BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00778 (700 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g20980.1 68418.m02494 5-methyltetrahydropteroyltriglutamate--... 29 2.2 At2g47410.1 68415.m05917 transducin family protein / WD-40 repea... 29 3.0 At5g20380.1 68418.m02424 transporter-related low similarity to v... 29 3.9 At2g20880.1 68415.m02461 AP2 domain-containing transcription fac... 29 3.9 At5g24350.1 68418.m02870 expressed protein weak similarity to ne... 28 5.2 At5g03280.1 68418.m00277 ethylene-insensitive 2 (EIN2) identical... 28 5.2 At4g14300.1 68417.m02203 heterogeneous nuclear ribonucleoprotein... 28 5.2 At3g03780.2 68416.m00387 5-methyltetrahydropteroyltriglutamate--... 27 9.0 At3g03780.1 68416.m00386 5-methyltetrahydropteroyltriglutamate--... 27 9.0 At1g20110.1 68414.m02516 zinc finger (FYVE type) family protein ... 27 9.0 >At5g20980.1 68418.m02494 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase, putative / vitamin-B12-independent methionine synthase, putative / cobalamin-independent methionine synthase, putative strong similarity to SP|O50008 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC 2.1.1.14) (Vitamin-B12-independent methionine synthase isozyme) (Cobalamin-independent methionine synthase isozyme) {Arabidopsis thaliana}; contains Pfam profile PF01717: Methionine synthase, vitamin-B12 independent Length = 812 Score = 29.5 bits (63), Expect = 2.2 Identities = 10/38 (26%), Positives = 19/38 (50%) Frame = +1 Query: 469 VWDHLHRQCLVWVPPSTYARYGTTSTANAWYGSAPSTY 582 +W H+ + ++P +T++ Y A G+ PS Y Sbjct: 93 IWKHMAHAGIKYIPSNTFSYYDQMLDTTAMLGAVPSRY 130 >At2g47410.1 68415.m05917 transducin family protein / WD-40 repeat family protein contains 5 WD-40 repeats (PF00400); similar to WDR protein, form B (GI:14970593) [Mus musculus] Length = 1589 Score = 29.1 bits (62), Expect = 3.0 Identities = 16/57 (28%), Positives = 27/57 (47%), Gaps = 2/57 (3%) Frame = +1 Query: 463 CQVWDHLHRQCL--VWVPPSTYARYGTTSTANAWYGSAPSTYARYGTASTANARDGS 627 C++WD + Q L ++VP + A G+TS A+ + Y GT + D + Sbjct: 416 CRIWDARYSQWLPRIYVPSPSDANTGSTSNASQSHQILCCAYNANGTIFVTGSSDSN 472 >At5g20380.1 68418.m02424 transporter-related low similarity to vesicular glutamate transporter 3 [Rattus norvegicus] GI:21685382 Length = 517 Score = 28.7 bits (61), Expect = 3.9 Identities = 15/55 (27%), Positives = 19/55 (34%), Gaps = 1/55 (1%) Frame = +1 Query: 412 WDHLHRQCLVW-VLPLHLCQVWDHLHRQCLVWVPPSTYARYGTTSTANAWYGSAP 573 W + VW ++ H C W H CL W+P T AW P Sbjct: 310 WKSFFQSPAVWAMIYTHFCGSWGHY--TCLSWLPTYFSEALSLNLTEAAWVSILP 362 >At2g20880.1 68415.m02461 AP2 domain-containing transcription factor, putative similar to AP2 domain containing protein RAP2.4 [Arabidopsis thaliana] GI:2281633 Length = 336 Score = 28.7 bits (61), Expect = 3.9 Identities = 14/43 (32%), Positives = 19/43 (44%) Frame = +1 Query: 565 SAPSTYARYGTASTANARDGSTPSTYARHNPYATCNARCTPPP 693 S P + G AS+ + R T+ RHNPY + PP Sbjct: 41 SGPGSDMWLGDASSTSPRSLRKTRTFDRHNPYLVSSYATPQPP 83 >At5g24350.1 68418.m02870 expressed protein weak similarity to neuroblastoma-amplified protein [Homo sapiens] GI:4337460 Length = 2376 Score = 28.3 bits (60), Expect = 5.2 Identities = 12/41 (29%), Positives = 24/41 (58%) Frame = +1 Query: 355 EHLRRQCLVWVRPLHLCQIWDHLHRQCLVWVLPLHLCQVWD 477 E ++++C+V PLH C W + R+ + +P ++ Q+ D Sbjct: 2104 EKVKKECVVSAHPLHSC--WLDIFRKYIALSMPENVLQLID 2142 >At5g03280.1 68418.m00277 ethylene-insensitive 2 (EIN2) identical to EIN2 [Arabidopsis thaliana] gi|5231113|gb|AAD41076; member of the natural resistance-associated macrophage protein (NRAMP) metal transporter