BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00701 (411 letters) Database: rice 37,544 sequences; 14,793,348 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value 04_04_0729 + 27612106-27612195,27612284-27612370,27612469-276125... 95 3e-20 06_03_1193 - 28298656-28298754,28299303-28299377,28299456-282995... 87 7e-18 05_07_0067 + 27463703-27463705,27464309-27464365,27464779-274648... 83 1e-16 02_02_0253 - 8325437-8325628,8325705-8325779,8325933-8326037,832... 83 1e-16 06_03_0353 + 19810945-19810975,19811308-19811369,19811741-198118... 82 1e-16 06_03_0477 - 21250811-21250981,21251261-21251374,21251447-212516... 81 3e-16 03_05_0339 + 23287620-23287676,23287784-23287849,23287937-232880... 81 4e-16 02_02_0739 - 13560241-13560622,13561192-13561239,13563727-13564949 73 7e-14 03_06_0362 + 33379489-33379624,33380161-33380423,33380524-333806... 71 4e-13 10_08_0620 - 19321612-19323237 64 4e-11 05_01_0337 - 2658647-2658722,2658797-2658915,2658995-2659102,265... 64 5e-11 12_01_1011 + 10292709-10292765,10293003-10293156,10294062-102941... 42 1e-04 02_01_0024 + 159595-159801,159892-160017,160201-160338,160431-16... 42 1e-04 06_01_0097 - 810283-810296,810398-810521,810974-811066,811144-81... 41 3e-04 03_06_0760 - 36067752-36068045,36068260-36068711,36069263-360695... 41 3e-04 03_01_0305 - 2383187-2383298,2383606-2383684,2383762-2383867,238... 40 8e-04 05_06_0240 - 26629218-26629323,26629412-26629490,26629656-266297... 36 0.010 10_08_0289 + 16534063-16534100,16534219-16534243,16534847-165349... 33 0.12 01_06_1809 - 40029628-40030539 27 5.8 07_03_1649 - 28385289-28385358,28387039-28387877 27 7.7 03_03_0113 + 14550435-14550822,14550831-14551660,14552105-145522... 27 7.7 02_04_0387 - 22548778-22548917,22550635-22550716 27 7.7 >04_04_0729 + 27612106-27612195,27612284-27612370,27612469-27612528, 27612554-27612688,27612769-27612864,27613149-27613214, 27613647-27613864,27614093-27614189,27614605-27614691, 27614892-27615026,27615121-27615225,27615717-27615845, 27615925-27616028,27616598-27616688,27616774-27616857 Length = 527 Score = 94.7 bits (225), Expect = 3e-20 Identities = 44/48 (91%), Positives = 47/48 (97%) Frame = +1 Query: 109 MLVDDIGDVTVTNDGATILKMLEVEHPAAKVLVELAQLQDEEVGDGTT 252 MLVDDIGDVT+TNDGATIL+MLEVEHPAAKVLVELA+LQD EVGDGTT Sbjct: 1 MLVDDIGDVTITNDGATILRMLEVEHPAAKVLVELAELQDREVGDGTT 48 Score = 48.4 bits (110), Expect = 2e-06 Identities = 23/30 (76%), Positives = 26/30 (86%) Frame = +3 Query: 255 VVIIAAELLKNADELVKTKIHPTSIISGXR 344 VVIIAAELLK ++LV+ KIHPTSIISG R Sbjct: 50 VVIIAAELLKRGNDLVRNKIHPTSIISGYR 79 >06_03_1193 - 28298656-28298754,28299303-28299377,28299456-28299544, 