Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00723
(720 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g44050.1 68416.m04718 kinesin motor protein-related KLP2 prot... 34 0.11
At3g30550.1 68416.m03866 hypothetical protein includes At2g05890... 32 0.33
At3g27710.1 68416.m03460 zinc finger protein-related contains si... 31 0.77
At4g39510.1 68417.m05587 cytochrome P450 family protein contains... 30 1.8
At3g27720.1 68416.m03461 zinc finger protein-related contains Pf... 30 1.8
At2g17260.1 68415.m01993 glutamate receptor family protein (GLR3... 30 1.8
At3g59690.1 68416.m06660 calmodulin-binding family protein simil... 29 2.3
At5g63450.1 68418.m07965 cytochrome P450, putative 28 5.4
At1g29510.1 68414.m03610 auxin-responsive protein, putative simi... 28 5.4
At4g35290.2 68417.m05015 glutamate receptor family protein (GLR3... 28 7.2
At4g35290.1 68417.m05014 glutamate receptor family protein (GLR3... 28 7.2
At4g35000.1 68417.m04963 L-ascorbate peroxidase 3 (APX3) identic... 28 7.2
At4g13040.1 68417.m02035 AP2 domain-containing transcription fac... 28 7.2
At5g57180.2 68418.m07143 expressed protein ; supporting cDNA gi|... 27 9.5
At5g57180.1 68418.m07142 expressed protein ; supporting cDNA gi|... 27 9.5
At5g47730.1 68418.m05897 SEC14 cytosolic factor, putative / poly... 27 9.5
At4g00620.1 68417.m00086 tetrahydrofolate dehydrogenase/cyclohyd... 27 9.5
At3g13540.1 68416.m01702 myb family transcription factor contain... 27 9.5
At2g32390.1 68415.m03958 glutamate receptor family protein (GLR3... 27 9.5
At1g02640.1 68414.m00214 glycosyl hydrolase family 3 protein sim... 27 9.5
>At3g44050.1 68416.m04718 kinesin motor protein-related KLP2
protein, Xenopus laevis, PIR:T30335
Length = 1229
Score = 33.9 bits (74), Expect = 0.11
Identities = 27/91 (29%), Positives = 42/91 (46%), Gaps = 5/91 (5%)
Frame = +3
Query: 6 KMKT-VQVILCLFVASLYANETSVSDSKLEDDLYNSI--LVADYDHSVEKSKQIYEDKKS 176
++KT VQ I C+ A+ET++ SK DDL I L+ D + +E +Q+ E+ S
Sbjct: 714 EVKTMVQAIACVSQREAEAHETAIKLSKENDDLRQKIKVLIEDNNKLIELYEQVAEENSS 773
Query: 177 EVITNVVNKLIRNN--KMNCMEYAYQLWSKA 263
+ NN N E A ++ A
Sbjct: 774 RAWGKIETDSSSNNADAQNSAEIALEVEKSA 804
>At3g30550.1 68416.m03866 hypothetical protein includes At2g05890,
At4g07450, At3g30630, At3g43100, At2g09960, At3g30550,
At1g39430, At2g10460, At4g03640, At5g35250
Length = 509
Score = 32.3 bits (70), Expect = 0.33
Identities = 19/66 (28%), Positives = 38/66 (57%), Gaps = 1/66 (1%)
Frame = +3
Query: 6 KMKTVQVILCLFVASLYANETSVSDSKL-EDDLYNSILVADYDHSVEKSKQIYEDKKSEV 182
K KT Q +LC+ +++ S + +++ D++ + I+ DYD V+ S I ++ S +
Sbjct: 398 KGKTFQFLLCVQRENIFGGYDSFTVARVYTDNIADEIVQEDYDAYVDPSSLISIEQDSLM 457
Query: 183 ITNVVN 200
+TN V+
Sbjct: 458 LTNGVD 463
>At3g27710.1 68416.m03460 zinc finger protein-related contains
similarity to zinc finger proteins and Pfam domain,
PF01485: IBR domain
Length = 537
