BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= wdS00620 (631 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g06830.1 68418.m00772 expressed protein contains Pfam profile... 64 6e-11 At1g67230.1 68414.m07652 expressed protein 31 0.63 At4g24280.1 68417.m03486 heat shock protein 70, putative / HSP70... 30 1.5 At1g47550.1 68414.m05278 expressed protein 29 1.9 At2g39380.1 68415.m04833 exocyst subunit EXO70 family protein co... 29 2.5 At5g10360.1 68418.m01202 40S ribosomal protein S6 (RPS6B) 28 4.4 At4g31700.1 68417.m04500 40S ribosomal protein S6 (RPS6A) riboso... 28 4.4 At2g27280.1 68415.m03278 hypothetical protein 28 4.4 At1g34140.1 68414.m04235 polyadenylate-binding protein, putative... 28 4.4 At1g20400.1 68414.m02544 myosin heavy chain-related 28 4.4 At3g56300.1 68416.m06258 tRNA synthetase class I (C) family prot... 27 7.8 At1g22300.3 68414.m02790 14-3-3 protein GF14 epsilon (GRF10) ide... 27 7.8 At1g22300.2 68414.m02789 14-3-3 protein GF14 epsilon (GRF10) ide... 27 7.8 At1g22300.1 68414.m02788 14-3-3 protein GF14 epsilon (GRF10) ide... 27 7.8 >At5g06830.1 68418.m00772 expressed protein contains Pfam profile: PF05600 protein of unknown function (DUF773) Length = 549 Score = 64.5 bits (150), Expect = 6e-11 Identities = 26/74 (35%), Positives = 47/74 (63%) Frame = +3 Query: 249 QEISYEIPGLKRQIAKEEQAQADFEKKHADYLKNEASSKSEFLALCKQLGIQGEKIKREL 428 Q ++YEIP LK+Q+ K +Q + ++K AD ++ A S +++ C++LG+QG ++REL Sbjct: 123 QNVNYEIPYLKKQVQKVQQQMTELDRKEADIKRSVALSATKYEDACRELGLQGNNVRREL 182 Query: 429 VAKLQELPEIYDQI 470 + LP + +I Sbjct: 183 LETASSLPSTFSKI 196 Score = 47.6 bits (108), Expect = 7e-06 Identities = 22/65 (33%), Positives = 39/65 (60%) Frame = +1 Query: 64 LSGSYINYFHCLKIIEILKETEADTKNLFGRYGSQXMKDWQDVERNYEKENLYLAXAAQM 243 L I Y K+ EIL +T +++N+FGR S W+ + R +EK+++YL AAQ+ Sbjct: 62 LDPEVIGYLEVKKVYEILLKTTPESRNIFGRL-SGASGVWEAIVRAFEKDHIYLGEAAQI 120 Query: 244 LVRKL 258 +++ + Sbjct: 121 IIQNV 125 >At1g67230.1 68414.m07652 expressed protein Length = 1132 Score = 31.1 bits (67), Expect = 0.63 Identities = 22/68 (32%), Positives = 34/68 (50%), Gaps = 2/68 (2%) Frame = +3 Query: 249 QEISYEIPGLKRQIAKEEQAQADFEKKHADYLKNEASSKSEFLALCKQLGIQGEKIK--R 422 +EI + L +++ E QAQ K D L+ +SE+L L +L Q EK + + Sbjct: 445 KEIILNLKALVEKVSGENQAQLSEINKEKDELRVTEEERSEYLRLQTELKEQIEKCRSQQ 504 Query: 423 ELVAKLQE 446 EL+ K E Sbjct: 505 ELLQKEAE 512 >At4g24280.1 68417.m03486 heat shock protein 70, putative / HSP70, putative strong similarity to heat shock protein 70 [Arabidopsis thaliana] GI:6746592; similar to heat shock 70 protein - Spinacia oleracea,PID:g2654208 Length = 718 Score = 29.9 bits (64), Expect = 1.5 Identities = 24/60 (40%), Positives = 33/60 (55%), Gaps = 1/60 (1%) Frame = +3 Query: 297 EEQAQADFEKKHADYLKNEASSKSEFLALCKQLGIQGEKIKRELVAKLQ-ELPEIYDQIG 473 E A+ D EK+ A KN+A S KQL GEKI E+ K++ +L E+ D+IG