28299679-28299760,28300385-28300474,28300557-28300655, 28300733-28300852,28300968-28301084,28301174-28301309, 28301415-28301584,28301744-28301947,28302032-28302157, 28302841-28303101,28303215-28303229 Length = 560 Score = 86.6 bits (205), Expect = 7e-18 Identities = 37/82 (45%), Positives = 58/82 (70%) Frame = +1 Query: 40 NVMXXXXXXNIVKSSLGPVGLDKMLVDDIGDVTVTNDGATILKMLEVEHPAAKVLVELAQ 219 N+ + V+++LGP G+DK++ DD G T++NDGATI+++L++ HPAAK+LV++A+ Sbjct: 28 NINACTVVADTVRTTLGPRGMDKLIHDDKGGTTISNDGATIMRLLDIIHPAAKILVDIAK 87 Query: 220 LQDEEVGDGTTXXXXXXXNYLR 285 QD EVGDGTT +L+ Sbjct: 88 SQDSEVGDGTTTVVLLAAEFLK 109 Score = 33.5 bits (73), Expect = 0.067 Identities = 17/45 (37%), Positives = 26/45 (57%) Frame = +3 Query: 255 VVIIAAELLKNADELVKTKIHPTSIISGXRXAC*EAVKYIPDNLT 389 VV++AAE LK A ++ +HP S+I R A A++ + D T Sbjct: 100 VVLLAAEFLKEAKPYIEDGVHPHSLIRSYRTAGHLAIEKVKDLAT 144 >05_07_0067 + 27463703-27463705,27464309-27464365,27464779-27464844, 27464985-27465096,27465281-27465409,27466076-27466191, 27466364-27466440,27466678-27467163,27467255-27467570, 27467618-27467797 Length = 513 Score = 82.6 bits (195), Expect = 1e-16 Identities = 44/96 (45%), Positives = 62/96 (64%), Gaps = 3/96 (3%) Frame = +1 Query: 7 TRSCGDPVRTQNVMXXXXXXNIVKSSLGPVGLDKMLVDDIG---DVTVTNDGATILKMLE 177 T G+ R + + ++VK++LGP G+DK+L G VTVTNDGATILK L Sbjct: 11 TEEKGERARMASFVGGMAISDLVKTTLGPKGMDKIL-QSTGRGRSVTVTNDGATILKSLH 69 Query: 178 VEHPAAKVLVELAQLQDEEVGDGTTXXXXXXXNYLR 285 +++PAAKVLV+++++QD+EVGDGTT LR Sbjct: 70 IDNPAAKVLVDISKVQDDEVGDGTTSVVVLAGELLR 105 Score = 35.9 bits (79), Expect = 0.013 Identities = 15/27 (55%), Positives = 22/27 (81%) Frame = +3 Query: 255 VVIIAAELLKNADELVKTKIHPTSIIS 335 VV++A ELL+ A++LV KIHP +II+ Sbjct: 96 VVVLAGELLREAEKLVNMKIHPMTIIA 122 >02_02_0253 - 8325437-8325628,8325705-8325779,8325933-8326037, 8326249-8326374,8326451-8326835,8326931-8327208, 8327294-8327367,8327476-8327547,8327637-8327705, 8340132-8340201,8340806-8340873,8341571-8341643, 8341807-8341868,8342168-8342198 Length = 559 Score = 82.6 bits (195), Expect = 1e-16 Identities = 38/81 (46%), Positives = 54/81 (66%) Frame = +1 Query: 10 RSCGDPVRTQNVMXXXXXXNIVKSSLGPVGLDKMLVDDIGDVTVTNDGATILKMLEVEHP 189 R G V N+ +I++++LGP + KML+D G + VTNDG IL+ L++ HP Sbjct: 16 RESGTKVHHANIQAAKAVADIIRTTLGPQSMLKMLLDAAGGIVVTNDGNCILRELDLAHP 75 Query: 190 AAKVLVELAQLQDEEVGDGTT 252 AAK ++EL++ QDEEVGDGTT Sbjct: 76 AAKSMIELSRTQDEEVGDGTT 96 >06_03_0353 + 19810945-19810975,19811308-19811369,19811741-19811813, 19813292-19813359,19813496-19813565,19813908-19813976, 19814065-19814136,19814247-19814320,19814420-19814697, 19814804-19815188,19815268-19815393,19815583-19815687, 