BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00679 (714 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAP7G5.05 |rpl1002|rpl10-2, rpl10|60S ribosomal protein L10|Sch... 142 6e-35 SPBC18E5.04 |rpl1001|rpl10-1, rpl10|60S ribosomal protein L10|Sc... 142 6e-35 SPBC1348.02 |||S. pombe specific 5Tm protein family|Schizosaccha... 29 0.50 SPBPB2B2.19c |||S. pombe specific 5Tm protein family|Schizosacch... 29 0.50 SPAC977.01 |||S. pombe specific 5Tm protein family|Schizosacchar... 29 0.50 SPAC750.05c |||S. pombe specific 5Tm protein family|Schizosaccha... 29 0.50 SPAC19D5.03 |cid1||poly|Schizosaccharomyces pombe|chr 1|||Manual 29 0.66 SPCC1827.04 |||ankyrin repeat protein, unknown biological role|S... 27 2.0 SPCC622.15c |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 27 2.7 SPBC1861.02 |abp2||ARS binding protein Abp2|Schizosaccharomyces ... 27 2.7 SPAC513.05 |ams1||alpha-mannosidase |Schizosaccharomyces pombe|c... 27 2.7 SPBC354.10 |||RNAPII degradation factor |Schizosaccharomyces pom... 27 3.5 SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2 |Schiz... 26 4.7 SPAC688.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 6.1 SPAC24H6.09 |gef1||RhoGEF Gef1|Schizosaccharomyces pombe|chr 1||... 26 6.1 SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb... 25 8.1 SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|... 25 8.1 SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 25 8.1 SPCC24B10.07 |gad8||serine/threonine protein kinase Gad8 |Schizo... 25 8.1 SPAC3A12.05c |taf2||TATA-binding protein associated factor Taf2|... 25 8.1 SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 25 8.1 >SPAP7G5.05 |rpl1002|rpl10-2, rpl10|60S ribosomal protein L10|Schizosaccharomyces pombe|chr 1|||Manual Length = 221 Score = 142 bits (343), Expect = 6e-35 Identities = 61/75 (81%), Positives = 68/75 (90%) Frame = +3 Query: 30 MGRRPARCYRYCKNKPYPKSRFCRGVPDPKIRIFDLGKKRATVDDFPLCVHLVSDEYEQL 209 M RRPARCYRYCKNKPYPKSR+ R VPD KIRIFDLG+KRA VD+FPLC+HLVS+EYEQ+ Sbjct: 1 MARRPARCYRYCKNKPYPKSRYNRAVPDSKIRIFDLGRKRAGVDEFPLCIHLVSNEYEQI 60 Query: 210 SSEALEAGRICCNKY 254 +SEALEA RIC NKY Sbjct: 61 TSEALEAARICANKY 75 Score = 134 bits (325), Expect = 9e-33 Identities = 66/100 (66%), Positives = 76/100 (76%), Gaps = 1/100 (1%) Frame = +2 Query: 254 LVKNCGKDQFHIRMRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVRIGQPI 433 LVK GKD FH+R+R HPFHV+RINKMLSCAGADRLQTGMR AFGKP G VARV IGQ + Sbjct: 76 LVKIGGKDSFHLRVRAHPFHVVRINKMLSCAGADRLQTGMRHAFGKPNGLVARVNIGQVL 135 Query: 434 MSVRSSDRWKAQVIEALRRAKFKFPDVKRSTYQRS-GVSQ 550 MSVR+ D +A IEALRR ++KFP +R + G SQ Sbjct: 136 MSVRTKDSSRATAIEALRRCQYKFPGQQRIIVSKKWGFSQ 175 Score = 57.6 bits (133), Expect = 2e-09 Identities = 23/52 (44%), Positives = 34/52 (65%) Frame = +1 Query: 490 CQVQVPRRQKIYVSKKWGFTKYERDEFEKLREEGRLANDGCIVQYRPEHGPL 