Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00629
(669 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g31300.1 68415.m03821 transducin family protein / WD-40 repea... 73 1e-13
At2g30910.2 68415.m03768 transducin family protein / WD-40 repea... 73 1e-13
At2g30910.1 68415.m03767 transducin family protein / WD-40 repea... 73 1e-13
At3g10790.1 68416.m01299 F-box family protein contains F-box dom... 30 1.6
At4g34460.2 68417.m04898 guanine nucleotide-binding protein beta... 29 2.1
At4g32551.1 68417.m04633 WD-40 repeat family protein (LEUNIG) co... 28 6.5
At3g01780.1 68416.m00118 expressed protein est hit, 28 6.5
At2g01330.1 68415.m00050 transducin family protein / WD-40 repea... 28 6.5
At4g14220.1 68417.m02194 zinc finger (C3HC4-type RING finger) fa... 27 8.5
At3g01340.1 68416.m00051 protein transport protein SEC13 family ... 27 8.5
At2g30050.1 68415.m03654 transducin family protein / WD-40 repea... 27 8.5
>At2g31300.1 68415.m03821 transducin family protein / WD-40 repeat
family protein contains 6 WD-40 repeats (PF00400);
identical to putative ARP2/3 protein complex subunit p41
(GI:4432825)[Arabidopsis thaliana]; similar to ARP2/3
complex 41 kDa subunit (P41-ARC) (Actin-related protein
2/3 complex subunit 1B) (SP:Q9WV32) [Mus musculus]
Length = 378
Score = 73.3 bits (172), Expect = 1e-13
Identities = 29/44 (65%), Positives = 37/44 (84%)
Frame = +3
Query: 507 DKFAVGSGARLISICYFEKENNWWVSKHIKKPIRSTVTSIDWHP 638
+KFAVGSGA+ + ICY+E+ENNWWVSK I+K S+VTS+ WHP
Sbjct: 112 NKFAVGSGAKTVCICYYEQENNWWVSKLIRKRHESSVTSVAWHP 155
Score = 58.4 bits (135), Expect = 4e-09
Identities = 37/106 (34%), Positives = 51/106 (48%), Gaps = 2/106 (1%)
Frame = +1
Query: 214 ITCHAWNKDRSQIA--SHQTIMKFTYTKGRK*LEANKQPCGTRP*SDGD*LGSQHQPYCY 387
ITCHAW+ D S +A + T + + + E G S+
Sbjct: 13 ITCHAWSPDLSMVALCPNNTEVHIYKSLSQDHWERLHVLQKHDQIVSGIDWSSKSNKIVT 72
Query: 388 VFVDRNAYVWTQGDDGKWATTLVLLRINRAATCVKWSPMETSLLSG 525
V DRN+YVW+ + +W TLV+LR+NRAA CV+WSP E G
Sbjct: 73 VSHDRNSYVWSL-EGAEWVPTLVILRLNRAALCVQWSPKENKFAVG 117
Score = 35.5 bits (78), Expect = 0.032
Identities = 17/40 (42%), Positives = 25/40 (62%), Gaps = 2/40 (5%)
Frame = +2
Query: 281 HIQKE--GNDWKQTNNLVEHDLRVMGIDWAPNTNRIVTCS 394
HI K + W++ + L +HD V GIDW+ +N+IVT S
Sbjct: 35 HIYKSLSQDHWERLHVLQKHDQIVSGIDWSSKSNKIVTVS 74
>At2g30910.2 68415.m03768 transducin family protein / WD-40 repeat
family protein contains 6 WD-40 repeats (PF00400) (1
weak); similar to ARP2/3 complex 41 kDa subunit
(P41-ARC) (Actin-related protein 2/3 complex subunit 1B)
(SP:O88656) [Rattus norvegicus]
Length = 378
Score = 73.3 bits (172), Expect = 1e-13
Identities = 29/44 (65%), Positives = 37/44 (84%)
Frame = +3
Query: 507 DKFAVGSGARLISICYFEKENNWWVSKHIKKPIRSTVTSIDWHP 638
+KFAVGSGA+ + ICY+E+ENNWWVSK I+K S+VTS+ WHP
Sbjct: 112 NKFAVGSGAKTVCICYYEQENNWWVSKLIRKRHESSVTSVAWHP 155
Score = 58.4 bits (135), Expect = 4e-09
Identities = 37/106 (34%), Positives = 51/106 (48%), Gaps = 2/106 (1%)
Frame = +1
