Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00695
(749 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g59990.2 68416.m06698 methionyl aminopeptidase, putative / me... 128 5e-30
At3g59990.1 68416.m06697 methionyl aminopeptidase, putative / me... 128 5e-30
At2g44180.1 68415.m05496 methionyl aminopeptidase, putative / me... 123 1e-28
At3g51800.2 68416.m05681 metallopeptidase M24 family protein sim... 51 9e-07
At3g51800.1 68416.m05680 metallopeptidase M24 family protein sim... 51 9e-07
At1g10130.1 68414.m01142 calcium-transporting ATPase 3, endoplas... 30 1.9
At4g13250.1 68417.m02060 short-chain dehydrogenase/reductase (SD... 29 2.5
At5g50630.1 68418.m06272 nodulin family protein similar to nodul... 29 3.3
At5g50520.1 68418.m06257 nodulin family protein similar to nodul... 29 3.3
At3g56840.1 68416.m06321 FAD-dependent oxidoreductase family pro... 29 3.3
At4g37040.1 68417.m05246 metallopeptidase M24 family protein sim... 29 4.4
At1g31510.1 68414.m03863 F-box family protein-related contains w... 29 4.4
At5g53550.1 68418.m06654 transporter, putative similar to iron-p... 28 5.8
At4g36760.2 68417.m05215 aminopeptidase P similar to Xaa-Pro ami... 28 5.8
At4g36760.1 68417.m05216 aminopeptidase P similar to Xaa-Pro ami... 28 5.8
At4g22970.1 68417.m03315 peptidase C50 family protein contains P... 28 7.6
At4g21240.1 68417.m03071 F-box family protein contains F-box dom... 28 7.6
>At3g59990.2 68416.m06698 methionyl aminopeptidase, putative /
methionine aminopeptidase, putative / peptidase M,
putative similar to Methionine aminopeptidase 2 (EC
3.4.11.18) from {Rattus norvegicus} SP|P38062, {Homo
sapiens} SP|P50579; contains Pfam profile PF00557:
metallopeptidase family M24; supporting cDNA
gi|11344921|gb|AF300880.1|AF300880
Length = 439
Score = 128 bits (308), Expect = 5e-30
Identities = 55/80 (68%), Positives = 68/80 (85%)
Frame = +3
Query: 510 EDGLKAGLAFPTGCSRNHCAAHYTPNTGDTTVLEYDDVVKIDFGTHINGRIIDCAFTLHF 689
E+GL+AG+AFPTGCS N AAH+TPN+GD TVL+YDDV+K+DFGTHI+G IIDCAFT+ F
Sbjct: 171 ENGLQAGIAFPTGCSLNWVAAHWTPNSGDKTVLQYDDVMKLDFGTHIDGHIIDCAFTVAF 230
Query: 690 NPRYDPLVKGVQEATEAGIK 749
NP +DPL+ +EAT GIK
Sbjct: 231 NPMFDPLLAASREATYTGIK 250
Score = 88.6 bits (210), Expect = 4e-18
Identities = 45/88 (51%), Positives = 57/88 (64%)
Frame = +1
Query: 256 GNFPEGQIMDHGPAEGIDERTAKNRFTSEEKRALDRLHKDIYQEIRHAAEAHRQTRKHIR 435
G FPEG+I ++ D+ R TSEEKR L+R K IY +R AAE HRQ RK++R
Sbjct: 93 GEFPEGEIQEYK-----DDNLW--RTTSEEKRELERFEKPIYNSVRRAAEVHRQVRKYVR 145
Query: 436 NWIKPGMTMIDICEELEKTARRLIGKMG 519
+ +KPGM M DICE LE T R+LI + G
Sbjct: 146 SIVKPGMLMTDICETLENTVRKLISENG 173
Score = 28.3 bits (60), Expect = 5.8
Identities = 12/18 (66%), Positives = 13/18 (72%)
Frame = +2
Query: 212 QTVPPTIPVAELFPVETF 265
QT PP+IPV ELFP F
Sbjct: 78 QTDPPSIPVVELFPSGEF 95
>At3g59990.1 68416.m06697 methionyl aminopeptidase, putative /
methionine aminopeptidase, putative / peptidase M,
putative similar to Methionine aminopeptidase 2 (EC
3.4.11.18) from {Rattus norvegicus} SP|P38062, {Homo
sapiens} SP|P50579; contains Pfam profile PF00557:
metallopeptidase family M24; supporting cDNA
gi|11344921|gb|AF300880.1|AF300880
