BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00638 (739 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g32410.1 68415.m03960 auxin-resistance protein, putative stro... 74 9e-14 At1g05180.1 68414.m00522 auxin-resistance protein AXR1 (AXR1) id... 74 1e-13 At1g05180.2 68414.m00521 auxin-resistance protein AXR1 (AXR1) id... 51 7e-07 At5g50680.1 68418.m06280 SUMO activating enzyme 1b (SAE1b) ident... 36 0.028 At5g50580.2 68418.m06266 SUMO activating enzyme, putative nearly... 36 0.028 At5g50580.1 68418.m06265 SUMO activating enzyme, putative nearly... 36 0.028 At5g06460.1 68418.m00724 ubiquitin activating enzyme 2 (UBA2) E1... 35 0.065 At2g30110.1 68415.m03664 ubiquitin activating enzyme 1 (UBA1) E1... 35 0.065 At4g24940.1 68417.m03572 SUMO activating enzyme 1a (SAE1a) ident... 32 0.46 At3g53980.2 68416.m05965 protease inhibitor/seed storage/lipid t... 31 1.1 At3g53980.1 68416.m05964 protease inhibitor/seed storage/lipid t... 31 1.1 At1g77240.1 68414.m08996 AMP-binding protein, putative strong si... 30 1.8 At5g44960.1 68418.m05514 F-box family protein contains F-box dom... 29 2.4 At1g75960.1 68414.m08822 AMP-binding protein, putative similar t... 29 4.3 At5g05960.1 68418.m00659 protease inhibitor/seed storage/lipid t... 28 5.6 At1g30890.1 68414.m03779 integral membrane HRF1 family protein c... 28 7.4 At1g21530.1 68414.m02693 AMP-binding protein, putative strong si... 28 7.4 At1g10410.1 68414.m01173 expressed protein similar to ESTs gb|N9... 28 7.4 At1g59650.1 68414.m06709 expressed protein ; supporting cDNA gi|... 27 9.8 At1g21540.1 68414.m02694 AMP-binding protein, putative strong si... 27 9.8 At1g09090.2 68414.m01015 respiratory burst oxidase protein B (Rb... 27 9.8 At1g09090.1 68414.m01014 respiratory burst oxidase protein B (Rb... 27 9.8 >At2g32410.1 68415.m03960 auxin-resistance protein, putative strong similarity to SP|P42744 Auxin-resistance protein AXR1 {Arabidopsis thaliana}; contains Pfam profile PF00899: ThiF family Length = 523 Score = 74.1 bits (174), Expect = 9e-14 Identities = 35/88 (39%), Positives = 55/88 (62%) Frame = +1 Query: 247 IEMIVLPGIGAITIVDDCTVSDEDIGSNFFLEMSSKGLNRGSEALRLVLELNPAVQGHAV 426 ++ +V+ GIG+ITIVD V D+G+NF ++ S G +R + ELN +V+ + V Sbjct: 41 LKNLVIGGIGSITIVDGSKVEIGDLGNNFMVDAKSVGQSRAKTVCGFLQELNDSVKANFV 100 Query: 427 QEPPDQILDENPDFFKSFSLVIATTISE 510 +E PD ++ +P FF F+LVIAT + E Sbjct: 101 EENPDTLISTDPSFFSQFTLVIATQLVE 128 Score = 55.6 bits (128), Expect = 3e-08 Identities = 25/65 (38%), Positives = 38/65 (58%) Frame = +3 Query: 543 DINVPFILCQSVGFLGSFRIQIREHAVTETHPDNEQTDLRLDEPFMSLLEHLESIDIDAL 722 + NV +L +S G G RI ++EH ET PD+ DLRL+ P+ L ++ESID++ Sbjct: 140 EANVMLVLARSYGLTGFVRISVKEHTAIETKPDHSLDDLRLNSPWPELKSYVESIDLNVE 199 Query: 723 DLKDH 737 + H Sbjct: 200 EPAAH 204 Score = 52.4 bits (120), Expect = 3e-07 Identities = 22/36 (61%), Positives = 28/36 (77%) Frame = +2 Query: 146 QYDRQLRLWGDHGQSALESGHICLINATALGTEILK 253 +YDRQLR+WG+ GQSALE+ ICL+N G+E LK Sbjct: 7 KYDRQLRIWGELGQSALETASICLLNCGPTGSEALK 42 >At1g05180.1 68414.m00522 auxin-resistance protein AXR1 (AXR1) identical to SP|P42744 Auxin-resistance protein AXR1 {Arabidopsis thaliana}; contains Pfam profile PF00899: ThiF family; identical to cDNA ubiquitin activating enzyme E1-related protein (AXR1) GI:304103 Length = 540 Score = 73.7 bits (173), Expect = 1e-13 Identities = 33/88 (37%), Positives = 53/88 (60%) Frame = +1 Query: 247 IEMIVLPGIGAITIVDDCTVSDEDIGSNFFLEMSSKGLNRGSEALRLVLELNPAVQGHAV 426 ++ +VL G+G+IT+VD V D+G+NF ++ S G ++ + ELN +V + Sbjct: 57 LKNLVLGGVGSITVVDGSKVQFGDLGNNFMVDAKSVGQSKAKSVCAFLQELNDSVNAKFI 116 Query: 427 QEPPDQILDENPDFFKSFSLVIATTISE 510 +E PD ++ NP FF F+LVIAT + E Sbjct: 117 EENPDTLITTNPSFFSQFTLVIATQLVE 144 Score = 51.2 bits (117), Expect = 7e-07 Identities = 22/58 (37%), Positives = 36/58 (62%) Frame = +3 Query: 543 DINVPFILCQSVGFLGSFRIQIREHAVTETHPDNEQTDLRLDEPFMSLLEHLESIDID 716 D NV +L +S G G RI ++EH + ++ PD+ DLRL+ P+ L +E+ID++ Sbjct: 156 DANVKLVLVRSYGLAGFVRISVKEHPIIDSKPDHFLDDLRLNNPWPELKSFVETIDLN 213 Score = 50.8 bits (116), Expect = 9e-07 Identities = 21/36 (58%), Positives = 27/36 (75%) Frame = +2 Query: 146 QYDRQLRLWGDHGQSALESGHICLINATALGTEILK 253 +YDRQLR+WG+ GQ+ALE ICL+N G+E LK Sbjct: 23 KYDRQLRIWGEVGQAALEEASICLLNCGPTGSEALK 58 >At1g05180.2 68414.m00521 auxin-resistance protein AXR1 (AXR1) identical to SP|P42744 Auxin-resistance protein AXR1 {Arabidopsis thaliana}; contains Pfam profile PF00899: ThiF family; identical to cDNA ubiquitin activating enzyme E1-related protein (AXR1) GI:304103 Length = 436 Score = 51.2 bits (117), Expect = 7e-07 Identities = 22/58 (37%), Positives = 36/58 (62%) Frame = +3 Query: 543 DINVPFILCQSVGFLGSFRIQIREHAVTETHPDNEQTDLRLDEPFMSLLEHLESIDID 716 D NV +L +S G G RI ++EH + ++ PD+ DLRL+ P+ L +E+ID++ Sbjct: 52 DANVKLVLVRSYGLAGFVRISVKEHPIIDSKPDHFLDDLRLNNPWPELKSFVETIDLN 109 >At5g50680.1 68418.m06280 SUMO activating enzyme 1b (SAE1b) identical to SUMO activating enzyme 1b [Arabidopsis thaliana] GI:22652852; nearly identical to At5g50580; contains Pfam profile PF00899: ThiF family Length = 320 Score = 35.9 bits (79), Expect = 0.028 Identities = 28/98 (28%), Positives = 44/98 (44%), Gaps = 3/98 (3%) Frame = +1 Query: 256 IVLPGIGAITIVDDCTVSDEDIGSNFFL---EMSSKGLNRGSEALRLVLELNPAVQGHAV 