Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00727
(597 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g26830.1 68418.m03201 threonyl-tRNA synthetase / threonine--t... 138 2e-33
At2g04842.1 68415.m00498 threonyl-tRNA synthetase, putative / th... 75 3e-14
At1g17960.1 68414.m02222 threonyl-tRNA synthetase, putative / th... 56 1e-08
At4g16760.1 68417.m02531 acyl-CoA oxidase (ACX1) identical to ac... 33 0.11
At2g41570.1 68415.m05137 hypothetical protein similar to zinc fi... 31 0.77
At3g46070.1 68416.m04985 zinc finger (C2H2 type) family protein ... 28 4.1
At1g20760.1 68414.m02600 calcium-binding EF hand family protein ... 28 4.1
At2g20340.1 68415.m02375 tyrosine decarboxylase, putative simila... 28 5.4
At1g07530.1 68414.m00806 scarecrow-like transcription factor 14 ... 28 5.4
At3g46130.1 68416.m04992 myb family transcription factor (MYB48)... 27 7.2
At2g29860.1 68415.m03626 kelch repeat-containing F-box family pr... 27 7.2
At1g71697.1 68414.m08288 choline kinase, putative similar to GmC... 27 9.5
>At5g26830.1 68418.m03201 threonyl-tRNA synthetase / threonine--tRNA
ligase (THRRS) identical to SP|O04630 Threonyl-tRNA
synthetase, mitochondrial precursor (EC 6.1.1.3)
(Threonine--tRNA ligase) (ThrRS) {Arabidopsis thaliana}
Length = 709
Score = 138 bits (335), Expect = 2e-33
Identities = 57/85 (67%), Positives = 72/85 (84%)
Frame = +2
Query: 254 YNTLVNFIKEQYRSRGFHEVVTPNMYNAKLWQTSGHWAHYAENMFSFDVEKETFALKPMN 433
YN L++FIK QY RG+ EV+TPNMYN +LWQTSGH +Y +NMF+F++EK+ F LKPMN
Sbjct: 347 YNRLMDFIKNQYWHRGYTEVITPNMYNMELWQTSGHADNYKDNMFTFNIEKQEFGLKPMN 406
Query: 434 CPGHCLMFDNRTRSWRELPLRLADF 508
CPGHCL+F +R RS+RELP+RLADF
Sbjct: 407 CPGHCLIFQHRVRSYRELPMRLADF 431
Score = 85.4 bits (202), Expect = 3e-17
Identities = 39/66 (59%), Positives = 48/66 (72%)
Frame = +3
Query: 57 GQSGSESLQRVYGISFPEPKQLKEWEVIQEEAAKRDHRKIGREQELFFFHELSPGSCFFQ 236
G ESLQRVYGIS+P+ KQLK++ EEA K DHR +G++QELFF H+LSPGS FF
Sbjct: 281 GDKDRESLQRVYGISYPDQKQLKKYLQFLEEAKKYDHRLLGQKQELFFSHQLSPGSYFFL 340
Query: 237 PRGAHI 254
P G +
Sbjct: 341 PLGTRV 346
Score = 61.7 bits (143), Expect = 4e-10
Identities = 27/29 (93%), Positives = 28/29 (96%)
Frame = +1
Query: 511 VLHRNELSGALTGLTRVRRFQQDDAHIFC 597
VLHRNE SGAL+GLTRVRRFQQDDAHIFC
Sbjct: 433 VLHRNEASGALSGLTRVRRFQQDDAHIFC 461
>At2g04842.1 68415.m00498 threonyl-tRNA synthetase, putative /
threonine--tRNA ligase, putative similar to SP|P18256
Threonyl-tRNA synthetase 2 (EC 6.1.1.3) (Threonine--tRNA
ligase) (ThrRS) {Bacillus subtilis}; contains Pfam
profiles PF00587: tRNA synthetase class II core domain
(G, H, P, S and T), PF03129: Anticodon binding domain
Length = 650
Score = 75.4 bits (177), Expect = 3e-14
Identities = 30/77 (38%), Positives = 51/77 (66%), Gaps = 1/77 (1%)
Frame = +2
Query: 278 KEQYRSRGFHEVVTPNMYNAKLWQTSGHWAHYAENMFS-FDVEKETFALKPMNCPGHCLM 454
K+ + G+ + TP++ A LW+ SGH Y ENM+ ++E E + L+PMNCP H L+
