Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00795
(703 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef... 149 4e-37
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef... 149 4e-37
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef... 149 4e-37
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa... 79 8e-16
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote... 76 4e-15
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E... 49 6e-07
SPAP8A3.05 |||ski complex subunit Ski7 |Schizosaccharomyces pomb... 33 0.052
SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces po... 31 0.12
SPAC23D3.08 |usp108||U1 snRNP-associated protein Usp108|Schizosa... 27 2.6
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 27 3.4
SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ... 26 4.5
SPCC1442.04c |||conserved fungal protein|Schizosaccharomyces pom... 26 6.0
SPAC688.08 |srb8|med12|mediator complex subunit Srb8 |Schizosacc... 26 6.0
SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|c... 25 7.9
SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificit... 25 7.9
>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
Length = 460
Score = 149 bits (361), Expect = 4e-37
Identities = 65/87 (74%), Positives = 75/87 (86%)
Frame = +2
Query: 251 VEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGT 430
+EP+T MPW++GWQ E K G GK L+EA+D+I PPARPTDKPLRLPLQDVYKIGGIGT
Sbjct: 200 IEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDKPLRLPLQDVYKIGGIGT 259
Query: 431 VPVGRVETGVLKPGTIVVFAPANITTK 511
VPVGRVETGV+KPG IV FAPA +TT+
Sbjct: 260 VPVGRVETGVIKPGMIVTFAPAGVTTE 286
Score = 130 bits (315), Expect = 1e-31
Identities = 72/138 (52%), Positives = 91/138 (65%), Gaps = 1/138 (0%)
Frame = +3
Query: 3 GTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYI 182
GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S+ RFEEI KE S++I
Sbjct: 119 GTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTT--GWSQARFEEIVKETSNFI 176
Query: 183 KKIGYNPAAVAFVPISGWHGDNMLSLQPKCLGSRDGRWSVKKAKLTENASLKLSMPSCHL 362
KK+G+NP V FVP+SG+ GDNM+ + G KA + + +L ++ S
Sbjct: 177 KKVGFNPKTVPFVPVSGFQGDNMIEPTTN-MPWYQGWQKETKAGVVKGKTLLEAIDSIEP 235
Query: 363 PA-PLTSPCVFPCKTYTK 413
PA P P P + K
Sbjct: 236 PARPTDKPLRLPLQDVYK 253
Score = 113 bits (272), Expect = 2e-26
Identities = 50/64 (78%), Positives = 56/64 (87%)
Frame = +1
Query: 511 VKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLN 690
VKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LN
Sbjct: 287 VKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILN 346
Query: 691 HPGQ 702
HPGQ
Sbjct: 347 HPGQ 350
>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
Length = 460
Score = 149 bits (361), Expect = 4e-37
Identities = 65/87 (74%), Positives = 75/87 (86%)
Frame = +2
Query: 251 VEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGT 430
+EP+T MPW++GWQ E K G GK L+EA+D+I PPARPTDKPLRLPLQDVYKIGGIGT
Sbjct: 200 IEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDKPLRLPLQDVYKIGGIGT 259
