Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00727
(597 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC25H2.02 |ths1||threonine-tRNA ligase Ths1 |Schizosaccharomyc... 144 7e-36
SPAC24C9.09 |||mitochondrial threonine-tRNA ligase|Schizosacchar... 103 1e-23
SPAC926.09c |fas1||fatty acid synthase beta subunit Fas1|Schizos... 27 2.7
SPBC24C6.03 |||proline-tRNA ligase |Schizosaccharomyces pombe|ch... 27 2.7
SPBC56F2.05c |||transcription factor |Schizosaccharomyces pombe|... 27 2.7
SPBC3B9.22c |dad4||DASH complex subunit Dad4|Schizosaccharomyces... 26 4.8
SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces po... 25 6.3
SPBC106.03 |||DUF1776 family protein|Schizosaccharomyces pombe|c... 25 6.3
SPBP8B7.21 |ubp3||ubiquitin C-terminal hydrolase Ubp3|Schizosacc... 25 6.3
SPAC31A2.11c |cuf1||Cu metalloregulatory transcription factor Cu... 25 6.3
SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1 |Schi... 25 6.3
SPAC17G6.05c |||Rhophilin-2 homolog|Schizosaccharomyces pombe|ch... 25 6.3
SPBC543.06c |dbp8||ATP-dependent RNA helicase Dbp8 |Schizosaccha... 25 8.4
SPAC890.03 |ppk16|mug92|serine/threonine protein kinase Ppk16 |S... 25 8.4
>SPBC25H2.02 |ths1||threonine-tRNA ligase Ths1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 703
Score = 144 bits (350), Expect = 7e-36
Identities = 57/85 (67%), Positives = 73/85 (85%)
Frame = +2
Query: 254 YNTLVNFIKEQYRSRGFHEVVTPNMYNAKLWQTSGHWAHYAENMFSFDVEKETFALKPMN 433
YNTL+ +++ QY RG+ EV+TPNMYN LW+TSGHW +Y+ENMFSFD+EKE +ALKPMN
Sbjct: 321 YNTLLKYMRYQYSKRGYQEVITPNMYNVNLWKTSGHWNNYSENMFSFDIEKEKYALKPMN 380
Query: 434 CPGHCLMFDNRTRSWRELPLRLADF 508
CPGHC+MF +R RS+R+LP R+ADF
Sbjct: 381 CPGHCVMFKSRDRSYRDLPWRVADF 405
Score = 103 bits (247), Expect = 2e-23
Identities = 45/66 (68%), Positives = 56/66 (84%)
Frame = +3
Query: 57 GQSGSESLQRVYGISFPEPKQLKEWEVIQEEAAKRDHRKIGREQELFFFHELSPGSCFFQ 236
G + ++SLQRVYGISFP+ KQ++E++ EAAKRDHRKIGR+QELFFF+E+SPGSCFF
Sbjct: 255 GDAKNDSLQRVYGISFPDNKQMQEYKTFLAEAAKRDHRKIGRDQELFFFNEISPGSCFFL 314
Query: 237 PRGAHI 254
P GA I
Sbjct: 315 PHGARI 320
Score = 62.1 bits (144), Expect = 6e-11
Identities = 27/29 (93%), Positives = 28/29 (96%)
Frame = +1
Query: 511 VLHRNELSGALTGLTRVRRFQQDDAHIFC 597
VLHRNE SGAL+GLTRVRRFQQDDAHIFC
Sbjct: 407 VLHRNEFSGALSGLTRVRRFQQDDAHIFC 435
Score = 32.3 bits (70), Expect = 0.055
Identities = 12/23 (52%), Positives = 19/23 (82%)
Frame = +1
Query: 1 HTGKVKALKVTKNSATYWEGKAE 69
HTG++K+ VTKNS++Y+ G A+
Sbjct: 236 HTGRIKSFAVTKNSSSYFLGDAK 258
>SPAC24C9.09 |||mitochondrial threonine-tRNA
ligase|Schizosaccharomyces pombe|chr 1|||Manual
Length = 473
Score = 103 bits (248), Expect = 1e-23
Identities = 49/108 (45%), Positives = 71/108 (65%), Gaps = 8/108 (7%)
Frame = +2
Query: 209 TVSRLVFLPATWSSHYNTLVNFIKEQYRSRGFHEVVTPNMYNAKLWQTSGHWAHYAENMF 388
T + FLP + YN LV+F++ QY+ GF E++TP ++ LW+ SGHW +Y + +F
Sbjct: 64 TPGSIFFLPHG-TRIYNRLVDFLRAQYQIHGFEEIITPLIFKKDLWEKSGHWQNYEKEIF 122