426 IVL G+G++T++DD V+ E +NF + E + G + + NP V H Sbjct: 50 IVLAGVGSVTLLDDRLVTTEVFNANFLILPDENAYVGKTVAEICCDSLKDFNPMV--HVS 107 Query: 427 QEPPDQILDENPDFFKSFSLVIATTISEK*RKVYLNTC 540 E D + DFF+ F +V+ S +K C Sbjct: 108 IEKGD-LSTLGVDFFEKFDVVVIGYSSRATKKAVNEKC 144 Score = 29.5 bits (63), Expect = 2.4 Identities = 12/35 (34%), Positives = 16/35 (45%) Frame = +2 Query: 149 YDRQLRLWGDHGQSALESGHICLINATALGTEILK 253 YDRQ+R+WG Q L H+ + E K Sbjct: 14 YDRQIRVWGAGAQRRLSKSHVLVSGIKGTVAEFCK 48 >At5g50580.2 68418.m06266 SUMO activating enzyme, putative nearly identical to SUMO activating enzyme 1b [Arabidopsis thaliana] GI:22652852; nearly identical to At5g50680; contains Pfam profile PF00899: ThiF family Length = 320 Score = 35.9 bits (79), Expect = 0.028 Identities = 28/98 (28%), Positives = 44/98 (44%), Gaps = 3/98 (3%) Frame = +1 Query: 256 IVLPGIGAITIVDDCTVSDEDIGSNFFL---EMSSKGLNRGSEALRLVLELNPAVQGHAV 426 IVL G+G++T++DD V+ E +NF + E + G + + NP V H Sbjct: 50 IVLAGVGSVTLLDDRLVTTEVFNANFLILPDENAYVGKTVAEICCDSLKDFNPMV--HVS 107 Query: 427 QEPPDQILDENPDFFKSFSLVIATTISEK*RKVYLNTC 540 E D + DFF+ F +V+ S +K C Sbjct: 108 IEKGD-LSTLGVDFFEKFDVVVIGYSSRATKKAVNEKC 144 Score = 29.5 bits (63), Expect = 2.4 Identities = 12/35 (34%), Positives = 16/35 (45%) Frame = +2 Query: 149 YDRQLRLWGDHGQSALESGHICLINATALGTEILK 253 YDRQ+R+WG Q L H+ + E K Sbjct: 14 YDRQIRVWGAGAQRRLSKSHVLVSGIKGTVAEFCK 48 >At5g50580.1 68418.m06265 SUMO activating enzyme, putative nearly identical to SUMO activating enzyme 1b [Arabidopsis thaliana] GI:22652852; nearly identical to At5g50680; contains Pfam profile PF00899: ThiF family Length = 318 Score = 35.9 bits (79), Expect = 0.028 Identities = 28/98 (28%), Positives = 44/98 (44%), Gaps = 3/98 (3%) Frame = +1 Query: 256 IVLPGIGAITIVDDCTVSDEDIGSNFFL---EMSSKGLNRGSEALRLVLELNPAVQGHAV 426 IVL G+G++T++DD V+ E +NF + E + G + + NP V H Sbjct: 50 IVLAGVGSVTLLDDRLVTTEVFNANFLILPDENAYVGKTVAEICCDSLKDFNPMV--HVS 107 Query: 427 QEPPDQILDENPDFFKSFSLVIATTISEK*RKVYLNTC 540 E D + DFF+ F +V+ S +K C Sbjct: 108 IEKGD-LSTLGVDFFEKFDVVVIGYSSRATKKAVNEKC 144 Score = 29.5 bits (63), Expect = 2.4 Identities = 12/35 (34%), Positives = 16/35 (45%) Frame = +2 Query: 149 YDRQLRLWGDHGQSALESGHICLINATALGTEILK 253 YDRQ+R+WG Q L H+ + E K Sbjct: 14 YDRQIRVWGAGAQRRLSKSHVLVSGIKGTVAEFCK 48 >At5g06460.1 68418.m00724 ubiquitin activating enzyme 2 (UBA2) E1; identical to gi:1703477 Length = 1077 Score = 34.7 bits (76), Expect = 0.065 Identities = 18/52 (34%), Positives = 28/52 (53%) Frame = +1 Query: 256 IVLPGIGAITIVDDCTVSDEDIGSNFFLEMSSKGLNRGSEALRLVLELNPAV 411 I+L G+ ++T+ D+ V D+ SNF