Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= wdS00599
(739 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 281 5e-76
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 281 5e-76
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 281 5e-76
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 281 5e-76
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 168 4e-42
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 161 4e-40
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 95 4e-20
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 87 9e-18
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 53 2e-07
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 53 2e-07
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 52 5e-07
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 48 5e-06
At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 47 1e-05
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 44 1e-04
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 40 0.002
At4g18330.2 68417.m02719 eukaryotic translation initiation facto... 38 0.007
At4g18330.1 68417.m02718 eukaryotic translation initiation facto... 38 0.007
At5g13650.2 68418.m01585 elongation factor family protein contai... 37 0.012
At5g13650.1 68418.m01584 elongation factor family protein contai... 37 0.012
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 37 0.016
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 37 0.016
At1g04170.1 68414.m00407 eukaryotic translation initiation facto... 36 0.021
At2g18720.1 68415.m02180 eukaryotic translation initiation facto... 36 0.037
At4g11160.1 68417.m01808 translation initiation factor IF-2, mit... 33 0.26
At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein s... 31 0.80
At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329... 31 1.1
At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329... 31 1.1
At1g76825.1 68414.m08940 eukaryotic translation initiation facto... 30 1.8
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 30 1.8
At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 30 1.8
At1g21160.1 68414.m02646 eukaryotic translation initiation facto... 29 2.4
At5g38140.1 68418.m04596 histone-like transcription factor (CBF/... 28 5.6
At1g76530.1 68414.m08906 auxin efflux carrier family protein con... 28 7.4
At1g17220.1 68414.m02098 translation initiation factor IF-2, chl... 28 7.4
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 281 bits (688), Expect = 5e-76
Identities = 134/155 (86%), Positives = 141/155 (90%)
Frame = +3
Query: 45 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 224
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 225 DKLKAERERRITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 404
DKLKAERER ITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 405 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKM 509
G FEAGISK+GQTREHALLAFTLGVKQ+I NKM
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKM 155
Score = 56.4 bits (130), Expect = 2e-08
Identities = 23/37 (62%), Positives = 30/37 (81%)
Frame = +2
Query: 509 DSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVP 619
D+T P YS+ R++EI KEVSSY+KK+GYNP + FVP
Sbjct: 156 DATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVP 192
Score = 36.7 bits (81), Expect = 0.016
Identities = 15/29 (51%), Positives = 19/29 (65%)
Frame = +1
Query: 595 PSCCRFRAISGWHGDNMLEPSTKMPWFKG 681
P F ISG+ GDNM+E ST + W+KG
Sbjct: 185 PDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 281 bits (688), Expect = 5e-76
Identities = 134/155 (86%), Positives = 141/155 (90%)
Frame = +3
Query: 45 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 224
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 225 DKLKAERERRITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 404
DKLKAERER ITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 405 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKM 509
