BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00771 (818 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC926.04c |hsp90|swo1|heat shock protein Hsp90|Schizosaccharom... 125 9e-30 SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy... 29 0.79 SPAC3H1.04c |mdm31||mitochondrial inner membrane protein Mdm31|S... 27 2.4 SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 27 4.2 SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25 |Schizosac... 26 5.6 SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces pombe... 26 5.6 SPBC30D10.17c |||glucan synthase regulator |Schizosaccharomyces ... 26 7.4 SPAC19B12.01 ||SPAC4F10.21|TPR repeat protein, TTC27 family|Schi... 25 9.8 SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schi... 25 9.8 >SPAC926.04c |hsp90|swo1|heat shock protein Hsp90|Schizosaccharomyces pombe|chr 1|||Manual Length = 704 Score = 125 bits (301), Expect = 9e-30 Identities = 59/84 (70%), Positives = 70/84 (83%) Frame = +2 Query: 257 TDPSKLDSGKELYIKIIPNKNEGTLTIIDTGIGMTKADLVNNLGTIAKSGTKAFMEALQA 436 +DP LD+ K+L+I+I P+K L+I DTGIGMTK DL+NNLG IAKSGTK FMEA + Sbjct: 52 SDPHALDAEKDLFIRITPDKENKILSIRDTGIGMTKNDLINNLGVIAKSGTKQFMEAAAS 111 Query: 437 GADISMIGQFGVGFYSSYLVADRV 508 GADISMIGQFGVGFYS+YLVAD+V Sbjct: 112 GADISMIGQFGVGFYSAYLVADKV 135 Score = 84.2 bits (199), Expect = 2e-17 Identities = 40/49 (81%), Positives = 45/49 (91%) Frame = +3 Query: 108 AEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYES 254 + ETF F+AEI+QLMSLIINT YSNKEIFLRELISN+SDALDKIRY+S Sbjct: 2 SNTETFKFEAEISQLMSLIINTVYSNKEIFLRELISNASDALDKIRYQS 50 Score = 70.1 bits (164), Expect = 3e-13 Identities = 36/68 (52%), Positives = 44/68 (64%), Gaps = 1/68 (1%) Frame = +1 Query: 511 VHSKHNDDEQYVWESSAGGSFTVRPDSGEP-LGRGTKIVLHVKEDLAEFMXXXXXXXXXX 687 V SKHNDDEQY+WESSAGGSFTV D+ P L RGT+I L +KED +++ Sbjct: 137 VVSKHNDDEQYIWESSAGGSFTVTLDTDGPRLLRGTEIRLFMKEDQLQYLEEKTIKDTVK 196 Query: 688 XXSQFIGY 711 S+FI Y Sbjct: 197 KHSEFISY 204 >SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 541 Score = 29.1 bits (62), Expect = 0.79 Identities = 22/66 (33%), Positives = 35/66 (53%), Gaps = 5/66 (7%) Frame = +3 Query: 180 SNKEIFLRELISNSSDALDKIRYESSRIRQNSI-VAKSCTSRSFPTRTRALLRSSI---- 344 S+ +L +L+++ D L K+R E RIR+ I V S T TRA+++ + Sbjct: 325 SSAMTWLFQLLADHPDVLQKVREEQLRIRKGDIDVPLSLDLMEKMTYTRAVVKECLRLRP 384 Query: 345 PVLV*P 362 PVL+ P Sbjct: 385 PVLMVP 390 >SPAC3H1.04c |mdm31||mitochondrial inner membrane protein Mdm31|Schizosaccharomyces pombe|chr 1|||Manual Length = 601 Score = 27.5 bits (58), Expect = 2.4 Identities = 20/53 (37%), Positives = 30/53 (56%) Frame = -3 Query: 417 KALVPDFAMVPKLFTKSALVIPIPVSMIVRVPSFLLGMILMYSSLPLSSFDGS 259 KA++P F + +AL+ PI ++ I +F+ IL + S PLS FDGS Sbjct: 486 