645 CQ + P +Q+I VSKKWGF++Y RDE+ + R G + DGC ++ + G L Sbjct: 155 CQYKFPGQQRIIVSKKWGFSQYARDEYIEKRSRGEIIPDGCYAKFLNKRGSL 206 >SPBC18E5.04 |rpl1001|rpl10-1, rpl10|60S ribosomal protein L10|Schizosaccharomyces pombe|chr 2|||Manual Length = 221 Score = 142 bits (343), Expect = 6e-35 Identities = 61/75 (81%), Positives = 68/75 (90%) Frame = +3 Query: 30 MGRRPARCYRYCKNKPYPKSRFCRGVPDPKIRIFDLGKKRATVDDFPLCVHLVSDEYEQL 209 M RRPARCYRYCKNKPYPKSR+ R VPD KIRIFDLG+KRA VD+FPLC+HLVS+EYEQ+ Sbjct: 1 MARRPARCYRYCKNKPYPKSRYNRAVPDSKIRIFDLGRKRAGVDEFPLCIHLVSNEYEQI 60 Query: 210 SSEALEAGRICCNKY 254 +SEALEA RIC NKY Sbjct: 61 TSEALEAARICANKY 75 Score = 134 bits (324), Expect = 1e-32 Identities = 66/100 (66%), Positives = 76/100 (76%), Gaps = 1/100 (1%) Frame = +2 Query: 254 LVKNCGKDQFHIRMRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVRIGQPI 433 LVK GKD FH+R+R HPFHV+RINKMLSCAGADRLQTGMR AFGKP G VARV IGQ + Sbjct: 76 LVKIGGKDSFHLRVRAHPFHVVRINKMLSCAGADRLQTGMRHAFGKPNGLVARVNIGQIL 135 Query: 434 MSVRSSDRWKAQVIEALRRAKFKFPDVKRSTYQRS-GVSQ 550 MSVR+ D +A IEALRR ++KFP +R + G SQ Sbjct: 136 MSVRTKDSSRATAIEALRRCQYKFPGQQRIIVSKKWGFSQ 175 Score = 57.6 bits (133), Expect = 2e-09 Identities = 23/52 (44%), Positives = 34/52 (65%) Frame = +1 Query: 490 CQVQVPRRQKIYVSKKWGFTKYERDEFEKLREEGRLANDGCIVQYRPEHGPL 645 CQ + P +Q+I VSKKWGF++Y RDE+ + R G + DGC ++ + G L Sbjct: 155 CQYKFPGQQRIIVSKKWGFSQYARDEYIEKRSRGEIIPDGCYAKFLNKRGSL 206 >SPBC1348.02 |||S. pombe specific 5Tm protein family|Schizosaccharomyces pombe|chr 2|||Manual Length = 344 Score = 29.5 bits (63), Expect = 0.50 Identities = 12/35 (34%), Positives = 20/35 (57%) Frame = -1 Query: 600 SEAALFTQLLKLITFILCETPLL*YVDLLTSGNLN 496 S+A F ++L FI C +P+L Y+ + GN + Sbjct: 263 SKARFFRRMLNAFIFIFCASPMLHYLSNIIFGNFD 297 >SPBPB2B2.19c |||S. pombe specific 5Tm protein family|Schizosaccharomyces pombe|chr 2|||Manual Length = 344 Score = 29.5 bits (63), Expect = 0.50 Identities = 12/35 (34%), Positives = 20/35 (57%) Frame = -1 Query: 600 SEAALFTQLLKLITFILCETPLL*YVDLLTSGNLN 496 S+A F ++L FI C +P+L Y+ + GN + Sbjct: 263 SKARFFRRMLNAFIFIFCASPMLHYLSNIIFGNFD 297 >SPAC977.01 |||S. pombe specific 5Tm protein family|Schizosaccharomyces pombe|chr 1||Partial|Manual Length = 316 Score = 29.5 bits (63), Expect = 0.50 Identities = 12/35 (34%), Positives = 20/35 (57%) Frame = -1 Query: 600 SEAALFTQLLKLITFILCETPLL*YVDLLTSGNLN 496 S+A F ++L FI C +P+L Y+ + GN + Sbjct: 235 SKARFFRRMLNAFIFIFCASPMLHYLSNIIFGNFD 269 >SPAC750.05c |||S. pombe specific 5Tm protein family|Schizosaccharomyces pombe|chr 1|||Manual Length = 344 Score = 29.5 bits (63), Expect = 0.50 Identities = 12/35 (34%), Positives = 20/35 (57%) Frame = -1 Query: 600 SEAALFTQLLKLITFILCETPLL*YVDLLTSGNLN 496 S+A F ++L FI C +P+L Y+ + GN + Sbjct: 263 SKARFFRRMLNAFIFIFCASPMLHYLSNIIFGNFD 