Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00669
(782 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g55260.1 68416.m06137 glycosyl hydrolase family 20 protein si... 73 2e-13
At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein si... 73 3e-13
At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein co... 63 2e-10
At1g67120.1 68414.m07636 midasin-related similar to Midasin (MID... 28 6.1
At1g63780.1 68414.m07218 brix domain-containing protein contains... 28 6.1
At5g57420.1 68418.m07173 auxin-responsive factor-related contain... 28 8.0
At5g49160.1 68418.m06085 DNA (cytosine-5-)-methyltransferase (AT... 28 8.0
At2g43160.3 68415.m05361 epsin N-terminal homology (ENTH) domain... 28 8.0
At2g43160.2 68415.m05360 epsin N-terminal homology (ENTH) domain... 28 8.0
At2g43160.1 68415.m05359 epsin N-terminal homology (ENTH) domain... 28 8.0
>At3g55260.1 68416.m06137 glycosyl hydrolase family 20 protein
similar to beta-hexosaminidase A SP:P13723 from
[Dictyostelium discoideum]
Length = 541
Score = 72.9 bits (171), Expect = 2e-13
Identities = 34/82 (41%), Positives = 53/82 (64%), Gaps = 1/82 (1%)
Frame = +2
Query: 539 ATIRGNSFFGVRHGLETLSQLIVYDDIRNNLLIVRDVT-IKDRPVYPYRGILLDTARNFY 715
ATI N+ +G GLET SQL +D I ++ I + I+D+P + YRG+L+DT+R++
Sbjct: 140 ATIEANTVYGALRGLETFSQLCAFDYITKSVQIYKAPWYIQDKPRFGYRGLLIDTSRHYL 199
Query: 716 SIESIKRTIDAMAAVNLNTFHW 781
I+ IK+ I++M+ LN HW
Sbjct: 200 PIDVIKQIIESMSFAKLNVLHW 221
>At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein
similar to beta-hexosaminidase precursor SP:P43077 from
[Candida albicans]
Length = 580
Score = 72.5 bits (170), Expect = 3e-13
Identities = 35/82 (42%), Positives = 53/82 (64%)
Frame = +2
Query: 536 NATIRGNSFFGVRHGLETLSQLIVYDDIRNNLLIVRDVTIKDRPVYPYRGILLDTARNFY 715
+A + +S +G GLET SQ+I +L + + I+D P++ +RG+LLDT+RN+Y
Sbjct: 128 SAHLLAHSAWGAMRGLETFSQMIW--GTSPDLCLPVGIYIQDSPLFGHRGVLLDTSRNYY 185
Query: 716 SIESIKRTIDAMAAVNLNTFHW 781
++ I RTI AM+A LN FHW
Sbjct: 186 GVDDIMRTIKAMSANKLNVFHW 207
>At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein
contains Pfam PF00728: Glycosyl hydrolase family 20,
catalytic domain; contains Pfam PF02838: Glycosyl
hydrolase family 20, domain 2; similar to
Beta-hexosaminidase beta chain precursor (EC 3.2.1.52)
(N-acetyl-beta- glucosaminidase)
(Beta-N-acetylhexosaminidase) (Hexosaminidase B)
(Swiss-Prot:P07686) [Homo sapiens]
Length = 535
Score = 62.9 bits (146), Expect = 2e-10
Identities = 29/82 (35%), Positives = 48/82 (58%), Gaps = 1/82 (1%)
Frame = +2
Query: 539 ATIRGNSFFGVRHGLETLSQLIVYDDIRNNL-LIVRDVTIKDRPVYPYRGILLDTARNFY 715
A + S +G HGL+T SQL ++ + + +++ I D+P + YRG+L+DT+R++
Sbjct: 137 AQLEAKSVYGALHGLQTFSQLCHFNLKKKVIEILMTPWNIIDQPRFSYRGLLIDTSRHYL 196
Query: 716 SIESIKRTIDAMAAVNLNTFHW 781
+ IK ID+M LN HW
Sbjct: 197 PLPVIKNVIDSMTYAKLNVLHW 218
>At1g67120.1 68414.m07636 midasin-related similar to Midasin
(MIDAS-containing protein) (Swiss-Prot:Q12019)
[Saccharomyces cerevisiae]; similar to Midasin
(MIDAS-containing protein) (Swiss-Prot:Q9NU22) [Homo
sapiens]; contains Prosite PS00017: ATP/GTP-binding site
motif A (P-loop)
Length = 5336
Score = 28.3 bits (60), Expect = 6.1
Identities = 16/62 (25%), Positives = 33/62 (53%), Gaps = 5/62 (8%)
Frame = -2