KAVIPIFTNQVS-YINNALIRPI-IAYINSTRTFI--PILCHVSKPLSDFDGS 534 >SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1044 Score = 26.6 bits (56), Expect = 4.2 Identities = 14/64 (21%), Positives = 32/64 (50%) Frame = +3 Query: 81 MPEEMETQPAEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYESSR 260 + E++ + A+ E+F A+L +N NKE + +L + S+ +++ S+ Sbjct: 762 LTEKLNKKNADTESFKNTIREAELSKKALNDNLGNKENIISDLKNKLSEESTRLQELQSQ 821 Query: 261 IRQN 272 + Q+ Sbjct: 822 LNQD 825 >SPBC146.05c |cwf25||complexed with Cdc5 protein Cwf25 |Schizosaccharomyces pombe|chr 2|||Manual Length = 376 Score = 26.2 bits (55), Expect = 5.6 Identities = 15/50 (30%), Positives = 25/50 (50%), Gaps = 1/50 (2%) Frame = +1 Query: 238 KSGMNLHGSVKTR*WQRAVHQDHSQ-QERGHSYDHRYRYWYDQGRFGEQF 384 +S H S K +R+ D + +ER H+ +R+R YD G F + + Sbjct: 187 RSDNEQHSSDKREHSRRSYRNDRNNWRERTHNDRYRHRDKYDSGYFKKHY 236 >SPACUNK4.12c |mug138||metallopeptidase|Schizosaccharomyces pombe|chr 1|||Manual Length = 969 Score = 26.2 bits (55), Expect = 5.6 Identities = 14/36 (38%), Positives = 19/36 (52%) Frame = -2 Query: 394 DGSQIVHQIGLGHTNTGIDDRKSALVLVGNDLDVQL 287 DG V I G +DDR+ L+ + NDL+V L Sbjct: 2 DGKPQVEVIVNGQVVPNLDDREYRLIKLENDLEVLL 37 >SPBC30D10.17c |||glucan synthase regulator |Schizosaccharomyces pombe|chr 2|||Manual Length = 504 Score = 25.8 bits (54), Expect = 7.4 Identities = 18/76 (23%), Positives = 33/76 (43%), Gaps = 1/76 (1%) Frame = +2 Query: 494 VADRVLFTLNTMTTSNTCGN-LLQEARSQSAQTAVSPLVEVQRSSFTSKRTWQNSWKNTK 670 +A+ V ++TT N + + +R + +PL V S+++ Q N Sbjct: 9 MANSVTSFFQSLTTPNRHADPSFRPSRREKQSRLPTPLQSVAASAYSGVNASQTGLLNDS 68 Query: 671 SKRS*RNIPSSLATNQ 718 S N+P+S T+Q Sbjct: 69 RANSVTNLPNSSNTSQ 84 >SPAC19B12.01 ||SPAC4F10.21|TPR repeat protein, TTC27 family|Schizosaccharomyces pombe|chr 1|||Manual Length = 817 Score = 25.4 bits (53), Expect = 9.8 Identities = 8/29 (27%), Positives = 16/29 (55%) Frame = +1 Query: 298 QDHSQQERGHSYDHRYRYWYDQGRFGEQF 384 +DH++++ H+ +Y YD R E + Sbjct: 603 KDHTKEQAWHAMQQGIKYMYDNWRIWENY 631 >SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 3071 Score = 25.4 bits (53), Expect = 9.8 Identities = 12/43 (27%), Positives = 22/43 (51%) Frame = +2 Query: 260 DPSKLDSGKELYIKIIPNKNEGTLTIIDTGIGMTKADLVNNLG 388 DPSK ++Y K+ + + L D+GI ++ L+ +G Sbjct: 2472 DPSKAVRKPKIYSKVSTEERDFNLEQFDSGIDLSVKFLLEGIG 2514 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,237,685 Number of Sequences: 5004 Number of extensions: 71020 Number of successful extensions: 215 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 205 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 215 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 400438000 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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