297 >SPAC19D5.03 |cid1||poly|Schizosaccharomyces pombe|chr 1|||Manual Length = 405 Score = 29.1 bits (62), Expect = 0.66 Identities = 18/61 (29%), Positives = 32/61 (52%), Gaps = 1/61 (1%) Frame = +3 Query: 54 YRYCKNKPYPKSRFCRGVPDP-KIRIFDLGKKRATVDDFPLCVHLVSDEYEQLSSEALEA 230 Y+ N ++FC V + KI + +KRA +D LC+ +S + E ++ +LE+ Sbjct: 34 YQKVPNSHKEFTKFCYEVYNEIKISDKEFKEKRAALDTLRLCLKRISPDAELVAFGSLES 93 Query: 231 G 233 G Sbjct: 94 G 94 >SPCC1827.04 |||ankyrin repeat protein, unknown biological role|Schizosaccharomyces pombe|chr 3|||Manual Length = 600 Score = 27.5 bits (58), Expect = 2.0 Identities = 14/41 (34%), Positives = 23/41 (56%), Gaps = 1/41 (2%) Frame = +3 Query: 111 DPKIRIFDLGKKRATVDDFPLCV-HLVSDEYEQLSSEALEA 230 DPK+R+F KRAT + C LV+ + + S +++A Sbjct: 320 DPKLRVFPFTTKRATHSELLRCYKELVTPKISHVDSISIKA 360 >SPCC622.15c |||sequence orphan|Schizosaccharomyces pombe|chr 3|||Manual Length = 557 Score = 27.1 bits (57), Expect = 2.7 Identities = 15/39 (38%), Positives = 22/39 (56%), Gaps = 1/39 (2%) Frame = -1 Query: 276 SFPQFFTSTYCSRYVLPPKPLS-SAVHIRRTPSARTVES 163 SFPQ + ST + Y P P S S +++ P+A T+ S Sbjct: 466 SFPQNYDSTVPAVYPYMPPPFSGSDMNVMSNPAADTLRS 504 >SPBC1861.02 |abp2||ARS binding protein Abp2|Schizosaccharomyces pombe|chr 2|||Manual Length = 527 Score = 27.1 bits (57), Expect = 2.7 Identities = 29/86 (33%), Positives = 40/86 (46%) Frame = +1 Query: 67 KINRIRNRGSVGVYLIPRSVSSIWVRRERPLTTFHCACTWCPTNMNS*AQRLWRQDVSAA 246 KI R+R+ SV + L S+S W R L H T + + A RL + VS Sbjct: 340 KIKRLRSEPSVSLTL---SIS--WYERFEKL--MHAQNTMLRSAFSHVA-RLPMETVSQL 391 Query: 247 ISTRKELRKGSVPYPHETSPFPRYPH 324 +S E +P P ETSP P+ P+ Sbjct: 392 LSHYTETISQYLP-PSETSPIPKTPN 416 >SPAC513.05 |ams1||alpha-mannosidase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1077 Score = 27.1 bits (57), Expect = 2.7 Identities = 11/37 (29%), Positives = 22/37 (59%) Frame = +1 Query: 595 LANDGCIVQYRPEHGPLDAWRKFRLRSSMYNLINTFY 705 LA D ++Q + P + W+K++ R +++NTF+ Sbjct: 210 LAYDFLLMQQCVKQLPSNCWQKYKARQICNDIMNTFH 246 >SPBC354.10 |||RNAPII degradation factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 963 Score = 26.6 bits (56), Expect = 3.5 Identities = 11/25 (44%), Positives = 16/25 (64%) Frame = +1 Query: 226 RQDVSAAISTRKELRKGSVPYPHET 300 + +VSAA EL SVP+PH++ Sbjct: 320 KTNVSAAYEKPAELSSSSVPFPHKS 344 >SPAC23C4.10 |sec2||guanyl-nucleotide exchange factor Sec2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 527 Score = 26.2 bits (55), Expect = 4.7 Identities = 9/21 (42%), Positives = 13/21 (61%) Frame = +1 Query: 145 RERPLTTFHCACTWCPTNMNS 207 R RP +T H +CT+C + S Sbjct: 459 RSRPDSTSHASCTYCVARLRS 479 >SPAC688.