Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00754
(832 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g06900.1 68414.m00733 peptidase M16 family protein / insulina... 109 2e-24
At2g41790.1 68415.m05165 peptidase M16 family protein / insulina... 96 2e-20
At3g57470.1 68416.m06398 peptidase M16 family protein / insulina... 67 1e-11
At3g02090.2 68416.m00175 mitochondrial processing peptidase beta... 48 8e-06
At3g02090.1 68416.m00174 mitochondrial processing peptidase beta... 48 8e-06
At5g42390.1 68418.m05161 metalloendopeptidase identical to chlor... 38 0.006
At1g49630.3 68414.m05566 peptidase M16 family protein / insulina... 33 0.18
At1g49630.2 68414.m05565 peptidase M16 family protein / insulina... 33 0.18
At1g49630.1 68414.m05564 peptidase M16 family protein / insulina... 33 0.18
At3g19170.1 68416.m02434 peptidase M16 family protein / insulina... 33 0.23
At4g35240.1 68417.m05009 expressed protein contains Pfam domains... 30 2.2
At5g56730.1 68418.m07080 peptidase M16 family protein / insulina... 29 3.8
At1g53300.1 68414.m06041 thioredoxin family protein contains Pfa... 29 5.0
>At1g06900.1 68414.m00733 peptidase M16 family protein / insulinase
family protein contains Pfam domain, PF05193: Peptidase
M16 inactive domain; similar to insulin-degrading enzyme
(Insulysin, Insulinase, Insulin protease) [Mouse]
SWISS-PROT:Q9JHR7
Length = 1023
Score = 109 bits (263), Expect = 2e-24
Identities = 47/85 (55%), Positives = 65/85 (76%)
Frame = +1
Query: 508 LGSYSDPAEIQGLAHFVEHMVFMGSEKYPKENEFDAFIKKKGGSDNASTDCEVTTFYFEI 687
+GS+ DP E QGLAHF+EHM+FMGS ++P ENE+D+++ K GGS NA T+ E T ++FE+
Sbjct: 115 MGSFLDPPEAQGLAHFLEHMLFMGSTEFPDENEYDSYLSKHGGSSNAYTEMEHTCYHFEV 174
Query: 688 QEKHLPHAMDMFSQFFVSPLMMKEA 762
+ + L A+ FSQFFV+PLM EA
Sbjct: 175 KREFLQGALKRFSQFFVAPLMKTEA 199
Score = 33.5 bits (73), Expect = 0.18
Identities = 14/21 (66%), Positives = 17/21 (80%)
Frame = +2
Query: 257 KKLYKTIRLENGLTALLISDP 319
++LY+ I LENGL ALLI DP
Sbjct: 21 RRLYRVIELENGLCALLIHDP 41
>At2g41790.1 68415.m05165 peptidase M16 family protein / insulinase
family protein contains Pfam domain, PF05193: Peptidase
M16 inactive domain; similar to insulin-degrading enzyme
(Insulysin, Insulinase, Insulin protease) [Mouse]
SWISS-PROT:Q9JHR7
Length = 970
Score = 96.3 bits (229), Expect = 2e-20
Identities = 40/85 (47%), Positives = 59/85 (69%)
Frame = +1
Query: 508 LGSYSDPAEIQGLAHFVEHMVFMGSEKYPKENEFDAFIKKKGGSDNASTDCEVTTFYFEI 687
+GS+SDP ++GLAHF+EHM+F SEKYP+E+ + +I + GGS NA T E T ++F++
Sbjct: 55 VGSFSDPQGLEGLAHFLEHMLFYASEKYPEEDSYSKYITEHGGSTNAYTASEETNYHFDV 114
Query: 688 QEKHLPHAMDMFSQFFVSPLMMKEA 762
A+D F+QFF+ PLM +A
Sbjct: 115 NADCFDEALDRFAQFFIKPLMSADA 139
>At3g57470.1 68416.m06398 peptidase M16 family protein / insulinase
family protein contains weak similarity to Pfam domain,
PF05193: Peptidase M16 inactive domain