G FEAGISK+GQTREHALLAFTLGVKQ+I NKM
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKM 155
Score = 56.4 bits (130), Expect = 2e-08
Identities = 23/37 (62%), Positives = 30/37 (81%)
Frame = +2
Query: 509 DSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVP 619
D+T P YS+ R++EI KEVSSY+KK+GYNP + FVP
Sbjct: 156 DATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVP 192
Score = 36.7 bits (81), Expect = 0.016
Identities = 15/29 (51%), Positives = 19/29 (65%)
Frame = +1
Query: 595 PSCCRFRAISGWHGDNMLEPSTKMPWFKG 681
P F ISG+ GDNM+E ST + W+KG
Sbjct: 185 PDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 281 bits (688), Expect = 5e-76
Identities = 134/155 (86%), Positives = 141/155 (90%)
Frame = +3
Query: 45 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 224
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 225 DKLKAERERRITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 404
DKLKAERER ITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 405 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKM 509
G FEAGISK+GQTREHALLAFTLGVKQ+I NKM
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKM 155
Score = 56.4 bits (130), Expect = 2e-08
Identities = 23/37 (62%), Positives = 30/37 (81%)
Frame = +2
Query: 509 DSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVP 619
D+T P YS+ R++EI KEVSSY+KK+GYNP + FVP
Sbjct: 156 DATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVP 192
Score = 36.7 bits (81), Expect = 0.016
Identities = 15/29 (51%), Positives = 19/29 (65%)
Frame = +1
Query: 595 PSCCRFRAISGWHGDNMLEPSTKMPWFKG 681
P F ISG+ GDNM+E ST + W+KG
Sbjct: 185 PDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 281 bits (688), Expect = 5e-76
Identities = 134/155 (86%), Positives = 141/155 (90%)
Frame = +3
Query: 45 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 224
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 225 DKLKAERERRITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 404
DKLKAERER ITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + T
Sbjct: 61 DKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTT 120
Query: 405 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKM 509
G FEAGISK+GQTREHALLAFTLGVKQ+I NKM
Sbjct: 121 GGFEAGISKDGQTREHALLAFTLGVKQMICCCNKM 155
Score = 56.4 bits (130), Expect = 2e-08
Identities = 23/37 (62%), Positives = 30/37 (81%)
Frame = +2
Query: 509 DSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVP 619
D+T P YS+ R++EI KEVSSY+KK+GYNP + FVP
Sbjct: 156 DATTPKYSKARYDEIIKEVSSYLKKVGYNPDKIPFVP 192
Score = 36.7 bits (81), Expect = 0.016
Identities = 15/29 (51%), Positives = 19/29 (65%)
Frame = +1
Query: 595 PSCCRFRAISGWHGDNMLEPSTKMPWFKG 681
P F ISG+ GDNM+E ST + W+KG
Sbjct: 185 PDKIPFVPISGFEGDNMIERSTNLDWYKG 213
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 168 bits (408), Expect = 4e-42
Identities = 74/151 (49%), Positives = 108/151 (71%), Gaps = 1/151 (0%)
Frame = +3
Query: 60 THINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKA 239
+ +N+ ++GHVDSGKST +G L++ G I ++ + K+EKEA+ GKGSF YAW LD+
Sbjct: 238 SQLNLAIVGHVDSGKSTLSGRLLHLLGRISQKQMHKYEKEAKLQGKGSFAYAWALDESAE 297
Query: 240 ERERRITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEA 419
ERER IT+ +A+ F + +++V ++D+PGH+DF+ NMI G +QAD A+L++ A G FEA
Sbjct: 298 ERERGITMTVAVAYFNSKRHHVVLLDSPGHKDFVPNMIAGATQADAAILVIDASVGAFEA 357
Query: 420 GISK-NGQTREHALLAFTLGVKQLIVGVNKM 509
G GQTREHA + GV+Q+IV +NKM
Sbjct: 358 GFDNLKGQTREHARVLRGFGVEQVIVAINKM 388
Score = 28.7 bits (61), Expect = 4.3
Identities = 16/71 (22%), Positives = 37/71 (52%)
Frame = +2
Query: 527 YSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPFLDGTETTCWSLQPKCLGSRDAVERKEGK 706
YS+ RF+ IK+ V S+++ + +++ ++P +++ + L + + R
Sbjct: 393 YSKERFDLIKQHVGSFLQSCRFKDSSLTWIPLS--------AMENQNLVAAPSDNRLSSW 444
Query: 707 ADGKCLIEALD 739
G CL++A+D
Sbjct: 445 YQGPCLLDAVD 455