G NR ++ + ELN AV Sbjct: 111 IILAGVKSVTLHDENVVELWDLSSNFVFTEEDIGKNRALASVHKLQELNNAV 162 Score = 33.9 bits (74), Expect = 0.11 Identities = 15/38 (39%), Positives = 22/38 (57%) Frame = +2 Query: 140 NKQYDRQLRLWGDHGQSALESGHICLINATALGTEILK 253 N +YD Q+ ++G Q LE + ++ A ALG E LK Sbjct: 469 NSRYDAQISVFGSTLQKKLEDARVFVVGAGALGCEFLK 506 >At2g30110.1 68415.m03664 ubiquitin activating enzyme 1 (UBA1) E1; identical to GB:U80808 Length = 1080 Score = 34.7 bits (76), Expect = 0.065 Identities = 16/52 (30%), Positives = 29/52 (55%) Frame = +1 Query: 256 IVLPGIGAITIVDDCTVSDEDIGSNFFLEMSSKGLNRGSEALRLVLELNPAV 411 ++L G+ ++T+ D+ V D+ SNF G NR +++ + +LN AV Sbjct: 114 LILAGVKSVTLHDERVVELWDLSSNFVFSEDDVGKNRADASVQKLQDLNNAV 165 Score = 31.5 bits (68), Expect = 0.60 Identities = 14/38 (36%), Positives = 21/38 (55%) Frame = +2 Query: 140 NKQYDRQLRLWGDHGQSALESGHICLINATALGTEILK 253 N +YD Q+ ++G Q LE + + + ALG E LK Sbjct: 472 NSRYDAQISVFGAKFQKKLEDAKVFTVGSGALGCEFLK 509 >At4g24940.1 68417.m03572 SUMO activating enzyme 1a (SAE1a) identical to SUMO activating enzyme 1a [Arabidopsis thaliana] GI:22652850; contains Pfam profile PF00899: ThiF family Length = 322 Score = 31.9 bits (69), Expect = 0.46 Identities = 23/82 (28%), Positives = 42/82 (51%), Gaps = 3/82 (3%) Frame = +1 Query: 256 IVLPGIGAITIVDDCTVSDEDIGSNFFL---EMSSKGLNRGSEALRLVLELNPAVQGHAV 426 IVL G+G++T++DD + E + +NF + E G + + NP V+ +V Sbjct: 50 IVLAGVGSVTLMDDRLANMEALNANFLIPPDENVYSGKTVAEICSDSLKDFNPMVR-VSV 108 Query: 427 QEPPDQILDENPDFFKSFSLVI 492 ++ +L DFF+ F +V+ Sbjct: 109 EKGDLSML--GTDFFEQFDVVV 128 Score = 30.7 bits (66), Expect = 1.1 Identities = 13/35 (37%), Positives = 17/35 (48%) Frame = +2 Query: 149 YDRQLRLWGDHGQSALESGHICLINATALGTEILK 253 YDRQ+R+WG + Q L HI + E K Sbjct: 14 YDRQIRVWGANAQRRLTKAHILVSGIKGTVAEFCK 48 >At3g53980.2 68416.m05965 protease inhibitor/seed storage/lipid transfer protein (LTP) family protein contains Pfam protease inhibitor/seed storage/LTP family domain PF00234 Length = 114 Score = 30.7 bits (66), Expect = 1.1 Identities = 11/22 (50%), Positives = 13/22 (59%) Frame = +2 Query: 506 VKSNAKFISTPVGYKCSFYTLP 571 + F + PVGYKC YTLP Sbjct: 93 IPKRCNFANRPVGYKCGAYTLP 114 >At3g53980.1 68416.m05964 protease inhibitor/seed storage/lipid transfer protein (LTP) family protein contains Pfam protease inhibitor/seed storage/LTP family domain PF00234 Length = 114 Score = 30.7 bits (66), Expect = 1.1 Identities = 11/22 (50%), Positives = 13/22 (59%) Frame = +2 Query: 506 VKSNAKFISTPVGYKCSFYTLP 571 + F + PVGYKC YTLP Sbjct: 93 IPKRCNFANRPVGYKCGAYTLP 114 >At1g77240.1 68414.m08996 AMP-binding protein, putative strong similarity to AMP-binding protein GI:1903034 from [Brassica napus]; contains Pfam AMP-binding domain PF00501 Length = 545 Score = 29.9 bits (64), Expect = 1.8 Identities = 17/41 (41%), Positives = 21/41 (51%) Frame = +1 Query: 355 GLNRGSEALRLVLELNPAVQGHAVQEPPDQILDENPDFFKS 477 G N S L VL NPA++ AV PD++ E P F S Sbjct: 448 GENISSTELEAVLYTNPAIKEAAVVAKPDKMWGETPCAFVS 488 >At5g44960.1 68418.m05514 F-box family protein contains F-box domain Pfam:PF00646 Length = 416 Score = 29.5 bits (63), Expect = 2.4 Identities = 17/60 (28%), Positives = 31/60 (51%) Frame = +1 Query: 424 VQEPPDQILDENPDFFKSFSLVIATTISEK*RKVYLNTCGI*MFLLYFANQLDS*GLLEF 603 + E PD +L + ++ V + S++ R ++LN G+ +F L F N GL++F Sbjct: 7 INELPDSLLTQILLDLRTKDSVKTSVSSKRWRNLWLNVPGLDLFSLQFTNPHHEEGLIKF 66 >At1g75960.1 68414.m08822 AMP-binding protein, putative similar to AMP-binding protein GI:1903034 from [Brassica napus]; contains Pfam profile: PF00501 AMP-binding enzyme; identical to cDNA adenosine monophosphate binding protein 8 AMPBP8 (AMPBP8) GI:20799724 Length = 544 Score = 28.7 bits (61), Expect = 4.3 Identities = 18/43 (41%), Positives = 20/43 (46%) Frame = +1 Query: 349 SKGLNRGSEALRLVLELNPAVQGHAVQEPPDQILDENPDFFKS 477 S G N S + VL NPAV AV PD+ E P F S Sbjct: 445 SGGENVSSVEVEAVLYTNPAVNEAAVVARPDEFWGETPCAFVS 487 >At5g05960.1 68418.m00659 protease inhibitor/seed storage/lipid transfer protein (LTP) family protein contains Pfam protease inhibitor/seed storage/LTP family domain PF00234 Length = 116 Score = 28.3 bits (60), Expect = 5.6 Identities = 10/22 (45%), Positives = 12/22 (54%) Frame = +2 Query: 506 VKSNAKFISTPVGYKCSFYTLP 571 + + PVGYKC YTLP Sbjct: 95 IPKRCNIANRPVGYKCGAYTLP 116 >At1g30890.1 68414.m03779 integral membrane HRF1 family protein contains Pfam domain PF03878: Hrf1 family Length = 269 Score = 27.9 bits (59), Expect = 7.4 Identities = 10/17 (58%), Positives = 12/17 (70%) Frame = -2 Query: 462 WVLIKYLVGWFLDCMTL 412 W+ +K LVGWFL M L Sbjct: 149 WLFVKGLVGWFLQVMLL 165 >At1g21530.1 68414.m02693 AMP-binding protein, putative strong similarity to AMP-binding protein GI:1903034 from [Brassica napus]; contains Pfam AMP-binding domain PF00501; identical to cDNA adenosine monophosphate binding protein 10 AMPBP10 (AMPBP10) GI:20799728 Length = 547 Score = 27.9 bits (59), Expect = 7.4 Identities = 16/41 (39%), Positives = 20/41 (48%) Frame = +1 Query: 355 GLNRGSEALRLVLELNPAVQGHAVQEPPDQILDENPDFFKS 477 G N S + VL NP V+ AV PD++ E P F S Sbjct: 445 GENISSAEIETVLYTNPVVKEAAVVAKPDKMWGETPCAFVS 485 >At1g10410.1 68414.m01173 expressed protein similar to ESTs gb|N96021 and gb|N96863 Length = 485 Score = 27.9 