BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00796 (727 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 149 3e-37 SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 144 9e-36 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 118 9e-28 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 113 2e-26 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 110 2e-25 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 58 1e-09 SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 53 4e-08 SPBC16D10.07c |sir2||Sir2 family histone deacetylase Sir2|Schizo... 30 0.39 SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 29 0.68 SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein k... 28 1.6 SPBC646.06c |agn2||glucan endo-1,3-alpha-glucosidase Agn2|Schizo... 27 2.7 SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor Sp... 27 2.7 SPBC1703.15c |vps33|SPBC2A9.01c|vacuolar sorting protein Vps33|S... 25 8.3 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 149 bits (362), Expect = 3e-37 Identities = 77/136 (56%), Positives = 96/136 (70%) Frame = +1 Query: 103 FGNNILMCWSMAARERGDHRERPGQPYYTIVRRVHGHGASHRRRSQEPGRLNPNNTVFDA 282 F NN + + A ++G+ R P +T R+ G A ++ +NP+NT+FDA Sbjct: 19 FSNNRV---EIIANDQGN-RTTPSYVAFTDTERLIGDAAKNQVA------MNPHNTIFDA 68 Query: 283 KRLIGRKFDDPKIQQDMKHWPFKVINDCGKPKIQIEFKGETKRFAPEEISSMVLTKMKET 462 KRLIGRKFDDP++Q DMKHWPFKVI+ GKP +Q+E+KGETK F PEEISSMVL KM+ET Sbjct: 69 KRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISSMVLMKMRET 128 Query: 463 AEAYLGSTVRDAVVTV 510 AEAYLG V DAVVTV Sbjct: 129 AEAYLGGKVTDAVVTV 144 Score = 128 bits (308), Expect = 1e-30 Identities = 60/69 (86%), Positives = 66/69 (95%) Frame = +3 Query: 510 PAYFNDSQRQATKDAGAIAGLNVLRIINEPTAAALAYGLDKNLKGERNVLIFDLGGGTFD 689 PAYFNDSQRQATKDAG IAGLNVLRIINEPTAAA+AYGLD++ +GE NVLIFDLGGGTFD Sbjct: 145 PAYFNDSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQGESNVLIFDLGGGTFD 204 Query: 690 VSVLTIDEG 716 VS+LTI+EG Sbjct: 205 VSLLTIEEG 213 Score = 102 bits (245), Expect = 5e-23 Identities = 48/55 (87%), Positives = 51/55 (92%) Frame = +2 Query: 89 AIGIDLGTTYSCVGVWQHGNVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 253 +IGIDLGTTYSCVG + + VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 58 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 144 bits (350), Expect = 9e-36 Identities = 75/136 (55%), Positives = 95/136 (69%) Frame = +1 Query: 103 FGNNILMCWSMAARERGDHRERPGQPYYTIVRRVHGHGASHRRRSQEPGRLNPNNTVFDA 282 F NN + + A ++G+ R P +T R+ G A ++ +NP+NT+FDA Sbjct: 19 FSNNRV---EIIANDQGN-RTTPSYVAFTDTERLIGDAAKNQVA------MNPHNTIFDA 68 Query: 283 KRLIGRKFDDPKIQQDMKHWPFKVINDCGKPKIQIEFKGETKRFAPEEISSMVLTKMKET 462 KRLIGR+F+DP++Q DMKHWPFKVI GKP IQ+EFKGETK F PEEISSMVL KM+E+ Sbjct: 69 KRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISSMVLLKMRES 128 Query: 463 AEAYLGSTVRDAVVTV 510 AEA+LG V DAVVTV Sbjct: 129 AEAFLGGKVTDAVVTV 144 Score = 124 bits (300), Expect = 1e-29 Identities = 59/69 (85%), Positives = 65/69 (94%) Frame = +3 Query: 510 PAYFNDSQRQATKDAGAIAGLNVLRIINEPTAAALAYGLDKNLKGERNVLIFDLGGGTFD 689 PAYFNDSQRQATKDAG IAGLNVLRIINEPTAAA+AYGLD++ + E NVLIFDLGGGTFD Sbjct: 145 PAYFNDSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQHETNVLIFDLGGGTFD 204 Query: 690 VSVLTIDEG 716 VS+LTI+EG Sbjct: 205 VSLLTIEEG 213 Score = 102 bits (245), Expect = 5e-23 Identities = 48/55 (87%), Positives = 51/55 (92%) Frame = +2 Query: 89 AIGIDLGTTYSCVGVWQHGNVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 253 +IGIDLGTTYSCVG + + VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 58 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 118 bits (284), Expect = 9e-28 Identities = 52/86 (60%), Positives = 69/86 (80%) Frame = +1 Query: 253 LNPNNTVFDAKRLIGRKFDDPKIQQDMKHWPFKVINDCGKPKIQIEFKGETKRFAPEEIS 432 +NP NTVFDAKRLIGR+++DP+ Q+D+KHWPFKVI++ G P I++ + GE K+F +EIS Sbjct: 63 MNPRNTVFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEIS 122 Query: 433 SMVLTKMKETAEAYLGSTVRDAVVTV 510 +MVLTKMKE +EA L V AV+TV Sbjct: 123 AMVLTKMKEISEAKLNKRVEKAVITV 148 Score = 113 bits (271), Expect = 3e-26 Identities = 55/69 (79%), Positives = 59/69 (85%) Frame = +3 Query: 510 PAYFNDSQRQATKDAGAIAGLNVLRIINEPTAAALAYGLDKNLKGERNVLIFDLGGGTFD 689 PAYF+DSQR ATKDAGAIAGLNVLRIINEPTAAA+AYGLD +NVLIFDLGGGTFD Sbjct: 149 PAYFSDSQRAATKDAGAIAGLNVLRIINEPTAAAIAYGLDAKSDKPKNVLIFDLGGGTFD 208 Query: 690 VSVLTIDEG 716 VS+L I G Sbjct: 209 VSLLKIQGG 217 Score = 99 bits (238), Expect = 3e-22 Identities = 45/55 (81%), Positives = 49/55 (89%) Frame = +2 Query: 89 AIGIDLGTTYSCVGVWQHGNVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 253 AIGIDLGTTYSCV VW+ NVEII NDQG RTTPS+VAFT+TERL+GDAAKNQ A Sbjct: 8 AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAA 62 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 113 bits (273), Expect = 2e-26 Identities = 58/124 (46%), Positives = 83/124 (66%) Frame = +1 Query: 139 ARERGDHRERPGQPYYTIVRRVHGHGASHRRRSQEPGRLNPNNTVFDAKRLIGRKFDDPK 318 A ++G+ R P +T R+ G A ++Q P NP NT+FD KRLIGRKFD+ Sbjct: 61 ANDQGN-RITPSYVAFTEDERLVGEAA----KNQAPS--NPENTIFDIKRLIGRKFDEKT 113 Query: 319 IQQDMKHWPFKVINDCGKPKIQIEFKGETKRFAPEEISSMVLTKMKETAEAYLGSTVRDA 498 + +D+K +PF ++ND +P +++ G+ K+F PEEIS+M+L+KMK+TAEAYLG V A Sbjct: 114 MAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAMILSKMKQTAEAYLGKPVTHA 173 Query: 499 VVTV 510 VVTV Sbjct: 174 VVTV 177 Score = 113 bits (272), Expect = 2e-26 Identities = 51/69 (73%), Positives = 63/69 (91%) Frame = +3 Query: 510 PAYFNDSQRQATKDAGAIAGLNVLRIINEPTAAALAYGLDKNLKGERNVLIFDLGGGTFD 689 PAYFND+QRQATKDAG IAGLNV+RI+NEPTAAA+AYGLDK E++++++DLGGGTFD Sbjct: 178 PAYFNDAQRQATKDAGTIAGLNVIRIVNEPTAAAIAYGLDKT-DTEKHIVVYDLGGGTFD 236 Query: 690 VSVLTIDEG 716 VS+L+ID G Sbjct: 237 VSLLSIDNG 245 Score = 93.1 bits (221), Expect = 4e-20 Identities = 42/52 (80%), Positives = 47/52 (90%) Frame = +2 Query: 92 IGIDLGTTYSCVGVWQHGNVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQ 247 IGIDLGTTYSCV V ++G VEIIANDQGNR TPSYVAFT+ ERL+G+AAKNQ Sbjct: 38 IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQ 89 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 110 bits (264), Expect = 2e-25 Identities = 52/69 (75%), Positives = 60/69 (86%) Frame = +3 Query: 510 PAYFNDSQRQATKDAGAIAGLNVLRIINEPTAAALAYGLDKNLKGERNVLIFDLGGGTFD 689 PAYFNDSQRQATK AGAIAGLNVLR++NEPTAAALAYGLDK K + V +FDLGGGTFD Sbjct: 191 PAYFNDSQRQATKAAGAIAGLNVLRVVNEPTAAALAYGLDK--KNDAIVAVFDLGGGTFD 248 Query: 690 VSVLTIDEG 716 +S+L ++ G Sbjct: 249 ISILELNNG 257 Score = 83.4 bits (197), Expect = 3e-17 Identities = 38/86 (44%), Positives = 60/86 (69%) Frame = +1 Query: 253 LNPNNTVFDAKRLIGRKFDDPKIQQDMKHWPFKVINDCGKPKIQIEFKGETKRFAPEEIS 432 +NP NT F KRLIGR+F +P++Q+D+K P+K++ + +E +G+T ++P +I Sbjct: 108 INPENTFFATKRLIGRRFKEPEVQRDIKEVPYKIV-EHSNGDAWLEARGKT--YSPSQIG 164 Query: 433 SMVLTKMKETAEAYLGSTVRDAVVTV 510 +L+KM+ETA YLG V++AVVTV Sbjct: 165 GFILSKMRETASTYLGKDVKNAVVTV 190 Score = 66.5 bits (155), Expect = 4e-12 Identities = 33/57 (57%), Positives = 41/57 (71%), Gaps = 1/57 (1%) Frame = +2 Query: 80 KMPAIGIDLGTTYSCVGVWQHGNVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQ 247 K P IGIDLGTT SC+ + + ++IAN +G RTTPS VAFT D ERL+G +AK Q Sbjct: 49 KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQ 105 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 58.4 bits (135), Expect = 1e-09 Identities = 31/73 (42%), Positives = 42/73 (57%), Gaps = 4/73 (5%) Frame = +3 Query: 510 PAYFNDSQRQATKDAGAIAGLNVLRIINEPTAAALAYGLDK----NLKGERNVLIFDLGG 677 PA+F D QR+A +A IAGLN LR++N+ AAAL YG+ K + R V I D G Sbjct: 149 PAWFTDIQRRALLEAANIAGLNPLRLMNDNAAAALTYGITKTDLPEPESPRRVAIVDFGH 208 Query: 678 GTFDVSVLTIDEG 716 + VS++ G Sbjct: 209 SNYSVSIVEFSRG 221 Score = 47.6 bits (108), Expect = 2e-06 Identities = 19/54 (35%), Positives = 36/54 (66%) Frame = +2 Query: 92 IGIDLGTTYSCVGVWQHGNVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 253 +GID G + + + V ++ +++I N+ NR+TPS V++ + R +G+AAK+ A Sbjct: 8 VGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEA 61 Score = 45.2 bits (102), Expect = 1e-05 Identities = 27/86 (31%), Positives = 45/86 (52%), Gaps = 1/86 (1%) Frame = +1 Query: 256 NPNNTVFDAKRLIGRKFDDPKIQQ-DMKHWPFKVINDCGKPKIQIEFKGETKRFAPEEIS 432 N NTV KRL GR +DDP+I+ + K+ G ++++ E F+ ++ Sbjct: 63 NFRNTVGSLKRLAGRTYDDPEIKDIESNFISAKLTEVDGFVGAKVQYLNEETAFSNIQLI 122 Query: 433 SMVLTKMKETAEAYLGSTVRDAVVTV 510 + TK+K AEA L +V D V+++ Sbjct: 123 AAYFTKIKAIAEAELIGSVSDVVISI 148 >SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 53.2 bits (122), Expect = 4e-08 Identities = 26/75 (34%), Positives = 44/75 (58%) Frame = +3 Query: 492 GCGSHSPAYFNDSQRQATKDAGAIAGLNVLRIINEPTAAALAYGLDKNLKGERNVLIFDL 671 GC P YF D+QR+A + A AGL VL++I++P A LA + + ++ V++ + Sbjct: 165 GCVMSVPVYFTDAQRKALESAANEAGLPVLQLIHDPAAVILALMYSEEVLIDKTVVVANF 224 Query: 672 GGGTFDVSVLTIDEG 716 G +VSV+++ G Sbjct: 225 