Query: 214 ITCHAWNKDRSQIA--SHQTIMKFTYTKGRK*LEANKQPCGTRP*SDGD*LGSQHQPYCY 387
ITCHAW+ D S +A + T + + + E G S+
Sbjct: 13 ITCHAWSPDLSMVALCPNNTEVHIYKSLSQDHWERLHVLQKHDQIVSGIDWSSKSNKIVT 72
Query: 388 VFVDRNAYVWTQGDDGKWATTLVLLRINRAATCVKWSPMETSLLSG 525
V DRN+YVW+ + +W TLV+LR+NRAA CV+WSP E G
Sbjct: 73 VSHDRNSYVWSL-EGAEWVPTLVILRLNRAALCVQWSPKENKFAVG 117
Score = 35.5 bits (78), Expect = 0.032
Identities = 17/40 (42%), Positives = 25/40 (62%), Gaps = 2/40 (5%)
Frame = +2
Query: 281 HIQKE--GNDWKQTNNLVEHDLRVMGIDWAPNTNRIVTCS 394
HI K + W++ + L +HD V GIDW+ +N+IVT S
Sbjct: 35 HIYKSLSQDHWERLHVLQKHDQIVSGIDWSSKSNKIVTVS 74
>At2g30910.1 68415.m03767 transducin family protein / WD-40 repeat
family protein contains 6 WD-40 repeats (PF00400) (1
weak); similar to ARP2/3 complex 41 kDa subunit
(P41-ARC) (Actin-related protein 2/3 complex subunit 1B)
(SP:O88656) [Rattus norvegicus]
Length = 378
Score = 73.3 bits (172), Expect = 1e-13
Identities = 29/44 (65%), Positives = 37/44 (84%)
Frame = +3
Query: 507 DKFAVGSGARLISICYFEKENNWWVSKHIKKPIRSTVTSIDWHP 638
+KFAVGSGA+ + ICY+E+ENNWWVSK I+K S+VTS+ WHP
Sbjct: 112 NKFAVGSGAKTVCICYYEQENNWWVSKLIRKRHESSVTSVAWHP 155
Score = 58.4 bits (135), Expect = 4e-09
Identities = 37/106 (34%), Positives = 51/106 (48%), Gaps = 2/106 (1%)
Frame = +1
Query: 214 ITCHAWNKDRSQIA--SHQTIMKFTYTKGRK*LEANKQPCGTRP*SDGD*LGSQHQPYCY 387
ITCHAW+ D S +A + T + + + E G S+
Sbjct: 13 ITCHAWSPDLSMVALCPNNTEVHIYKSLSQDHWERLHVLQKHDQIVSGIDWSSKSNKIVT 72
Query: 388 VFVDRNAYVWTQGDDGKWATTLVLLRINRAATCVKWSPMETSLLSG 525
V DRN+YVW+ + +W TLV+LR+NRAA CV+WSP E G
Sbjct: 73 VSHDRNSYVWSL-EGAEWVPTLVILRLNRAALCVQWSPKENKFAVG 117
Score = 35.5 bits (78), Expect = 0.032
Identities = 17/40 (42%), Positives = 25/40 (62%), Gaps = 2/40 (5%)
Frame = +2
Query: 281 HIQKE--GNDWKQTNNLVEHDLRVMGIDWAPNTNRIVTCS 394
HI K + W++ + L +HD V GIDW+ +N+IVT S
Sbjct: 35 HIYKSLSQDHWERLHVLQKHDQIVSGIDWSSKSNKIVTVS 74
>At3g10790.1 68416.m01299 F-box family protein contains F-box domain
Pfam:PF00646
Length = 319
Score = 29.9 bits (64), Expect = 1.6
Identities = 14/60 (23%), Positives = 30/60 (50%)
Frame = +2
Query: 305 WKQTNNLVEHDLRVMGIDWAPNTNRIVTCSLTVMLTSGLKEMMGNGQLLLFFCVSTEQRL 484
+ ++ N+V + +V + R++ C++ LT G + + NG + F V+ +Q L
Sbjct: 191 YSRSGNIVSKEHKVFTLGCKQKKWRMIECTINHYLTPGTQGIFSNGVIYYFARVNDDQSL 250
>At4g34460.2 68417.m04898 guanine nucleotide-binding protein beta
subunit (GB1) / GTP-binding protein beta subunit (AGB1)
/ transducin contains 7 WD-40 repeats (PF00400);
identical to Guanine nucleotide-binding protein beta
subunit.SP:P49177 [Arabidopsis thaliana]; Weiss, CA et
al, PNAS 91:9954 (1994)
Length = 315
Score = 29.5 bits (63), Expect = 2.1
Identities = 11/20 (55%), Positives = 14/20 (70%)
Frame = +2
Query: 335 DLRVMGIDWAPNTNRIVTCS 394
DL+V +DW P NRIV+ S
Sbjct: 3 DLQVYSLDWTPERNRIVSAS 22
>At4g32551.1 68417.m04633 WD-40 repeat family protein (LEUNIG)
contains seven G-protein beta WD-40 repeats; beta
transducin-like protein, Podospora anserina, gb:L28125;
contains Pfam profiles PF04503: Single-stranded DNA