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 1038 Score = 25.8 bits (54), Expect = 6.1 Identities = 11/20 (55%), Positives = 14/20 (70%) Frame = -3 Query: 403 STLRLAKRTTHPSLEPISSS 344 +T +LAKR T PSL P S+ Sbjct: 957 TTTKLAKRITQPSLSPARST 976 >SPAC24H6.09 |gef1||RhoGEF Gef1|Schizosaccharomyces pombe|chr 1|||Manual Length = 753 Score = 25.8 bits (54), Expect = 6.1 Identities = 9/18 (50%), Positives = 12/18 (66%) Frame = +1 Query: 628 PEHGPLDAWRKFRLRSSM 681 PE P+D WRK+ L + M Sbjct: 606 PEDKPVDTWRKYHLLAHM 623 >SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 2685 Score = 25.4 bits (53), Expect = 8.1 Identities = 12/38 (31%), Positives = 19/38 (50%) Frame = -2 Query: 143 LTQIEDTDLGIRYTPTEPRFRIRFIFAVPVASCWPAPH 30 L Q++++ L + T T P +R V SC+P H Sbjct: 408 LHQLQNSILRVNSTLTSPSLDLRLELLHDVLSCFPKKH 445 >SPAC328.01c ||SPAC3A11.01|karyopherin|Schizosaccharomyces pombe|chr 1|||Manual Length = 1234 Score = 25.4 bits (53), Expect = 8.1 Identities = 11/39 (28%), Positives = 21/39 (53%) Frame = -2 Query: 230 CLQSL*AQLFIFVGHQVHAQWKVVNGRSLLTQIEDTDLG 114 CL L L + H++ ++W+ +N R LL + + + G Sbjct: 940 CLLPLLPALLSELDHKLVSEWRRINDRGLLVEEDAEETG 978 >SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 3131 Score = 25.4 bits (53), Expect = 8.1 Identities = 14/35 (40%), Positives = 20/35 (57%) Frame = +2 Query: 272 KDQFHIRMRLHPFHVIRINKMLSCAGADRLQTGMR 376 +D H+RM ++ FH IN MLS + D + MR Sbjct: 859 EDNSHLRMSINSFH---INDMLSKSSRDNNRQLMR 890 >SPCC24B10.07 |gad8||serine/threonine protein kinase Gad8 |Schizosaccharomyces pombe|chr 3|||Manual Length = 569 Score = 25.4 bits (53), Expect = 8.1 Identities = 20/75 (26%), Positives = 32/75 (42%), Gaps = 8/75 (10%) Frame = -1 Query: 246 CSRYVLPP-KP-LSSAVHIRRTPSARTVESRQRS------LSSYPNRRYGSWDQVHPDRT 91 C++ + PP KP + SA+ S T E S LS +R+ +W P Sbjct: 494 CAKKIQPPFKPSVESAIDTSNFDSEFTSEIPMDSVVADSHLSETVQQRFANWSYQRPTTI 553 Query: 90 SISDTVYFCSTGSIL 46 SD + + GS++ Sbjct: 554 DTSDDINTIAPGSVI 568 >SPAC3A12.05c |taf2||TATA-binding protein associated factor Taf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1174 Score = 25.4 bits (53), Expect = 8.1 Identities = 8/15 (53%), Positives = 13/15 (86%) Frame = +1 Query: 97 VGVYLIPRSVSSIWV 141 +GVYLIP++ S +W+ Sbjct: 394 IGVYLIPKAWSDLWL 408 >SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces pombe|chr 1|||Manual Length = 1957 Score = 25.4 bits (53), Expect = 8.1 Identities = 12/26 (46%), Positives = 16/26 (61%) Frame = +3 Query: 132 DLGKKRATVDDFPLCVHLVSDEYEQL 209 ++ KKRA DD + +VS EYE L Sbjct: 1033 EIEKKRAENDDLQSKLSVVSSEYENL 1058 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 3,195,160 Number of Sequences: 5004 Number of extensions: 69382 Number of successful extensions: 239 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 229 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 239 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 333194204 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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