = +2 Query: 257 MPFDSCCPDCKLPGDDCPLVWGACSHCFHIHCIVKWL-HSQPQQQCPMCRQ 406 +PFD CCP C PGD+CP+VWG C H FH HCI WL S Q QCPM RQ Sbjct: 29 VPFDGCCPQCTSPGDNCPIVWGKCKHIFHAHCIQNWLATSGSQGQCPMDRQ 79 Score = 30.7 bits (66), Expect = 0.24 Identities = 12/27 (44%), Positives = 15/27 (55%), Gaps = 1/27 (3%) Frame = +3 Query: 177 MKVTVKSWTGVATWRW-VANDDNCGIC 254 MKV + + +A W W DD CGIC Sbjct: 1 MKVKILRYHAIANWTWDTPKDDVCGIC 27 >SPAC23H4.18c |rbx1|pip1, pip1|RING-box protein 1|Schizosaccharomyces pombe|chr 1|||Manual Length = 107 Score = 54.4 bits (125), Expect = 2e-08 Identities = 20/62 (32%), Positives = 34/62 (54%) Frame = +2 Query: 233 RRQLWDLCMPFDSCCPDCKLPGDDCPLVWGACSHCFHIHCIVKWLHSQPQQQCPMCRQDW 412 R + DLC+ + +C + WG C+H FH HCI +WL++ + CP+ ++W Sbjct: 45 RNHIMDLCIECQANTDSAA--AQECTVAWGTCNHAFHFHCISRWLNT--RNVCPLDNREW 100 Query: 413 KF 418 +F Sbjct: 101 EF 102 Score = 35.5 bits (78), Expect = 0.009 Identities = 12/27 (44%), Positives = 16/27 (59%) Frame = +3 Query: 189 VKSWTGVATWRWVANDDNCGICVCHLI 269 +K W VA W+W DNC IC H++ Sbjct: 23 IKKWNAVALWQWDIVVDNCAICRNHIM 49 >SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1610 Score = 43.2 bits (97), Expect = 4e-05 Identities = 14/33 (42%), Positives = 18/33 (54%) Frame = +2 Query: 320 GACSHCFHIHCIVKWLHSQPQQQCPMCRQDWKF 418 G C H FH C+ KW S +CP+CR + F Sbjct: 1577 GTCRHKFHASCLYKWFKSSNSSRCPLCRSSFTF 1609 >SPCC18.03 |||shuttle craft like transcriptional regulator|Schizosaccharomyces pombe|chr 3|||Manual Length = 1077 Score = 38.7 bits (86), Expect = 0.001 Identities = 31/102 (30%), Positives = 47/102 (46%), Gaps = 11/102 (10%) Frame = +2 Query: 137 NKSKTSILAL*SRYESNCKKLDRGSNL-EVGSKRRQLWDLCMPFDSC--CPDCKLPGDDC 307 NKS+ S A SR N K++ +G +L ++ R + +L C C D P Sbjct: 152 NKSERSREAK-SRMPKNSKEIKKGLDLSKLDMTSRMIVELKNRLYECSVCTDTINPSTS- 209 Query: 308 PLVW--GACSHCFHIHCIVKWLHSQPQQ------QCPMCRQD 409 +W G C H FH+ CI KW + +Q +CP C+ + Sbjct: 210 --IWSCGTCYHVFHLSCIRKWCKNSIEQRNEDAWRCPYCQSN 249 >SPBC17D11.02c |||synoviolin homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 677 Score = 37.5 bits (83), Expect = 0.002 Identities = 13/27 (48%), Positives = 17/27 (62%) Frame = +2 Query: 326 CSHCFHIHCIVKWLHSQPQQQCPMCRQ 406 C H H HC+ WL + QQ CP+CR+ Sbjct: 328 CGHILHFHCLRNWL--ERQQTCPICRR 352 >SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 821 Score = 37.5 bits (83), Expect = 0.002 Identities = 20/48 (41%), Positives = 25/48 (52%), Gaps = 2/48 (4%) Frame = +2 Query: 266 DSC--CPDCKLPGDDCPLVWGACSHCFHIHCIVKWLHSQPQQQCPMCR 403 DSC C + GD C + AC H FH CI +WL + CP+CR Sbjct: 764 DSCLICLETYTNGDICRKLQ-ACKHFFHQACIDQWL-TTGNNSCPLCR 809 >SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 583 Score = 36.7 bits (81), Expect = 0.004 Identities = 14/26 (53%), Positives = 17/26 (65%) Frame = +2 Query: 326 CSHCFHIHCIVKWLHSQPQQQCPMCR 403 C+H FH CI +WL S Q CP+CR Sbjct: 544 CNHFFHRECIDQWLTSS-QNSCPLCR 