Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00675
(592 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g53540.1 68414.m06074 17.6 kDa class I small heat shock prote... 42 4e-04
At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein (HS... 40 0.001
At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein (HS... 36 0.015
At2g29500.1 68415.m03583 17.6 kDa class I small heat shock prote... 36 0.020
At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein (H... 35 0.035
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 33 0.11
At2g27980.1 68415.m03391 expressed protein 30 1.3
At1g78910.1 68414.m09199 pseudouridine synthase family protein l... 29 1.8
At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family prote... 29 3.1
At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containi... 27 7.1
At4g02400.1 68417.m00324 U3 ribonucleoprotein (Utp) family prote... 27 9.4
At3g46980.2 68416.m05102 transporter-related low similarity to b... 27 9.4
At3g46980.1 68416.m05101 transporter-related low similarity to b... 27 9.4
At2g01960.1 68415.m00131 expressed protein 27 9.4
>At1g53540.1 68414.m06074 17.6 kDa class I small heat shock protein
(HSP17.6C-CI) (AA 1-156) identical to (17.6 kDa class I
heat shock protein (HSP 17.6) (AA 1-156)(SP:P13853)
(GI:4376161) (Arabidopsis thaliana) (Nucleic Acids Res.
17 (19), 7995 (1989))
Length = 157
Score = 41.5 bits (93), Expect = 4e-04
Identities = 27/77 (35%), Positives = 43/77 (55%), Gaps = 8/77 (10%)
Frame = +3
Query: 303 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 458
D+ EE+ V+ DG I+ + G+ +EEK D+ + R +FTRR+ LPE E
Sbjct: 66 DLPGLRKEEVKVEVEDGNILQISGERSNENEEKNDKWHRVERSSGKFTRRFRLPENAKME 125
Query: 459 SVESRLSSDGVLSVIAP 509
+++ + +GVLSV P
Sbjct: 126 EIKASM-ENGVLSVTVP 141
>At3g46230.1 68416.m05004 17.4 kDa class I heat shock protein
(HSP17.4-CI) identical to 17.4 kDa class I heat shock
protein SP:P19036 from [Arabidopsis thaliana]
Length = 156
Score = 40.3 bits (90), Expect = 0.001
Identities = 28/77 (36%), Positives = 41/77 (53%), Gaps = 8/77 (10%)
Frame = +3
Query: 303 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 458
DV EE+ V+ DG I+ + G+ +EEK D + R +F RR+ LPE E
Sbjct: 65 DVPGLKKEEVKVEVEDGNILQISGERSSENEEKSDTWHRVERSSGKFMRRFRLPENAKVE 124
Query: 459 SVESRLSSDGVLSVIAP 509
V++ + +GVLSV P
Sbjct: 125 EVKASM-ENGVLSVTVP 140
>At5g59720.1 68418.m07486 18.1 kDa class I heat shock protein
(HSP18.1-CI) identical to 18.2 kDa class I heat shock
protein (HSP 18.2) (SP:P19037)[Arabidopsis thaliana];
contains Pfam profile: PF00011 Hsp20/alpha crystallin
family
Length = 161
Score = 36.3 bits (80), Expect = 0.015
Identities = 24/77 (31%), Positives = 42/77 (54%), Gaps = 8/77 (10%)
Frame = +3
Query: 303 DVQHFAPEEISVKTADGYIV-VEGK----HEEKKDQHGYISR---QFTRRYALPEGCTAE 458
D+ EE+ V+ D ++ + G+ +EEK D+ + R +F RR+ LPE E
Sbjct: 68 DLPGLKKEEVKVEVEDKNVLQISGERSKENEEKNDKWHRVERASGKFMRRFRLPENAKME 127
Query: 459 SVESRLSSDGVLSVIAP 509
V++ + +GVL+V+ P
Sbjct: 128 EVKATM-ENGVLTVVVP 143
>At2g29500.1 68415.m03583 17.6 kDa class I small heat shock protein
(HSP17.6B-CI) contains Pfam PF00011: Hsp20/alpha
crystallin family; identified in Scharf, K-D., et al,
Cell Stress & Chaperones (2001) 6: 225-237.