Sbjct: 294 KKMHVEHGYDLIYTPHVAKADLWKISGHLDFYRENMYDQMEIEDELYQLRPMNCPYHILL 353
Query: 455 FDNRTRSWRELPLRLAD 505
+ + +S+R+LP+R+A+
Sbjct: 354 YQRKRQSYRDLPIRVAE 370
Score = 55.2 bits (127), Expect = 3e-08
Identities = 31/80 (38%), Positives = 46/80 (57%), Gaps = 5/80 (6%)
Frame = +3
Query: 24 KSYEELGNVLG----GQSGSESLQRVYGISFPEPKQLKEWEVIQEEAAKRDHRKIGREQE 191
K EL +V G G + LQR+YG ++ +QLK + +EEA +RDHR+IG++ +
Sbjct: 204 KKAVELESVAGAYWRGDEKRQMLQRIYGTAWESEEQLKAYLHFKEEAKRRDHRRIGQDLD 263
Query: 192 LFFFH-ELSPGSCFFQPRGA 248
LF E G F+ P+GA
Sbjct: 264 LFSIQDEAGGGLVFWHPKGA 283
Score = 46.4 bits (105), Expect = 1e-05
Identities = 22/34 (64%), Positives = 25/34 (73%)
Frame = +1
Query: 496 VGRLLVLHRNELSGALTGLTRVRRFQQDDAHIFC 597
V L ++R ELSG+L GL RVR F QDDAHIFC
Sbjct: 368 VAELGTVYRYELSGSLHGLFRVRGFTQDDAHIFC 401
>At1g17960.1 68414.m02222 threonyl-tRNA synthetase, putative /
threonine--tRNA ligase, putative similar to SP|O04630
Threonyl-tRNA synthetase, mitochondrial precursor (EC
6.1.1.3) (Threonine--tRNA ligase) (ThrRS) {Arabidopsis
thaliana}; contains Pfam profiles PF00587: tRNA
synthetase class II core domain (G, H, P, S and T),
PF03129: Anticodon binding domain, PF02824: TGS domain
Length = 458
Score = 56.4 bits (130), Expect = 1e-08
Identities = 26/42 (61%), Positives = 31/42 (73%)
Frame = +1
Query: 472 VVARVAASVGRLLVLHRNELSGALTGLTRVRRFQQDDAHIFC 597
+ A++ + VLHRNE SGAL G+TRVRRF QDDAHIFC
Sbjct: 166 IEAKLPIRMAEFGVLHRNEDSGALGGMTRVRRFVQDDAHIFC 207
>At4g16760.1 68417.m02531 acyl-CoA oxidase (ACX1) identical to
acyl-CoA oxidase [Arabidopsis thaliana] GI:3044214
Length = 675
Score = 33.5 bits (73), Expect = 0.11
Identities = 23/57 (40%), Positives = 34/57 (59%), Gaps = 2/57 (3%)
Frame = -1
Query: 594 EDVRVVLLEAPHAREAGERAAQLVAVQ--HEKSANRSGNSRHDLVRLSNIRQCPGQF 430
ED+++V + HA E +R A+LVA EKS NR+ SR +L + S +R+C F
Sbjct: 19 EDMKIVWAGSRHAFEVSDRIARLVASDPVFEKS-NRARLSRKELFK-STLRKCAHAF 73
>At2g41570.1 68415.m05137 hypothetical protein similar to zinc
finger protein [Arabidopsis thaliana] GI:976277
Length = 418
Score = 30.7 bits (66), Expect = 0.77
Identities = 13/28 (46%), Positives = 19/28 (67%)
Frame = +1
Query: 154 PSVTTERLEGNKSYSSSTNCLPARVSSS 237
PS +R+EGN + S+ TN L +R+ SS
Sbjct: 257 PSSDLDRIEGNSALSTKTNLLNSRIMSS 284
>At3g46070.1 68416.m04985 zinc finger (C2H2 type) family protein
contains zinc finger, C2H2 type, domain, PROSITE:PS00028
Length = 170
Score = 28.3 bits (60), Expect = 4.1
Identities = 18/64 (28%), Positives = 29/64 (45%)
Frame = +3
Query: 177 GREQELFFFHELSPGSCFFQPRGAHITTHS*ISLKNNTGLEDSMKW*PRTCTTPSCGRPR 356
GR++ +F FQ G H +HS ++ ++ L S K P+T TT +
Sbjct: 30 GRKKRVFRCKTCERDFDSFQALGGHRASHSKLTNSDDKSLPGSPKKKPKTTTTTTAHTCP 89
Query: 357 VTGL 368
+ GL
Sbjct: 90 ICGL 93
>At1g20760.1 68414.m02600 calcium-binding EF hand family protein
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain
Length = 1019
Score = 28.3 bits (60), Expect = 4.1
Identities = 14/34 (41%), Positives = 18/34 (52%)
Frame = -2
Query: 449 GNVRDSSSVSKRTFPFQRRMRTCFQHSEPSDPRS 348
GN + S S R+F QR+ + F S PS P S
Sbjct: 840 GNTARADSPSSRSFGAQRKSQFAFDDSVPSTPLS 873
>At2g20340.1 68415.m02375 tyrosine decarboxylase, putative similar
to tyrosine/dopa decarboxylase [Papaver somniferum]
GI:3282527, SP|Q06087 Tyrosine decarboxylase 3 (EC
4.1.1.25) {Petroselinum crispum}; contains Pfam profile
PF00282: Pyridoxal-dependent decarboxylase conserved
domain
Length = 490
Score = 27.9 bits (59), Expect = 5.4
Identities = 15/37 (40%), Positives = 21/37 (56%), Gaps = 1/37 (2%)
Frame = +3
Query: 63 SGSESLQRVYGISFPEPKQLKE-WEVIQEEAAKRDHR 170
SG L+ G E K +KE W++IQEEA+ H+
Sbjct: 454 SGKIVLRCAIGAPLTEEKHVKEAWKIIQEEASYLLHK 490
>At1g07530.1 68414.m00806 scarecrow-like transcription factor 14
(SCL14) identical to GB:AAD24412 from [Arabidopsis
thaliana] (Plant J. 18 (1), 111-119 (1999))
Length = 769
Score = 27.9 bits (59), Expect = 5.4
Identities = 17/51 (33%), Positives = 27/51 (52%)
Frame = +2
Query: 191 VILLPRTVSRLVFLPATWSSHYNTLVNFIKEQYRSRGFHEVVTPNMYNAKL 343
V+ L R ++ VF+PA S +YN F ++R FH +M ++KL
Sbjct: 618 VLKLIRKINPNVFIPAILSGNYN--APFFVTRFREALFHYSAVFDMCDSKL 666
>At3g46130.1 68416.m04992 myb family transcription factor (MYB48)
contains Pfam profile: PF00249 myb-like DNA-binding
domain
Length = 256
Score = 27.5 bits (58), Expect = 7.2
Identities = 17/44 (38%), Positives = 21/44 (47%), Gaps = 1/44 (2%)
Frame = -2
Query: 569 KRRTRVRPVSAPLSSLRCSTRSRPTEAATRATTSCGCR-TSGNV 441
K + + RPVS S CS+ S T TSC R +SG V
Sbjct: 110 KAQEKKRPVSPTSSFSNCSSSSVTTTTTNTQDTSCHSRKSSGEV 153
>At2g29860.1 68415.m03626 kelch repeat-containing F-box family
protein contains Pfam profiles PF01344: Kelch motif,
PF00646: F-box domain
Length = 240
Score = 27.5 bits (58), Expect = 7.2
Identities = 14/57 (24%), Positives = 30/57 (52%)
Frame = +2
Query: 269 NFIKEQYRSRGFHEVVTPNMYNAKLWQTSGHWAHYAENMFSFDVEKETFALKPMNCP 439
+++++ R+R +H TP + + +++ G A+ + FDV E++ P CP
Sbjct: 146 HYLQDMQRAR-YH--ATPGVIDGRIYVIGGRKKQDADWVEVFDVTTESWETVPTQCP 199
>At1g71697.1 68414.m08288 choline kinase, putative similar to GmCK2p
choline kinase gi|1438881|gb|AAC49375
Length = 346
Score = 27.1 bits (57), Expect = 9.5
Identities = 13/43 (30%), Positives = 22/43 (51%)
Frame = +2
Query: 296 RGFHEVVTPNMYNAKLWQTSGHWAHYAENMFSFDVEKETFALK 424
R FH++ P N LW+ W A+N+ S +E + + L+
Sbjct: 141 REFHKLDMPGPKNVLLWERLRTWLKEAKNLAS-PIEMDKYRLE 182
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,203,445
Number of Sequences: 28952
Number of extensions: 296999
Number of successful extensions: 963
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 925
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 962
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1190791976
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -