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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= wdS00620
         (631 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g06830.1 68418.m00772 expressed protein contains Pfam profile...    64   6e-11
At1g67230.1 68414.m07652 expressed protein                             31   0.63 
At4g24280.1 68417.m03486 heat shock protein 70, putative / HSP70...    30   1.5  
At1g47550.1 68414.m05278 expressed protein                             29   1.9  
At2g39380.1 68415.m04833 exocyst subunit EXO70 family protein co...    29   2.5  
At5g10360.1 68418.m01202 40S ribosomal protein S6 (RPS6B)              28   4.4  
At4g31700.1 68417.m04500 40S ribosomal protein S6 (RPS6A) riboso...    28   4.4  
At2g27280.1 68415.m03278 hypothetical protein                          28   4.4  
At1g34140.1 68414.m04235 polyadenylate-binding protein, putative...    28   4.4  
At1g20400.1 68414.m02544 myosin heavy chain-related                    28   4.4  
At3g56300.1 68416.m06258 tRNA synthetase class I (C) family prot...    27   7.8  
At1g22300.3 68414.m02790 14-3-3 protein GF14 epsilon (GRF10) ide...    27   7.8  
At1g22300.2 68414.m02789 14-3-3 protein GF14 epsilon (GRF10) ide...    27   7.8  
At1g22300.1 68414.m02788 14-3-3 protein GF14 epsilon (GRF10) ide...    27   7.8  

>At5g06830.1 68418.m00772 expressed protein contains Pfam profile:
           PF05600 protein of unknown function (DUF773)
          Length = 549

 Score = 64.5 bits (150), Expect = 6e-11
 Identities = 26/74 (35%), Positives = 47/74 (63%)
 Frame = +3

Query: 249 QEISYEIPGLKRQIAKEEQAQADFEKKHADYLKNEASSKSEFLALCKQLGIQGEKIKREL 428
           Q ++YEIP LK+Q+ K +Q   + ++K AD  ++ A S +++   C++LG+QG  ++REL
Sbjct: 123 QNVNYEIPYLKKQVQKVQQQMTELDRKEADIKRSVALSATKYEDACRELGLQGNNVRREL 182

Query: 429 VAKLQELPEIYDQI 470
           +     LP  + +I
Sbjct: 183 LETASSLPSTFSKI 196



 Score = 47.6 bits (108), Expect = 7e-06
 Identities = 22/65 (33%), Positives = 39/65 (60%)
 Frame = +1

Query: 64  LSGSYINYFHCLKIIEILKETEADTKNLFGRYGSQXMKDWQDVERNYEKENLYLAXAAQM 243
           L    I Y    K+ EIL +T  +++N+FGR  S     W+ + R +EK+++YL  AAQ+
Sbjct: 62  LDPEVIGYLEVKKVYEILLKTTPESRNIFGRL-SGASGVWEAIVRAFEKDHIYLGEAAQI 120

Query: 244 LVRKL 258
           +++ +
Sbjct: 121 IIQNV 125


>At1g67230.1 68414.m07652 expressed protein
          Length = 1132

 Score = 31.1 bits (67), Expect = 0.63
 Identities = 22/68 (32%), Positives = 34/68 (50%), Gaps = 2/68 (2%)
 Frame = +3

Query: 249 QEISYEIPGLKRQIAKEEQAQADFEKKHADYLKNEASSKSEFLALCKQLGIQGEKIK--R 422
           +EI   +  L  +++ E QAQ     K  D L+     +SE+L L  +L  Q EK +  +
Sbjct: 445 KEIILNLKALVEKVSGENQAQLSEINKEKDELRVTEEERSEYLRLQTELKEQIEKCRSQQ 504

Query: 423 ELVAKLQE 446
           EL+ K  E
Sbjct: 505 ELLQKEAE 512


>At4g24280.1 68417.m03486 heat shock protein 70, putative / HSP70,
           putative strong similarity to heat shock protein 70
           [Arabidopsis thaliana] GI:6746592; similar to heat shock
           70 protein - Spinacia oleracea,PID:g2654208
          Length = 718

 Score = 29.9 bits (64), Expect = 1.5
 Identities = 24/60 (40%), Positives = 33/60 (55%), Gaps = 1/60 (1%)
 Frame = +3

Query: 297 EEQAQADFEKKHADYLKNEASSKSEFLALCKQLGIQGEKIKRELVAKLQ-ELPEIYDQIG 473
           E  A+ D EK+ A   KN+A S        KQL   GEKI  E+  K++ +L E+ D+IG
Sbjct: 591 ERFAKDDKEKRDAIDTKNQADSV--VYQTEKQLKELGEKIPGEVKEKVEAKLQELKDKIG 648