19815986-19815994,19817040-19817114,19817202-19817390 Length = 561 Score = 82.2 bits (194), Expect = 1e-16 Identities = 37/81 (45%), Positives = 54/81 (66%) Frame = +1 Query: 10 RSCGDPVRTQNVMXXXXXXNIVKSSLGPVGLDKMLVDDIGDVTVTNDGATILKMLEVEHP 189 R G V N+ +I++++LGP + KML+D G + VTNDG IL+ +++ HP Sbjct: 16 RESGTKVHHANIQAAKAVADIIRTTLGPRSMLKMLLDASGGIVVTNDGNAILREIDIAHP 75 Query: 190 AAKVLVELAQLQDEEVGDGTT 252 AAK ++EL++ QDEEVGDGTT Sbjct: 76 AAKSMIELSRTQDEEVGDGTT 96 >06_03_0477 - 21250811-21250981,21251261-21251374,21251447-21251613, 21252051-21252153,21252315-21252367,21252438-21252570, 21252835-21253183,21253685-21254003,21254519-21254717 Length = 535 Score = 81.4 bits (192), Expect = 3e-16 Identities = 39/82 (47%), Positives = 56/82 (68%) Frame = +1 Query: 7 TRSCGDPVRTQNVMXXXXXXNIVKSSLGPVGLDKMLVDDIGDVTVTNDGATILKMLEVEH 186 +R G + N+ I+++SLGP G+DKML GDVT+TNDGATIL+ ++V++ Sbjct: 22 SRLRGLDAQKANIAAGKAVARILRTSLGPKGMDKMLQSPDGDVTITNDGATILEQMDVDN 81 Query: 187 PAAKVLVELAQLQDEEVGDGTT 252 AK++VEL+ QD E+GDGTT Sbjct: 82 QIAKLMVELSCSQDYEIGDGTT 103 >03_05_0339 + 23287620-23287676,23287784-23287849,23287937-23288048, 23288240-23288368,23288795-23288866,23289245-23289360, 23289494-23289570,23289788-23290273,23290373-23290688, 23290769-23290915 Length = 525 Score = 80.6 bits (190), Expect = 4e-16 Identities = 43/92 (46%), Positives = 60/92 (65%), Gaps = 3/92 (3%) Frame = +1 Query: 19 GDPVRTQNVMXXXXXXNIVKSSLGPVGLDKMLVDDIG---DVTVTNDGATILKMLEVEHP 189 G+ R + ++VK++LGP G+DK+L G VTVTNDGATILK L +++P Sbjct: 14 GERARMAAFIGAMAIADLVKTTLGPKGMDKIL-QSTGRGRSVTVTNDGATILKSLHIDNP 72 Query: 190 AAKVLVELAQLQDEEVGDGTTXXXXXXXNYLR 285 AAKVLV+++++QD+EVGDGTT LR Sbjct: 73 AAKVLVDISKVQDDEVGDGTTSVVVLAGELLR 104 Score = 41.1 bits (92), Expect = 3e-04 Identities = 18/32 (56%), Positives = 25/32 (78%) Frame = +3 Query: 255 VVIIAAELLKNADELVKTKIHPTSIISGXRXA 350 VV++A ELL+ A++LV KIHP +II+G R A Sbjct: 95 VVVLAGELLREAEKLVNMKIHPMTIIAGYRMA 126 >02_02_0739 - 13560241-13560622,13561192-13561239,13563727-13564949 Length = 550 Score = 73.3 bits (172), Expect = 7e-14 Identities = 37/88 (42%), Positives = 52/88 (59%) Frame = +1 Query: 22 DPVRTQNVMXXXXXXNIVKSSLGPVGLDKMLVDDIGDVTVTNDGATILKMLEVEHPAAKV 201 D VR N+ + V++SLGP G+DKM+ +V +TNDGATIL + + PAA++ Sbjct: 25 DDVRGLNIAAARAVADAVRTSLGPRGMDKMISSGEQEVIITNDGATILSRMSLLQPAARM 84 Query: 202 LVELAQLQDEEVGDGTTXXXXXXXNYLR 285 L EL++ QD GDGTT + LR Sbjct: 85 LAELSRSQDAAAGDGTTTVVVLAGSLLR 112 >03_06_0362 + 33379489-33379624,33380161-33380423,33380524-33380670, 33380753-33380914,33381681-33381758,33382329-33382462, 33382702-33382774,33382852-33382929,33383002-33383124, 33383223-33383317,33383723-33383932,33384115-33384172, 