Score = 31.1 bits (67), Expect = 0.77
Identities = 17/67 (25%), Positives = 33/67 (49%)
Frame = +3
Query: 54 YANETSVSDSKLEDDLYNSILVADYDHSVEKSKQIYEDKKSEVITNVVNKLIRNNKMNCM 233
Y + + KLED L SIL +S K ++++ K+ +T+ VN+L + ++
Sbjct: 353 YRYKAHIDSLKLEDKLRKSILEKAVSNSETKDQKVF--KEYSWVTDAVNRLFISRRILSQ 410
Query: 234 EYAYQLW 254
Y + +
Sbjct: 411 SYPFAFY 417
>At4g39510.1 68417.m05587 cytochrome P450 family protein contains
Pfam PF00067: Cytochrome P450; similar to Cytochrome
P450 86A2 (SP:O23066) [Arabidopsis thaliana]
Length = 508
Score = 29.9 bits (64), Expect = 1.8
Identities = 22/57 (38%), Positives = 30/57 (52%), Gaps = 1/57 (1%)
Frame = +2
Query: 320 NNIKLMYKRDGLALTLDDENSNDGRLAYG-DGKDKTSPKVSWKFVPLWENNKVYFKI 487
++IKL + L DD+ D LA+ G+D TS +SW F L EN +V KI
Sbjct: 281 SHIKLDTTKYELLNPSDDKFLRDTILAFNLAGRDTTSSALSWFFWLLSENPQVVTKI 337
>At3g27720.1 68416.m03461 zinc finger protein-related contains
Pfam:PF01485 IBR domain
Length = 493
Score = 29.9 bits (64), Expect = 1.8
Identities = 19/64 (29%), Positives = 34/64 (53%)
Frame = +3
Query: 54 YANETSVSDSKLEDDLYNSILVADYDHSVEKSKQIYEDKKSEVITNVVNKLIRNNKMNCM 233
Y + + KLED L SIL +S K ++++ K+ I + VN+L R+ ++ +
Sbjct: 313 YRYKAHIDSLKLEDKLKKSILKKAVLNSETKDQKVF--KEYSWIIDAVNRLFRSRRI--L 368
Query: 234 EYAY 245
Y+Y
Sbjct: 369 SYSY 372
>At2g17260.1 68415.m01993 glutamate receptor family protein (GLR3.1)
(GLR2) identical to putative glutamate receptor GLR2
[Arabidopsis thaliana] gi|4185740|gb|AAD09174; plant
glutamate receptor family, PMID:11379626
Length = 951
Score = 29.9 bits (64), Expect = 1.8
Identities = 15/35 (42%), Positives = 22/35 (62%)
Frame = +2
Query: 347 DGLALTLDDENSNDGRLAYGDGKDKTSPKVSWKFV 451
D +AL DD+NS +G A GD ++ K+S+K V
Sbjct: 194 DVVALYNDDDNSRNGVTALGDELEERRCKISYKAV 228
>At3g59690.1 68416.m06660 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 517
Score = 29.5 bits (63), Expect = 2.3
Identities = 16/69 (23%), Positives = 34/69 (49%)
Frame = +3
Query: 63 ETSVSDSKLEDDLYNSILVADYDHSVEKSKQIYEDKKSEVITNVVNKLIRNNKMNCMEYA 242
E + K + L +S + D+D SV + +++K + ++ +I+ + Y+
Sbjct: 236 ENRARNDKDDTKLVSSRMSDDWDDSV-----LTKEEKDVRLHRKIDAMIKRERSMAYAYS 290
Query: 243 YQLWSKAPK 269
+QLW +PK
Sbjct: 291 HQLWKNSPK 299
>At5g63450.1 68418.m07965 cytochrome P450, putative
Length = 510
Score = 28.3 bits (60), Expect = 5.4
Identities = 13/33 (39%), Positives = 21/33 (63%)
Frame = +2
Query: 410 GKDKTSPKVSWKFVPLWENNKVYFKIVNTQRNQ 508
G+D TS ++W F L +N+ V KI++ RN+
Sbjct: 306 GRDTTSAAMTWLFWLLSQNDDVETKILDELRNK 338
>At1g29510.1 68414.m03610 auxin-responsive protein, putative similar
to auxin-induced protein 6B (SP:P33083) [Glycine max]
Length = 143
Score = 28.3 bits (60), Expect = 5.4
Identities = 17/64 (26%), Positives = 32/64 (50%)
Frame = -3
Query: 529 FELPTSNLITLSVHNLEVDLVVLPQRNELPADFWTRLVLAIAVGKSAIVAVLIVQRQSET 350
F LPT ITL ++ ++ ++ + + D L+++I+ K ++ L+ Q QS