568 >SPAC3A12.03c |mug145||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 309 Score = 34.7 bits (76), Expect = 0.015 Identities = 11/29 (37%), Positives = 17/29 (58%) Frame = +2 Query: 326 CSHCFHIHCIVKWLHSQPQQQCPMCRQDW 412 C H FH CI W+ + + CP+C +D+ Sbjct: 223 CEHVFHTQCIDTWMTTM-KASCPLCNEDY 250 >SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces pombe|chr 2|||Manual Length = 741 Score = 33.9 bits (74), Expect = 0.026 Identities = 19/57 (33%), Positives = 26/57 (45%) Frame = +2 Query: 239 QLWDLCMPFDSCCPDCKLPGDDCPLVWGACSHCFHIHCIVKWLHSQPQQQCPMCRQD 409 QL DL P C D D+ C H F +C+ KWL + CP+CR++ Sbjct: 101 QLMDLTCPI---CYDDMNENDEKQATKMPCGHIFGKNCLQKWLEN--HCTCPLCRKE 152 >SPAC17G8.10c |dma1||mitotic spindle checkpoint protein Dma1|Schizosaccharomyces pombe|chr 1|||Manual Length = 267 Score = 33.1 bits (72), Expect = 0.045 Identities = 23/64 (35%), Positives = 32/64 (50%), Gaps = 5/64 (7%) Frame = +2 Query: 230 KRRQLWDLCMPFDSCCPDC---KLPGDDCPLVWGA-CSHCFHIHCIVKWLH-SQPQQQCP 394 KR Q LC +S P+C +P C ++ A CSH +H CI L+ S P C Sbjct: 174 KRMQELVLCGSSESGPPECCICLMPVLPCQALFVAPCSHSYHYKCIRPTLNESHPYFSCF 233 Query: 395 MCRQ 406 +CR+ Sbjct: 234 ICRK 237 >SPCC126.07c |||human CTD-binding SR-like protein rA9 homolog|Schizosaccharomyces pombe|chr 3|||Manual Length = 571 Score = 32.7 bits (71), Expect = 0.060 Identities = 11/29 (37%), Positives = 16/29 (55%) Frame = +2 Query: 326 CSHCFHIHCIVKWLHSQPQQQCPMCRQDW 412 C H FH HC+ W + CP+CR ++ Sbjct: 56 CGHYFHNHCLESW--CRVANTCPLCRTEF 82 >SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 547 Score = 31.9 bits (69), Expect = 0.10 Identities = 14/35 (40%), Positives = 18/35 (51%) Frame = +2 Query: 311 LVWGACSHCFHIHCIVKWLHSQPQQQCPMCRQDWK 415 L+ C H FH C+ KW +S CP+CR K Sbjct: 222 LITIVCQHTFHCPCLQKWGNS----SCPVCRYTQK 252 >SPAC6B12.07c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 456 Score = 31.5 bits (68), Expect = 0.14 Identities = 12/26 (46%), Positives = 16/26 (61%) Frame = +2 Query: 326 CSHCFHIHCIVKWLHSQPQQQCPMCR 403 CSH F +HC++ L Q CP+CR Sbjct: 375 CSHVFCLHCLI-ILQKQKVDFCPLCR 399 >SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 676 Score = 30.7 bits (66), Expect = 0.24 Identities = 9/26 (34%), Positives = 17/26 (65%) Frame = +2 Query: 326 CSHCFHIHCIVKWLHSQPQQQCPMCR 403 C H +H C+++W+ + + CP+CR Sbjct: 647 CHHLYHRQCLLQWM--ETRSICPVCR 670 >SPAC19G12.16c |adg2|SPAC23A1.01c, mug46|conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 670 Score = 29.9 bits (64), Expect = 0.42 Identities = 22/59 (37%), Positives = 32/59 (54%), Gaps = 1/59 (1%) Frame = +3 Query: 333 TVSTFTVL*SGYTLSLNSSVPCADKTGSSITSETFAT*TIPGYQH-NVVARPAPSTEES 506 +VS+FT S YT +L +S TG+ S TF T + P Y + +V+ APS+ S Sbjct: 416 SVSSFTSSNSSYTTTLTASNTTVTFTGTGTGSATF-TSSPPFYSNSSVIPTSAPSSVSS 473 Score = 28.7 bits (61), Expect = 0.98 Identities = 21/59 (35%), Positives = 31/59 (52%), Gaps = 1/59 (1%) Frame = +3 Query: 333 TVSTFTVL*SGYTLSLNSSVPCADKTGSSITSETFAT*TIPGYQH-NVVARPAPSTEES 506 +VS+FT S YT +L +S TG+ S TF T + P Y + +V+ PS+ S Sbjct: 308 SVSSFTSSSSSYTTTLTASNTSVTYTGTGTGSATF-TSSPPFYSNSSVIPTSVPSSVSS 365 Score = 28.3 bits (60), Expect = 1.3 Identities = 21/59 (35%), Positives = 31/59 (52%), Gaps = 1/59 (1%) Frame = +3 Query: 333 TVSTFTVL*SGYTLSLNSSVPCADKTGSSITSETFAT*TIPGYQH-NVVARPAPSTEES 506 +VS+FT S YT +L +S TG+ S TF T + P Y + +V+ PS+ S Sbjct: 362 SVSSFTSSNSSYTTTLTASNTSITYTGTGTGSATF-TSSPPFYSNSSVIPTSVPSSVSS 419 Score = 26.6 bits (56), Expect = 3.9 Identities = 18/51 (35%), Positives = 27/51 (52%) Frame = +3 Query: 333 TVSTFTVL*SGYTLSLNSSVPCADKTGSSITSETFAT*TIPGYQHNVVARP 485 +VS+FT S YT +L +S TG+ S T AT + P Y ++ + P Sbjct: 470 SVSSFTSSNSSYTTTLTASNTTVTFTGTGTGSAT-ATSSSPYYSNSSIIVP 519 >SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 3|||Manual Length = 468 Score = 29.9 bits (64), Expect = 0.42 Identities = 10/27 (37%), Positives = 15/27 (55%) Frame = +2 Query: 326 CSHCFHIHCIVKWLHSQPQQQCPMCRQ 406 C H + C++ WL + + CP CRQ Sbjct: 100 CGHTYCYECLLNWL--KESKSCPTCRQ 124 >SPBC14F5.07 |||ER-localized ubiquitin ligase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1242 Score = 29.5 bits (63), Expect = 0.56 Identities = 16/56 (28%), Positives = 25/56 (44%), Gaps = 5/56 (8%) Frame = +2 Query: 266 DSCCPDCKLPG-DDCPLVWGA-CSHCF---HIHCIVKWLHSQPQQQCPMCRQDWKF 418 D C C+ G D PL C+ H C+V+WL + C +C+ ++F Sbjct: 5 DEICRVCRCEGAPDSPLFHPCKCTGSIRYVHQECLVEWLGHSKKTHCELCKAKFEF 60 >SPAC57A7.09 |||human RNF family homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 372 Score = 29.5 bits (63), Expect = 0.56 Identities = 11/28 (39%), Positives = 14/28 (50%) Frame = +2 Query: 326 CSHCFHIHCIVKWLHSQPQQQCPMCRQD 409 C H FH CI KW+ + CP C + Sbjct: 339 CKHEFHRPCIAKWIVDY-RHACPTCNTE 365 >SPBC1734.06 |rhp18||Rad18 homolog Rhp18|Schizosaccharomyces pombe|chr 2|||Manual Length = 387 Score = 28.3 bits (60), Expect = 1.3 Identities = 14/32 (43%), Positives = 17/32 (53%) Frame = +2 Query: 308 PLVWGACSHCFHIHCIVKWLHSQPQQQCPMCR 403 PL+ +CSH F CI +L P CP CR Sbjct: 39 PLIT-SCSHTFCSFCIRDYLREHP--MCPACR 67 >SPCC132.04c |||NAD-dependent glutamate dehydrogenase |Schizosaccharomyces pombe|chr 3|||Manual Length = 1106 Score = 27.5 bits (58), Expect = 2.3 Identities = 22/84 (26%), Positives = 36/84 (42%) Frame = +1 Query: 325 LLSLFPHSLYCKVATLSASTAVSHVQTRLEVQ*QVKHSLRKQFRAISTMSLQGLPPLPKS 504 L PH Y ++ T T V+H +++ + R + +T+ L P KS Sbjct: 3 LTKQIPH--YSRIHTTQLLTVVNHRTIPFKIRGSFSNCRR--YSNFATVPLLERPFKNKS 58 Query: 505 LMASQKTIRILRTLQTHLTKNHPP 576 + + R T + LTK+HPP Sbjct: 59 KHNTARASRPFSTQKMLLTKSHPP 82 >SPCC11E10.08 |rik1||silencing protein Rik1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1040 Score = 27.5 bits (58), Expect = 2.3 Identities = 18/63 (28%), Positives = 29/63 (46%), Gaps = 1/63 (1%) Frame = -2 Query: 773 