Query: 431 VPVGRVETGVLKPGTIVVFAPANITTK 511
VPVGRVETGV+KPG IV FAPA +TT+
Sbjct: 260 VPVGRVETGVIKPGMIVTFAPAGVTTE 286
Score = 130 bits (315), Expect = 1e-31
Identities = 72/138 (52%), Positives = 91/138 (65%), Gaps = 1/138 (0%)
Frame = +3
Query: 3 GTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYI 182
GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S+ RFEEI KE S++I
Sbjct: 119 GTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTT--GWSQARFEEIVKETSNFI 176
Query: 183 KKIGYNPAAVAFVPISGWHGDNMLSLQPKCLGSRDGRWSVKKAKLTENASLKLSMPSCHL 362
KK+G+NP V FVP+SG+ GDNM+ + G KA + + +L ++ S
Sbjct: 177 KKVGFNPKTVPFVPVSGFQGDNMIEPTTN-MPWYQGWQKETKAGVVKGKTLLEAIDSIEP 235
Query: 363 PA-PLTSPCVFPCKTYTK 413
PA P P P + K
Sbjct: 236 PARPTDKPLRLPLQDVYK 253
Score = 113 bits (272), Expect = 2e-26
Identities = 50/64 (78%), Positives = 56/64 (87%)
Frame = +1
Query: 511 VKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLN 690
VKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LN
Sbjct: 287 VKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILN 346
Query: 691 HPGQ 702
HPGQ
Sbjct: 347 HPGQ 350
>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 149 bits (361), Expect = 4e-37
Identities = 65/87 (74%), Positives = 75/87 (86%)
Frame = +2
Query: 251 VEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGT 430
+EP+T MPW++GWQ E K G GK L+EA+D+I PPARPTDKPLRLPLQDVYKIGGIGT
Sbjct: 200 IEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDKPLRLPLQDVYKIGGIGT 259
Query: 431 VPVGRVETGVLKPGTIVVFAPANITTK 511
VPVGRVETGV+KPG IV FAPA +TT+
Sbjct: 260 VPVGRVETGVIKPGMIVTFAPAGVTTE 286
Score = 130 bits (315), Expect = 1e-31
Identities = 72/138 (52%), Positives = 91/138 (65%), Gaps = 1/138 (0%)
Frame = +3
Query: 3 GTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYI 182
GTGEFEAGISK+GQTREHALLA+TLGVKQLIV VNKMD+T +S+ RFEEI KE S++I
Sbjct: 119 GTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTT--GWSQARFEEIVKETSNFI 176
Query: 183 KKIGYNPAAVAFVPISGWHGDNMLSLQPKCLGSRDGRWSVKKAKLTENASLKLSMPSCHL 362
KK+G+NP V FVP+SG+ GDNM+ + G KA + + +L ++ S
Sbjct: 177 KKVGFNPKTVPFVPVSGFQGDNMIEPTTN-MPWYQGWQKETKAGVVKGKTLLEAIDSIEP 235
Query: 363 PA-PLTSPCVFPCKTYTK 413
PA P P P + K
Sbjct: 236 PARPTDKPLRLPLQDVYK 253
Score = 113 bits (272), Expect = 2e-26
Identities = 50/64 (78%), Positives = 56/64 (87%)
Frame = +1
Query: 511 VKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLN 690
VKSVEMHHE+L +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LN
Sbjct: 287 VKSVEMHHESLDAGLPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILN 346
Query: 691 HPGQ 702
HPGQ
Sbjct: 347 HPGQ 350
>SPCC584.04 |sup35|erf3|translation release factor eRF3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 78.6 bits (185), Expect = 8e-16
Identities = 36/83 (43%), Positives = 56/83 (67%), Gaps = 2/83 (2%)
Frame = +3
Query: 9 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 188
GEFEAG + GQTREHA+LA T G+ L+V +NKMD +SE R++E ++S ++++
Sbjct: 352 GEFEAGFERGGQTREHAVLARTQGINHLVVVINKMDEPSVQWSEERYKECVDKLSMFLRR 411