Query: 715 VEVPRGVQQNTSIRIDRPVLNCD---VTDNQQIIPDVVVH--D*LRERLQTVTDAEERVS 551
+E+ + QN+ +DR +L+C+ +T + + V H LR+ +TD E ++S
Sbjct: 4228 IEILIPIAQNSKASLDRLLLDCNGFIITPSSSLKQFVTQHMVQVLRQNFDQLTDLENQIS 4287
Query: 550 TY 545
++
Sbjct: 4288 SF 4289
>At1g63780.1 68414.m07218 brix domain-containing protein contains
Pfam domain, PF04427: Brix domain
Length = 294
Score = 28.3 bits (60), Expect = 6.1
Identities = 15/35 (42%), Positives = 18/35 (51%)
Frame = +1
Query: 340 TTSSQRLPTGLRHWFPVPCLKASRRRLQENQSQFI 444
T QR+ L+H FP P L A R NQS +I
Sbjct: 196 TQMGQRVGNILKHIFPAPKLDAKRIVTFSNQSDYI 230
>At5g57420.1 68418.m07173 auxin-responsive factor-related contains
weak similarity to Swiss-Prot:P33078 auxin-responsive
protein IAA5 (Indoleacetic acid-induced protein 5)
(Auxin-induced protein AUX2-27) [Arabidopsis thaliana]
Length = 171
Score = 27.9 bits (59), Expect = 8.0
Identities = 11/21 (52%), Positives = 16/21 (76%)
Frame = +2
Query: 599 LIVYDDIRNNLLIVRDVTIKD 661
LI Y+D+ N+LL+ D+T KD
Sbjct: 127 LIAYEDMENDLLLAGDLTWKD 147
>At5g49160.1 68418.m06085 DNA (cytosine-5-)-methyltransferase (ATHIM)
identical to SP|P34881 DNA (cytosine-5)-methyltransferase
AthI (EC 2.1.1.37) {Arabidopsis thaliana}
Length = 1534
Score = 27.9 bits (59), Expect = 8.0
Identities = 13/40 (32%), Positives = 23/40 (57%), Gaps = 3/40 (7%)
Frame = -3
Query: 276 YPDLSRSWFRPE---ETIIVTEHLAGFQTEDRLLILRLVP 166
Y +++ SWF+ E TI +T+H+ E L+ +L+P
Sbjct: 1359 YKEVAVSWFQKEIRGNTIALTDHICKAMNELNLIRCKLIP 1398
>At2g43160.3 68415.m05361 epsin N-terminal homology (ENTH)
domain-containing protein contains Pfam PF01417: ENTH
domain. ENTH (Epsin N-terminal homology) domain; similar
to Af10-protein (GI:1724114) [Avena fatua]
Length = 895
Score = 27.9 bits (59), Expect = 8.0
Identities = 11/35 (31%), Positives = 22/35 (62%)
Frame = +1
Query: 670 LSLSRYFVGHREELLLHRINQKNYRRNGRSEFKHI 774
L++ Y VGH E ++ I ++ Y+ + S+F++I
Sbjct: 84 LTVLEYMVGHGSERVIDEIRERAYQISTLSDFQYI 118
>At2g43160.2 68415.m05360 epsin N-terminal homology (ENTH)
domain-containing protein contains Pfam PF01417: ENTH
domain. ENTH (Epsin N-terminal homology) domain; similar
to Af10-protein (GI:1724114) [Avena fatua]
Length = 895
Score = 27.9 bits (59), Expect = 8.0
Identities = 11/35 (31%), Positives = 22/35 (62%)
Frame = +1
Query: 670 LSLSRYFVGHREELLLHRINQKNYRRNGRSEFKHI 774
L++ Y VGH E ++ I ++ Y+ + S+F++I
Sbjct: 84 LTVLEYMVGHGSERVIDEIRERAYQISTLSDFQYI 118
>At2g43160.1 68415.m05359 epsin N-terminal homology (ENTH)
domain-containing protein contains Pfam PF01417: ENTH
domain. ENTH (Epsin N-terminal homology) domain; similar
to Af10-protein (GI:1724114) [Avena fatua]
Length = 895
Score = 27.9 bits (59), Expect = 8.0
Identities = 11/35 (31%), Positives = 22/35 (62%)
Frame = +1
Query: 670 LSLSRYFVGHREELLLHRINQKNYRRNGRSEFKHI 774
L++ Y VGH E ++ I ++ Y+ + S+F++I
Sbjct: 84 LTVLEYMVGHGSERVIDEIRERAYQISTLSDFQYI 118
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,013,646
Number of Sequences: 28952
Number of extensions: 367012
Number of successful extensions: 1015
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 980
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1013
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1755792000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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