Sbjct: 591 ERFAKDDKEKRDAIDTKNQADSV--VYQTEKQLKELGEKIPGEVKEKVEAKLQELKDKIG 648 >At1g47550.1 68414.m05278 expressed protein Length = 887 Score = 29.5 bits (63), Expect = 1.9 Identities = 22/91 (24%), Positives = 45/91 (49%) Frame = +3 Query: 276 LKRQIAKEEQAQADFEKKHADYLKNEASSKSEFLALCKQLGIQGEKIKRELVAKLQELPE 455 +K + A+ E+ +A F ++ +++L+N +S +F+ K Q ++KR A L+ Sbjct: 371 VKEKRAELEKLKATFVRRASEFLRNYFASLVDFMVSDKSYFSQRGQLKRPDHADLRYKCR 430 Query: 456 IYDQIGKSLKPLQPGIEFIMHSQSISSVRIL 548 Y ++ + LK L + SS+ +L Sbjct: 431 TYARLLQHLKGLDKNCLGPLRKAYCSSLNLL 461 >At2g39380.1 68415.m04833 exocyst subunit EXO70 family protein contains Pfam domain PF03081: Exo70 exocyst complex subunit; Length = 637 Score = 29.1 bits (62), Expect = 2.5 Identities = 25/81 (30%), Positives = 43/81 (53%), Gaps = 5/81 (6%) Frame = +3 Query: 321 EKKHADYLKNEASSKSE--FLALCKQLGIQGEKIKRELVAKLQELPE-IYD--QIGKSLK 485 EK D++ + +S+ E F + + GI K ELVA+ + PE I+ + ++ Sbjct: 261 EKLLCDHVFSASSTIRESCFYEIVNEAGINLFKFP-ELVAEKKPSPERIFRLMDLYAAIS 319 Query: 486 PLQPGIEFIMHSQSISSVRIL 548 L+P IE I H S+++V+ L Sbjct: 320 DLRPDIELIFHFDSVAAVKTL 340 >At5g10360.1 68418.m01202 40S ribosomal protein S6 (RPS6B) Length = 249 Score = 28.3 bits (60), Expect = 4.4 Identities = 14/34 (41%), Positives = 20/34 (58%) Frame = +3 Query: 279 KRQIAKEEQAQADFEKKHADYLKNEASSKSEFLA 380 K++IAK AD++K A LK + +SE LA Sbjct: 200 KKRIAKANSDAADYQKLLASRLKEQRDRRSESLA 233 >At4g31700.1 68417.m04500 40S ribosomal protein S6 (RPS6A) ribosomal protein S6, Arabidopsis thaliana, PID:g2662469 Length = 250 Score = 28.3 bits (60), Expect = 4.4 Identities = 14/34 (41%), Positives = 20/34 (58%) Frame = +3 Query: 279 KRQIAKEEQAQADFEKKHADYLKNEASSKSEFLA 380 K++IAK AD++K A LK + +SE LA Sbjct: 200 KKKIAKANSDAADYQKLLASRLKEQRDRRSESLA 233 >At2g27280.1 68415.m03278 hypothetical protein Length = 427 Score = 28.3 bits (60), Expect = 4.4 Identities = 22/77 (28%), Positives = 40/77 (51%) Frame = +3 Query: 252 EISYEIPGLKRQIAKEEQAQADFEKKHADYLKNEASSKSEFLALCKQLGIQGEKIKRELV 431 EI YE L ++ AK+E +D EK K + + ++L ++ ++ + +R+ V Sbjct: 255 EIVYERK-LAKERAKDEHLYSDKEKFVTGPFKRKLEEQKKWL---EEERLRELREERDDV 310 Query: 432 AKLQELPEIYDQIGKSL 482 K +L E Y IGK++ Sbjct: 311 TKKNDLSEFYINIGKNV 327 >At1g34140.1 68414.m04235 polyadenylate-binding protein, putative / PABP, putative non-consensus splice donor TA at exon 1; similar to polyadenylate-binding protein (poly(A)-binding protein) from [Triticum aestivum] GI:1737492, [Nicotiana tabacum] GI:7673355, {Arabidopsis thaliana} SP|P42731; contains InterPro entry IPR000504: RNA-binding region RNP-1 (RNA recognition motif) (RRM) Length = 407 Score = 28.3 bits (60), Expect = 4.4 Identities = 17/49 (34%), Positives = 24/49 (48%), Gaps = 4/49 (8%) Frame = +1 Query: 124 TEADTKNLFGRYG----SQXMKDWQDVERNYEKENLYLAXAAQMLVRKL 258 T+AD K LFG +G + MKD + R + N A AA + K+ Sbjct: 131 