Query: 189 I-GYNPAA-VAFVPISGWHGDNM 251
+ GYN V ++P+S + G N+
Sbjct: 412 VAGYNSKTDVKYMPVSAYTGQNV 434
Score = 37.9 bits (84), Expect = 0.001
Identities = 20/62 (32%), Positives = 33/62 (53%)
Frame = +2
Query: 320 GKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGTIVVFAPAN 499
G L+E LD++ R + P +P+ YK +GT+ G++E G +K + V+ P N
Sbjct: 448 GPSLLEYLDSMTHLERKVNAPFIMPIASKYK--DLGTILEGKIEAGSIKKNSNVLVMPIN 505
Query: 500 IT 505
T
Sbjct: 506 QT 507
>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 592
Score = 76.2 bits (179), Expect = 4e-15
Identities = 39/81 (48%), Positives = 53/81 (65%), Gaps = 1/81 (1%)
Frame = +3
Query: 15 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSY-IKKI 191
FE G +NGQTREHA L LG+ +++V VNK+D +SE RF+EIK VS + IK +
Sbjct: 293 FERGFLENGQTREHAYLLRALGISEIVVSVNKLDLMS--WSEDRFQEIKNIVSDFLIKMV 350
Query: 192 GYNPAAVAFVPISGWHGDNML 254
G+ + V FVPIS G N++
Sbjct: 351 GFKTSNVHFVPISAISGTNLI 371
Score = 45.2 bits (102), Expect = 9e-06
Identities = 23/46 (50%), Positives = 29/46 (63%)
Frame = +2
Query: 320 GKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGTVPVGRVETG 457
G L+ ALD ++PP +P KPLRL + DVY+ TV GRVE G
Sbjct: 384 GPTLLSALDQLVPPEKPYRKPLRLSIDDVYRSPRSVTV-TGRVEAG 428
>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 439
Score = 49.2 bits (112), Expect = 6e-07
Identities = 24/49 (48%), Positives = 32/49 (65%), Gaps = 1/49 (2%)
Frame = +2
Query: 329 LIEALDAILP-PARPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPG 472
L+EA+D+ + P R TD P + ++DV+ I G GTV GRVE G LK G
Sbjct: 234 LMEAVDSYITLPERKTDVPFLMAIEDVFSISGRGTVVTGRVERGTLKKG 282
Score = 45.2 bits (102), Expect = 9e-06
Identities = 22/46 (47%), Positives = 31/46 (67%)
Frame = +3
Query: 42 QTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSY 179
QTREH LLA +GVKQ++V +NK+D EP E E+++ +S Y
Sbjct: 156 QTREHLLLARQVGVKQIVVYINKVDMVEPDMIELVEMEMRELLSEY 201
Score = 32.3 bits (70), Expect = 0.069
Identities = 13/37 (35%), Positives = 23/37 (62%)
Frame = +1
Query: 511 VKSVEMHHEALQEAVPGDNVGFNVKNVSVKELRRGYV 621
V +EM + L AV GDN G ++++ ++L+RG +
Sbjct: 298 VTGIEMFKKQLDAAVAGDNCGLLLRSIKREQLKRGMI 334
>SPAP8A3.05 |||ski complex subunit Ski7 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 695
Score = 32.7 bits (71), Expect = 0.052
Identities = 18/67 (26%), Positives = 32/67 (47%)
Frame = +3
Query: 75 LGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNML 254
L + + + KMD E + E +F + + S++K+ F+PISG G N+
Sbjct: 407 LSISSYMFAITKMDEIE--WDENKFINLVNSIQSFLKESCGIIEKSKFIPISGLKGTNLT 464
Query: 255 SLQPKCL 275
S+ + L
Sbjct: 465 SISQEKL 471
>SPAC1B3.04c |||mitochondrial GTPase Guf1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 646
Score = 31.5 bits (68), Expect = 0.12
Identities = 22/74 (29%), Positives = 33/74 (44%)
Frame = +2
Query: 251 VEPSTKMPWFKGWQVERKEGKADGKCLIEALDAILPPARPTDKPLRLPLQDVYKIGGIGT 430
V+ + +P K V K GK + L E + I PP + PLR L D + G
Sbjct: 200 VQQTFDIPMSKPILVSSKTGKNVEQILPEIIQKIPPPKGSENAPLRCLLIDSWYNSYQGV 259
Query: 431 VPVGRVETGVLKPG 472
+ + R+ G +K G
