Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00626
         (734 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha Ef...   146   2e-36
SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha Ef...   146   2e-36
SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha Ef...   146   2e-36
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa...    46   7e-06
SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related prote...    32   0.098
SPAC631.01c |acp2||F-actin capping protein beta subunit |Schizos...    26   6.4  
SPBC9B6.04c |tuf1||mitochondrial translation elongation factor E...    25   8.5  

>SPAC23A1.10 |ef1a-b||translation elongation factor EF-1 alpha
           Ef1a-b |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 460

 Score =  146 bits (355), Expect = 2e-36
 Identities = 64/79 (81%), Positives = 72/79 (91%)
 Frame = +2

Query: 17  VPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLD 196
           +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLD
Sbjct: 301 LPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLD 360

Query: 197 CHTAHIACKFAEIKEKVDR 253
           CHTAHIACKFAE+ EK+DR
Sbjct: 361 CHTAHIACKFAELIEKIDR 379



 Score = 93.5 bits (222), Expect = 3e-20
 Identities = 42/59 (71%), Positives = 50/59 (84%)
 Frame = +1

Query: 256 TGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 432
           +GK  E +PK +KSGDA I  +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 381 SGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439


>SPCC794.09c |ef1a-a||translation elongation factor EF-1 alpha
           Ef1a-a |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 460

 Score =  146 bits (355), Expect = 2e-36
 Identities = 64/79 (81%), Positives = 72/79 (91%)
 Frame = +2

Query: 17  VPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLD 196
           +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLD
Sbjct: 301 LPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLD 360

Query: 197 CHTAHIACKFAEIKEKVDR 253
           CHTAHIACKFAE+ EK+DR
Sbjct: 361 CHTAHIACKFAELIEKIDR 379



 Score = 93.5 bits (222), Expect = 3e-20
 Identities = 42/59 (71%), Positives = 50/59 (84%)
 Frame = +1

Query: 256 TGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 432
           +GK  E +PK +KSGDA I  +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 381 SGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439


>SPBC839.15c |ef1a-c||translation elongation factor EF-1 alpha
           Ef1a-c |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 460

 Score =  146 bits (355), Expect = 2e-36
 Identities = 64/79 (81%), Positives = 72/79 (91%)
 Frame = +2

Query: 17  VPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLD 196
           +PGDNVGFNVKNVSVK++RRG V GDSKN+PP G A FTAQVI+LNHPGQIS GY+PVLD
Sbjct: 301 LPGDNVGFNVKNVSVKDIRRGNVCGDSKNDPPMGCASFTAQVIILNHPGQISAGYSPVLD 360

Query: 197 CHTAHIACKFAEIKEKVDR 253
           CHTAHIACKFAE+ EK+DR
Sbjct: 361 CHTAHIACKFAELIEKIDR 379



 Score = 93.5 bits (222), Expect = 3e-20
 Identities = 42/59 (71%), Positives = 50/59 (84%)
 Frame = +1

Query: 256 TGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVGVIKAV 432
           +GK  E +PK +KSGDA I  +VPSKP+CVE+F ++ PLGRFAVRDMRQTVAVGVIKAV
Sbjct: 381 SGKKIEESPKFVKSGDACIAKMVPSKPMCVEAFTDYAPLGRFAVRDMRQTVAVGVIKAV 439


>SPCC584.04 |sup35|erf3|translation release factor eRF3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 662

 Score = 45.6 bits (103), Expect = 7e-06
 Identities = 25/80 (31%), Positives = 42/80 (52%)
 Frame = +2

Query: 14  AVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVL 193
           ++ GD V   V+     +++ GYV   +KN P      F AQ+ +L  P  ++ GY+ V+
Sbjct: 524 SICGDQVRLRVRGDD-SDVQTGYVLTSTKN-PVHATTRFIAQIAILELPSILTTGYSCVM 581

Query: 194 DCHTAHIACKFAEIKEKVDR 253
             HTA     FA++  K+D+
Sbjct: 582 HIHTAVEEVSFAKLLHKLDK 601



 Score = 42.7 bits (96), Expect = 5e-05
 Identities = 21/61 (34%), Positives = 34/61 (55%), Gaps = 1/61 (1%)
 Frame = +1

Query: 256 TGKSTEVNPKSIKSGDAAIVNLVPSKPLCVESFQEFPPLGRFAVRDMRQTVAVG-VIKAV 432
           T + ++  P     G   I  L    P+C+E F+++  +GRF +RD   TVAVG V+K +
Sbjct: 602 TNRKSKKPPMFATKGMKIIAELETQTPVCMERFEDYQYMGRFTLRDQGTTVAVGKVVKIL 661

Query: 433 N 435
           +
Sbjct: 662 D 662


>SPBC25B2.01 ||SPBC2G5.08|elongation factor 1 alpha related
           protein|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 592

 Score = 31.9 bits (69), Expect = 0.098
 Identities = 15/31 (48%), Positives = 20/31 (64%)
 Frame = +1

Query: 334 PLCVESFQEFPPLGRFAVRDMRQTVAVGVIK 426
           PLC+   +E P LGRF +R    TVA G++K
Sbjct: 561 PLCLA--EECPALGRFILRRSGDTVAAGIVK 589


>SPAC631.01c |acp2||F-actin capping protein beta subunit
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 268

 Score = 25.8 bits (54), Expect = 6.4
 Identities = 17/53 (32%), Positives = 27/53 (50%)
 Frame = -1

Query: 311 MAASPDLMDFGLTSVDLPVDGQLFL*FLQICRQCGQCGNPILVCNRLRFDQDG 153
           ++ +PDL D  L+SVD P+              C + GN  L+C+   F++DG
Sbjct: 27  LSVAPDLADVLLSSVDQPLK----------VNTCSESGNQYLLCD---FNRDG 66


>SPBC9B6.04c |tuf1||mitochondrial translation elongation factor
           EF-Tu Tuf1 |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 439

 Score = 25.4 bits (53), Expect = 8.5
 Identities = 9/24 (37%), Positives = 17/24 (70%)
 Frame = +2

Query: 14  AVPGDNVGFNVKNVSVKELRRGYV 85
           AV GDN G  ++++  ++L+RG +
Sbjct: 311 AVAGDNCGLLLRSIKREQLKRGMI 334


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,973,389
Number of Sequences: 5004
Number of extensions: 60082
Number of successful extensions: 165
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 155
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 165
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 347244562
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)