Length = 439
Score = 128 bits (308), Expect = 5e-30
Identities = 55/80 (68%), Positives = 68/80 (85%)
Frame = +3
Query: 510 EDGLKAGLAFPTGCSRNHCAAHYTPNTGDTTVLEYDDVVKIDFGTHINGRIIDCAFTLHF 689
E+GL+AG+AFPTGCS N AAH+TPN+GD TVL+YDDV+K+DFGTHI+G IIDCAFT+ F
Sbjct: 171 ENGLQAGIAFPTGCSLNWVAAHWTPNSGDKTVLQYDDVMKLDFGTHIDGHIIDCAFTVAF 230
Query: 690 NPRYDPLVKGVQEATEAGIK 749
NP +DPL+ +EAT GIK
Sbjct: 231 NPMFDPLLAASREATYTGIK 250
Score = 88.6 bits (210), Expect = 4e-18
Identities = 45/88 (51%), Positives = 57/88 (64%)
Frame = +1
Query: 256 GNFPEGQIMDHGPAEGIDERTAKNRFTSEEKRALDRLHKDIYQEIRHAAEAHRQTRKHIR 435
G FPEG+I ++ D+ R TSEEKR L+R K IY +R AAE HRQ RK++R
Sbjct: 93 GEFPEGEIQEYK-----DDNLW--RTTSEEKRELERFEKPIYNSVRRAAEVHRQVRKYVR 145
Query: 436 NWIKPGMTMIDICEELEKTARRLIGKMG 519
+ +KPGM M DICE LE T R+LI + G
Sbjct: 146 SIVKPGMLMTDICETLENTVRKLISENG 173
Score = 28.3 bits (60), Expect = 5.8
Identities = 12/18 (66%), Positives = 13/18 (72%)
Frame = +2
Query: 212 QTVPPTIPVAELFPVETF 265
QT PP+IPV ELFP F
Sbjct: 78 QTDPPSIPVVELFPSGEF 95
>At2g44180.1 68415.m05496 methionyl aminopeptidase, putative /
methionine aminopeptidase, putative / peptidase M,
putative similar to SP|P50579 Methionine aminopeptidase
2 (EC 3.4.11.18) (MetAP 2) {Homo sapiens}; contains Pfam
profile PF00557: metallopeptidase family M24
Length = 441
Score = 123 bits (297), Expect = 1e-28
Identities = 52/80 (65%), Positives = 68/80 (85%)
Frame = +3
Query: 510 EDGLKAGLAFPTGCSRNHCAAHYTPNTGDTTVLEYDDVVKIDFGTHINGRIIDCAFTLHF 689
E+GL+AG+AFPTGCS N+ AAH+TPN+GD TVL+YDDV+K+DFGTHI+G I+D AFT+ F
Sbjct: 173 ENGLQAGIAFPTGCSLNNVAAHWTPNSGDKTVLQYDDVMKLDFGTHIDGHIVDSAFTVAF 232
Query: 690 NPRYDPLVKGVQEATEAGIK 749
NP +DPL+ ++AT GIK
Sbjct: 233 NPMFDPLLAASRDATYTGIK 252
Score = 88.2 bits (209), Expect = 5e-18
Identities = 44/88 (50%), Positives = 59/88 (67%)
Frame = +1
Query: 256 GNFPEGQIMDHGPAEGIDERTAKNRFTSEEKRALDRLHKDIYQEIRHAAEAHRQTRKHIR 435
G+FP+G+I + D+ R TSEEKR ++RL K IY +R AAE HRQ RK++R
Sbjct: 95 GDFPQGEIQQYN-----DDNLW--RTTSEEKREMERLQKPIYNSLRQAAEVHRQVRKYMR 147
Query: 436 NWIKPGMTMIDICEELEKTARRLIGKMG 519
+ +KPGM MID+CE LE T R+LI + G
Sbjct: 148 SILKPGMLMIDLCETLENTVRKLISENG 175
Score = 27.9 bits (59), Expect = 7.6
Identities = 12/18 (66%), Positives = 13/18 (72%)
Frame = +2
Query: 212 QTVPPTIPVAELFPVETF 265
QT PP+IPV ELFP F
Sbjct: 80 QTDPPSIPVLELFPSGDF 97
>At3g51800.2 68416.m05681 metallopeptidase M24 family protein
similar to SP|P50580 Proliferation-associated protein
2G4 {Mus musculus}; contains Pfam profile PF00557:
metallopeptidase family M24
Length = 401
Score = 50.8 bits (116), Expect = 9e-07
Identities = 23/48 (47%), Positives = 32/48 (66%)
Frame = +3
Query: 519 LKAGLAFPTGCSRNHCAAHYTPNTGDTTVLEYDDVVKIDFGTHINGRI 662
++ G+AFPT S N+ H++P D +VLE D+VKID G HI+G I
Sbjct: 73 IERGVAFPTCISVNNTVGHFSPLASDESVLEDGDMVKIDMGCHIDGFI 120
>At3g51800.1 68416.m05680 metallopeptidase M24 family protein
similar to SP|P50580 Proliferation-associated protein
2G4 {Mus musculus}; contains Pfam profile PF00557:
metallopeptidase family M24
Length = 392
Score = 50.8 bits (116), Expect = 9e-07
Identities = 23/48 (47%), Positives = 32/48 (66%)