Query: 389 ----SFDVEKE----TFALKPMNCPGHCLMFDNRTRSWRELPLRLADF 508
S +VE E T+ LKPMNCPGHC+++ + RS++ELPLR ADF
Sbjct: 123 RVEESRNVEDEHSNATYGLKPMNCPGHCIVYASTERSYKELPLRFADF 170
Score = 52.0 bits (119), Expect = 6e-08
Identities = 22/28 (78%), Positives = 24/28 (85%)
Frame = +1
Query: 514 LHRNELSGALTGLTRVRRFQQDDAHIFC 597
LHRNE SGAL+GLTR+R F QDD HIFC
Sbjct: 173 LHRNEASGALSGLTRLRCFHQDDGHIFC 200
Score = 40.3 bits (90), Expect = 2e-04
Identities = 22/57 (38%), Positives = 28/57 (49%), Gaps = 1/57 (1%)
Frame = +3
Query: 87 VYGISF-PEPKQLKEWEVIQEEAAKRDHRKIGREQELFFFHELSPGSCFFQPRGAHI 254
+Y F P P K + Q E+ DHR I Q+L+ L+PGS FF P G I
Sbjct: 21 IYTARFGPTPFSTKANNLKQPESTLNDHRTIAARQKLYTTSILTPGSIFFLPHGTRI 77
>SPAC926.09c |fas1||fatty acid synthase beta subunit
Fas1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2073
Score = 26.6 bits (56), Expect = 2.7
Identities = 15/43 (34%), Positives = 20/43 (46%), Gaps = 3/43 (6%)
Frame = +1
Query: 151 PPSVTTERL---EGNKSYSSSTNCLPARVSSSHVELTLQHTRE 270
PPSV + L EGN + + LP + H+E T H E
Sbjct: 316 PPSVVADSLASSEGNPTPMLAVRDLPIETLNKHIETTNTHLPE 358
>SPBC24C6.03 |||proline-tRNA ligase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 425
Score = 26.6 bits (56), Expect = 2.7
Identities = 16/82 (19%), Positives = 32/82 (39%), Gaps = 1/82 (1%)
Frame = +2
Query: 257 NTLVNFIKEQYRSRGFHEVVTPNMYNAKLWQTSGHWAHYAENMFSF-DVEKETFALKPMN 433
N + + + RS G + ++ + ++W+ SG W +F D L P +
Sbjct: 56 NKICDLLHISMRSLGASAISLAHLSSKEIWEKSGRWQKTGSELFRLHDRNDREMCLAPTH 115
Query: 434 CPGHCLMFDNRTRSWRELPLRL 499
S ++LP+R+
Sbjct: 116 EEDVTRTMATIIDSQKQLPIRV 137
>SPBC56F2.05c |||transcription factor |Schizosaccharomyces pombe|chr
2|||Manual
Length = 397
Score = 26.6 bits (56), Expect = 2.7
Identities = 24/75 (32%), Positives = 34/75 (45%)
Frame = +1
Query: 22 LKVTKNSATYWEGKAEARAYSACTVYHFQNRNS*KNGRSFKRRPPSVTTERLEGNKSYSS 201
+K TK S K E R+ F N +S + + +RRP T L+ + S +S
Sbjct: 242 VKPTKASGVVKSEKVEKRSLPP---QDFGNASSSTSAK--RRRPDHNHTSTLDASSSNTS 296
Query: 202 STNCLPARVSSSHVE 246
+ P VSSS VE
Sbjct: 297 LASTGPMTVSSSTVE 311
>SPBC3B9.22c |dad4||DASH complex subunit Dad4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 72
Score = 25.8 bits (54), Expect = 4.8
Identities = 16/40 (40%), Positives = 21/40 (52%)
Frame = +2
Query: 305 HEVVTPNMYNAKLWQTSGHWAHYAENMFSFDVEKETFALK 424
H + NM N + S WA+YA N+ F +E ET LK
Sbjct: 32 HSLQLINMSNMNVELASQMWANYARNV-KFHLE-ETHTLK 69
>SPCC23B6.03c |tel1||ATM checkpoint kinase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 2812
Score = 25.4 bits (53), Expect = 6.3
Identities = 11/37 (29%), Positives = 19/37 (51%)
Frame = +2
Query: 176 WKGTRVILLPRTVSRLVFLPATWSSHYNTLVNFIKEQ 286
WK VI + + V L+++ + Y +NF+ EQ
Sbjct: 191 WKSHDVIRVKQEVVELIYVMRSLVQWYAAPINFVSEQ 227
>SPBC106.03 |||DUF1776 family protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 357
Score = 25.4 bits (53), Expect = 6.3
Identities = 11/24 (45%), Positives = 14/24 (58%)