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 161 bits (391), Expect = 4e-40
Identities = 74/152 (48%), Positives = 106/152 (69%)
Frame = +3
Query: 54 EKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL 233
+K H+N+V IGHVD+GKST G +++ G +D R I+K+EKEA++ + S+ A+++D
Sbjct: 98 KKRHLNVVFIGHVDAGKSTIGGQILFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMDTN 157
Query: 234 KAERERRITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEF 413
+ ER + T+++ FET TI+DAPGH+ ++ NMI+G SQAD VL+++A GEF
Sbjct: 158 EEERLKGKTVEVGRAHFETESTRFTILDAPGHKSYVPNMISGASQADIGVLVISARKGEF 217
Query: 414 EAGISKNGQTREHALLAFTLGVKQLIVGVNKM 509
E G + GQTREH LA TLGV +LIV VNKM
Sbjct: 218 ETGYERGGQTREHVQLAKTLGVSKLIVVVNKM 249
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 95.1 bits (226), Expect = 4e-20
Identities = 56/155 (36%), Positives = 81/155 (52%)
Frame = +3
Query: 42 KMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWV 221
K ++K H+NI IGHVD GK+T T L I +K+++
Sbjct: 72 KFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDE--------------- 116
Query: 222 LDKLKAERERRITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG 401
+D ER R ITI+ A ++ET + +D PGH D++KNMITG +Q D A+L+V+
Sbjct: 117 IDAAPEERARGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 176
Query: 402 TGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNK 506
G QT+EH LLA +GV ++V +NK
Sbjct: 177 DGPMP-------QTKEHILLAKQVGVPDMVVFLNK 204
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 87.4 bits (207), Expect = 9e-18
Identities = 55/153 (35%), Positives = 79/153 (51%)
Frame = +3
Query: 51 KEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDK 230
+ K H+N+ IGHVD GK+T T + K E GK +DK
Sbjct: 63 RNKPHVNVGTIGHVDHGKTTLTAAIT---------------KVLAEEGKAKAIAFDEIDK 107
Query: 231 LKAERERRITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGE 410
E++R ITI A ++ET+K + +D PGH D++KNMITG +Q D +L+V+ G
Sbjct: 108 APEEKKRGITIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGP 167
Query: 411 FEAGISKNGQTREHALLAFTLGVKQLIVGVNKM 509
QT+EH LLA +GV L+ +NK+
Sbjct: 168 MP-------QTKEHILLARQVGVPSLVCFLNKV 193
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 53.2 bits (122), Expect = 2e-07
Identities = 40/128 (31%), Positives = 60/128 (46%)
Frame = +3
Query: 69 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 248
NI + H+DSGK+T T +++ G I E E ++ G G+ +D + ERE
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRIH----EIHEVRGRD-GVGA-----KMDSMDLERE 116
Query: 249 RRITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 428
+ ITI A Y V IID PGH DF + D A+L++ + G I+
Sbjct: 117 KGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSIT 176
Query: 429 KNGQTREH 452
+ Q R +
Sbjct: 177 VDRQMRRY 184
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 53.2 bits (122), Expect = 2e-07
Identities = 40/128 (31%), Positives = 60/128 (46%)
Frame = +3
Query: 69 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 248
NI + H+DSGK+T T +++ G I E E ++ G G+ +D + ERE
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRIH----EIHEVRGRD-GVGA-----KMDSMDLERE 116
Query: 249 RRITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGIS 428
+ ITI A Y V IID PGH DF + D A+L++ + G I+
Sbjct: 117 KGITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSIT 176
Query: 429 KNGQTREH 452
+ Q R +
Sbjct: 177 VDRQMRRY 184
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 51.6 bits (118), Expect = 5e-07
Identities = 36/113 (31%), Positives = 53/113 (46%)
Frame = +3
Query: 69 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 248
NI ++ HVD GK+T HLI GG + GK F +D L E+
Sbjct: 11 NICILAHVDHGKTTLADHLIASSGG--------GVLHPRLAGKLRF-----MDYLDEEQR 57
Query: 249 RRITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 407
R IT+ + + Y + +ID+PGH DF + T +D A+++V A G
Sbjct: 58 RAITMKSSSISLKYKDYSLNLIDSPGHMDFCSEVSTAARLSDGALVLVDAVEG 110
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 48.4 bits (110), Expect = 5e-06