family, PMID:11500563; metal transport capacity has not been shown, PMID:11500563, PMID:1038174 Length = 1294 Score = 28.3 bits (60), Expect = 5.2 Identities = 13/41 (31%), Positives = 19/41 (46%) Frame = +2 Query: 290 YGTTTTPNAWYWSAPSTYARYRSTSAANAWYGSAPSTYARY 412 YG TT N Y + Y+ R+ S++ W P+T Y Sbjct: 783 YGNTTNNNNAYELSERRYSSLRAPSSSEGWEHQQPATVHGY 823 >At4g14300.1 68417.m02203 heterogeneous nuclear ribonucleoprotein, putative / hnRNP, putative Length = 411 Score = 28.3 bits (60), Expect = 5.2 Identities = 17/56 (30%), Positives = 26/56 (46%) Frame = +2 Query: 341 YARYRSTSAANAWYGSAPSTYARYGTTSTANA*YGSSPSTYARYGTTSTANA*YGS 508 Y Y + + YG+ + A YG T+ A YGSS R + A++ YG+ Sbjct: 257 YGAYGGYTGSAGGYGAGAT--AGYGATNIPGAGYGSSTGVAPRNSWDTPASSGYGN 310 Score = 28.3 bits (60), Expect = 5.2 Identities = 24/83 (28%), Positives = 38/83 (45%), Gaps = 8/83 (9%) Frame = +2 Query: 284 ARYGTTTTPNAWYWSAPSTYARYRSTSAANAWYGS----APSTYARYGTTSTANA*YGSS 451 A YG T P A Y S+ R + A++ YG+ + + ++ YG A S Sbjct: 276 AGYGATNIPGAGYGSSTGVAPRNSWDTPASSGYGNPGYGSGAAHSGYGVPGAAPP--TQS 333 Query: 452 PSTYAR----YGTTSTANA*YGS 508 PS Y+ YG S +++ YG+ Sbjct: 334 PSGYSNQGYGYGGYSGSDSGYGN 356 >At3g03780.2 68416.m00387 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase, putative / vitamin-B12-independent methionine synthase, putative / cobalamin-independent methionine synthase, putative very strong similarity to SP|O50008 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC 2.1.1.14) (Vitamin-B12-independent methionine synthase isozyme) (Cobalamin-independent methionine synthase isozyme) {Arabidopsis thaliana}; contains Pfam profile PF01717: Methionine synthase, vitamin-B12 independent Length = 765 Score = 27.5 bits (58), Expect = 9.0 Identities = 9/38 (23%), Positives = 18/38 (47%) Frame = +1 Query: 469 VWDHLHRQCLVWVPPSTYARYGTTSTANAWYGSAPSTY 582 +W + + ++P +T++ Y A G+ PS Y Sbjct: 45 IWKQMSAAGIKYIPSNTFSHYDQVLDTTAMLGAVPSRY 82 >At3g03780.1 68416.m00386 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase, putative / vitamin-B12-independent methionine synthase, putative / cobalamin-independent methionine synthase, putative very strong similarity to SP|O50008 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC 2.1.1.14) (Vitamin-B12-independent methionine synthase isozyme) (Cobalamin-independent methionine synthase isozyme) {Arabidopsis thaliana}; contains Pfam profile PF01717: Methionine synthase, vitamin-B12 independent Length = 765 Score = 27.5 bits (58), Expect = 9.0 Identities = 9/38 (23%), Positives = 18/38 (47%) Frame = +1 Query: 469 VWDHLHRQCLVWVPPSTYARYGTTSTANAWYGSAPSTY 582 +W + + ++P +T++ Y A G+ PS Y Sbjct: 45 IWKQMSAAGIKYIPSNTFSHYDQVLDTTAMLGAVPSRY 82 >At1g20110.1 68414.m02516 zinc finger (FYVE type) family protein contains Pfam profile: PF01363 FYVE zinc finger Length = 601 Score = 27.5 bits (58), Expect = 9.0 Identities = 19/65 (29%), Positives = 26/65 (40%), Gaps = 3/65 (4%) Frame = +1 Query: 508 PPSTYARYGTTSTANAWYGSAPSTYARYGTASTANARDGSTP-STYARHNPYA--TCNAR 678 PP+ G T Y SAP YG+A +N TP + H P + + + Sbjct: 32 PPAPATVAGPTDLTRNTYASAPPFTGGYGSADYSNYSQNYTPYGQNSEHVPPSAPSFTSP 91 Query: 679 CTPPP 693 PPP Sbjct: 92 SQPPP 96 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,911,589 Number of Sequences: 28952 Number of extensions: 258093 Number of successful extensions: 677 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 595 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 675 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1496852856 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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