GATRSEVSVVSVKGG 239 Score = 37.5 bits (83), Expect = 0.002 Identities = 16/53 (30%), Positives = 29/53 (54%) Frame = +2 Query: 92 IGIDLGTTYSCVGVWQHGNVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQV 250 IGI G S + + G +++AN++GNR PS +++ + G A+ Q+ Sbjct: 27 IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQL 79 >SPBC16D10.07c |sir2||Sir2 family histone deacetylase Sir2|Schizosaccharomyces pombe|chr 2|||Manual Length = 475 Score = 29.9 bits (64), Expect = 0.39 Identities = 10/37 (27%), Positives = 22/37 (59%) Frame = +1 Query: 301 KFDDPKIQQDMKHWPFKVINDCGKPKIQIEFKGETKR 411 K D ++ +D+++ N+CGKP +++ G+ K+ Sbjct: 280 KVDGSELYEDIRNQRVSYCNECGKPPLKLRRVGQNKK 316 >SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 29.1 bits (62), Expect = 0.68 Identities = 18/57 (31%), Positives = 26/57 (45%), Gaps = 1/57 (1%) Frame = +2 Query: 77 FKMPAIGIDLGTTYSCVGVWQHG-NVEIIANDQGNRTTPSYVAFTDTERLIGDAAKN 244 F + ID GT ++ + + G +EI+ R S VAF ER+ G A N Sbjct: 20 FASSVLAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASN 76 >SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein kinase Ppk5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 836 Score = 27.9 bits (59), Expect = 1.6 Identities = 18/59 (30%), Positives = 25/59 (42%) Frame = -1 Query: 721 NDPSSMVRTDTSKVPPPRSKIRTFLSPLRFLSRPYASAAAVGSLMMRSTFNPAMAPASL 545 N PS SK PP S I ++ S P A + V + STFN ++ +L Sbjct: 335 NRPSGKKFNKNSKFRPPGSTISSYSSASTLRRLPRAPGSKVHAERQNSTFNSGISLRAL 393 >SPBC646.06c |agn2||glucan endo-1,3-alpha-glucosidase Agn2|Schizosaccharomyces pombe|chr 2|||Manual Length = 433 Score = 27.1 bits (57), Expect = 2.7 Identities = 15/46 (32%), Positives = 25/46 (54%) Frame = +3 Query: 558 AIAGLNVLRIINEPTAAALAYGLDKNLKGERNVLIFDLGGGTFDVS 695 A AGL + +I+N T A YG N+ G +V++++ T +S Sbjct: 388 AAAGLPLFQILNGTTVIAQGYG-PLNILGNNSVVLYNFNFCTTRIS 432 >SPAC1F7.01c |spt6|SPAC694.07c|transcription elongation factor Spt6|Schizosaccharomyces pombe|chr 1|||Manual Length = 1365 Score = 27.1 bits (57), Expect = 2.7 Identities = 13/32 (40%), Positives = 18/32 (56%) Frame = +3 Query: 552 AGAIAGLNVLRIINEPTAAALAYGLDKNLKGE 647 A AI +NV R+ N A L G+D N+K + Sbjct: 1049 ADAIVPVNVRRVTNRFVAVKLDCGIDGNIKAD 1080 >SPBC1703.15c |vps33|SPBC2A9.01c|vacuolar sorting protein Vps33|Schizosaccharomyces pombe|chr 2|||Manual Length = 592 Score = 25.4 bits (53), Expect = 8.3 Identities = 10/21 (47%), Positives = 15/21 (71%) Frame = +3 Query: 624 LDKNLKGERNVLIFDLGGGTF 686 L N+ G+R VL+F +GG T+ Sbjct: 528 LKTNVPGKRRVLVFFIGGCTY 548 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,857,701 Number of Sequences: 5004 Number of extensions: 58550 Number of successful extensions: 189 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 166 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 186 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 341222980 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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