Frame = +1
Query: 151 PPSVTTERLEGNKSYSSSTNCLPA 222
PPS+ TER NK + S C P+
Sbjct: 289 PPSLCTERKVLNKIFDSLLGCWPS 312
>SPBP8B7.21 |ubp3||ubiquitin C-terminal hydrolase
Ubp3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 512
Score = 25.4 bits (53), Expect = 6.3
Identities = 12/28 (42%), Positives = 17/28 (60%), Gaps = 4/28 (14%)
Frame = -3
Query: 247 APRGW----KKHEPGDSSWKKNNSCSLP 176
+P+ W KKH GDS KK++S +P
Sbjct: 73 SPKSWSAIAKKHVQGDSPVKKSHSVPVP 100
>SPAC31A2.11c |cuf1||Cu metalloregulatory transcription factor Cuf1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 411
Score = 25.4 bits (53), Expect = 6.3
Identities = 15/44 (34%), Positives = 22/44 (50%), Gaps = 4/44 (9%)
Frame = -3
Query: 214 DSSWKKNNSCSLPIFLWSRLAASS*MTSH----SFSCFGSGNDI 95
DSS N+SCS+P + +L + +H S SC G D+
Sbjct: 79 DSSSASNSSCSIPSSISEKLLPRDNVKTHCPKRSASCCGKKPDV 122
>SPBC30B4.01c |wsc1|SPBC3D6.14c|transmembrane receptor Wsc1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 374
Score = 25.4 bits (53), Expect = 6.3
Identities = 19/76 (25%), Positives = 33/76 (43%)
Frame = -2
Query: 542 SAPLSSLRCSTRSRPTEAATRATTSCGCRTSGNVRDSSSVSKRTFPFQRRMRTCFQHSEP 363
S+ S+ S+ S P+ ++T TTS +S + SSS S + + S
Sbjct: 130 SSVSSTTSSSSSSSPSSSSTTTTTSPSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS 189
Query: 362 SDPRSATAWRCTCSGS 315
S S+++ + S S
Sbjct: 190 SSSSSSSSSSSSSSSS 205
>SPAC17G6.05c |||Rhophilin-2 homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 775
Score = 25.4 bits (53), Expect = 6.3
Identities = 12/45 (26%), Positives = 23/45 (51%)
Frame = +3
Query: 78 LQRVYGISFPEPKQLKEWEVIQEEAAKRDHRKIGREQELFFFHEL 212
+ R+YG S Q+K + ++E AA D G++ ++ +L
Sbjct: 29 VSRIYGNSVDVEDQIKAFNTLRENAADVDDTVAGKDILYSYYGQL 73
>SPBC543.06c |dbp8||ATP-dependent RNA helicase Dbp8
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 453
Score = 25.0 bits (52), Expect = 8.4
Identities = 16/53 (30%), Positives = 28/53 (52%)
Frame = +1
Query: 427 DELSRTLPDVRQPHEVVARVAASVGRLLVLHRNELSGALTGLTRVRRFQQDDA 585
D + +++ ++PH VVA + GRL L R+ + GL R++ D+A
Sbjct: 114 DMIRQSIDLSKRPHVVVA----TPGRLADLIRSNGEETIAGLRRIKFLVMDEA 162
>SPAC890.03 |ppk16|mug92|serine/threonine protein kinase Ppk16
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 672
Score = 25.0 bits (52), Expect = 8.4
Identities = 10/35 (28%), Positives = 20/35 (57%)
Frame = +1
Query: 151 PPSVTTERLEGNKSYSSSTNCLPARVSSSHVELTL 255
PP + T L+GNK ++ +N + SS++ + +
Sbjct: 490 PPLLKTVNLDGNKDFTFPSNSQNSPSKSSNLSINI 524
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,451,658
Number of Sequences: 5004
Number of extensions: 53806
Number of successful extensions: 210
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 190
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 209
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 260219058
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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