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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00778
         (700 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g20980.1 68418.m02494 5-methyltetrahydropteroyltriglutamate--...    29   2.2  
At2g47410.1 68415.m05917 transducin family protein / WD-40 repea...    29   3.0  
At5g20380.1 68418.m02424 transporter-related low similarity to v...    29   3.9  
At2g20880.1 68415.m02461 AP2 domain-containing transcription fac...    29   3.9  
At5g24350.1 68418.m02870 expressed protein weak similarity to ne...    28   5.2  
At5g03280.1 68418.m00277 ethylene-insensitive 2 (EIN2) identical...    28   5.2  
At4g14300.1 68417.m02203 heterogeneous nuclear ribonucleoprotein...    28   5.2  
At3g03780.2 68416.m00387 5-methyltetrahydropteroyltriglutamate--...    27   9.0  
At3g03780.1 68416.m00386 5-methyltetrahydropteroyltriglutamate--...    27   9.0  
At1g20110.1 68414.m02516 zinc finger (FYVE type) family protein ...    27   9.0  

>At5g20980.1 68418.m02494
           5-methyltetrahydropteroyltriglutamate--homocysteine
           methyltransferase, putative / vitamin-B12-independent
           methionine synthase, putative / cobalamin-independent
           methionine synthase, putative strong similarity to
           SP|O50008
           5-methyltetrahydropteroyltriglutamate--homocysteine
           methyltransferase (EC 2.1.1.14) (Vitamin-B12-independent
           methionine synthase isozyme) (Cobalamin-independent
           methionine synthase isozyme) {Arabidopsis thaliana};
           contains Pfam profile PF01717: Methionine synthase,
           vitamin-B12 independent
          Length = 812

 Score = 29.5 bits (63), Expect = 2.2
 Identities = 10/38 (26%), Positives = 19/38 (50%)
 Frame = +1

Query: 469 VWDHLHRQCLVWVPPSTYARYGTTSTANAWYGSAPSTY 582
           +W H+    + ++P +T++ Y       A  G+ PS Y
Sbjct: 93  IWKHMAHAGIKYIPSNTFSYYDQMLDTTAMLGAVPSRY 130


>At2g47410.1 68415.m05917 transducin family protein / WD-40 repeat
           family protein contains 5 WD-40 repeats (PF00400);
           similar to WDR protein, form B (GI:14970593) [Mus
           musculus]
          Length = 1589

 Score = 29.1 bits (62), Expect = 3.0
 Identities = 16/57 (28%), Positives = 27/57 (47%), Gaps = 2/57 (3%)
 Frame = +1

Query: 463 CQVWDHLHRQCL--VWVPPSTYARYGTTSTANAWYGSAPSTYARYGTASTANARDGS 627
           C++WD  + Q L  ++VP  + A  G+TS A+  +      Y   GT     + D +
Sbjct: 416 CRIWDARYSQWLPRIYVPSPSDANTGSTSNASQSHQILCCAYNANGTIFVTGSSDSN 472


>At5g20380.1 68418.m02424 transporter-related low similarity to
           vesicular glutamate transporter 3 [Rattus norvegicus]
           GI:21685382
          Length = 517

 Score = 28.7 bits (61), Expect = 3.9
 Identities = 15/55 (27%), Positives = 19/55 (34%), Gaps = 1/55 (1%)
 Frame = +1

Query: 412 WDHLHRQCLVW-VLPLHLCQVWDHLHRQCLVWVPPSTYARYGTTSTANAWYGSAP 573
           W    +   VW ++  H C  W H    CL W+P           T  AW    P
Sbjct: 310 WKSFFQSPAVWAMIYTHFCGSWGHY--TCLSWLPTYFSEALSLNLTEAAWVSILP 362


>At2g20880.1 68415.m02461 AP2 domain-containing transcription
           factor, putative similar to AP2 domain containing
           protein RAP2.4 [Arabidopsis thaliana] GI:2281633
          Length = 336

 Score = 28.7 bits (61), Expect = 3.9
 Identities = 14/43 (32%), Positives = 19/43 (44%)
 Frame = +1

Query: 565 SAPSTYARYGTASTANARDGSTPSTYARHNPYATCNARCTPPP 693
           S P +    G AS+ + R      T+ RHNPY   +     PP
Sbjct: 41  SGPGSDMWLGDASSTSPRSLRKTRTFDRHNPYLVSSYATPQPP 83


>At5g24350.1 68418.m02870 expressed protein weak similarity to
            neuroblastoma-amplified protein [Homo sapiens] GI:4337460
          Length = 2376

 Score = 28.3 bits (60), Expect = 5.2
 Identities = 12/41 (29%), Positives = 24/41 (58%)
 Frame = +1

Query: 355  EHLRRQCLVWVRPLHLCQIWDHLHRQCLVWVLPLHLCQVWD 477
            E ++++C+V   PLH C  W  + R+ +   +P ++ Q+ D
Sbjct: 2104 EKVKKECVVSAHPLHSC--WLDIFRKYIALSMPENVLQLID 2142


>At5g03280.1 68418.m00277 ethylene-insensitive 2 (EIN2) identical to
           EIN2 [Arabidopsis thaliana] gi|5231113|gb|AAD41076;
           member of the natural resistance-associated macrophage
           protein (NRAMP) metal transporter family, PMID:11500563;
           metal transport capacity has not been shown,
           PMID:11500563, PMID:1038174
          Length = 1294

