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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00657
         (616 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ...   186   2e-48
SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p...   185   4e-48
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M...   147   1e-36
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce...   129   3e-31
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc...    98   8e-22
SPCC1235.05c |fft2||fun thirty related protein Fft2|Schizosaccha...    33   0.033
SPBC16C6.05 |||translation initiation factor |Schizosaccharomyce...    30   0.23 
SPCC663.03 |pmd1||leptomycin efflux transporter Pmd1|Schizosacch...    29   0.53 
SPBC16E9.13 |ksp1|ppk20|serine/threonine protein kinase Ksp1 |Sc...    28   1.2  
SPBC3B9.02c |cwf28||splicing factor Cwf28|Schizosaccharomyces po...    27   1.6  
SPBC1734.01c ||SPBC337.17c|RNA-binding protein|Schizosaccharomyc...    27   2.8  
SPAC20G4.08 ||SPAC4F10.01|sequence orphan|Schizosaccharomyces po...    27   2.8  
SPAC688.11 |end4|sla2|Huntingtin-interacting protein homolog|Sch...    26   5.0  
SPAC1556.07 |pmm1||phosphomannomutase Pmm1|Schizosaccharomyces p...    25   6.6  
SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pom...    25   6.6  
SPAC22F8.10c |sap145||U2 snRNP-associated protein Sap145 |Schizo...    25   6.6  
SPAC2F7.03c |pom1||DYRK family protein kinase Pom1|Schizosacchar...    25   6.6  
SPBC16G5.16 |||transcription factor zf-fungal binuclear cluster ...    25   6.6  
SPCC126.03 |pus1|SPCC126.03, SPCC126.03|tRNA pseudouridylate syn...    25   6.6  
SPBC2F12.05c |||sterol binding ankyrin repeat protein|Schizosacc...    25   8.7  
SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|ch...    25   8.7  
SPBC1652.01 |||conserved fungal protein|Schizosaccharomyces pomb...    25   8.7  

>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 644

 Score =  186 bits (453), Expect = 2e-48
 Identities = 92/133 (69%), Positives = 105/133 (78%)
 Frame = +1

Query: 118 VMTTLIKRNTTIPTKQNQTFTTYSDNQPGVLIQVFEGERAMTKDNNCSVNSS*PGSHRRX 297
           VMT LIKRNTTIPTK+++ F+TY+DNQPGVLIQVFEGERA TKD N        G     
Sbjct: 407 VMTPLIKRNTTIPTKKSEVFSTYADNQPGVLIQVFEGERARTKDCNLLGKFELSGIPPAP 466

Query: 298 DGVPQIEVTFDIDANGILNVSAIEKSTNKENKITITNDKGRLSKEEIERMVNEAEKYRNE 477
            GVPQIEVTFD+DANGILNVSA+EK T K  KITITNDKGRLSKEEI+RMV+EAEKY+ E
Sbjct: 467 RGVPQIEVTFDVDANGILNVSALEKGTGKTQKITITNDKGRLSKEEIDRMVSEAEKYKAE 526

Query: 478 DDKQKETIQARMH 516
           D+ +   IQA+ H
Sbjct: 527 DEAETSRIQAKNH 539



 Score = 40.7 bits (91), Expect = 2e-04
 Identities = 34/97 (35%), Positives = 37/97 (38%)
 Frame = +3

Query: 9   AAVQAAILHGDKSEEVQDXXXXXXXXXXXGIETAGXCXXXXXXXXXXXXXXTESDIHHLL 188
           AAVQAAIL GD SE+ QD           GIETAG                         
Sbjct: 371 AAVQAAILVGDTSEKTQDLLLLDVAPLSLGIETAGGVMTPLIKRNTTIPTKKSEVFSTYA 430

Query: 189 **PTRSTHPSI*G*ACYDQR*QLLGKFELTGIPPAXR 299
                       G     +   LLGKFEL+GIPPA R
Sbjct: 431 DNQPGVLIQVFEGERARTKDCNLLGKFELSGIPPAPR 467



 Score = 38.3 bits (85), Expect = 9e-04
 Identities = 14/32 (43%), Positives = 25/32 (78%)
 Frame = +3

Query: 507 QNALESYCFSMKSTMEDEKLKEKISDSDKQTI 602
           +N LESY +S++++++D  LK+K+  SDK+ I
Sbjct: 537 KNHLESYAYSLRNSLDDPNLKDKVDASDKEAI 568