Sbjct: 260 IGLVRIMEGFIKKG 273
>SPAC23D3.08 |usp108||U1 snRNP-associated protein
Usp108|Schizosaccharomyces pombe|chr 1|||Manual
Length = 382
Score = 27.1 bits (57), Expect = 2.6
Identities = 23/76 (30%), Positives = 33/76 (43%), Gaps = 3/76 (3%)
Frame = -2
Query: 609 TQFLDGHVLYVET---YIVSRYSFLESFVVHLHRFDLVVMLAGAKTTMVPGFNTPVSTLP 439
T +D V+ +ET YI+SR SF+ V + D + G+ +T + P
Sbjct: 162 TNEIDASVV-IETLLKYILSRISFIPRVSVVPYIPDAAYGIYGSPSTFTQFNHMSSMVTP 220
Query: 438 TGTVPIPPILYTSCRG 391
IPPI TS G
Sbjct: 221 ENPYGIPPIALTSAGG 236
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 26.6 bits (56), Expect = 3.4
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = -2
Query: 606 QFLDGHVLYVETYIVSRYSFLESFV 532
+F DGH+L ETY+ LES V
Sbjct: 527 KFFDGHLLASETYVGGHVESLESGV 551
>SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 26.2 bits (55), Expect = 4.5
Identities = 22/72 (30%), Positives = 25/72 (34%), Gaps = 5/72 (6%)
Frame = -2
Query: 471 PGFNTPVSTLPTGTVPIP-PILYTSCR--GRRRGLSVGRAGGRMASRASMRH--FPSALP 307
P +N P G+VP+P P S R R S GR S H PS LP
Sbjct: 314 PNYNAPFQPAGVGSVPLPAPTSSQSLRLGSLHRSRSPSPRSGRPRRSPSPSHLSIPSTLP 373
Query: 306 SLRSTCHPLNQG 271
P G
Sbjct: 374 PADGVPKPTPDG 385
>SPCC1442.04c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 409
Score = 25.8 bits (54), Expect = 6.0
Identities = 16/56 (28%), Positives = 27/56 (48%), Gaps = 1/56 (1%)
Frame = -1
Query: 571 LHCLQVQLLGELRGASPQI*LSSDVGGGKDNNGTW-FQHTSFNSADGHGTNTTDFV 407
LH +V+ + E SP + SDV K+N T+ + +FN + N T+ +
Sbjct: 42 LHDGKVEEVVEAINGSPDLLSESDVENAKNNEHTYDIDNKNFNFEPKNSVNKTETI 97
>SPAC688.08 |srb8|med12|mediator complex subunit Srb8
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1233
Score = 25.8 bits (54), Expect = 6.0
Identities = 10/39 (25%), Positives = 22/39 (56%)
Frame = -2
Query: 630 VTSNITTTQFLDGHVLYVETYIVSRYSFLESFVVHLHRF 514
+T ++ + L H+ +++ SR +FL ++H+H F
Sbjct: 1012 LTQSLQSFSHLSNHIEVLDSTRQSRLTFLCHLILHMHGF 1050
>SPBC24C6.08c |||vesicle coat protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 367
Score = 25.4 bits (53), Expect = 7.9
Identities = 9/30 (30%), Positives = 18/30 (60%)
Frame = +1
Query: 133 TVSPDLRKSRRKYPHTSRRLATTQLLSLSC 222
+ +PD+ ++PHT +R +T + L + C
Sbjct: 145 STNPDVLYMSSQHPHTQQRYSTLKRLMVRC 174
>SPAC6F12.17 |rna14||mRNA cleavage and polyadenylation specificity
factor complex subunit Rna14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 733
Score = 25.4 bits (53), Expect = 7.9
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -2
Query: 462 NTPVSTLPTGTVPIPPIL 409
N P S LPT VP+P I+
Sbjct: 666 NPPTSALPTVPVPLPSII 683
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,240,620
Number of Sequences: 5004
Number of extensions: 72503
Number of successful extensions: 247
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 221
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 242
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 325165428
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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