>At1g47550.1 68414.m05278 expressed protein
          Length = 887

 Score = 29.5 bits (63), Expect = 1.9
 Identities = 22/91 (24%), Positives = 45/91 (49%)
 Frame = +3

Query: 276 LKRQIAKEEQAQADFEKKHADYLKNEASSKSEFLALCKQLGIQGEKIKRELVAKLQELPE 455
           +K + A+ E+ +A F ++ +++L+N  +S  +F+   K    Q  ++KR   A L+    
Sbjct: 371 VKEKRAELEKLKATFVRRASEFLRNYFASLVDFMVSDKSYFSQRGQLKRPDHADLRYKCR 430

Query: 456 IYDQIGKSLKPLQPGIEFIMHSQSISSVRIL 548
            Y ++ + LK L       +     SS+ +L
Sbjct: 431 TYARLLQHLKGLDKNCLGPLRKAYCSSLNLL 461


>At2g39380.1 68415.m04833 exocyst subunit EXO70 family protein
           contains Pfam domain PF03081: Exo70 exocyst complex
           subunit;
          Length = 637

 Score = 29.1 bits (62), Expect = 2.5
 Identities = 25/81 (30%), Positives = 43/81 (53%), Gaps = 5/81 (6%)
 Frame = +3

Query: 321 EKKHADYLKNEASSKSE--FLALCKQLGIQGEKIKRELVAKLQELPE-IYD--QIGKSLK 485
           EK   D++ + +S+  E  F  +  + GI   K   ELVA+ +  PE I+    +  ++ 
Sbjct: 261 EKLLCDHVFSASSTIRESCFYEIVNEAGINLFKFP-ELVAEKKPSPERIFRLMDLYAAIS 319

Query: 486 PLQPGIEFIMHSQSISSVRIL 548
            L+P IE I H  S+++V+ L
Sbjct: 320 DLRPDIELIFHFDSVAAVKTL 340


>At5g10360.1 68418.m01202 40S ribosomal protein S6 (RPS6B)
          Length = 249

 Score = 28.3 bits (60), Expect = 4.4
 Identities = 14/34 (41%), Positives = 20/34 (58%)
 Frame = +3

Query: 279 KRQIAKEEQAQADFEKKHADYLKNEASSKSEFLA 380
           K++IAK     AD++K  A  LK +   +SE LA
Sbjct: 200 KKRIAKANSDAADYQKLLASRLKEQRDRRSESLA 233


>At4g31700.1 68417.m04500 40S ribosomal protein S6 (RPS6A) ribosomal
           protein S6, Arabidopsis thaliana, PID:g2662469
          Length = 250

 Score = 28.3 bits (60), Expect = 4.4
 Identities = 14/34 (41%), Positives = 20/34 (58%)
 Frame = +3

Query: 279 KRQIAKEEQAQADFEKKHADYLKNEASSKSEFLA 380
           K++IAK     AD++K  A  LK +   +SE LA
Sbjct: 200 KKKIAKANSDAADYQKLLASRLKEQRDRRSESLA 233


>At2g27280.1 68415.m03278 hypothetical protein 
          Length = 427

 Score = 28.3 bits (60), Expect = 4.4
 Identities = 22/77 (28%), Positives = 40/77 (51%)
 Frame = +3

Query: 252 EISYEIPGLKRQIAKEEQAQADFEKKHADYLKNEASSKSEFLALCKQLGIQGEKIKRELV 431
           EI YE   L ++ AK+E   +D EK      K +   + ++L   ++  ++  + +R+ V
Sbjct: 255 EIVYERK-LAKERAKDEHLYSDKEKFVTGPFKRKLEEQKKWL---EEERLRELREERDDV 310

Query: 432 AKLQELPEIYDQIGKSL 482
            K  +L E Y  IGK++
Sbjct: 311 TKKNDLSEFYINIGKNV 327


>At1g34140.1 68414.m04235 polyadenylate-binding protein, putative /
           PABP, putative non-consensus splice donor TA at exon 1;
           similar to polyadenylate-binding protein
           (poly(A)-binding protein) from [Triticum aestivum]
           GI:1737492, [Nicotiana tabacum] GI:7673355, {Arabidopsis
           thaliana} SP|P42731; contains InterPro entry IPR000504:
           RNA-binding region RNP-1 (RNA recognition motif) (RRM)
          Length = 407

 Score = 28.3 bits (60), Expect = 4.4
 Identities = 17/49 (34%), Positives = 24/49 (48%), Gaps = 4/49 (8%)
 Frame = +1