 Score = 28.3 bits (60), Expect = 5.2
 Identities = 13/41 (31%), Positives = 19/41 (46%)
 Frame = +2

Query: 290 YGTTTTPNAWYWSAPSTYARYRSTSAANAWYGSAPSTYARY 412
           YG TT  N  Y  +   Y+  R+ S++  W    P+T   Y
Sbjct: 783 YGNTTNNNNAYELSERRYSSLRAPSSSEGWEHQQPATVHGY 823


>At4g14300.1 68417.m02203 heterogeneous nuclear ribonucleoprotein,
           putative / hnRNP, putative 
          Length = 411

 Score = 28.3 bits (60), Expect = 5.2
 Identities = 17/56 (30%), Positives = 26/56 (46%)
 Frame = +2

Query: 341 YARYRSTSAANAWYGSAPSTYARYGTTSTANA*YGSSPSTYARYGTTSTANA*YGS 508
           Y  Y   + +   YG+  +  A YG T+   A YGSS     R    + A++ YG+
Sbjct: 257 YGAYGGYTGSAGGYGAGAT--AGYGATNIPGAGYGSSTGVAPRNSWDTPASSGYGN 310



 Score = 28.3 bits (60), Expect = 5.2
 Identities = 24/83 (28%), Positives = 38/83 (45%), Gaps = 8/83 (9%)
 Frame = +2

Query: 284 ARYGTTTTPNAWYWSAPSTYARYRSTSAANAWYGS----APSTYARYGTTSTANA*YGSS 451
           A YG T  P A Y S+     R    + A++ YG+    + + ++ YG    A      S
Sbjct: 276 AGYGATNIPGAGYGSSTGVAPRNSWDTPASSGYGNPGYGSGAAHSGYGVPGAAPP--TQS 333

Query: 452 PSTYAR----YGTTSTANA*YGS 508
           PS Y+     YG  S +++ YG+
Sbjct: 334 PSGYSNQGYGYGGYSGSDSGYGN 356


>At3g03780.2 68416.m00387
           5-methyltetrahydropteroyltriglutamate--homocysteine
           methyltransferase, putative / vitamin-B12-independent
           methionine synthase, putative / cobalamin-independent
           methionine synthase, putative very strong similarity to
           SP|O50008
           5-methyltetrahydropteroyltriglutamate--homocysteine
           methyltransferase (EC 2.1.1.14) (Vitamin-B12-independent
           methionine synthase isozyme) (Cobalamin-independent
           methionine synthase isozyme) {Arabidopsis thaliana};
           contains Pfam profile PF01717: Methionine synthase,
           vitamin-B12 independent
          Length = 765

 Score = 27.5 bits (58), Expect = 9.0
 Identities = 9/38 (23%), Positives = 18/38 (47%)
 Frame = +1

Query: 469 VWDHLHRQCLVWVPPSTYARYGTTSTANAWYGSAPSTY 582
           +W  +    + ++P +T++ Y       A  G+ PS Y
Sbjct: 45  IWKQMSAAGIKYIPSNTFSHYDQVLDTTAMLGAVPSRY 82


>At3g03780.1 68416.m00386
           5-methyltetrahydropteroyltriglutamate--homocysteine
           methyltransferase, putative / vitamin-B12-independent
           methionine synthase, putative / cobalamin-independent
           methionine synthase, putative very strong similarity to
           SP|O50008
           5-methyltetrahydropteroyltriglutamate--homocysteine
           methyltransferase (EC 2.1.1.14) (Vitamin-B12-independent
           methionine synthase isozyme) (Cobalamin-independent
           methionine synthase isozyme) {Arabidopsis thaliana};
           contains Pfam profile PF01717: Methionine synthase,
           vitamin-B12 independent
          Length = 765

 Score = 27.5 bits (58), Expect = 9.0
 Identities = 9/38 (23%), Positives = 18/38 (47%)
 Frame = +1

Query: 469 VWDHLHRQCLVWVPPSTYARYGTTSTANAWYGSAPSTY 582
           +W  +    + ++P +T++ Y       A  G+ PS Y
Sbjct: 45  IWKQMSAAGIKYIPSNTFSHYDQVLDTTAMLGAVPSRY 82


>At1g20110.1 68414.m02516 zinc finger (FYVE type) family protein
           contains Pfam profile: PF01363 FYVE zinc finger
          Length = 601

 Score = 27.5 bits (58), Expect = 9.0
 Identities = 19/65 (29%), Positives = 26/65 (40%), Gaps = 3/65 (4%)
 Frame = +1

Query: 508 PPSTYARYGTTSTANAWYGSAPSTYARYGTASTANARDGSTP-STYARHNPYA--TCNAR 678
           PP+     G T      Y SAP     YG+A  +N     TP    + H P +  +  + 
Sbjct: 32  PPAPATVAGPTDLTRNTYASAPPFTGGYGSADYSNYSQNYTPYGQNSEHVPPSAPSFTSP 91

Query: 679 CTPPP 693
             PPP
Sbjct: 92  SQPPP 96


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,911,589
Number of Sequences: 28952
Number of extensions: 258093
Number of successful extensions: 677
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 595
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 675
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1496852856
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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