>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 647

 Score =  185 bits (451), Expect = 4e-48
 Identities = 93/133 (69%), Positives = 104/133 (78%)
 Frame = +1

Query: 118 VMTTLIKRNTTIPTKQNQTFTTYSDNQPGVLIQVFEGERAMTKDNNCSVNSS*PGSHRRX 297
           VMT LIKRNTTIPTK+++ F+TYSDNQPGVLIQVFEGERA TKD N        G     
Sbjct: 407 VMTPLIKRNTTIPTKKSEIFSTYSDNQPGVLIQVFEGERARTKDCNLLGKFELSGIPPAP 466

Query: 298 DGVPQIEVTFDIDANGILNVSAIEKSTNKENKITITNDKGRLSKEEIERMVNEAEKYRNE 477
            GVPQIEVTFD+DANGILNVSA+EK T K  KITITNDKGRLSKEEI+RMV EAEKY+ E
Sbjct: 467 RGVPQIEVTFDVDANGILNVSALEKGTGKTQKITITNDKGRLSKEEIDRMVAEAEKYKAE 526

Query: 478 DDKQKETIQARMH 516
           D+ +   IQA+ H
Sbjct: 527 DEAESGRIQAKNH 539



 Score = 40.3 bits (90), Expect = 2e-04
 Identities = 33/97 (34%), Positives = 37/97 (38%)
 Frame = +3

Query: 9   AAVQAAILHGDKSEEVQDXXXXXXXXXXXGIETAGXCXXXXXXXXXXXXXXTESDIHHLL 188
           AAVQAA+L GD SE+ QD           GIETAG                         
Sbjct: 371 AAVQAAVLTGDTSEKTQDLLLLDVAPLSMGIETAGGVMTPLIKRNTTIPTKKSEIFSTYS 430

Query: 189 **PTRSTHPSI*G*ACYDQR*QLLGKFELTGIPPAXR 299
                       G     +   LLGKFEL+GIPPA R
Sbjct: 431 DNQPGVLIQVFEGERARTKDCNLLGKFELSGIPPAPR 467



 Score = 39.9 bits (89), Expect = 3e-04
 Identities = 14/32 (43%), Positives = 26/32 (81%)
 Frame = +3

Query: 507 QNALESYCFSMKSTMEDEKLKEKISDSDKQTI 602
           +N LESY +S++++++D  LK+K+  SDK+T+
Sbjct: 537 KNHLESYAYSLRNSLDDPNLKDKVDASDKETV 568


>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 663

 Score =  147 bits (357), Expect = 1e-36
 Identities = 77/132 (58%), Positives = 94/132 (71%), Gaps = 1/132 (0%)
 Frame = +1

Query: 118 VMTTLIKRNTTIPTKQNQTFTTYSDNQPGVLIQVFEGERAMTKDNNCSVNSS*PGSHRRX 297
           VMT LI RNT IPT+++Q F+T  DNQ  VLIQV+EGER +TKDNN        G     
Sbjct: 436 VMTKLIGRNTPIPTRKSQIFSTAVDNQNTVLIQVYEGERTLTKDNNLLGKFDLRGIPPAP 495

Query: 298 DGVPQIEVTFDIDANGILNVSAIEKS-TNKENKITITNDKGRLSKEEIERMVNEAEKYRN 474
            GVPQIEVTF++DANG+L VSA++KS   K  K+ I NDKGRLS+E+IERMV EAE++  
Sbjct: 496 RGVPQIEVTFEVDANGVLTVSAVDKSGKGKPEKLVIKNDKGRLSEEDIERMVKEAEEFAE 555

Query: 475 EDDKQKETIQAR 510
           ED   KE I+AR
Sbjct: 556 EDKILKERIEAR 567



 Score = 34.7 bits (76), Expect = 0.011
 Identities = 15/35 (42%), Positives = 23/35 (65%), Gaps = 1/35 (2%)
 Frame = +3

Query: 507 QNALESYCFSMKSTMED-EKLKEKISDSDKQTILD 608
           +N LE+Y +S+K   +D E+L  K+   DKQ +LD
Sbjct: 567 RNTLENYAYSLKGQFDDDEQLGGKVDPEDKQAVLD 601