33384268-33384333 Length = 540 Score = 70.9 bits (166), Expect = 4e-13 Identities = 31/59 (52%), Positives = 45/59 (76%) Frame = +1 Query: 70 IVKSSLGPVGLDKMLVDDIGDVTVTNDGATILKMLEVEHPAAKVLVELAQLQDEEVGDG 246 I ++SLGP G++KM+++ + + VTND ATI+ LEV+HPAAK+LV + Q EE+GDG Sbjct: 37 ITRTSLGPNGMNKMVINHLDKLFVTNDAATIVNELEVQHPAAKILVLAGRAQQEEIGDG 95 Score = 35.1 bits (77), Expect = 0.022 Identities = 15/44 (34%), Positives = 27/44 (61%) Frame = +3 Query: 249 HIVVIIAAELLKNADELVKTKIHPTSIISGXRXAC*EAVKYIPD 380 ++ + A ELL+ A+EL++ +HP+ II G A + V+ + D Sbjct: 97 NLTISFAGELLEKAEELIRMGLHPSEIIIGYTKAINKTVEILED 140 >10_08_0620 - 19321612-19323237 Length = 541 Score = 64.1 bits (149), Expect = 4e-11 Identities = 37/92 (40%), Positives = 51/92 (55%), Gaps = 4/92 (4%) Frame = +1 Query: 22 DPVRTQNVMXXXXXXNIVKSSLGPVGLDKMLVDDI--GD--VTVTNDGATILKMLEVEHP 189 D VR N+ ++SLGP G+DKM+ GD V +TNDGATIL + + P Sbjct: 25 DDVRGLNIAAGRAVAAAARTSLGPRGMDKMISSSSSGGDQAVIITNDGATILSRMPLLQP 84 Query: 190 AAKVLVELAQLQDEEVGDGTTXXXXXXXNYLR 285 AA++L +L++ QD GDGTT + LR Sbjct: 85 AARMLADLSRSQDAAAGDGTTTVVVLAGSLLR 116 >05_01_0337 - 2658647-2658722,2658797-2658915,2658995-2659102, 2659187-2659255,2659561-2659655,2659740-2659944, 2660125-2660211,2660291-2660405,2660696-2660802, 2661054-2661146,2661243-2661290,2661345-2661544, 2661993-2662128,2662228-2662291,2662779-2662912 Length = 551 Score = 63.7 bits (148), Expect = 5e-11 Identities = 28/71 (39%), Positives = 45/71 (63%) Frame = +1 Query: 40 NVMXXXXXXNIVKSSLGPVGLDKMLVDDIGDVTVTNDGATILKMLEVEHPAAKVLVELAQ 219 N+ +++K++LGP G KMLV GD+ +T DG T+LK +++++P A ++ A Sbjct: 22 NINAAKGLQDVLKTNLGPKGTIKMLVGGAGDIKLTKDGNTLLKEMQIQNPTAIMIARTAV 81 Query: 220 LQDEEVGDGTT 252 QD+ GDGTT Sbjct: 82 AQDDTSGDGTT 92 >12_01_1011 + 10292709-10292765,10293003-10293156,10294062-10294183, 10294288-10294495,10294644-10294789,10294920-10295226, 10295666-10296117,10296388-10296678 Length = 578 Score = 42.3 bits (95), Expect = 1e-04 Identities = 25/66 (37%), Positives = 38/66 (57%), Gaps = 4/66 (6%) Frame = +1 Query: 67 NIVKSSLGPVGLDKMLVDDIGDVTVTNDGATILKMLEV----EHPAAKVLVELAQLQDEE 234 N V +LGP G + +L D+ G V NDG TI + +E+ E+ A ++ E+A ++ Sbjct: 61 NAVGVTLGPRGRNVVL-DEYGSPKVVNDGVTIARAIELYDPMENAGAALIREVASKTNDS 119 Query: 235 VGDGTT 252 GDGTT Sbjct: 120 AGDGTT 125 >02_01_0024 + 159595-159801,159892-160017,160201-160338,160431-160526, 160958-161200,161249-161537,161612-161781,161975-162052, 162156-162227,162345-162449,162555-162647,162737-162829, 163109-163235,163317-163330 Length = 616 Score = 42.3 bits (95), Expect = 1e-04 Identities = 23/66 (34%), Positives = 37/66 (56%), Gaps = 4/66 (6%) Frame = +1 Query: 67 