Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00670
(623 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23H4.09 |cdb4||curved DNA-binding protein Cdb4|Schizosacchar... 73 4e-14
SPBC23E6.05 |arx1||ribosomal export complex Arx1 |Schizosaccharo... 67 2e-12
SPBC14C8.03 |fma2||methionine aminopeptidase Fma2 |Schizosacchar... 35 0.011
SPAC343.11c |msc1||multi-copy suppressor of Chk1 |Schizosaccharo... 26 3.8
SPAC922.04 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||... 26 3.8
SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 26 5.1
SPAC6G9.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr ... 26 5.1
SPAC25H1.09 |mde5|meu30, SPAC4A8.01|alpha-amylase homolog Mde5|S... 26 5.1
SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyc... 25 6.7
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 25 6.7
SPAC17C9.09c |tim13||TIM22 inner membrane protein import complex... 25 6.7
SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 25 6.7
SPBC30D10.11 |gpi1||pig-Q|Schizosaccharomyces pombe|chr 2|||Manual 25 8.9
>SPAC23H4.09 |cdb4||curved DNA-binding protein
Cdb4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 381
Score = 72.5 bits (170), Expect = 4e-14
Identities = 36/93 (38%), Positives = 57/93 (61%)
Frame = +1
Query: 4 TSEKEVEKTIAEDLVVTKYKLAGQIVNRVLEQVIAKCVPDASAREICEFGDKLVLEETNK 183
TSE V+ +++ V KYK+AG++ V+++V+ C P A +IC GD+L+ E K
Sbjct: 7 TSETAVDYSLSNPETVNKYKIAGEVSQNVIKKVVELCQPGAKIYDICVRGDELLNEAIKK 66
Query: 184 VFKKEKDSKKGIAFSTCVSVNNCIVTFRPLRAN 282
V+ + KD+ KGIAF T VS N+ PL+++
Sbjct: 67 VY-RTKDAYKGIAFPTAVSPNDMAAHLSPLKSD 98
Score = 61.3 bits (142), Expect = 1e-10
Identities = 31/54 (57%), Positives = 38/54 (70%), Gaps = 2/54 (3%)
Frame = +3
Query: 258 HFSPIASEPD--YILKKGDLAKIDLGAHIDGFIAVVAHTVVVGESEVSGRAADV 413
H SP+ S+P+ LK GD+ KI LGAHIDGF ++VA T VV E V+G AADV
Sbjct: 91 HLSPLKSDPEANLALKSGDVVKILLGAHIDGFASLVATTTVVSEEPVTGPAADV 144
Score = 60.5 bits (140), Expect = 2e-10
Identities = 24/46 (52%), Positives = 34/46 (73%)
Frame = +1
Query: 457 LKPGTENYAVTEAIQKISAEYGCKPIEGMLSHQLKQFRIDGEKSII 594
+KPG N+ VT+ + KI+ YGCKP+ GMLSHQ ++ IDG+K +I
Sbjct: 159 IKPGNTNWQVTDIVDKIATSYGCKPVAGMLSHQQEREVIDGKKQVI 204
>SPBC23E6.05 |arx1||ribosomal export complex Arx1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 417
Score = 66.9 bits (156), Expect = 2e-12
Identities = 30/73 (41%), Positives = 47/73 (64%)
Frame = +1
Query: 52 TKYKLAGQIVNRVLEQVIAKCVPDASAREICEFGDKLVLEETNKVFKKEKDSKKGIAFST 231
+KY+ AG +V++ QV ++CVP AS REI +GD L+ E + ++K ++ +KGIA T
Sbjct: 19 SKYRDAGALVSKAFHQVASRCVPGASTREISSYGDNLLHEYKSSIYKSQR-FEKGIAEPT 77
Query: 232 CVSVNNCIVTFRP 270
+ VNNC + P
Sbjct: 78 SICVNNCAYNYAP 90
Score = 42.3 bits (95), Expect = 5e-05
Identities = 21/66 (31%), Positives = 34/66 (51%)
Frame = +3
Query: 180 QGFQKGERLQERHCIFNMRFCEQLHCHFSPIASEPDYILKKGDLAKIDLGAHIDGFIAVV 359
Q F+KG C+ N + ++ Y L+ GD+ KI +G H DG+ A++
Sbjct: 67 QRFEKGIAEPTSICVNNCAYNYAPGPESVIAGNDNSYHLQVGDVTKISMGLHFDGYTALI 126
Query: 360 AHTVVV 377
+HT+VV
Sbjct: 127 SHTIVV 132
>SPBC14C8.03 |fma2||methionine aminopeptidase Fma2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 426
Score = 34.7 bits (76), Expect = 0.011
Identities = 17/42 (40%), Positives = 27/42 (64%), Gaps = 2/42 (4%)
Frame = +3
Query: 252 HC--HFSPIASEPDYILKKGDLAKIDLGAHIDGFIAVVAHTV 371
HC H++P A + ILK+ D+ K+D+G H++G I A T+
Sbjct: 175 HCAAHYTPNAGDTT-ILKEKDVMKVDIGVHVNGRIVDSAFTM 215
>SPAC343.11c |msc1||multi-copy suppressor of Chk1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1588
Score = 26.2 bits (55), Expect = 3.8