Sbjct: 79 FGLPTEGPITLPFDSVFLEYLIKLIQRRMDGDTEKALLMSISSAKCSLQCSLLQQEQSTQ 138
Query: 349 VALV 338
LV
Sbjct: 139 QLLV 142
>At4g35290.2 68417.m05015 glutamate receptor family protein (GLR3.2)
(GLUR2) identical to putative glutamate receptor
like-protein GLUR2 [Arabidopsis thaliana]
gi|13160471|gb|AAK13248; plant glutamate receptor
family, PMID:11379626
Length = 912
Score = 27.9 bits (59), Expect = 7.2
Identities = 14/33 (42%), Positives = 20/33 (60%)
Frame = +2
Query: 353 LALTLDDENSNDGRLAYGDGKDKTSPKVSWKFV 451
+AL DD+NS +G A GD + K+S+K V
Sbjct: 166 IALYNDDDNSRNGITALGDELEGRRCKISYKAV 198
>At4g35290.1 68417.m05014 glutamate receptor family protein (GLR3.2)
(GLUR2) identical to putative glutamate receptor
like-protein GLUR2 [Arabidopsis thaliana]
gi|13160471|gb|AAK13248; plant glutamate receptor
family, PMID:11379626
Length = 912
Score = 27.9 bits (59), Expect = 7.2
Identities = 14/33 (42%), Positives = 20/33 (60%)
Frame = +2
Query: 353 LALTLDDENSNDGRLAYGDGKDKTSPKVSWKFV 451
+AL DD+NS +G A GD + K+S+K V
Sbjct: 166 IALYNDDDNSRNGITALGDELEGRRCKISYKAV 198
>At4g35000.1 68417.m04963 L-ascorbate peroxidase 3 (APX3) identical
to ascorbate peroxidase 3 [Arabidopsis thaliana]
GI:2444019, L-ascorbate peroxidase [Arabidopsis
thaliana] gi|1523791|emb|CAA66926; similar to ascorbate
peroxidase [Gossypium hirsutum] gi|1019946|gb|AAB52954
Length = 287
Score = 27.9 bits (59), Expect = 7.2
Identities = 15/37 (40%), Positives = 19/37 (51%)
Frame = +1
Query: 577 GFKAQWTLQPAKYDNDVLFFMYNREYNEALVLSRPTD 687
GF WT +P K+DN + E +E L L PTD
Sbjct: 171 GFDGPWTQEPLKFDNSYFVELLKGE-SEGL-LKLPTD 205
>At4g13040.1 68417.m02035 AP2 domain-containing transcription factor
family protein contains Pfam profile PF00847: AP2 domain
Length = 226
Score = 27.9 bits (59), Expect = 7.2
Identities = 11/45 (24%), Positives = 23/45 (51%)
Frame = +1
Query: 76 PTPNSKTIFTTASSLPITTIPLKRANRSTRTRRAKSSQMS*TNSY 210
P PN++ + + +TT+ +++ R+RR +S M + Y
Sbjct: 36 PNPNARAAYNPGPAETVTTVIVEKKAIEERSRRTRSKHMHFRSDY 80
>At5g57180.2 68418.m07143 expressed protein ; supporting cDNA
gi|13991645|gb|AF359387.1|AF359387
Length = 435
Score = 27.5 bits (58), Expect = 9.5
Identities = 19/48 (39%), Positives = 27/48 (56%), Gaps = 6/48 (12%)
Frame = +1
Query: 64 KPQSPTPNSKTIFTTASSLPITTI------PLKRANRSTRTRRAKSSQ 189
K +SP P+S T T A+S P +TI PL + R RT+R + +Q
Sbjct: 21 KVKSPPPSSSTTTTRATS-PSSTISESSNSPLAISTRKPRTQRKRPNQ 67
>At5g57180.1 68418.m07142 expressed protein ; supporting cDNA
gi|13991645|gb|AF359387.1|AF359387
Length = 424
Score = 27.5 bits (58), Expect = 9.5
Identities = 19/48 (39%), Positives = 27/48 (56%), Gaps = 6/48 (12%)
Frame = +1
Query: 64 KPQSPTPNSKTIFTTASSLPITTI------PLKRANRSTRTRRAKSSQ 189
K +SP P+S T T A+S P +TI PL + R RT+R + +Q
Sbjct: 21 KVKSPPPSSSTTTTRATS-PSSTISESSNSPLAISTRKPRTQRKRPNQ 67
>At5g47730.1 68418.m05897 SEC14 cytosolic factor, putative /
polyphosphoinositide-binding protein, putative similar
