Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00755
(777 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.03c |||2'-O-ribose methyltransferase|Schizosaccharomyce... 97 2e-21
SPAC1687.11 |spb1||rRNA methyltransferase Spb1 |Schizosaccharomy... 58 1e-09
SPBC2G2.15c |||mitochondrial 2' O-ribose methyltransferase Mrm2 ... 38 0.002
SPBC119.17 ||SPBC577.01|metallopeptidase|Schizosaccharomyces pom... 27 2.3
SPBC17A3.03c |||phosphoprotein phosphatase |Schizosaccharomyces ... 27 4.0
SPCC613.07 |||zf-HIT|Schizosaccharomyces pombe|chr 3|||Manual 27 4.0
SPAC19G12.12 |dlp1||decaprenyl diphosphate synthase subunit 2 Dl... 26 5.2
SPBC215.11c |||aldo/keto reductase, unknown biological role|Schi... 26 6.9
SPAC1006.05c |och1||alpha-1,6-mannosyltransferase Och1 |Schizosa... 26 6.9
SPAC18G6.05c |||translation elongation regulator Gcn1 |Schizosac... 25 9.2
>SPAC4F10.03c |||2'-O-ribose methyltransferase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 285
Score = 97.5 bits (232), Expect = 2e-21
Identities = 50/86 (58%), Positives = 59/86 (68%)
Frame = +2
Query: 260 IVAVDLQAMAALPGVKQIQGDITKQETANAIIEEFQGLKADLVVCDGAPDVTGLHDIDEY 439
IVAVDLQ MA + GV +Q DIT T + I+ F ADLVV DGAPDVTGLHD+DEY
Sbjct: 79 IVAVDLQPMAPIDGVCTLQLDITHPNTLSIILSHFGNEPADLVVSDGAPDVTGLHDLDEY 138
Query: 440 VQSQLLLAALNITTHVLKNEGTFWPK 517
+Q+Q+LLAA N+ VLK G F K
Sbjct: 139 IQAQILLAAFNLAVCVLKPGGKFVAK 164
Score = 90.2 bits (214), Expect = 3e-19
Identities = 38/50 (76%), Positives = 45/50 (90%)
Frame = +1
Query: 40 MGKTSKDKRDIYYRLAKEEGWRARSAFKLLQINEEYNIFNGVLRAVDLCA 189
MG++SKDKRD YYRLAKE+GWRARSAFKLLQ+NE++N+F G R VDLCA
Sbjct: 1 MGRSSKDKRDAYYRLAKEQGWRARSAFKLLQLNEQFNLFEGAKRVVDLCA 50
Score = 86.2 bits (204), Expect = 5e-18
Identities = 37/60 (61%), Positives = 50/60 (83%)
Frame = +1
Query: 508 LAKIFRGKDVSLLYSQLKQFFKLVTVSKPRSSRNSSIEAFVICEKYNPPEDYVPNMVNPL 687
+AKIFRG+DVSLLYSQL+ F+ V+ +KPRSSR SSIE+FV+CE +NPP ++ P++ PL
Sbjct: 162 VAKIFRGRDVSLLYSQLRLMFRKVSCAKPRSSRASSIESFVVCEDFNPPSNFQPDLTKPL 221
Score = 27.1 bits (57), Expect = 3.0
Identities = 10/15 (66%), Positives = 13/15 (86%)
Frame = +3
Query: 192 PGSWSQVLTKNLRQN 236
PGSWSQVL++ L +N
Sbjct: 52 PGSWSQVLSRELLKN 66
>SPAC1687.11 |spb1||rRNA methyltransferase Spb1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 802
Score = 58.4 bits (135), Expect = 1e-09
Identities = 24/52 (46%), Positives = 39/52 (75%)
Frame = +1
Query: 34 KNMGKTSKDKRDIYYRLAKEEGWRARSAFKLLQINEEYNIFNGVLRAVDLCA 189
K+ KT+K + D +Y+LAKE+G+R+R+AFKL+Q+N++Y+ +DLCA
Sbjct: 3 KSQKKTAKGRLDKWYKLAKEQGYRSRAAFKLVQLNQKYSFLEKAKVIIDLCA 54
Score = 52.0 bits (119), Expect = 9e-08
Identities = 24/59 (40%), Positives = 32/59 (54%)
Frame = +1
Query: 508 LAKIFRGKDVSLLYSQLKQFFKLVTVSKPRSSRNSSIEAFVICEKYNPPEDYVPNMVNP 684
+ K+FR +D + L KQ F V +KP SSRN S E FV+C Y P+ P +P
Sbjct: 156 VTKVFRSRDYNNLLWVFKQLFNKVEATKPPSSRNVSAEIFVVCRGYKAPKKLDPRFTDP 214
Score = 50.8 bits (116), Expect = 2e-07
Identities = 33/97 (34%), Positives = 49/97 (50%)
Frame = +2
Query: 227 STKCCKY*GFKIVAVDLQAMAALPGVKQIQGDITKQETANAIIEEFQGLKADLVVCDGAP 406
++K CK G IV VDL + +P DIT + + + + KAD+V+ DGAP
Sbjct: 63 ASKTCKP-GSLIVGVDLAPIKPIPNCHTFVEDITSDKCRSQLRGYLKTWKADVVLHDGAP 121
Query: 407 DVTGLHDIDEYVQSQLLLAALNITTHVLKNEGTFWPK 517
+V D Y Q+QL+L ++ + L GTF K
Sbjct: 122 NVGSAWLQDAYGQAQLVLMSMKLACEFLVAGGTFVTK 158
>SPBC2G2.15c |||mitochondrial 2' O-ribose methyltransferase Mrm2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 218
Score = 37.5 bits (83), Expect = 0.002
Identities = 27/100 (27%), Positives = 44/100 (44%), Gaps = 12/100 (12%)
Frame = +2
Query: 257 KIVAVDLQAMAALPGVKQIQGDITKQETANAIIEEFQGLK------------ADLVVCDG 400