>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 674

 Score =  129 bits (312), Expect = 3e-31
 Identities = 67/140 (47%), Positives = 90/140 (64%)
 Frame = +1

Query: 85  PFPSVLRLLDXVMTTLIKRNTTIPTKQNQTFTTYSDNQPGVLIQVFEGERAMTKDNNCSV 264
           P    +  L  V T LI RNTTIPT+++Q F+T +D Q  V I+VF+GER + +DN    
Sbjct: 439 PLSLGIETLGGVFTRLINRNTTIPTRKSQVFSTAADGQTAVEIRVFQGERELVRDNKLIG 498

Query: 265 NSS*PGSHRRXDGVPQIEVTFDIDANGILNVSAIEKSTNKENKITITNDKGRLSKEEIER 444
           N    G      G PQIEV+FD+DA+GI+NVSA +K+TNK++ IT+    G L+  EIE 
Sbjct: 499 NFQLTGIAPAPKGQPQIEVSFDVDADGIINVSARDKATNKDSSITVAGSSG-LTDSEIEA 557

Query: 445 MVNEAEKYRNEDDKQKETIQ 504
           MV +AEKYR  D  +KE I+
Sbjct: 558 MVADAEKYRASDMARKEAIE 577


>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 613

 Score = 98.3 bits (234), Expect = 8e-22
 Identities = 49/131 (37%), Positives = 75/131 (57%)
 Frame = +1

Query: 118 VMTTLIKRNTTIPTKQNQTFTTYSDNQPGVLIQVFEGERAMTKDNNCSVNSS*PGSHRRX 297
           V   +  RNT IPT + +TFTT +DNQ  V   V++GER    +N         G     
Sbjct: 412 VFGVVCPRNTPIPTIKKRTFTTVADNQTTVTFPVYQGERVNCAENEPLGEFQLTGIPPMP 471

Query: 298 DGVPQIEVTFDIDANGILNVSAIEKSTNKENKITITNDKGRLSKEEIERMVNEAEKYRNE 477
            G  ++E TF++DANGIL V+A+EK+T +   I ITN  G LS  +I+ M+  A+K++ +
Sbjct: 472 RGQAELEATFELDANGILKVTAVEKTTGRSAHIEITNSVGHLSSTKIQEMIENADKFKQQ 531

Query: 478 DDKQKETIQAR 510
           D    + ++A+
Sbjct: 532 DKDFAKKLEAK 542


>SPCC1235.05c |fft2||fun thirty related protein
            Fft2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1284

 Score = 33.1 bits (72), Expect = 0.033
 Identities = 29/92 (31%), Positives = 44/92 (47%), Gaps = 4/92 (4%)
 Frame = +1

Query: 178  TTYSDNQPGVLIQVFEGERAMTKDNNCSVNSS*PGSHRRXDGVPQIEVTFDIDANGILNV 357
            T   DN+   L +  +GE   T D+N +V SS   +    + V    +  D +A+ +  V
Sbjct: 1164 TVEGDNKE--LRETMKGENVQT-DSNAAVPSSKSSTEEPNESVLSGHLDLDTEASPV--V 1218

Query: 358  SAIEKSTNKENKIT----ITNDKGRLSKEEIE 441
            S IEK+T  +  +T      N  G+L K EIE
Sbjct: 1219 STIEKTTKGDVSVTEEQQSANIDGQLEKPEIE 1250


>SPBC16C6.05 |||translation initiation factor |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 190

 Score = 30.3 bits (65), Expect = 0.23
 Identities = 16/53 (30%), Positives = 27/53 (50%)
 Frame = +1

Query: 334 DANGILNVSAIEKSTNKENKITITNDKGRLSKEEIERMVNEAEKYRNEDDKQK 492
           D    LNVS  + S  +E    +T ++ R+ +EE +RM ++      E  K+K
Sbjct: 58  DLENTLNVSGTKDSNAEEQPAKLTKEEKRVEREEAKRMASKVLIKTIERTKRK 110


>SPCC663.03 |pmd1||leptomycin efflux transporter
           Pmd1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1362

 Score = 29.1 bits (62), Expect = 0.53
 Identities = 14/24 (58%), Positives = 16/24 (66%)
 Frame = +1