Length = 776
Score = 67.3 bits (157), Expect = 1e-11
Identities = 29/66 (43%), Positives = 42/66 (63%)
Frame = +1
Query: 565 MVFMGSEKYPKENEFDAFIKKKGGSDNASTDCEVTTFYFEIQEKHLPHAMDMFSQFFVSP 744
M+F SEKYP+E+ + +I + GGS NA T E T ++F+I A+D F+QFF+ P
Sbjct: 1 MLFYASEKYPEEDSYSKYITEHGGSTNAYTSSEDTNYHFDINTDSFYEALDRFAQFFIQP 60
Query: 745 LMMKEA 762
LM +A
Sbjct: 61 LMSTDA 66
>At3g02090.2 68416.m00175 mitochondrial processing peptidase beta
subunit, putative similar to mitochondrial processing
peptidase beta subunit, mitochondrial precursor,
Beta-MPP [Human] SWISS-PROT:O75439
Length = 535
Score = 48.0 bits (109), Expect = 8e-06
Identities = 26/83 (31%), Positives = 46/83 (55%)
Frame = +1
Query: 478 KTSSLRPLCWLGSYSDPAEIQGLAHFVEHMVFMGSEKYPKENEFDAFIKKKGGSDNASTD 657
KT+++ GS + E G AHF+EHM+F G+++ + I+ GG NA T
Sbjct: 117 KTATVGVWIDAGSRFESDETNGTAHFLEHMIFKGTDR-RTVRALEEEIEDIGGHLNAYTS 175
Query: 658 CEVTTFYFEIQEKHLPHAMDMFS 726
E TT+Y ++ + ++ A+D+ +
Sbjct: 176 REQTTYYAKVLDSNVNQALDVLA 198
>At3g02090.1 68416.m00174 mitochondrial processing peptidase beta
subunit, putative similar to mitochondrial processing
peptidase beta subunit, mitochondrial precursor,
Beta-MPP [Human] SWISS-PROT:O75439
Length = 531
Score = 48.0 bits (109), Expect = 8e-06
Identities = 26/83 (31%), Positives = 46/83 (55%)
Frame = +1
Query: 478 KTSSLRPLCWLGSYSDPAEIQGLAHFVEHMVFMGSEKYPKENEFDAFIKKKGGSDNASTD 657
KT+++ GS + E G AHF+EHM+F G+++ + I+ GG NA T
Sbjct: 117 KTATVGVWIDAGSRFESDETNGTAHFLEHMIFKGTDR-RTVRALEEEIEDIGGHLNAYTS 175
Query: 658 CEVTTFYFEIQEKHLPHAMDMFS 726
E TT+Y ++ + ++ A+D+ +
Sbjct: 176 REQTTYYAKVLDSNVNQALDVLA 198
>At5g42390.1 68418.m05161 metalloendopeptidase identical to
chloroplast processing enzyme metalloendopeptidase
[Arabidopsis thaliana] gi|2827039|gb|AAC39482
Length = 1265
Score = 38.3 bits (85), Expect = 0.006
Identities = 27/81 (33%), Positives = 37/81 (45%), Gaps = 1/81 (1%)
Frame = +1
Query: 508 LGSYSDPAEIQGLAHFVEHMVFMGSEKYPKENEFDAFIKKKGGSDNASTDCEVTTFYFEI 687
+GS + + QG+AH +EH+ F+GS+K K + G NA TD T F+
Sbjct: 226 VGSIDEEEDEQGIAHMIEHVAFLGSKKREK-------LLGTGARSNAYTDFHHTVFHI-- 276
Query: 688 QEKHLP-HAMDMFSQFFVSPL 747
H P H D F S L
Sbjct: 277 ---HSPTHTKDSEDDLFPSVL 294
>At1g49630.3 68414.m05566 peptidase M16 family protein / insulinase
family protein contains Pfam domain, PF05193: Peptidase
M16 inactive domain
Length = 1080
Score = 33.5 bits (73), Expect = 0.18
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = +1
Query: 526 PAEIQGLAHFVEHMVFMGSEKYPKENEFDAFIK 624
P + G+ H +EH V GS KYP + F +K
Sbjct: 153 PKDSTGIPHILEHSVLCGSRKYPMKEPFVELLK 185
>At1g49630.2 68414.m05565 peptidase M16 family protein / insulinase
family protein contains Pfam domain, PF05193: Peptidase
M16 inactive domain
Length = 1080
Score = 33.5 bits (73), Expect = 0.18
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = +1
