BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00758 (793 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual 37 0.004 SPBC30B4.02c |||R3H and G-patch domain, unknown biological role|... 33 0.062 SPAC23C4.19 |spt5||transcription elongation factor Spt5|Schizosa... 32 0.082 SPAC15A10.15 |sgo2||shugoshin Sgo2|Schizosaccharomyces pombe|chr... 31 0.14 SPBP8B7.20c |||RNA methyltransferase Nop2 |Schizosaccharomyces p... 31 0.19 SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |S... 29 0.58 SPBC839.12 |rpc31||DNA-directed RNA polymerase III complex subun... 28 1.3 SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 28 1.3 SPCC645.12c |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 28 1.3 SPAC167.03c |snu66||U4/U6 x U5 tri-snRNP complex subunit Snu66 |... 28 1.8 SPAC1687.12c |coq4||ubiquinone biosynthesis protein Coq4|Schizos... 28 1.8 SPAC23H3.06 |apl6||AP-3 adaptor complex subunit Apl6 |Schizosacc... 27 2.3 SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 27 2.3 SPCC4G3.09c |gyp3||GTPase activating protein Gyp3|Schizosaccharo... 27 3.1 SPCC663.01c |ekc1|SPCC777.16c|protein phosphatase regulatory sub... 27 4.1 SPCC550.15c |||ribosome biogenesis protein |Schizosaccharomyces ... 27 4.1 SPAC23C11.03 |||U3 snoRNP-associated protein Mpp1 |Schizosacchar... 26 7.1 SPAC2G11.14 |taf111|taf1, taf1, taf130|transcription factor TFII... 26 7.1 SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 26 7.1 SPBC14C8.17c |||SAGA complex subunit Spt8 |Schizosaccharomyces p... 26 7.1 SPAPB1A10.07c |||sphingolipid biosynthesis protein|Schizosacchar... 26 7.1 SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|S... 25 9.4 SPCC1919.01 |ppk34|SPCC830.12|serine/threonine protein kinase Pp... 25 9.4 SPAC959.04c |||mannosyltransferase |Schizosaccharomyces pombe|ch... 25 9.4 SPBC21D10.12 |hob1||BAR adaptor protein Hob1|Schizosaccharomyces... 25 9.4 >SPCC737.08 |||midasin |Schizosaccharomyces pombe|chr 3|||Manual Length = 4717 Score = 36.7 bits (81), Expect = 0.004 Identities = 23/82 (28%), Positives = 46/82 (56%), Gaps = 8/82 (9%) Frame = +3 Query: 498 QQQTFNDFKPMEDTIYADEDQDLSTLETADKEE----DKDKSNYDSLEKLHDITVLEE-- 659 Q Q F+D + M + DE++DL L A+K++ D+D+S + + ++ + E+ Sbjct: 4303 QSQAFDDSEFMH--VKEDEEEDLQALGNAEKDQIKSIDRDESANQNPDSMNSTNIAEDEA 4360 Query: 660 DNIGDD--EDMLDVADDPESGD 719 D +GD +D D++D ++G+ Sbjct: 4361 DEVGDKQLQDGQDISDIKQTGE 4382 Score = 29.9 bits (64), Expect = 0.44 Identities = 20/70 (28%), Positives = 36/70 (51%), Gaps = 5/70 (7%) Frame = +3 Query: 516 DFKPMEDTIYADEDQDLSTLETADKEEDKDKSNYDSLEKLHDI-TVLEEDNIGDD----E 680 D K E +D DL ++ +E+K++++ + E + D LEE+N D+ + Sbjct: 4089 DLKLDEKEGDVSKDSDLEDMDMEAADENKEEADAEKDEPMQDFEDPLEENNTLDEDIQQD 4148 Query: 681 DMLDVADDPE 710 D D+A+D E Sbjct: 4149 DFSDLAEDDE 4158 Score = 27.9 bits (59), Expect = 1.8 Identities = 21/80 (26%), Positives = 35/80 (43%) Frame = +3 Query: 465 VGKCEIT*PKIQQQTFNDFKPMEDTIYADEDQDLSTLETADKEEDKDKSNYDSLEKLHDI 644 VG +IT + + EDT DL E + E D + DS+ ++ Sbjct: 3918 VGAEDITNTLNEDDDLEELANEEDTA---NQSDLDESEARELESDMNGVTKDSVVSENEN 3974 Query: 645 TVLEEDNIGDDEDMLDVADD 704 + EE+N DE++ D+ +D Sbjct: 3975 SDSEEENQDLDEEVNDIPED 3994 >SPBC30B4.02c |||R3H and G-patch domain, unknown biological role|Schizosaccharomyces pombe|chr 2|||Manual Length = 695 Score = 32.7 bits (71), Expect = 0.062 Identities = 17/57 (29%), Positives = 27/57 (47%) Frame = +3 Query: 513 NDFKPMEDTIYADEDQDLSTLETADKEEDKDKSNYDSLEKLHDITVLEEDNIGDDED 683 N+ P A E + D ++ DK ++ K D++VLEED+ +DED Sbjct: 217 NNAAPPPPLAQAQEQLSTENEDEFDIDDTTDKMTTFTMNKFADLSVLEEDDDDEDED 273 Score = 28.7 bits (61), Expect = 1.0 Identities = 33/109 (30%), Positives = 46/109 (42%), Gaps = 12/109 (11%) Frame = +3 Query: 393 NREGDPHSPLTERNR*SYCKHDCDVGKCEIT*PKIQQQTFNDFKPM----EDTIYADEDQ 560 N P PL + +++ D + T K+ T N F + ED DED+ Sbjct: 216 NNNAAPPPPLAQAQEQLSTENE-DEFDIDDTTDKMTTFTMNKFADLSVLEEDDDDEDEDE 274 Query: 561 DLSTLETADKEEDKD---KSNYDSLE-----KLHDITVLEEDNIGDDED 683 +L E ++EE+K+ SNYD E L D LE D DD D Sbjct: 275 ELEG-EKEEEEEEKEKPEISNYDETEGKVNFSLEDSESLEFDEGSDDYD 322 Score = 26.2 bits (55), Expect = 5.4 Identities = 16/36 (44%), Positives = 22/36 (61%), Gaps = 3/36 (8%) Frame = +3 Query: 612 NYDSLEKLHDITVLEEDNIGDDEDML---DVADDPE 710 N DSL + D +LEED D+E++L D +DD E Sbjct: 333 NTDSLAEDED-DILEEDEDEDEEELLAIFDASDDSE 367 >SPAC23C4.19 |spt5||transcription elongation factor Spt5|Schizosaccharomyces pombe|chr 1|||Manual Length = 990 Score = 32.3 bits (70), Expect = 0.082 Identities = 23/67 (34%), Positives = 35/67 (52%), Gaps = 3/67 (4%) Frame = +3 Query: 525 PMEDTIYADED---QDLSTLETADKEEDKDKSNYDSLEKLHDITVLEEDNIGDDEDMLDV 695 P + TI ED ++ + DK+ED+D N + E+ D EE++ DDE+ D Sbjct: 69 PNDTTINKGEDGNENEVDNVNNNDKKEDED--NVEENEEEADANEEEEEDEEDDEE--DE 124 Query: 696 ADDPESG 716 D+ ESG Sbjct: 125 EDEDESG 131 >SPAC15A10.15 |sgo2||shugoshin Sgo2|Schizosaccharomyces pombe|chr 1|||Manual Length = 647 Score = 31.5 bits (68), Expect = 0.14 Identities = 20/64 (31%), Positives = 35/64 (54%), Gaps = 4/64 (6%) Frame = +3 Query: 498 QQQTFNDFKPMEDTIYADEDQDLSTLE----TADKEEDKDKSNYDSLEKLHDITVLEEDN 665 QQ+ + FKP+E + D D D L+ D EE +++ +D L +++ +E++N Sbjct: 99 QQEIQDTFKPVEANLAYDVDTDSEDLDEESVVKDTEEIIEQAQHD--VSLRNLSGIEDEN 156 Query: 666 IGDD 677 I DD Sbjct: 157 IIDD 160 >SPBP8B7.20c |||RNA methyltransferase Nop2 |Schizosaccharomyces pombe|chr 2|||Manual Length = 608 Score = 31.1 bits (67), Expect = 0.19 Identities = 21/64 (32%), Positives = 36/64 (56%), Gaps = 1/64 (1%) Frame = +3 Query: 498 QQQTFNDFKPMEDT-IYADEDQDLSTLETADKEEDKDKSNYDSLEKLHDITVLEEDNIGD 674 + +T +D + E+ +ADE D E +EED S +DS E+ H++ + D+ GD Sbjct: 74 EAETLSDLEHDEEPQTFADEFIDDEAKECEGEEED---SVFDSDEE-HEVKPMFSDDSGD 129 Query: 675 DEDM 686 +ED+ Sbjct: 130 EEDL 133 >SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |Schizosaccharomyces pombe|chr 2|||Manual Length = 1944 Score = 29.5 bits (63), Expect = 0.58 Identities = 13/63 (20%), Positives = 32/63 (50%) Frame = +3 Query: 489 PKIQQQTFNDFKPMEDTIYADEDQDLSTLETADKEEDKDKSNYDSLEKLHDITVLEEDNI 668 PK+ + F D + + E+Q + +E + ++D++ +N K +D + + D++ Sbjct: 1365 PKVIRVGFGDSISVHAKEFTLEEQMIKQMELTNLKKDQEANNSSDTRKKYDSIIKKRDSL 1424 Query: 669 GDD 677 +D Sbjct: 1425 RED 1427 >SPBC839.12 |rpc31||DNA-directed RNA polymerase III complex subunit Rpc31|Schizosaccharomyces pombe|chr 2|||Manual Length = 210 Score = 28.3 bits (60), Expect = 1.3 Identities = 16/59 (27%), Positives = 31/59 (52%), Gaps = 3/59 (5%) Frame = +3 Query: 570 TLETADKEEDKDKSNYDSLEKLHDITVLEEDNIGDDEDMLDVAD---DPESGDWYPDFD 737 T++ D +++ ++ + ++E+ D + EED D+D D + D GD Y D+D Sbjct: 144 TIKAQDLKDESSEAAHPNIEEEPDEGLEEEDEDFGDDDDNDYGENYFDNGEGDDYDDYD 202 >SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1647 Score = 28.3 bits (60), Expect = 1.3 Identities = 16/70 (22%), Positives = 33/70 (47%), Gaps = 1/70 (1%) Frame = +3 Query: 513 NDFKPMEDTIYAD-EDQDLSTLETADKEEDKDKSNYDSLEKLHDITVLEEDNIGDDEDML 689 + + +D +Y D D + + ++D+ S +S E+ D+ EE+ DD++ Sbjct: 206 SQYNDQDDHVYDDTNDGTDDDINNNEYKDDESSSGSESYERDEDVDEEEEE---DDDENN 262 Query: 690 DVADDPESGD 719 D DD + + Sbjct: 263 DEGDDEDENE 272 >SPCC645.12c |||sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 198 Score = 28.3 bits (60), Expect = 1.3 Identities = 16/67 (23%), Positives = 36/67 (53%), Gaps = 4/67 (5%) Frame = +3 Query: 501 QQTFNDFKPMEDTIYADEDQDLSTLET----ADKEEDKDKSNYDSLEKLHDITVLEEDNI 668 ++ +NDF+ I + + TL+ A+K+E+ + ++ D +++DI EE + Sbjct: 91 EEEWNDFQNEIGIIEENAVEQEITLQQQQLLAEKDEENEIADNDLEPEVYDILYEEESKL 150 Query: 669 GDDEDML 689 G+ D++ Sbjct: 151 GESRDLI 157 >SPAC167.03c |snu66||U4/U6 x U5 tri-snRNP complex subunit Snu66 |Schizosaccharomyces pombe|chr 1|||Manual Length = 649 Score = 27.9 bits (59), Expect = 1.8 Identities = 14/45 (31%), Positives = 25/45 (55%) Frame = +3 Query: 420 LTERNR*SYCKHDCDVGKCEIT*PKIQQQTFNDFKPMEDTIYADE 554 +T++ R Y K + + +I +I+ + F+D+KP D Y DE Sbjct: 559 MTQKEREQYAKKENERWDKKIA--EIELEQFHDYKPQVDIKYVDE 601 >SPAC1687.12c |coq4||ubiquinone biosynthesis protein Coq4|Schizosaccharomyces pombe|chr 1|||Manual Length = 272 Score = 27.9 bits (59), Expect = 1.8 Identities = 16/44 (36%), Positives = 25/44 (56%) Frame = +1 Query: 475 VKLLNQKYNNKPSTTSNLWRILYTLTKTKI*AP*KLPIRKKTRI 606 V L++K+ KPS SN R+L+T + K+ P +P+ RI Sbjct: 17 VMSLSKKFL-KPSVASNQLRLLFTAAERKVNYPGHVPLSPLQRI 59 >SPAC23H3.06 |apl6||AP-3 adaptor complex subunit Apl6 |Schizosaccharomyces pombe|chr 1|||Manual Length = 745 Score = 27.5 bits (58), Expect = 2.3 Identities = 17/49 (34%), Positives = 27/49 (55%), Gaps = 1/49 (2%) Frame = +3 Query: 489 PK-IQQQTFNDFKPMEDTIYADEDQDLSTLETADKEEDKDKSNYDSLEK 632 PK + QQT +F E + ++ + + ST E+ D+E D D S + EK Sbjct: 662 PKFVGQQTLEEFYASETSESSEGEYETSTSESEDEETD-DTSQEEDNEK 