bits (59), Expect = 7.4 Identities = 16/46 (34%), Positives = 27/46 (58%), Gaps = 1/46 (2%) Frame = +1 Query: 316 DIGSNFFLEMSSKGLNRGSEALRL-VLELNPAVQGHAVQEPPDQIL 450 DI + F +S KG + L++ VL++ +QG+ +E P+QIL Sbjct: 419 DIDMHRFGYISRKGFETFIDRLKICVLDVGLTIQGNKPEELPEQIL 464 >At1g59650.1 68414.m06709 expressed protein ; supporting cDNA gi|6520232|dbj|AB028233.1| Length = 492 Score = 27.5 bits (58), Expect = 9.8 Identities = 16/46 (34%), Positives = 26/46 (56%), Gaps = 1/46 (2%) Frame = +1 Query: 316 DIGSNFFLEMSSKGLNRGSEALR-LVLELNPAVQGHAVQEPPDQIL 450 DI + F +S KG + L+ VL++ +QG+ +E P+QIL Sbjct: 424 DIDMHRFSYISRKGFEAFLDRLKNCVLDVGLTIQGNKPEELPEQIL 469 >At1g21540.1 68414.m02694 AMP-binding protein, putative strong similarity to AMP-binding protein GI:1903034 from [Brassica napus]; contains Pfam AMP-binding domain PF00501; identical to cDNA adenosine monophosphate binding protein 9 AMPBP9 (AMPBP9) GI:20799726 Length = 550 Score = 27.5 bits (58), Expect = 9.8 Identities = 16/41 (39%), Positives = 20/41 (48%) Frame = +1 Query: 355 GLNRGSEALRLVLELNPAVQGHAVQEPPDQILDENPDFFKS 477 G N S + VL NP V+ AV PD++ E P F S Sbjct: 450 GENISSAEVETVLYTNPVVKEAAVVAKPDKMWGETPCAFVS 490 >At1g09090.2 68414.m01015 respiratory burst oxidase protein B (RbohB) / NADPH oxidase identical to respiratory burst oxidase protein B from Arabidopsis thaliana [gi:3242783] Length = 843 Score = 27.5 bits (58), Expect = 9.8 Identities = 18/57 (31%), Positives = 27/57 (47%) Frame = -2 Query: 573 IGKV*KEHLYPTGVEINFALLFTYCSSYH*TE*FKKVWVLIKYLVGWFLDCMTLYCW 403 + K+ + L PT + N F SY E +K++WVL W C+TL+ W Sbjct: 263 LNKMLSQKLIPTK-DRNPVKRFAMNISYFFLENWKRIWVLTL----WISICITLFTW 314 >At1g09090.1 68414.m01014 respiratory burst oxidase protein B (RbohB) / NADPH oxidase identical to respiratory burst oxidase protein B from Arabidopsis thaliana [gi:3242783] Length = 622 Score = 27.5 bits (58), Expect = 9.8 Identities = 18/57 (31%), Positives = 27/57 (47%) Frame = -2 Query: 573 IGKV*KEHLYPTGVEINFALLFTYCSSYH*TE*FKKVWVLIKYLVGWFLDCMTLYCW 403 + K+ + L PT + N F SY E +K++WVL W C+TL+ W Sbjct: 263 LNKMLSQKLIPTK-DRNPVKRFAMNISYFFLENWKRIWVLTL----WISICITLFTW 314 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,920,229 Number of Sequences: 28952 Number of extensions: 261235 Number of successful extensions: 615 Number of sequences better than 10.0: 22 Number of HSP's better than 10.0 without gapping: 594 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 615 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1624036432 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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