Identities = 35/117 (29%), Positives = 55/117 (47%)
Frame = +3
Query: 57 KTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLK 236
K + NI ++ H+D+GK+TTT ++Y G K E+ +G+ W ++
Sbjct: 94 KDYRNIGIMAHIDAGKTTTTERILYYTGRNYK---------IGEVHEGTATMDW----ME 140
Query: 237 AERERRITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 407
E+ER ITI A K+ + IID PGH DF + D A+ + + G
Sbjct: 141 QEQERGITITSAATTTFWDKHRINIIDTPGHVDFTLEVERALRVLDGAICLFDSVAG 197
>At5g08650.1 68418.m01029 GTP-binding protein LepA, putative
Length = 681
Score = 46.8 bits (106), Expect = 1e-05
Identities = 35/117 (29%), Positives = 53/117 (45%), Gaps = 4/117 (3%)
Frame = +3
Query: 69 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 248
N +I H+D GKST L+ G + R + K F LD + ERE
Sbjct: 88 NFSIIAHIDHGKSTLADKLLQVTGTVQNRDM-----------KEQF-----LDNMDLERE 131
Query: 249 RRITIDIAL----WKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 407
R ITI + + +E + + + +ID PGH DF + + + A+L+V A G
Sbjct: 132 RGITIKLQAARMRYVYEDTPFCLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQG 188
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 43.6 bits (98), Expect = 1e-04
Identities = 42/132 (31%), Positives = 54/132 (40%), Gaps = 7/132 (5%)
Frame = +3
Query: 33 D*PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKY 212
D K EK N +I H+D GKST L+ G I K G G +Y
Sbjct: 57 DLTKFPSEKIR-NFSIIAHIDHGKSTLADRLMELTGTIKK-------------GHGQPQY 102
Query: 213 AWVLDKLKAERERRITIDIALWKFET-------SKYYVTIIDAPGHRDFIKNMITGTSQA 371
LDKL+ ER + A +E S Y + +ID PGH DF + S
Sbjct: 103 ---LDKLQRERGITVKAQTATMFYENKVEDQEASGYLLNLIDTPGHVDFSYEVSRSLSAC 159
Query: 372 DCAVLIVAAGTG 407
A+L+V A G
Sbjct: 160 QGALLVVDAAQG 171
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 39.5 bits (88), Expect = 0.002
Identities = 31/112 (27%), Positives = 51/112 (45%), Gaps = 4/112 (3%)
Frame = +3
Query: 69 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRT---IEKFEKEAQEMGKG-SFKYAWVLDKLK 236
N+ VI HVD GKST T L+ G I + + + A E +G + K + +
Sbjct: 21 NMSVIAHVDHGKSTLTDSLVAAAGIIAQEVAGDVRMTDTRADEAERGITIKSTGISLYYE 80
Query: 237 AERERRITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 392
E + A + ++Y + +ID+PGH DF + D A+++V
Sbjct: 81 MTDESLKSFTGAR---DGNEYLINLIDSPGHVDFSSEVTAALRITDGALVVV 129
>At4g18330.2 68417.m02719 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 471
Score = 37.9 bits (84), Expect = 0.007
Identities = 45/175 (25%), Positives = 77/175 (44%), Gaps = 14/175 (8%)
Frame = +3
Query: 39 PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK-RTIEKFEKEAQ-EMGKGSFK- 209
P++ + INI IGHV GKST ++ G+ R + E+ ++G + K
Sbjct: 31 PEVISRQATINIGTIGHVAHGKST----IVKAISGVQTVRFKNELERNITIKLGYANAKI 86
Query: 210 YAWVLDKLK---------AERERRITIDIALWKF--ETSKYYVTIIDAPGHRDFIKNMIT 356
Y DK + +E T D+ ++ T + +V+ +D PGH + M+
Sbjct: 87 YKCEDDKCPRPMCYKSYGSGKEDNPTCDVPGFENCKMTLRRHVSCVDCPGHDILMATMLN 146
Query: 357 GTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMIPLN 521
G + D A+L++AA QT EH + +K +I+ NK+ +N
Sbjct: 147 GAAIVDGALLLIAANES------CPQPQTAEHLASVDMMRLKHIIILQNKIDLIN 195
>At4g18330.1 68417.m02718 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 284
Score = 37.9 bits (84), Expect = 0.007
Identities = 45/175 (25%), Positives = 77/175 (44%), Gaps = 14/175 (8%)
Frame = +3
Query: 39 PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK-RTIEKFEKEAQ-EMGKGSFK- 209
P++ + INI IGHV GKST ++ G+ R + E+ ++G + K
Sbjct: 31 PEVISRQATINIGTIGHVAHGKST----IVKAISGVQTVRFKNELERNITIKLGYANAKI 86
Query: 210 YAWVLDKLK---------AERERRITIDIALWKF--ETSKYYVTIIDAPGHRDFIKNMIT 356
Y DK + +E T D+ ++ T + +V+ +D PGH + M+
Sbjct: 87 YKCEDDKCPRPMCYKSYGSGKEDNPTCDVPGFENCKMTLRRHVSCVDCPGHDILMATMLN 146
Query: 357 GTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMIPLN 521
G + D A+L++AA QT EH + +K +I+ NK+ +N
Sbjct: 147 GAAIVDGALLLIAANES------CPQPQTAEHLASVDMMRLKHIIILQNKIDLIN 195
>At5g13650.2 68418.m01585 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 676