Query: 124 TEADTKNLFGRYG----SQXMKDWQDVERNYEKENLYLAXAAQMLVRKL 258
           T+AD K LFG +G    +  MKD +   R +   N   A AA   + K+
Sbjct: 131 TDADLKRLFGEFGEITSAVVMKDGEGKSRRFGFVNFEKAEAAVTAIEKM 179


>At1g20400.1 68414.m02544 myosin heavy chain-related
          Length = 944

 Score = 28.3 bits (60), Expect = 4.4
 Identities = 23/99 (23%), Positives = 46/99 (46%), Gaps = 3/99 (3%)
 Frame = +3

Query: 276  LKRQIAKEEQAQADFEKKHADYLKNEASSKSEFLALCKQLGIQGEKIKR---ELVAKLQE 446
            L+ +++ E++   +  KK     K  A  KSE   L  Q  + GE+  R   +L  + ++
Sbjct: 708  LRGELSAEKKKNDELLKKLESASKEAAHLKSEVATLAYQRTVMGEERDRCTLDLEKEREK 767

Query: 447  LPEIYDQIGKSLKPLQPGIEFIMHSQSISSVRILQKLYQ 563
              E+ D++    K L+   E    +Q+  ++  +  L+Q
Sbjct: 768  TVELEDRLKSEKKRLRSRREKYAENQTSKALIHVADLFQ 806


>At3g56300.1 68416.m06258 tRNA synthetase class I (C) family protein
           similar to cysteinyl-tRNA synthetase [Methanococcus
           maripaludis] GI:6599476; contains Pfam profile PF01406:
           tRNA synthetases class I (C)
          Length = 489

 Score = 27.5 bits (58), Expect = 7.8
 Identities = 16/43 (37%), Positives = 22/43 (51%), Gaps = 1/43 (2%)
 Frame = -2

Query: 504 LYLAGVVLVISLFGHISQAAPAVS-PLILS*SSHLGYQVVYTK 379
           +Y+ G+      + HI  A  AVS  L+     HLGYQV Y +
Sbjct: 34  IYVCGITAYD--YSHIGHARAAVSFDLLYRYLRHLGYQVTYVR 74


>At1g22300.3 68414.m02790 14-3-3 protein GF14 epsilon (GRF10)
           identical to 14-3-3 protein GF14 epsilon GI:5802798,
           SP:P48347 from [Arabidopsis thaliana]
          Length = 251

 Score = 27.5 bits (58), Expect = 7.8
 Identities = 14/48 (29%), Positives = 30/48 (62%), Gaps = 1/48 (2%)
 Frame = +3

Query: 399 IQGEKIKRELVAKLQELPEIYDQIGKSLKPL-QPGIEFIMHSQSISSV 539
           ++ E+ K+  +AKL E  E YD++ +++K + Q  +E  +  +++ SV
Sbjct: 1   MENEREKQVYLAKLSEQTERYDEMVEAMKKVAQLDVELTVEERNLVSV 48


>At1g22300.2 68414.m02789 14-3-3 protein GF14 epsilon (GRF10)
           identical to 14-3-3 protein GF14 epsilon GI:5802798,
           SP:P48347 from [Arabidopsis thaliana]
          Length = 254

 Score = 27.5 bits (58), Expect = 7.8
 Identities = 14/48 (29%), Positives = 30/48 (62%), Gaps = 1/48 (2%)
 Frame = +3

Query: 399 IQGEKIKRELVAKLQELPEIYDQIGKSLKPL-QPGIEFIMHSQSISSV 539
           ++ E+ K+  +AKL E  E YD++ +++K + Q  +E  +  +++ SV
Sbjct: 1   MENEREKQVYLAKLSEQTERYDEMVEAMKKVAQLDVELTVEERNLVSV 48


>At1g22300.1 68414.m02788 14-3-3 protein GF14 epsilon (GRF10)
           identical to 14-3-3 protein GF14 epsilon GI:5802798,
           SP:P48347 from [Arabidopsis thaliana]
          Length = 254

 Score = 27.5 bits (58), Expect = 7.8
 Identities = 14/48 (29%), Positives = 30/48 (62%), Gaps = 1/48 (2%)
 Frame = +3

Query: 399 IQGEKIKRELVAKLQELPEIYDQIGKSLKPL-QPGIEFIMHSQSISSV 539
           ++ E+ K+  +AKL E  E YD++ +++K + Q  +E  +  +++ SV
Sbjct: 1   MENEREKQVYLAKLSEQTERYDEMVEAMKKVAQLDVELTVEERNLVSV 48


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,290,430
Number of Sequences: 28952
Number of extensions: 234877
Number of successful extensions: 757
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 738
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 756
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1285411824
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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