Query: 412 KGRLSKEEIERMVNEAEKYRNEDD 483
           KG LSKEE+ER V +A K  N  D
Sbjct: 525 KGTLSKEELERRVYDAAKLANAYD 548


>SPBC16E9.13 |ksp1|ppk20|serine/threonine protein kinase Ksp1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 474

 Score = 27.9 bits (59), Expect = 1.2
 Identities = 15/44 (34%), Positives = 22/44 (50%)
 Frame = +1

Query: 349 LNVSAIEKSTNKENKITITNDKGRLSKEEIERMVNEAEKYRNED 480
           L VS  ++S  K  +  +   K     EE+E  VNE E++R  D
Sbjct: 257 LCVSPEKRSLAKTREAVLAVTKWTTDDEELESFVNEEEEFRASD 300


>SPBC3B9.02c |cwf28||splicing factor Cwf28|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 381

 Score = 27.5 bits (58), Expect = 1.6
 Identities = 13/36 (36%), Positives = 17/36 (47%), Gaps = 1/36 (2%)
 Frame = +3

Query: 342 RYPQRFRYREVHQQGEQDHHYQRQRSS-LQGRDRAY 446
           R P R +YRE H +  + H   R      QGR  +Y
Sbjct: 320 RDPDRTKYREYHSERRKQHRTDRYSDDYYQGRSYSY 355


>SPBC1734.01c ||SPBC337.17c|RNA-binding protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 682

 Score = 26.6 bits (56), Expect = 2.8
 Identities = 13/28 (46%), Positives = 16/28 (57%)
 Frame = +1

Query: 379 NKENKITITNDKGRLSKEEIERMVNEAE 462
           NK+ K T   D    SKEE+E +V E E
Sbjct: 529 NKKGKHTQIEDPTAASKEELENLVREDE 556


>SPAC20G4.08 ||SPAC4F10.01|sequence orphan|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 1076

 Score = 26.6 bits (56), Expect = 2.8
 Identities = 23/84 (27%), Positives = 38/84 (45%), Gaps = 1/84 (1%)
 Frame = +1

Query: 136 KRNTTIPTKQNQTFTTYSDNQPGVLIQVFE-GERAMTKDNNCSVNSS*PGSHRRXDGVPQ 312
           + +T  P+  N  F+T      G   +  + GER  TKD + SV S    S    D +P 
Sbjct: 666 RHSTASPSTVNSGFSTPRSQATGFSKKKKDKGERFETKDKSSSVLSPSSYSASTFDAIPM 725

Query: 313 IEVTFDIDANGILNVSAIEKSTNK 384
             +  +I       ++++EKS +K
Sbjct: 726 DSIVSNI-------LASLEKSVHK 742


>SPAC688.11 |end4|sla2|Huntingtin-interacting protein
            homolog|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1092

 Score = 25.8 bits (54), Expect = 5.0
 Identities = 15/49 (30%), Positives = 25/49 (51%)
 Frame = -3

Query: 380  LVDFSIAETLRIPLASMSKVTSI*GTPSXRRWDPGQLEFTEQLLSLVIA 234
            LVD  +AET +    ++ ++ +I   P      P +L+  + LLS  IA
Sbjct: 859  LVDNQLAETAQAIQQAILRLQNIAAKPKDDSLSPSELQVHDSLLSASIA 907


>SPAC1556.07 |pmm1||phosphomannomutase Pmm1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 257

 Score = 25.4 bits (53), Expect = 6.6
 Identities = 15/55 (27%), Positives = 27/55 (49%), Gaps = 1/55 (1%)
 Frame = +1

Query: 304 VPQIEVTFDIDANGILNVSAIEKSTNKENKITITN-DKGRLSKEEIERMVNEAEK 465
           +P    TF    NG++N+S + ++ N E +      DKGR  +  +  ++ E  K
Sbjct: 122 IPVKRGTFIEFRNGMINISPVGRNANTEERNEFERFDKGRKIRATMVDVLREKFK 176


>SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1067

 Score = 25.4 bits (53), Expect = 6.6
 Identities = 12/47 (25%), Positives = 26/47 (55%)
 Frame = +1

Query: 355 VSAIEKSTNKENKITITNDKGRLSKEEIERMVNEAEKYRNEDDKQKE 495
           V A+ KS +++    ++   G  + EEI ++++  E    E+D++ E
Sbjct: 597 VPALFKSIDQDESDILSERIGAPTAEEISQLLDSVETNEEENDEELE 643