to polyphosphoinositide binding protein SEC14 homolog
Ssh1p (GI:2739044) {Glycine max}
Length = 341
Score = 27.5 bits (58), Expect = 9.5
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = +2
Query: 317 ENNIKLMYKRDGLALTLDDENSNDGRLAYGD 409
E+ + R+GLA+++DD S+ LA GD
Sbjct: 310 ESELHKFENRNGLAVSIDDRKSSHETLANGD 340
>At4g00620.1 68417.m00086 tetrahydrofolate
dehydrogenase/cyclohydrolase, putative similar to
SP|P07245 C-1-tetrahydrofolate synthase, cytoplasmic
(C1-THF synthase) [Includes: Methylenetetrahydrofolate
dehydrogenase (EC 1.5.1.5); Methenyltetrahydrofolate
cyclohydrolase (EC 3.5.4.9); Formyltetrahydrofolate
synthetase (EC 6.3.4.3)] {Saccharomyces cerevisiae};
contains Pfam profiles PF02882: Tetrahydrofolate
dehydrogenase/cyclohydrolase, NAD(P)-binding domain,
PF00763: Tetrahydrofolate dehydrogenase/cyclohydrolase,
catalytic domain
Length = 360
Score = 27.5 bits (58), Expect = 9.5
Identities = 12/29 (41%), Positives = 21/29 (72%), Gaps = 3/29 (10%)
Frame = -3
Query: 412 AIAVGKSAIV---AVLIVQRQSETVALVH 335
A+ +G+S IV A L++QR+ TV+++H
Sbjct: 230 AVVIGRSNIVGMPAALLLQREDATVSIIH 258
>At3g13540.1 68416.m01702 myb family transcription factor contains
Pfam profile: PF00249 myb-like DNA-binding domain
Length = 249
Score = 27.5 bits (58), Expect = 9.5
Identities = 15/53 (28%), Positives = 29/53 (54%)
Frame = +3
Query: 42 VASLYANETSVSDSKLEDDLYNSILVADYDHSVEKSKQIYEDKKSEVITNVVN 200
++S + ++T+V+ + D NSI V +H E Y+DK S + +++N
Sbjct: 163 ISSSHTDDTTVNGG--DGDSKNSINVFGGEHGYEDFGFCYDDKFSSFLNSLIN 213
>At2g32390.1 68415.m03958 glutamate receptor family protein (GLR3.5)
plant glutamate receptor family, PMID:11379626
Length = 895
Score = 27.5 bits (58), Expect = 9.5
Identities = 15/46 (32%), Positives = 25/46 (54%), Gaps = 2/46 (4%)
Frame = +2
Query: 344 RDGLALTLDDENSNDGRLAYGDGKDKTSPKVSWK--FVPLWENNKV 475
R+ +A+ +DDE +G GD K K+S+K F P +N+ +
Sbjct: 123 REVVAIFVDDEYGRNGISVLGDALAKKRAKISYKAAFPPGADNSSI 168
>At1g02640.1 68414.m00214 glycosyl hydrolase family 3 protein
similar to beta-xylosidase GB:Z84377 GI:2102655 from
[Aspergillus niger]
Length = 768
Score = 27.5 bits (58), Expect = 9.5
Identities = 18/58 (31%), Positives = 27/58 (46%), Gaps = 4/58 (6%)
Frame = -2
Query: 719 LYPNAMRLPQVSVGLESTNASLYSRL--YMKNKTSLSYLAGC--RVHWALNPSTLLTP 558
+YP A PQV + S NASL+ + + N+ Y G +W+ N + L P
Sbjct: 108 VYPAATSFPQVITTVASFNASLWESIGRVVSNEARAMYNGGVGGLTYWSPNVNILRDP 165
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,563,799
Number of Sequences: 28952
Number of extensions: 256017
Number of successful extensions: 958
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 931
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 958
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1565336320
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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