K++ +D+Q +A GV I GDI T + E + L D V+ D
Sbjct: 75 KVIGIDIQHIAPPEGVLPIYGDIRDPNTLTKLFEALRLLHEPNTNDSIDCRVVDAVISDM 134
Query: 401 APDVTGLHDIDEYVQSQLLLAALNITTHVLKNEGTFWPKY 520
TG+ D + +L +AL++ LK+ G+F K+
Sbjct: 135 LHKATGIRIRDHALSMELCASALHVALTFLKSNGSFICKF 174
Score = 35.5 bits (78), Expect = 0.009
Identities = 16/48 (33%), Positives = 27/48 (56%)
Frame = +1
Query: 508 LAKIFRGKDVSLLYSQLKQFFKLVTVSKPRSSRNSSIEAFVICEKYNP 651
+ K + G + + L + LK F+ V V KP++S S EA+ +C + P
Sbjct: 171 ICKFYMGDEDADLQNLLKSHFRFVQVMKPKASLKESREAYFVCLERKP 218
Score = 35.1 bits (77), Expect = 0.011
Identities = 12/36 (33%), Positives = 25/36 (69%)
Frame = +1
Query: 52 SKDKRDIYYRLAKEEGWRARSAFKLLQINEEYNIFN 159
+K +D Y + +K + +R+R+A+KL+++N +Y N
Sbjct: 12 AKRSKDFYRKKSKIDNFRSRAAYKLIELNSKYRFIN 47
>SPBC119.17 ||SPBC577.01|metallopeptidase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 992
Score = 27.5 bits (58), Expect = 2.3
Identities = 12/39 (30%), Positives = 17/39 (43%)
Frame = -2
Query: 596 LGLDTVTSLKNCFNCEYSRETSFPLNILAKMYLHS*AHG 480
L T + K+C Y+ PL IL+ + H HG
Sbjct: 812 LPFQTYFAAKSCLGVPYTHPDGAPLQILSSLLTHKYLHG 850
>SPBC17A3.03c |||phosphoprotein phosphatase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 263
Score = 26.6 bits (56), Expect = 4.0
Identities = 18/45 (40%), Positives = 23/45 (51%)
Frame = -2
Query: 671 FGT*SSGGLYFSQITKASMLEFLELLGLDTVTSLKNCFNCEYSRE 537
FG G +Y S +AS FLE L + T+ SL+ EYS E
Sbjct: 62 FGVVYPGIIYRSACPRASNFNFLESLHIRTIISLR---QEEYSEE 103
>SPCC613.07 |||zf-HIT|Schizosaccharomyces pombe|chr 3|||Manual
Length = 345
Score = 26.6 bits (56), Expect = 4.0
Identities = 15/39 (38%), Positives = 21/39 (53%)
Frame = +1
Query: 634 CEKYNPPEDYVPNMVNPLLDHKYFDLNSDFNSFTGINRL 750
C P +VP + L++H LNSDFN +G+ RL
Sbjct: 43 CSGERDPATFVPK--SKLVNH----LNSDFNFLSGVERL 75
>SPAC19G12.12 |dlp1||decaprenyl diphosphate synthase subunit 2 Dlp1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 294
Score = 26.2 bits (55), Expect = 5.2
Identities = 9/14 (64%), Positives = 12/14 (85%)
Frame = +3
Query: 180 SLRRPGSWSQVLTK 221
SL++PGSWS +L K
Sbjct: 20 SLKKPGSWSSILLK 33
>SPBC215.11c |||aldo/keto reductase, unknown biological
role|Schizosaccharomyces pombe|chr 2|||Manual
Length = 306
Score = 25.8 bits (54), Expect = 6.9
Identities = 11/30 (36%), Positives = 18/30 (60%)
Frame = +3
Query: 138 RGIQHI*WCTACSGSLRRPGSWSQVLTKNL 227
+GI I W SG+L +PG+ ++K+L
Sbjct: 207 KGIAFIPWYPLASGALAKPGTILDAVSKDL 236
>SPAC1006.05c |och1||alpha-1,6-mannosyltransferase Och1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 396
Score = 25.8 bits (54), Expect = 6.9
Identities = 14/43 (32%), Positives = 22/43 (51%)
Frame = -1
Query: 345 FAVSCLVISP*ICFTPGNAAIA*RSTATILNPQYLQHFVEGSW 217
+ V ++I P F+PG + +S + Y+QHF GSW
Sbjct: 353 YLVGDVLILPITAFSPGVGHMGSKSPNDPM--AYVQHFFAGSW 393
>SPAC18G6.05c |||translation elongation regulator Gcn1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2670
Score = 25.4 bits (53), Expect = 9.2
Identities = 16/53 (30%), Positives = 26/53 (49%)
Frame = +2
Query: 311 IQGDITKQETANAIIEEFQGLKADLVVCDGAPDVTGLHDIDEYVQSQLLLAAL 469
+ GD+ +ETA + Q + D V D P + L ++ QS+ L+AL
Sbjct: 1996 MDGDLEVRETAAEAFDSLQSILGDRAVDDVLPQLLKL--LESENQSEQALSAL 2046
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,322,622
Number of Sequences: 5004
Number of extensions: 71365
Number of successful extensions: 226
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 209
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 225
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 375345278
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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