Frame = +3
Query: 519 LKAGLAFPTGCSRNHCAAHYTPNTGDTTVLEYDDVVKIDFGTHINGRI 662
++ G+AFPT S N+ H++P D +VLE D+VKID G HI+G I
Sbjct: 73 IERGVAFPTCISVNNTVGHFSPLASDESVLEDGDMVKIDMGCHIDGFI 120
>At1g10130.1 68414.m01142 calcium-transporting ATPase 3, endoplasmic
reticulum-type (ACA6) (ECA3) nearly identical to
SP|Q9SY55 Calcium-transporting ATPase 3, endoplasmic
reticulum-type (EC 3.6.3.8) {Arabidopsis thaliana);
contains InterPro Accession IPR006069: Cation
transporting ATPase
Length = 998
Score = 29.9 bits (64), Expect = 1.9
Identities = 18/51 (35%), Positives = 26/51 (50%)
Frame = -3
Query: 435 ADVFSCLPVGFSSMSDLLINIFM*SIKSSFLLTCKTIFCSSLINTLSRTMV 283
A V +CL +G M+ L N + S+ S L C T+ CS TL+ M+
Sbjct: 309 AVVTTCLALGTKKMARL--NAIVRSLPSVETLGCTTVICSDKTGTLTTNMM 357
>At4g13250.1 68417.m02060 short-chain dehydrogenase/reductase (SDR)
family protein contains INTERPRO family IPR002198
Short-chain dehydrogenase/reductase (SDR) superfamily
Length = 496
Score = 29.5 bits (63), Expect = 2.5
Identities = 17/72 (23%), Positives = 37/72 (51%), Gaps = 3/72 (4%)
Frame = +1
Query: 334 TSEEKRALDRLHKDIYQEIRHA-AEAHRQTRKHIRNWIKPGMTMIDIC--EELEKTARRL 504
TS ++D K++ Q ++ + A RK + + G+ D+C E++EK +
Sbjct: 191 TSRSSESVDMTVKELEQNLKEIMSNASESARKKLSDAKVVGIAC-DVCKPEDVEKLSNFA 249
Query: 505 IGKMGSKQVWLS 540
+ ++GS +W++
Sbjct: 250 VKELGSINIWIN 261
>At5g50630.1 68418.m06272 nodulin family protein similar to
nodulin-like protein [Arabidopsis thaliana] GI:3329368,
nodule-specific protein Nlj70 [Lotus japonicus]
GI:3329366
Length = 540
Score = 29.1 bits (62), Expect = 3.3
Identities = 16/37 (43%), Positives = 21/37 (56%), Gaps = 1/37 (2%)
Frame = +1
Query: 199 IF*GADRPTYYTSSGTISC-GNFPEGQIMDHGPAEGI 306
IF G + TYY ++ +SC NFPE + GP GI
Sbjct: 119 IFVGTNGETYYNTASLVSCIHNFPESR----GPVVGI 151
>At5g50520.1 68418.m06257 nodulin family protein similar to
nodulin-like protein [Arabidopsis thaliana] GI:3329368,
nodule-specific protein Nlj70 [Lotus japonicus]
GI:3329366
Length = 540
Score = 29.1 bits (62), Expect = 3.3
Identities = 16/37 (43%), Positives = 21/37 (56%), Gaps = 1/37 (2%)
Frame = +1
Query: 199 IF*GADRPTYYTSSGTISC-GNFPEGQIMDHGPAEGI 306
IF G + TYY ++ +SC NFPE + GP GI
Sbjct: 119 IFVGTNGETYYNTASLVSCIHNFPESR----GPVVGI 151
>At3g56840.1 68416.m06321 FAD-dependent oxidoreductase family
protein contains Pfam profile PF01266 FAD dependent
oxidoreductase
Length = 483
Score = 29.1 bits (62), Expect = 3.3
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = -3
Query: 609 RELLYHLC*EYNVPHSGCGCIL 544
RELLY C EY +PH G ++
Sbjct: 146 RELLYKYCSEYEIPHKKIGKLI 167
>At4g37040.1 68417.m05246 metallopeptidase M24 family protein
similar to SP|O33343 Methionine aminopeptidase (EC
3.4.11.18) (Peptidase M) {Mycobacterium tuberculosis};
contains Pfam profile PF00557: metallopeptidase family
M24
Length = 350
Score = 28.7 bits (61), Expect = 4.4
Identities = 20/65 (30%), Positives = 29/65 (44%)
Frame = +3
Query: 552 SRNHCAAHYTPNTGDTTVLEYDDVVKIDFGTHINGRIIDCAFTLHFNPRYDPLVKGVQEA 731
S N C H P D+ LE D++ ID ++NG D + T F D K + E
Sbjct: 173 SVNECICHGIP---DSRPLEDGDIINIDVTVYLNGYHGDTSATF-FCGNVDEKAKKLVEV 228
Query: 732 TEAGI 746
T+ +