NIVKSSLGPVGLDKMLVDDIGDVTVTNDGATILKMLEVEHP----AAKVLVELAQLQDEE 234 ++V +LGP G + +L G + NDG T+ K +E+E P AK++ + A ++ Sbjct: 77 DLVGVTLGPKGRNVVLESKYGSPKIVNDGVTVAKEVELEDPVENIGAKLVRQAAAKTNDL 136 Query: 235 VGDGTT 252 GDGTT Sbjct: 137 AGDGTT 142 >06_01_0097 - 810283-810296,810398-810521,810974-811066,811144-811236, 811410-811514,811661-811732,811857-811934,812210-812379, 812507-812741,812832-813074,813243-813338,813445-813582, 814014-814139,814235-814453 Length = 601 Score = 41.1 bits (92), Expect = 3e-04 Identities = 22/66 (33%), Positives = 37/66 (56%), Gaps = 4/66 (6%) Frame = +1 Query: 67 NIVKSSLGPVGLDKMLVDDIGDVTVTNDGATILKMLEVEHP----AAKVLVELAQLQDEE 234 ++V +LGP G + +L G + NDG T+ + +E+E P AK++ + A ++ Sbjct: 81 DLVGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREVELEDPVENIGAKLVRQAAAKTNDL 140 Query: 235 VGDGTT 252 GDGTT Sbjct: 141 AGDGTT 146 >03_06_0760 - 36067752-36068045,36068260-36068711,36069263-36069569, 36069719-36069864,36069989-36070196,36070339-36070460, 36070621-36070789,36070875-36070931 Length = 584 Score = 41.1 bits (92), Expect = 3e-04 Identities = 24/64 (37%), Positives = 36/64 (56%), Gaps = 4/64 (6%) Frame = +1 Query: 73 VKSSLGPVGLDKMLVDDIGDVTVTNDGATILKMLEVEHP----AAKVLVELAQLQDEEVG 240 V +LGP G + +L D+ G V NDG TI + +E+ P A ++ E+A ++ G Sbjct: 68 VAVTLGPRGRNVVL-DEFGSPKVVNDGVTIARAIELADPMENAGAALIREVASKTNDSAG 126 Query: 241 DGTT 252 DGTT Sbjct: 127 DGTT 130 >03_01_0305 - 2383187-2383298,2383606-2383684,2383762-2383867, 2383941-2384044,2384141-2384240,2384533-2384628, 2384724-2384801,2385355-2385423,2385511-2385591, 2385670-2385759,2385859-2385945,2386022-2386126, 2386591-2386686,2386796-2386971,2387225-2387384, 2387898-2388029,2388236-2388260,2389023-2389174 Length = 615 Score = 39.9 bits (89), Expect = 8e-04 Identities = 21/64 (32%), Positives = 35/64 (54%), Gaps = 4/64 (6%) Frame = +1 Query: 73 VKSSLGPVGLDKMLVDDIGDVTVTNDGATILKMLE----VEHPAAKVLVELAQLQDEEVG 240 VK ++GP G ++ G VT DG T+ K +E V++ A ++ ++A ++ G Sbjct: 96 VKVTMGPKGRTVVIEQSFGAPKVTKDGVTVAKSIEFSNRVKNVGASLVKQVANATNDTAG 155 Query: 241 DGTT 252 DGTT Sbjct: 156 DGTT 159 >05_06_0240 - 26629218-26629323,26629412-26629490,26629656-26629761, 26629844-26629947,26630069-26630168,26630256-26630351, 26630464-26630541,26630603-26630671,26630766-26630846, 26630879-26630935,26631016-26631105,26631192-26631278, 26631690-26631794,26631899-26631994,26632222-26632397, 26632847-26633006,26633151-26633600 Length = 679 Score = 36.3 bits (80), Expect = 0.010 Identities = 21/64 (32%), Positives = 35/64 (54%), Gaps = 4/64 (6%) Frame = +1 Query: 73 VKSSLGPVGLDKMLVDDIGDVTVTNDGATILKMLEVEHPA----AKVLVELAQLQDEEVG 240 VK ++GP G + ++ VT DG