binding protein, SSDP; PF00400:WD domain, G-beta repeat;
identical to cDNA LEUNIG (LEUNIG) GI:11141604
Length = 931
Score = 27.9 bits (59), Expect = 6.5
Identities = 16/71 (22%), Positives = 29/71 (40%)
Frame = +3
Query: 426 R*WEMGNYSCSSAYQPSSDLREMVSHGDKFAVGSGARLISICYFEKENNWWVSKHIKKPI 605
R W + N SC+ Y+ S K+ S A L+++ E + +H +
Sbjct: 762 RYWSINNGSCTRVYKGGSTQIRFQPRVGKYLAASSANLVNVLDVETQ----AIRHSLQGH 817
Query: 606 RSTVTSIDWHP 638
+ + S+ W P
Sbjct: 818 ANPINSVCWDP 828
>At3g01780.1 68416.m00118 expressed protein est hit,
Length = 1176
Score = 27.9 bits (59), Expect = 6.5
Identities = 23/65 (35%), Positives = 32/65 (49%), Gaps = 5/65 (7%)
Frame = -1
Query: 417 PDVSITVNEHVTIRLVLGAQSIPITLRSCSTRLFVCFQSFPS----FCICELHYC-LVRS 253
PD +T V+I L A S+P + CS+ + CF S PS F I E C L R
Sbjct: 89 PDPDVTAAA-VSILAALPAFSLPKLISDCSSEIASCFDS-PSDNLRFSITETLGCILARD 146
Query: 252 NLTSI 238
+L ++
Sbjct: 147 DLVTL 151
>At2g01330.1 68415.m00050 transducin family protein / WD-40 repeat
family protein contains 10 WD-40 repeats (PF00400);
similar to 66kDa stress protein (SWISS-PROT: P90587)[
Physarum polycephalum (Slime mold)]
Length = 474
Score = 27.9 bits (59), Expect = 6.5
Identities = 9/27 (33%), Positives = 16/27 (59%)
Frame = +2
Query: 317 NNLVEHDLRVMGIDWAPNTNRIVTCSL 397
NN++ H R+ + W+PN + T S+
Sbjct: 393 NNMLFHTARINSLAWSPNNKMVATGSI 419
>At4g14220.1 68417.m02194 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 371
Score = 27.5 bits (58), Expect = 8.5
Identities = 16/39 (41%), Positives = 20/39 (51%)
Frame = -3
Query: 442 PISHHLLESRRKHYGQRTRNNTVGVGSPINPHHSKVVFH 326
P S LL + K +TRN + SPI+ HHS FH
Sbjct: 95 PASQELLAAVEKERLLKTRN--ISSSSPISIHHSHDDFH 131
>At3g01340.1 68416.m00051 protein transport protein SEC13 family
protein / WD-40 repeat family protein similar to Protein
transport protein SEC13 SP|Q04491 {Saccharomyces
cerevisiae} and SEC13 (SP:P53024) [Pichia pastoris]
Length = 302
Score = 27.5 bits (58), Expect = 8.5
Identities = 12/29 (41%), Positives = 16/29 (55%), Gaps = 2/29 (6%)
Frame = +2
Query: 290 KEGND--WKQTNNLVEHDLRVMGIDWAPN 370
KEGN W Q + +H + V I WAP+
Sbjct: 86 KEGNQNQWTQAHVFTDHKVSVNSIAWAPH 114
>At2g30050.1 68415.m03654 transducin family protein / WD-40 repeat
family protein similar to SEC13-related protein
(SP:P55735) [Homo sapiens]
Length = 302
Score = 27.5 bits (58), Expect = 8.5
Identities = 14/39 (35%), Positives = 18/39 (46%)
Frame = +3
Query: 546 ICYFEKENNWWVSKHIKKPIRSTVTSIDWHPITFCSSLA 662
I + E N W H+ +S+V SI W P SLA
Sbjct: 83 ILWKEGNQNQWTQDHVFTDHKSSVNSIAWAPHDIGLSLA 121
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,383,811
Number of Sequences: 28952
Number of extensions: 336083
Number of successful extensions: 849
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 794
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 846
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1412971776
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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