BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00747 (395 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1685.02c |rps1202|rps12-2|40S ribosomal protein S12|Schizosa... 124 7e-30 SPCC962.04 |rps1201|rps12-1, rps12|40S ribosomal protein S12|Sch... 124 7e-30 SPBC29A3.04 |rpl8||60S ribosomal protein L7a |Schizosaccharomyce... 31 0.065 SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyce... 30 0.11 SPAC694.02 |||DEAD/DEAH box helicase|Schizosaccharomyces pombe|c... 27 1.4 SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|ch... 25 4.3 SPBP23A10.07 |rpa2||DNA-directed RNA polymerase I complex subuni... 25 4.3 SPBC21B10.13c |||transcription factor, homeobox type |Schizosacc... 24 7.4 SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein lig... 24 7.4 SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 24 9.8 SPCC16A11.17 |cdc21|mcm4, SPCC24B10.01|MCM complex subunit Cdc21... 24 9.8 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 24 9.8 SPBC13G1.15c |||dubious|Schizosaccharomyces pombe|chr 2|||Manual 24 9.8 SPAC24H6.01c ||SPAPB21F2.01|membrane bound O-acyltransferase, MB... 24 9.8 >SPBC1685.02c |rps1202|rps12-2|40S ribosomal protein S12|Schizosaccharomyces pombe|chr 2|||Manual Length = 148 Score = 124 bits (298), Expect = 7e-30 Identities = 57/87 (65%), Positives = 70/87 (80%) Frame = +1 Query: 1 ALQEVLKTALIHGGLVHGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNEHQIPL 180 +L+EVLK AL+H GL G+ EA+KALD+RQA LCVL E+CD+ AY KLV+ALC E Q PL Sbjct: 35 SLKEVLKRALVHDGLARGIREASKALDRRQAHLCVLCESCDQEAYVKLVEALCAESQTPL 94 Query: 181 VKVDNNKKLGEWAGLCKIDKDGKARKL 261 VKV + K LGEWAGLC +D+DG ARK+ Sbjct: 95 VKVADPKILGEWAGLCVLDRDGNARKV 121 Score = 36.7 bits (81), Expect = 0.001 Identities = 13/24 (54%), Positives = 19/24 (79%) Frame = +3 Query: 252 KKIVGCSCVVIKDFGEETPALDVL 323 +K+VGCSCV + D+GE++ AL L Sbjct: 119 RKVVGCSCVAVTDYGEDSVALQTL 142 >SPCC962.04 |rps1201|rps12-1, rps12|40S ribosomal protein S12|Schizosaccharomyces pombe|chr 3|||Manual Length = 145 Score = 124 bits (298), Expect = 7e-30 Identities = 56/87 (64%), Positives = 70/87 (80%) Frame = +1 Query: 1 ALQEVLKTALIHGGLVHGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNEHQIPL 180 AL+EVLK AL+H GL G+ EA+KALD+RQA LCVL E+CD+ AY KLV+ALC E + PL Sbjct: 32 ALKEVLKRALVHDGLARGIREASKALDRRQAHLCVLCESCDQEAYVKLVEALCAESETPL 91 Query: 181 VKVDNNKKLGEWAGLCKIDKDGKARKL 261 +KV + K LGEWAGLC +D+DG ARK+ Sbjct: 92 IKVADPKVLGEWAGLCVLDRDGNARKV 118 Score = 36.3 bits (80), Expect = 0.002 Identities = 13/24 (54%), Positives = 19/24 (79%) Frame = +3 Query: 252 KKIVGCSCVVIKDFGEETPALDVL 323 +K+VGCSCV + D+GE++ AL L Sbjct: 116 RKVVGCSCVAVTDYGEDSAALQKL 139 >SPBC29A3.04 |rpl8||60S ribosomal protein L7a |Schizosaccharomyces pombe|chr 2|||Manual Length = 259 Score = 31.1 bits (67), Expect = 0.065 Identities = 14/54 (25%), Positives = 27/54 (50%) Frame = +1 Query: 49 HGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNEHQIPLVKVDNNKKLG 210 +GL+ ++ ++A L ++A + D + ALC + +P V N +LG Sbjct: 134 YGLNHVVALIEAKKAKLVLIASDVDPIELVVFLPALCKKMGVPYAIVKNKARLG 187 >SPAC1782.10c |nhp2||RNA-binding protein Nhp2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 154 Score = 30.3 bits (65), Expect = 0.11 Identities = 16/70 (22%), Positives = 33/70 (47%) Frame = +1 Query: 4 LQEVLKTALIHGGLVHGLHEAAKALDKRQAVLCVLAENCDEAAYKKLVQALCNEHQIPLV 183 + + +K A ++ G+ E KA+ K + L +LA + + LC ++ +P + Sbjct: 41 MMKTVKKASKQKHILRGVKEVVKAVRKGEKGLVILAGDISPMDVISHIPVLCEDNNVPYL 100 Query: 184 KVDNNKKLGE 213 + + LGE Sbjct: 101 YTVSKELLGE 110 >SPAC694.02 |||DEAD/DEAH box helicase|Schizosaccharomyces pombe|chr 1|||Manual Length = 1717 Score = 26.6 bits (56), Expect = 1.4 Identities = 20/59 (33%), Positives = 31/59 (52%) Frame = +1 Query: 136 KKLVQALCNEHQIPLVKVDNNKKLGEWAGLCKIDKDGKARKLSAAPVLSSKISARKLQR 312 K+ QA+ E + N K G+ A K K KA KLS A + ++I+ARK+++ Sbjct: 507 KESKQAVKEETSVKNSPKGKNTKNGK-AEKEKPGKKSKAPKLSKAEQIRAEIAARKVEK 564 >SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|chr 3|||Manual Length = 923 Score = 25.0 bits (52), Expect = 4.3 Identities = 10/35 (28%), Positives = 19/35 (54%) Frame = +2 Query: 107 WLRTVMKPHTRNLFRLCATNIRFHWSRLTTTKSLE 211 ++ +V P L+RLC++ R W ++SL+ Sbjct: 220 FILSVFHPDAARLYRLCSSLKRICWELYDPSRSLD 254 >SPBP23A10.07 |rpa2||DNA-directed RNA polymerase I complex subunit Rpa2|Schizosaccharomyces pombe|chr 2|||Manual Length = 1227 Score = 25.0 bits (52), Expect = 4.3 Identities = 12/47 (25%), Positives = 25/47 (53%) Frame = +1 Query: 187 VDNNKKLGEWAGLCKIDKDGKARKLSAAPVLSSKISARKLQRWMCSR 327 VD + LG G + + +++ +P++ K S+R Q+ +CS+ Sbjct: 921 VDEVRLLGNDVGDSECQQIHVKLRITRSPIIGDKFSSRHGQKGICSQ 967 >SPBC21B10.13c |||transcription factor, homeobox type |Schizosaccharomyces pombe|chr 2|||Manual Length = 201 Score = 24.2 bits (50), Expect = 7.4 Identities = 12/41 (29%), Positives = 20/41 (48%) Frame = +1 Query: 229 KIDKDGKARKLSAAPVLSSKISARKLQRWMCSRTTSSRRAN 351 K K + + L S ++ R+LQ W ++ S RR+N Sbjct: 54 KTPKPSLIERQELSKKLKSSMTPRELQIWFQNKRQSLRRSN 94 >SPAC12B10.01c ||SPAC31F12.02c, SPAC637.15c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1647 Score = 24.2 bits (50), Expect = 7.4 Identities = 13/34 (38%), Positives = 17/34 (50%), Gaps = 1/34 (2%) Frame = +1 Query: 28 LIHGGLVHGLHEAAKALDKRQAVLCVLAEN-CDE 126 L+H GL+H L + K +LA N CDE Sbjct: 896 LLHSGLIHNLLLSLKKFGSSSLRTFLLAMNTCDE 929 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 23.8 bits (49), Expect = 9.8 Identities = 14/45 (31%), Positives = 21/45 (46%) Frame = -2 Query: 136 CMRLHHSSQPKHIVLLASCQEL*RLREDRAQDHHGLKLSLAPLAV 2 C+ S +HIV A L +D +DH G+ L ++P V Sbjct: 517 CVLCTTSESGEHIVAGAGELHLEICLKDLQEDHAGIPLKISPPVV 561 >SPCC16A11.17 |cdc21|mcm4, SPCC24B10.01|MCM complex subunit Cdc21|Schizosaccharomyces pombe|chr 3|||Manual Length = 911 Score = 23.8 bits (49), Expect = 9.8 Identities = 10/32 (31%), Positives = 14/32 (43%) Frame = -2 Query: 307 GVSSPKSLMTTQEQPTIFLPCHPCQSCRDQPI 212 GVS+P SL+ + F HP D + Sbjct: 125 GVSTPSSLLFSGSDALTFSQAHPSSEVADDTV 156 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 23.8 bits (49), Expect = 9.8 Identities = 14/45 (31%), Positives = 21/45 (46%) Frame = -2 Query: 136 CMRLHHSSQPKHIVLLASCQEL*RLREDRAQDHHGLKLSLAPLAV 2 C+ S +HIV A L +D +DH G+ L ++P V Sbjct: 517 CVLCTTSESGEHIVAGAGELHLEICLKDLQEDHAGIPLKISPPVV 561 >SPBC13G1.15c |||dubious|Schizosaccharomyces pombe|chr 2|||Manual Length = 111 Score = 23.8 bits (49), Expect = 9.8 Identities = 10/22 (45%), Positives = 13/22 (59%) Frame = -3 Query: 105 NT*YCLPLVKSFSGFVKTVHKT 40 N YCLP +F FVK + +T Sbjct: 90 NRLYCLPFCNAFIEFVKKLTET 111 >SPAC24H6.01c ||SPAPB21F2.01|membrane bound O-acyltransferase, MBOAT |Schizosaccharomyces pombe|chr 1|||Manual Length = 588 Score = 23.8 bits (49), Expect = 9.8 Identities = 6/14 (42%), Positives = 11/14 (78%) Frame = -2 Query: 142 VSCMRLHHSSQPKH 101 ++C ++H+ S PKH Sbjct: 72 IACFKIHYVSSPKH 85 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,552,772 Number of Sequences: 5004 Number of extensions: 28390 Number of successful extensions: 99 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 96 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 99 length of database: 2,362,478 effective HSP length: 66 effective length of database: 2,032,214 effective search space used: 132093910 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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