T+ K +E E A A ++ ++A+ ++ G Sbjct: 143 VKVTMGPKGRNVIIERSHRAPKVTKDGVTVAKSIEFEDSAKNVGANLVKQVAEATNKVAG 202 Query: 241 DGTT 252 DGTT Sbjct: 203 DGTT 206 >10_08_0289 + 16534063-16534100,16534219-16534243,16534847-16534978, 16535255-16535441,16536014-16536189,16536282-16536377, 16536511-16536615,16536654-16536683,16536698-16536784, 16536874-16536963,16537033-16537113,16537200-16537268, 16537413-16537490,16537578-16537673,16537981-16538080, 16538208-16538311,16538396-16538501,16538595-16538673, 16538861-16538963 Length = 593 Score = 32.7 bits (71), Expect = 0.12 Identities = 17/56 (30%), Positives = 30/56 (53%), Gaps = 4/56 (7%) Frame = +1 Query: 97 GLDKMLVDDIGDVTVTNDGATILKMLE----VEHPAAKVLVELAQLQDEEVGDGTT 252 G + ++ G VT DG T+ K +E V++ A ++ ++A ++ GDGTT Sbjct: 75 GRNVVIEQSYGSPKVTKDGVTVAKSIEFKDRVKNVGASLVKQVANATNDTAGDGTT 130 >01_06_1809 - 40029628-40030539 Length = 303 Score = 27.1 bits (57), Expect = 5.8 Identities = 14/30 (46%), Positives = 16/30 (53%), Gaps = 2/30 (6%) Frame = -1 Query: 87 KGALYYVCYSRCCHYVLCSH--WVSAGSCP 4 KGA + C C LCS WVS G+CP Sbjct: 263 KGAAFIPCGHTFCR--LCSRELWVSRGNCP 290 >07_03_1649 - 28385289-28385358,28387039-28387877 Length = 302 Score = 26.6 bits (56), Expect = 7.7 Identities = 14/47 (29%), Positives = 25/47 (53%) Frame = +1 Query: 112 LVDDIGDVTVTNDGATILKMLEVEHPAAKVLVELAQLQDEEVGDGTT 252 L+ D GDV+ ++ +++ L+ + P + VLV D + GTT Sbjct: 244 LLVDSGDVSAGSEADGVMRWLDGQEPGSVVLVSFG--SDSKTKKGTT 288 >03_03_0113 + 14550435-14550822,14550831-14551660,14552105-14552278, 14552350-14552517 Length = 519 Score = 26.6 bits (56), Expect = 7.7 Identities = 12/29 (41%), Positives = 19/29 (65%) Frame = +3 Query: 267 AAELLKNADELVKTKIHPTSIISGXRXAC 353 AAEL + E + +++ P SI++G R AC Sbjct: 6 AAELPDDIVEKIISRLPPRSIVTGCRAAC 34 >02_04_0387 - 22548778-22548917,22550635-22550716 Length = 73 Score = 26.6 bits (56), Expect = 7.7 Identities = 9/18 (50%), Positives = 10/18 (55%) Frame = -1 Query: 54 CCHYVLCSHWVSAGSCPC 1 CCHY C + S S PC Sbjct: 45 CCHYDKCYEYCSGDSGPC 62 Database: rice Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 14,793,348 Number of sequences in database: 37,544 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,183,890 Number of Sequences: 37544 Number of extensions: 175140 Number of successful extensions: 396 Number of sequences better than 10.0: 22 Number of HSP's better than 10.0 without gapping: 380 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 390 length of database: 14,793,348 effective HSP length: 75 effective length of database: 11,977,548 effective search space used: 730630428 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
- SilkBase 1999-2023 -