709 >SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Schizosaccharomyces pombe|chr 1|||Manual Length = 1995 Score = 27.5 bits (58), Expect = 2.3 Identities = 18/60 (30%), Positives = 28/60 (46%), Gaps = 2/60 (3%) Frame = +3 Query: 528 MEDTIYADEDQDLSTLETADKEEDKDKSNYDSLEKLHDITVLEEDNIGDDE--DMLDVAD 701 +E T+ DE+ S E D +E K +EK+ T +E I + + D LD+ D Sbjct: 161 LETTLKGDENYSSSQKEIYDNDETIPKPPSPEVEKIETSTSVENAYITNSQYNDTLDMED 220 >SPCC4G3.09c |gyp3||GTPase activating protein Gyp3|Schizosaccharomyces pombe|chr 3|||Manual Length = 635 Score = 27.1 bits (57), Expect = 3.1 Identities = 18/57 (31%), Positives = 27/57 (47%), Gaps = 2/57 (3%) Frame = +3 Query: 528 MEDTIYADEDQDLS--TLETADKEEDKDKSNYDSLEKLHDITVLEEDNIGDDEDMLD 692 M D + +E+ LS ++ E+DK S+Y+ L D+ L D DD D D Sbjct: 11 MNDANFKEENAHLSDASIHFGSSEQDKTPSDYE----LDDVLDLYNDTTEDDADDAD 63 >SPCC663.01c |ekc1|SPCC777.16c|protein phosphatase regulatory subunit Ekc1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 838 Score = 26.6 bits (56), Expect = 4.1 Identities = 18/70 (25%), Positives = 33/70 (47%), Gaps = 4/70 (5%) Frame = +3 Query: 534 DTIYADEDQDLSTLETADKEEDKDKSNYDSLEKLHD-ITVLEEDNIG---DDEDMLDVAD 701 D + +DED D ST + ++ K+ + + E +H + +DN+ D M + Sbjct: 407 DLVMSDEDDDDSTFSDKNSKDFKETEDMNGAEDMHGRAPQITKDNLNLTTTDSPMSEA-- 464 Query: 702 DPESGDWYPD 731 +P S + Y D Sbjct: 465 EPVSEEEYKD 474 >SPCC550.15c |||ribosome biogenesis protein |Schizosaccharomyces pombe|chr 3|||Manual Length = 463 Score = 26.6 bits (56), Expect = 4.1 Identities = 12/41 (29%), Positives = 20/41 (48%) Frame = +3 Query: 507 TFNDFKPMEDTIYADEDQDLSTLETADKEEDKDKSNYDSLE 629 ++ D+ +D +ED + D +D D S+ DSLE Sbjct: 306 SYPDYAVKQDETVVEEDGSSGEGDWEDVSDDSDNSSLDSLE 346 Score = 26.2 bits (55), Expect = 5.4 Identities = 12/35 (34%), Positives = 19/35 (54%) Frame = +3 Query: 609 SNYDSLEKLHDITVLEEDNIGDDEDMLDVADDPES 713 ++Y D TV+EED + D DV+DD ++ Sbjct: 305 TSYPDYAVKQDETVVEEDGSSGEGDWEDVSDDSDN 339 >SPAC23C11.03 |||U3 snoRNP-associated protein Mpp1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 598 Score = 25.8 bits (54), Expect = 7.1 Identities = 17/74 (22%), Positives = 33/74 (44%), Gaps = 2/74 (2%) Frame = +3 Query: 495 IQQQTFNDFKPMEDTIYADEDQDLSTLETADKEEDK--DKSNYDSLEKLHDITVLEEDNI 668 + + + D E I +E Q + + A D D N++ + +EE + Sbjct: 128 VDESSAEDVSGDEAEIMNEEGQQIEQKKDAFGLNDGFFDIDNFNKQTLALEEEAVEEGVL 187 Query: 669 GDDEDMLDVADDPE 710 GD++D +D+ DP+ Sbjct: 188 GDEDDGIDLLMDPD 201 >SPAC2G11.14 |taf111|taf1, taf1, taf130|transcription factor TFIID complex subunit Taf111|Schizosaccharomyces pombe|chr 1|||Manual Length = 979 Score = 25.8 bits (54), Expect = 7.1 Identities = 12/40 (30%), Positives = 21/40 (52%) Frame = +3 Query: 585 DKEEDKDKSNYDSLEKLHDITVLEEDNIGDDEDMLDVADD 704 + +D NY+ + + I +NI + +DM DV+DD Sbjct: 61 ENSNPQDGPNYEDFDFMGSIHKEFGNNINEMDDMEDVSDD 100 >SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces pombe|chr 1|||Manual Length = 143 Score = 25.8 bits (54), Expect = 7.1 Identities = 19/52 (36%), Positives = 24/52 (46%), Gaps = 3/52 (5%) Frame = +3 Query: 597 DKDKSNYDSLEKLHDITVLEEDNIGDDEDMLDV--ADDPESGDW-YPDFDKR 743 DKD S Y K D + + D+E L V AD SG + Y DF +R Sbjct: 88 DKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEADPTNSGSFDYYDFVQR 139 >SPBC14C8.17c |||SAGA complex subunit Spt8 |Schizosaccharomyces pombe|chr 2|||Manual Length = 526 Score = 25.8 bits (54), Expect = 7.1 Identities = 11/34 (32%), Positives = 21/34 (61%) Frame = +3 Query: 534 DTIYADEDQDLSTLETADKEEDKDKSNYDSLEKL 635 DT+ + + D T +T++K+E ++ + LEKL Sbjct: 64 DTMEDENENDEDTEQTSEKKETEETPKENPLEKL 97 >SPAPB1A10.07c |||sphingolipid biosynthesis protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 441 Score = 25.8 bits (54), Expect = 7.1 Identities = 14/29 (48%), Positives = 19/29 (65%), Gaps = 2/29 (6%) Frame = +3 Query: 540 IYADE-DQDLST-LETADKEEDKDKSNYD 620 +YAD D +ST LE EEDK +S+Y+ Sbjct: 337 LYADSHDMGVSTPLEDGSSEEDKHQSDYN 365 >SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|Schizosaccharomyces pombe|chr 2|||Manual Length = 2337 Score = 25.4 bits (53), Expect = 9.4 Identities = 14/42 (33%), Positives = 21/42 (50%) Frame = +3 Query: 603 DKSNYDSLEKLHDITVLEEDNIGDDEDMLDVADDPESGDWYP 728 D + + LEK + V E+ NI D +L P S ++YP Sbjct: 763 DPNRHRVLEKGTEKVVPEQKNIPPDISLLMSGIGPSSDEYYP 804 >SPCC1919.01 |ppk34|SPCC830.12|serine/threonine protein kinase Ppk34|Schizosaccharomyces pombe|chr 3|||Manual Length = 354 Score = 25.4 bits (53), Expect = 9.4 Identities = 12/47 (25%), Positives = 25/47 (53%) Frame = +3 Query: 528 MEDTIYADEDQDLSTLETADKEEDKDKSNYDSLEKLHDITVLEEDNI 668 +E + + D D ST+E + ++K Y ++L + VL+ +N+ Sbjct: 87 LEKELGSFNDMDSSTIEDQIRNQEKKDPYYYIRKELDVLKVLDHENV 133 >SPAC959.04c |||mannosyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 298 Score = 25.4 bits (53), Expect = 9.4 Identities = 8/15 (53%), Positives = 9/15 (60%) Frame = -1 Query: 175 HHCVNTAPANGRCHC 131 H+C T P GRC C Sbjct: 283 HYCPETIPYTGRCAC 297 >SPBC21D10.12 |hob1||BAR adaptor protein Hob1|Schizosaccharomyces pombe|chr 2|||Manual Length = 466 Score = 25.4 bits (53), Expect = 9.4 Identities = 13/43 (30%), Positives = 21/43 (48%) Frame = +3 Query: 489 PKIQQQTFNDFKPMEDTIYADEDQDLSTLETADKEEDKDKSNY 617 P+ + FN + +D IY D + +LET K+ +D Y Sbjct: 14 PQTLRSKFNVGEITKDPIYEDAGRRFKSLETEAKKLAEDAKKY 56 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,974,345 Number of Sequences: 5004 Number of extensions: 60409 Number of successful extensions: 245 Number of sequences better than 10.0: 25 Number of HSP's better than 10.0 without gapping: 215 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 240 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 385381248 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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