Query: 526 PAEIQGLAHFVEHMVFMGSEKYPKENEFDAFIK 624
P + G+ H +EH V GS KYP + F +K
Sbjct: 153 PKDSTGIPHILEHSVLCGSRKYPMKEPFVELLK 185
>At1g49630.1 68414.m05564 peptidase M16 family protein / insulinase
family protein contains Pfam domain, PF05193: Peptidase
M16 inactive domain
Length = 1080
Score = 33.5 bits (73), Expect = 0.18
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = +1
Query: 526 PAEIQGLAHFVEHMVFMGSEKYPKENEFDAFIK 624
P + G+ H +EH V GS KYP + F +K
Sbjct: 153 PKDSTGIPHILEHSVLCGSRKYPMKEPFVELLK 185
>At3g19170.1 68416.m02434 peptidase M16 family protein / insulinase
family protein contains Pfam domain, PF05193: Peptidase
M16 inactive domain
Length = 1080
Score = 33.1 bits (72), Expect = 0.23
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = +1
Query: 526 PAEIQGLAHFVEHMVFMGSEKYPKENEFDAFIK 624
P + G+ H +EH V GS KYP + F +K
Sbjct: 154 PKDSTGIPHILEHSVLCGSRKYPVKEPFVELLK 186
>At4g35240.1 68417.m05009 expressed protein contains Pfam domains,
PF04782: Protein of unknown function (DUF632) and
PF04783: Protein of unknown function (DUF630)
Length = 828
Score = 29.9 bits (64), Expect = 2.2
Identities = 13/47 (27%), Positives = 24/47 (51%)
Frame = +3
Query: 684 DTREAFTSCHGYVQPVLREPANDEGGLQREREAIESEFAIASPSDSN 824
D + + HG + +R +E +QRE +A+E + + +P D N
Sbjct: 719 DRLDTMMTGHGDSEKKVRNMDREEQRIQREIQALEKKMILVAPGDGN 765
>At5g56730.1 68418.m07080 peptidase M16 family protein / insulinase
family protein contains Pfam domain, PF05193: Peptidase
M16 inactive domain
Length = 956
Score = 29.1 bits (62), Expect = 3.8
Identities = 16/59 (27%), Positives = 31/59 (52%), Gaps = 3/59 (5%)
Frame = +1
Query: 508 LGSYSDPAEIQGLAHFVEHMVFMGSEKYPKEN--EF-DAFIKKKGGSDNASTDCEVTTF 675
+GS + + +G+AH VEH+ F + +Y + +F ++ + G NA T + T +
Sbjct: 71 VGSVLEEEDQRGVAHIVEHLAFSATTRYTNHDIVKFLESIGAEFGPCQNAMTTADETIY 129
>At1g53300.1 68414.m06041 thioredoxin family protein contains Pfam
profiles PF00085: Thioredoxin, PF00515: TPR Domain;
similar to tetratricopeptide repeat protein 2
(GI:7248701) [Drosophila melanogaster]; similar to DnaJ
homolog subfamily C member 7 (Tetratricopeptide repeat
protein 2) (TPR repeat protein 2) (Swiss-Prot:Q99615)
[Homo sapiens]
Length = 699
Score = 28.7 bits (61), Expect = 5.0
Identities = 12/29 (41%), Positives = 19/29 (65%)
Frame = +1
Query: 484 SSLRPLCWLGSYSDPAEIQGLAHFVEHMV 570
S+ + LC+LG SDP E+Q L +H++
Sbjct: 313 SARKHLCFLGRPSDPMELQKLEAVEKHLI 341
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,425,939
Number of Sequences: 28952
Number of extensions: 359053
Number of successful extensions: 979
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 951
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 977
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1911862400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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