CGGACPEVVYGWGRWWCWSESRRPFQLSPPRRHGLAQ-TPQFGQQRQIQLTQAGHFFLEI 597 C CP +VYG+ C+ LS P+ + + Q G+ I ++ G FL++ Sbjct: 613 CNNDCPHMVYGFNGALCY------MPLSMPQSYDVCQFRDNSGKDFLISVSLGGLKFLQL 666 Query: 596 NKL 588 N L Sbjct: 667 NPL 669 >SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 513 Score = 27.5 bits (58), Expect = 2.3 Identities = 11/26 (42%), Positives = 14/26 (53%) Frame = +2 Query: 326 CSHCFHIHCIVKWLHSQPQQQCPMCR 403 C H FH +CI WL + C +CR Sbjct: 414 CKHYFHENCIKPWL--RVNGTCAICR 437 >SPAC4F8.11 |||WD repeat protein, human WDR24 family|Schizosaccharomyces pombe|chr 1|||Manual Length = 846 Score = 26.6 bits (56), Expect = 3.9 Identities = 11/42 (26%), Positives = 15/42 (35%) Frame = +2 Query: 275 CPDCKLPGDDCPLVWGACSHCFHIHCIVKWLHSQPQQQCPMC 400 C C+LP + C H H C+ W P+C Sbjct: 789 CVYCELPLRGVLVYPPVCGHIGHESCLRSWYFDNTDDALPVC 830 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 26.6 bits (56), Expect = 3.9 Identities = 14/52 (26%), Positives = 25/52 (48%) Frame = +1 Query: 379 STAVSHVQTRLEVQ*QVKHSLRKQFRAISTMSLQGLPPLPKSLMASQKTIRI 534 STAV + T L + + +L K + L+G+PP P+ + + T + Sbjct: 456 STAVDNQNTVLIQVYEGERTLTKDNNLLGKFDLRGIPPAPRGVPQIEVTFEV 507 >SPAC3H1.12c |snt2||Lid2 complex subunit Snt2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1131 Score = 26.2 bits (55), Expect = 5.2 Identities = 11/29 (37%), Positives = 13/29 (44%) Frame = +2 Query: 272 CCPDCKLPGDDCPLVWGACSHCFHIHCIV 358 CC C + G + LV C C H C V Sbjct: 814 CCALCGIVGTEGLLVCFKCGTCVHERCYV 842 >SPAC22F8.10c |sap145||U2 snRNP-associated protein Sap145 |Schizosaccharomyces pombe|chr 1|||Manual Length = 601 Score = 25.8 bits (54), Expect = 6.9 Identities = 9/16 (56%), Positives = 12/16 (75%) Frame = +2 Query: 278 PDCKLPGDDCPLVWGA 325 PD K+PG +CP+ GA Sbjct: 368 PDLKIPGVNCPIPTGA 383 >SPCC162.07 |ent1||epsin|Schizosaccharomyces pombe|chr 3|||Manual Length = 706 Score = 25.8 bits (54), Expect = 6.9 Identities = 10/20 (50%), Positives = 12/20 (60%) Frame = -2 Query: 716 ESRRPFQLSPPRRHGLAQTP 657 ES PF + P+R G QTP Sbjct: 481 ESNNPFPVMQPQRTGFGQTP 500 >SPAC16C9.06c |upf1||ATP-dependent RNA helicase Upf1|Schizosaccharomyces pombe|chr 1|||Manual Length = 925 Score = 25.4 bits (53), Expect = 9.1 Identities = 12/37 (32%), Positives = 20/37 (54%) Frame = +1 Query: 475 LQGLPPLPKSLMASQKTIRILRTLQTHLTKNHPPILI 585 +QG PP + S + L T+Q+ K+H P+L+ Sbjct: 412 IQG-PPGTGKTVTSASVVYHLATMQSRKRKSHSPVLV 447 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,914,350 Number of Sequences: 5004 Number of extensions: 57454 Number of successful extensions: 163 Number of sequences better than 10.0: 28 Number of HSP's better than 10.0 without gapping: 151 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 162 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 373338084 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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