Sbjct: 229 TKESL 233
>At1g31510.1 68414.m03863 F-box family protein-related contains weak
hit to TIGRFAM TIGR01640 : F-box protein interaction
domain; ; similar to F-box protein family, AtFBX9
(GI:20197985) [Arabidopsis thaliana]
Length = 309
Score = 28.7 bits (61), Expect = 4.4
Identities = 16/66 (24%), Positives = 29/66 (43%)
Frame = -3
Query: 480 LFTNIYHCHARLDPIADVFSCLPVGFSSMSDLLINIFM*SIKSSFLLTCKTIFCSSLINT 301
+FT + A + P+ SC+ + F++ L +F + SS LL C L+
Sbjct: 10 IFTVFNYVPATVSPLCSTISCIGMDFNNKPSLNFQVFK-PLSSSDLLEHNMFHCDGLLLM 68
Query: 300 LSRTMV 283
+ T +
Sbjct: 69 VMGTKI 74
>At5g53550.1 68418.m06654 transporter, putative similar to
iron-phytosiderophore transporter protein yellow stripe
1 [Zea mays] GI:10770865; contains Pfam profile PF03169:
OPT oligopeptide transporter protein
Length = 669
Score = 28.3 bits (60), Expect = 5.8
Identities = 12/22 (54%), Positives = 15/22 (68%)
Frame = +1
Query: 208 GADRPTYYTSSGTISCGNFPEG 273
G +R TY S GT + GN+PEG
Sbjct: 133 GLNRITYEQSGGTHTDGNYPEG 154
>At4g36760.2 68417.m05215 aminopeptidase P similar to Xaa-Pro
aminopeptidase 2 [Lycopersicon esculentum] GI:15384991;
contains Pfam profile PF00557: metallopeptidase family
M24
Length = 519
Score = 28.3 bits (60), Expect = 5.8
Identities = 17/56 (30%), Positives = 23/56 (41%), Gaps = 2/56 (3%)
Frame = +3
Query: 528 GLAFPTGCS--RNHCAAHYTPNTGDTTVLEYDDVVKIDFGTHINGRIIDCAFTLHF 689
GL+FPT S N HY+P ++ D + D G D T+HF
Sbjct: 392 GLSFPTISSVGSNAAVIHYSPEPEACAEMDPDKIYLCDSGAQYLDGTTDITRTVHF 447
>At4g36760.1 68417.m05216 aminopeptidase P similar to Xaa-Pro
aminopeptidase 2 [Lycopersicon esculentum] GI:15384991;
contains Pfam profile PF00557: metallopeptidase family
M24
Length = 634
Score = 28.3 bits (60), Expect = 5.8
Identities = 17/56 (30%), Positives = 23/56 (41%), Gaps = 2/56 (3%)
Frame = +3
Query: 528 GLAFPTGCS--RNHCAAHYTPNTGDTTVLEYDDVVKIDFGTHINGRIIDCAFTLHF 689
GL+FPT S N HY+P ++ D + D G D T+HF
Sbjct: 392 GLSFPTISSVGSNAAVIHYSPEPEACAEMDPDKIYLCDSGAQYLDGTTDITRTVHF 447
>At4g22970.1 68417.m03315 peptidase C50 family protein contains Pfam
PF03568: Peptidase family C50
Length = 1773
Score = 27.9 bits (59), Expect = 7.6
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = -3
Query: 630 SSLHHHIRELLYHLC*EYNVPHSGCGCILW 541
+ LHHHI +L++ L NV C +LW
Sbjct: 662 TELHHHIYQLIFRLFKWKNVKLEVCLAMLW 691
>At4g21240.1 68417.m03071 F-box family protein contains F-box domain
Pfam:PF00646
Length = 417
Score = 27.9 bits (59), Expect = 7.6
Identities = 14/48 (29%), Positives = 26/48 (54%), Gaps = 1/48 (2%)
Frame = +3
Query: 564 CAAHYTPNTGDTTVLEYDDVVKIDFGTHINGRIIDC-AFTLHFNPRYD 704
C +Y P +G +EY+ + +F IN I++C FT+ + P ++
Sbjct: 358 CVLYYDPKSGSERRIEYEGIGDREF--CINTGILECKRFTIDWFPNHN 403
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,641,446
Number of Sequences: 28952
Number of extensions: 272929
Number of successful extensions: 766
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 744
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 766
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1663169840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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