Identities = 13/32 (40%), Positives = 17/32 (53%)
Frame = +2
Query: 113 AFQTHQQEKYVNLAINLYWRRLTRFSKRRKTP 208
AF T +Q Y N NLY+ ++ S R TP
Sbjct: 474 AFPTSRQNAYYNDPWNLYFIHFSKLSPLRFTP 505
>SPAC922.04 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 117
Score = 26.2 bits (55), Expect = 3.8
Identities = 12/41 (29%), Positives = 19/41 (46%)
Frame = -1
Query: 449 KSRFTSMMSCQQNICSTSRDFTFTHNYCMSYNSYKSINVST 327
K+ + M + C S +T + N C +Y + SIN T
Sbjct: 32 KTIYFGMKAYDDVNCKGSTSYTISFNKCQAYPNINSINALT 72
>SPBC3H7.05c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 357
Score = 25.8 bits (54), Expect = 5.1
Identities = 9/24 (37%), Positives = 14/24 (58%)
Frame = -1
Query: 371 YCMSYNSYKSINVSTKIYLG*ISF 300
YC +YKS+ + T +Y+G F
Sbjct: 144 YCRKVLTYKSLGIRTAVYVGAFCF 167
>SPAC6G9.14 |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 681
Score = 25.8 bits (54), Expect = 5.1
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = -3
Query: 162 KFIAKFTYFSC*CVWNAFCDDLLKDSVHYLSC*LV 58
K + KF+ C +++A C+D L S H C +V
Sbjct: 472 KCLNKFSQEDCQFIFDAICEDPLDVSTHRHGCCVV 506
>SPAC25H1.09 |mde5|meu30, SPAC4A8.01|alpha-amylase homolog
Mde5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 513
Score = 25.8 bits (54), Expect = 5.1
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = +1
Query: 553 QLKQFRIDGEKSIITKSIRST 615
Q+KQF+IDG + TK +R T
Sbjct: 214 QVKQFKIDGLRVDATKHVRRT 234
>SPBC336.03 |efc25||exchange factor Cdc25p-like|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 987
Score = 25.4 bits (53), Expect = 6.7
Identities = 12/32 (37%), Positives = 17/32 (53%)
Frame = -3
Query: 276 SQWAKSDNAVVHRNAC*KCNAFLGVFLLFENL 181
S W K DN V +N C N ++ + FE+L
Sbjct: 655 SSWEKGDNFVTKKNVCTVMNLWVQKY-FFEDL 685
>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1044
Score = 25.4 bits (53), Expect = 6.7
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = +1
Query: 124 ASAREICEFGDKLVLEETNKVFKKEKD 204
++ +E FG+KL E T K+ EKD
Sbjct: 865 SNLQEKINFGNKLAKEHTEKISSLEKD 891
>SPAC17C9.09c |tim13||TIM22 inner membrane protein import complex
subunit Tim13|Schizosaccharomyces pombe|chr 1|||Manual
Length = 95
Score = 25.4 bits (53), Expect = 6.7
Identities = 10/33 (30%), Positives = 21/33 (63%)
Frame = +1
Query: 25 KTIAEDLVVTKYKLAGQIVNRVLEQVIAKCVPD 123
K I ++L V + AG++++++ E KC+P+
Sbjct: 23 KQIRQELAVAQ---AGELISKINENCFDKCIPE 52
>SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 486
Score = 25.4 bits (53), Expect = 6.7
Identities = 12/32 (37%), Positives = 17/32 (53%)
Frame = +3
Query: 279 EPDYILKKGDLAKIDLGAHIDGFIAVVAHTVV 374
EP+Y+L G+L+ +L ID F T V
Sbjct: 382 EPEYLLMYGNLSNEELVERIDAFYMNARRTYV 413
>SPBC30D10.11 |gpi1||pig-Q|Schizosaccharomyces pombe|chr 2|||Manual
Length = 653
Score = 25.0 bits (52), Expect = 8.9
Identities = 25/82 (30%), Positives = 40/82 (48%)
Frame = -3
Query: 381 HPQLLYELQQL*IHQCEHQDLSWLNLLFSECNPVRSQWAKSDNAVVHRNAC*KCNAFLGV 202
H Q+L L++L +H ++ SW L S + +Q + + H N K N++ V
Sbjct: 167 HQQIL--LKKLKLHFPRRKENSWKRSLRSGLIELLNQ-SFEVRMLTHENNN-KKNSY--V 220
Query: 201 FLLFENLVSLLQYKFIAKFTYF 136
F LF+ + S Y F + F YF
Sbjct: 221 FRLFDRVSSSTFYFFNSLFAYF 242
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,541,553
Number of Sequences: 5004
Number of extensions: 50596
Number of successful extensions: 168
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 157
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 166
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 275671126
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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