Length = 153
Score = 35.9 bits (79), Expect = 0.020
Identities = 25/77 (32%), Positives = 41/77 (53%), Gaps = 8/77 (10%)
Frame = +3
Query: 303 DVQHFAPEEISVKTA-DGYIVVEG-KHEEKKDQHGYISR------QFTRRYALPEGCTAE 458
D+ EE+ V+ D + + G +H EK+D++ R QFTRR+ LPE +
Sbjct: 62 DLPGLKKEEVKVEIEEDSVLKISGERHVEKEDKNDTWHRVERSSGQFTRRFRLPENVKMD 121
Query: 459 SVESRLSSDGVLSVIAP 509
V++ + +GVL+V P
Sbjct: 122 QVKAAM-ENGVLTVTVP 137
>At4g10250.1 68417.m01682 22.0 kDa ER small heat shock protein
(HSP22.0-ER) identical to endomembrane-localized small
heat shock protein GI:511795 from [Arabidopsis thaliana]
Length = 195
Score = 35.1 bits (77), Expect = 0.035
Identities = 25/85 (29%), Positives = 47/85 (55%), Gaps = 8/85 (9%)
Frame = +3
Query: 270 KSDKDKFQVNLDVQHFAPEEISVKTAD-GYIVVEG---KHEEKK-DQHGYISR---QFTR 425
K + ++ LD+ +E+ ++ + G + V G + EEKK DQ + R +F R
Sbjct: 76 KETAEGHEIMLDIPGLKKDEVKIEVEENGVLRVSGERKREEEKKGDQWHRVERSYGKFWR 135
Query: 426 RYALPEGCTAESVESRLSSDGVLSV 500
++ LP+ ESV+++L +GVL++
Sbjct: 136 QFKLPDNVDMESVKAKL-ENGVLTI 159
>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
(HSP17.8-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]; contains Pfam
PF00011: Hsp20/alpha crystallin family
Length = 157
Score = 33.5 bits (73), Expect = 0.11
Identities = 23/77 (29%), Positives = 41/77 (53%), Gaps = 8/77 (10%)
Frame = +3
Query: 303 DVQHFAPEEISVKTADGYIV-VEGKH----EEKKDQHGYISR---QFTRRYALPEGCTAE 458
D+ EE+ V+ D ++ + G+ EEK+D + R QF+R++ LPE +
Sbjct: 64 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMD 123
Query: 459 SVESRLSSDGVLSVIAP 509
V++ + +GVL+V P
Sbjct: 124 QVKASM-ENGVLTVTVP 139
>At2g27980.1 68415.m03391 expressed protein
Length = 1037
Score = 29.9 bits (64), Expect = 1.3
Identities = 17/48 (35%), Positives = 26/48 (54%)
Frame = +3
Query: 228 ASPCCRGSRLGSSIKSDKDKFQVNLDVQHFAPEEISVKTADGYIVVEG 371
A PC R +R S +K + K +VN D H PE+ ++ + D + V G
Sbjct: 203 AKPCKRLTR--SMLKVEGIKSEVNADDDHVNPEKDAIGSEDNCVDVSG 248
>At1g78910.1 68414.m09199 pseudouridine synthase family protein low
similarity to SP|P23851 Ribosomal large subunit
pseudouridine synthase C (EC 4.2.1.70) (Pseudouridylate
synthase) (Uracil hydrolyase) {Escherichia coli};
contains Pfam profile PF00849: RNA pseudouridylate
synthase
Length = 478
Score = 29.5 bits (63), Expect = 1.8
Identities = 30/93 (32%), Positives = 37/93 (39%), Gaps = 2/93 (2%)
Frame = -2
Query: 468 TPQIQPYSPPAKRSDE*IDAICNHADLSSPRVCLQPR--CSRPPF*QRFLPGRNAGRPGL 295
T I+ YS A I N A L P+ PR S PPF LPG+NAG G
Sbjct: 14 TVLIRRYSRVAPPPSSVIRVTNNVAHLGPPKQGPLPRQLISLPPFPGHPLPGKNAGADGD 73
Query: 294 LGTYPCHF*CWSQVSSRGSKVTPRAVVFSAHKG 196
G H S V ++ +A+ KG
Sbjct: 74 DGDSGGHVTAISWVKYYFEEIYDKAIQTHFTKG 106
>At5g08600.1 68418.m01023 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 822
Score = 28.7 bits (61), Expect = 3.1
Identities = 17/54 (31%), Positives = 23/54 (42%)
Frame = +3
Query: 324 EEISVKTADGYIVVEGKHEEKKDQHGYISRQFTRRYALPEGCTAESVESRLSSD 485
+EI D Y EG EE+ ++ R + L E C E+VES D