>SPAC22F8.10c |sap145||U2 snRNP-associated protein Sap145
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 601

 Score = 25.4 bits (53), Expect = 6.6
 Identities = 14/43 (32%), Positives = 23/43 (53%)
 Frame = +1

Query: 370 KSTNKENKITITNDKGRLSKEEIERMVNEAEKYRNEDDKQKET 498
           K  NK+++  +  +K +L +E+       AEK  N DDK + T
Sbjct: 39  KQKNKKSRNQVRREKKKLLREKTNSGAKLAEK--NSDDKDQLT 79


>SPAC2F7.03c |pom1||DYRK family protein kinase
           Pom1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1087

 Score = 25.4 bits (53), Expect = 6.6
 Identities = 14/47 (29%), Positives = 23/47 (48%)
 Frame = +1

Query: 340 NGILNVSAIEKSTNKENKITITNDKGRLSKEEIERMVNEAEKYRNED 480
           N + N S  +KS+NKE + +      RLS       + +  K ++ED
Sbjct: 409 NTVQNESKSQKSSNKEGRSSRGGFFSRLSFSRSSSRMKKGSKAKHED 455


>SPBC16G5.16 |||transcription factor zf-fungal binuclear cluster
           type |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 827

 Score = 25.4 bits (53), Expect = 6.6
 Identities = 12/28 (42%), Positives = 14/28 (50%)
 Frame = +2

Query: 143 TLPSPLNRIRHSPPTLITNPEYSSKYLR 226
           T   P  R+RH+P   I   E   KYLR
Sbjct: 43  TYGQPFKRLRHAPEKYIEFLELRLKYLR 70


>SPCC126.03 |pus1|SPCC126.03, SPCC126.03|tRNA pseudouridylate
           synthase Lsp1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 534

 Score = 25.4 bits (53), Expect = 6.6
 Identities = 14/32 (43%), Positives = 19/32 (59%)
 Frame = +3

Query: 489 KGDHPGQNALESYCFSMKSTMEDEKLKEKISD 584
           KG H   N +     S+K  MEDEKL EK+++
Sbjct: 104 KGVHAAGNVI-----SLKLIMEDEKLIEKVNE 130


>SPBC2F12.05c |||sterol binding ankyrin repeat
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1310

 Score = 25.0 bits (52), Expect = 8.7
 Identities = 17/62 (27%), Positives = 30/62 (48%)
 Frame = +1

Query: 139 RNTTIPTKQNQTFTTYSDNQPGVLIQVFEGERAMTKDNNCSVNSS*PGSHRRXDGVPQIE 318
           R TT+ T Q + FT   DN   +  +    E+ + K++  S  S+   +H R + +P  +
Sbjct: 627 RKTTL-TDQTEEFTERRDNNGSIPSKQPSDEQHLGKESLPSQQST-VSNHHRKESIPSKQ 684

Query: 319 VT 324
            T
Sbjct: 685 PT 686


>SPAC4H3.10c |pyk1||pyruvate kinase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 509

 Score = 25.0 bits (52), Expect = 8.7
 Identities = 11/32 (34%), Positives = 22/32 (68%)
 Frame = +1

Query: 340 NGILNVSAIEKSTNKENKITITNDKGRLSKEE 435
           +GIL+ + IEK  +K  K+ + N+ G++S ++
Sbjct: 154 DGILSFTVIEKVDDKNLKVRV-NNNGKISSKK 184


>SPBC1652.01 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 386

 Score = 25.0 bits (52), Expect = 8.7
 Identities = 14/58 (24%), Positives = 26/58 (44%)
 Frame = +1

Query: 97  VLRLLDXVMTTLIKRNTTIPTKQNQTFTTYSDNQPGVLIQVFEGERAMTKDNNCSVNS 270
           + RLL  +   L   N   PT+Q +  T   +++ G+  +     R + +D+    NS
Sbjct: 128 IRRLLHHLSLMLPSFNELTPTQQRRLLTRALESKKGIQFEKIGWGRWVLRDSTIPANS 185


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,222,607
Number of Sequences: 5004
Number of extensions: 40738
Number of successful extensions: 184
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 161
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 173
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 269634532
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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