TDADLKRLFGEFGEITSAVVMKDGEGKSRRFGFVNFEKAEAAVTAIEKM 179 >At1g20400.1 68414.m02544 myosin heavy chain-related Length = 944 Score = 28.3 bits (60), Expect = 4.4 Identities = 23/99 (23%), Positives = 46/99 (46%), Gaps = 3/99 (3%) Frame = +3 Query: 276 LKRQIAKEEQAQADFEKKHADYLKNEASSKSEFLALCKQLGIQGEKIKR---ELVAKLQE 446 L+ +++ E++ + KK K A KSE L Q + GE+ R +L + ++ Sbjct: 708 LRGELSAEKKKNDELLKKLESASKEAAHLKSEVATLAYQRTVMGEERDRCTLDLEKEREK 767 Query: 447 LPEIYDQIGKSLKPLQPGIEFIMHSQSISSVRILQKLYQ 563 E+ D++ K L+ E +Q+ ++ + L+Q Sbjct: 768 TVELEDRLKSEKKRLRSRREKYAENQTSKALIHVADLFQ 806 >At3g56300.1 68416.m06258 tRNA synthetase class I (C) family protein similar to cysteinyl-tRNA synthetase [Methanococcus maripaludis] GI:6599476; contains Pfam profile PF01406: tRNA synthetases class I (C) Length = 489 Score = 27.5 bits (58), Expect = 7.8 Identities = 16/43 (37%), Positives = 22/43 (51%), Gaps = 1/43 (2%) Frame = -2 Query: 504 LYLAGVVLVISLFGHISQAAPAVS-PLILS*SSHLGYQVVYTK 379 +Y+ G+ + HI A AVS L+ HLGYQV Y + Sbjct: 34 IYVCGITAYD--YSHIGHARAAVSFDLLYRYLRHLGYQVTYVR 74 >At1g22300.3 68414.m02790 14-3-3 protein GF14 epsilon (GRF10) identical to 14-3-3 protein GF14 epsilon GI:5802798, SP:P48347 from [Arabidopsis thaliana] Length = 251 Score = 27.5 bits (58), Expect = 7.8 Identities = 14/48 (29%), Positives = 30/48 (62%), Gaps = 1/48 (2%) Frame = +3 Query: 399 IQGEKIKRELVAKLQELPEIYDQIGKSLKPL-QPGIEFIMHSQSISSV 539 ++ E+ K+ +AKL E E YD++ +++K + Q +E + +++ SV Sbjct: 1 MENEREKQVYLAKLSEQTERYDEMVEAMKKVAQLDVELTVEERNLVSV 48 >At1g22300.2 68414.m02789 14-3-3 protein GF14 epsilon (GRF10) identical to 14-3-3 protein GF14 epsilon GI:5802798, SP:P48347 from [Arabidopsis thaliana] Length = 254 Score = 27.5 bits (58), Expect = 7.8 Identities = 14/48 (29%), Positives = 30/48 (62%), Gaps = 1/48 (2%) Frame = +3 Query: 399 IQGEKIKRELVAKLQELPEIYDQIGKSLKPL-QPGIEFIMHSQSISSV 539 ++ E+ K+ +AKL E E YD++ +++K + Q +E + +++ SV Sbjct: 1 MENEREKQVYLAKLSEQTERYDEMVEAMKKVAQLDVELTVEERNLVSV 48 >At1g22300.1 68414.m02788 14-3-3 protein GF14 epsilon (GRF10) identical to 14-3-3 protein GF14 epsilon GI:5802798, SP:P48347 from [Arabidopsis thaliana] Length = 254 Score = 27.5 bits (58), Expect = 7.8 Identities = 14/48 (29%), Positives = 30/48 (62%), Gaps = 1/48 (2%) Frame = +3 Query: 399 IQGEKIKRELVAKLQELPEIYDQIGKSLKPL-QPGIEFIMHSQSISSV 539 ++ E+ K+ +AKL E E YD++ +++K + Q +E + +++ SV Sbjct: 1 MENEREKQVYLAKLSEQTERYDEMVEAMKKVAQLDVELTVEERNLVSV 48 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,290,430 Number of Sequences: 28952 Number of extensions: 234877 Number of successful extensions: 757 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 738 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 756 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1285411824 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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