Sbjct: 48 DEIDSAYGDFYEYEEGVPEEESRKNNRYERVVNYEFELAEDCEDENVESEDDDD 101
>At1g73710.1 68414.m08535 pentatricopeptide (PPR) repeat-containing
protein low similarity to fertility restorer [Petunia x
hybrida] GI:22128587, post-transcriptional control of
chloroplast gene expression CRP1 [Zea mays] GI:3289002;
contains Pfam profile PF01535: PPR repeat
Length = 991
Score = 27.5 bits (58), Expect = 7.1
Identities = 15/48 (31%), Positives = 20/48 (41%)
Frame = -2
Query: 489 HHLKTAGTPQIQPYSPPAKRSDE*IDAICNHADLSSPRVCLQPRCSRP 346
HHL + ++ P S P S + H SS PRCS+P
Sbjct: 26 HHLALSSKARVFPLSLPCNFSSRVSFKLQLHCAASSSSSVSPPRCSKP 73
>At4g02400.1 68417.m00324 U3 ribonucleoprotein (Utp) family protein
contains Pfam profile: PF04615 Utp14 protein
Length = 858
Score = 27.1 bits (57), Expect = 9.4
Identities = 17/50 (34%), Positives = 22/50 (44%)
Frame = +3
Query: 339 KTADGYIVVEGKHEEKKDQHGYISRQFTRRYALPEGCTAESVESRLSSDG 488
+ D Y EG EE+ ++ R Y LPE E+VES DG
Sbjct: 52 ENVDLYEYEEGVPEEESKKNNRYDRVDNYDYELPEDFEDENVESDDDEDG 101
>At3g46980.2 68416.m05102 transporter-related low similarity to
brain specific Na+-dependent inorganic phosphate
cotransporter from [Rattus norvegicus] GI:507415, [Homo
sapiens] GI:7328925, vesicular glutamate transporter 3
from [Rattus norvegicus] GI:21685382; contains Pfam
profile PF00083: major facilitator superfamily protein
Length = 469
Score = 27.1 bits (57), Expect = 9.4
Identities = 16/54 (29%), Positives = 23/54 (42%)
Frame = +2
Query: 377 RGEERSAWLHIASIHSSLRFAGGLYG*ICGVPAVFRWCVVCNRTRKVPPAVEGE 538
R R L + +SL+F G +CG+P R V C+ R+ P E
Sbjct: 44 RDRRRKLVLCTGRVVNSLKFTGNTSVDLCGIPR-HRLRVSCSDARRTPEETAAE 96
>At3g46980.1 68416.m05101 transporter-related low similarity to
brain specific Na+-dependent inorganic phosphate
cotransporter from [Rattus norvegicus] GI:507415, [Homo
sapiens] GI:7328925, vesicular glutamate transporter 3
from [Rattus norvegicus] GI:21685382; contains Pfam
profile PF00083: major facilitator superfamily protein
Length = 533
Score = 27.1 bits (57), Expect = 9.4
Identities = 16/54 (29%), Positives = 23/54 (42%)
Frame = +2
Query: 377 RGEERSAWLHIASIHSSLRFAGGLYG*ICGVPAVFRWCVVCNRTRKVPPAVEGE 538
R R L + +SL+F G +CG+P R V C+ R+ P E
Sbjct: 44 RDRRRKLVLCTGRVVNSLKFTGNTSVDLCGIPR-HRLRVSCSDARRTPEETAAE 96
>At2g01960.1 68415.m00131 expressed protein
Length = 260
Score = 27.1 bits (57), Expect = 9.4
Identities = 10/24 (41%), Positives = 15/24 (62%)
Frame = +3
Query: 171 RFSQCCSRPPCEQRILPPVASPCC 242
RF + PP ++R+LP V + CC
Sbjct: 147 RFVNSYALPPYDRRLLPSVKTGCC 170
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,364,722
Number of Sequences: 28952
Number of extensions: 288682
Number of successful extensions: 770
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 751
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 770
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1171109464
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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