Score = 37.1 bits (82), Expect = 0.012
Identities = 32/113 (28%), Positives = 48/113 (42%)
Frame = +3
Query: 69 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 248
NI ++ HVD GK+T ++ + K ++ Q M + ++D ERE
Sbjct: 85 NIAIVAHVDHGKTTLVDSMLRQA---------KVFRDNQVMQER------IMDSNDLERE 129
Query: 249 RRITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 407
R ITI V IID PGH DF + + D +L+V + G
Sbjct: 130 RGITILSKNTSITYKNTKVNIIDTPGHSDFGGEVERVLNMVDGVLLVVDSVEG 182
>At5g13650.1 68418.m01584 elongation factor family protein contains
Pfam profiles: PF00009 elongation factor Tu GTP binding
domain,PF00679 elongation factor G C-terminus, PF03144
elongation factor Tu domain 2
Length = 675
Score = 37.1 bits (82), Expect = 0.012
Identities = 32/113 (28%), Positives = 48/113 (42%)
Frame = +3
Query: 69 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 248
NI ++ HVD GK+T ++ + K ++ Q M + ++D ERE
Sbjct: 84 NIAIVAHVDHGKTTLVDSMLRQA---------KVFRDNQVMQER------IMDSNDLERE 128
Query: 249 RRITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 407
R ITI V IID PGH DF + + D +L+V + G
Sbjct: 129 RGITILSKNTSITYKNTKVNIIDTPGHSDFGGEVERVLNMVDGVLLVVDSVEG 181
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 36.7 bits (81), Expect = 0.016
Identities = 35/118 (29%), Positives = 49/118 (41%), Gaps = 5/118 (4%)
Frame = +3
Query: 69 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 248
N+ ++GH+ GK+ L+ + + K EK KY D E+E
Sbjct: 140 NVALVGHLQHGKTVFMDMLVEQTHHMSTFNA-KNEKH--------MKYT---DTRVDEQE 187
Query: 249 RRITI-----DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 407
R I+I + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 188 RNISIKAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 36.7 bits (81), Expect = 0.016
Identities = 35/118 (29%), Positives = 49/118 (41%), Gaps = 5/118 (4%)
Frame = +3
Query: 69 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERE 248
N+ ++GH+ GK+ L+ + + K EK KY D E+E
Sbjct: 140 NVALVGHLQHGKTVFMDMLVEQTHHMSTFNA-KNEKH--------MKYT---DTRVDEQE 187
Query: 249 RRITI-----DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 407
R I+I + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 188 RNISIKAVPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At1g04170.1 68414.m00407 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to gb|U37354 from S. pombe. ESTs
gb|T41979, gb|N37284 and gb|N37529 come from this gene
Length = 465
Score = 36.3 bits (80), Expect = 0.021
Identities = 49/191 (25%), Positives = 83/191 (43%), Gaps = 15/191 (7%)
Frame = +3
Query: 39 PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK-RTIEKFEKEAQ-EMGKGSFKY 212
P++ + INI IGHV GKST ++ G+ R + E+ ++G + K
Sbjct: 25 PEVISRQATINIGTIGHVAHGKST----VVKAISGVQTVRFKNELERNITIKLGYANAKI 80
Query: 213 AWVLDKLKAERE---------RRITIDIALWKFETSKY----YVTIIDAPGHRDFIKNMI 353
D+ K R + T + + FE SK +V+ +D PGH + M+
Sbjct: 81 YKCEDE-KCPRPMCYKAYGSGKEDTPNCDVPGFENSKMKLLRHVSFVDCPGHDILMATML 139
Query: 354 TGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMIPLNHHTV 533
G + D A+L++AA QT EH + +K +I+ NK I L V
Sbjct: 140 NGAAIMDGALLLIAANE------TCPQPQTSEHLAAVEIMQLKHIIILQNK-IDLIQENV 192
Query: 534 SPDLRKSRRKY 566
+ + ++ +K+
Sbjct: 193 AINQHEAIQKF 203
>At2g18720.1 68415.m02180 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative
Length = 465
Score = 35.5 bits (78), Expect = 0.037
Identities = 41/165 (24%), Positives = 70/165 (42%), Gaps = 8/165 (4%)
Frame = +3
Query: 39 PKMGKEKTHINIVVIGHVDSGKST----TTG-HLIYKCGGIDKR-TIEKFEKEAQEMGKG 200
P++ + INI IGHV GKST +G H + +++ TI+ A+
Sbjct: 25 PEVISRQATINIGTIGHVAHGKSTIVKAVSGVHTVKFKSELERNITIKLGYANAKIYKCE 84
Query: 201 SFKYAWVLDKLKAERERRITIDIALWKFETSKY--YVTIIDAPGHRDFIKNMITGTSQAD 374
+ +E + D++ + K +V+ +D PGH + M+ G + D
Sbjct: 85 KCPRPVAYKSFGSGKEDNPSCDVSGHEKYKMKLLRHVSFVDCPGHDILMATMLNGAAIMD 144
Query: 375 CAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKM 509
A+LI+AA QT EH + +K +I+ NK+
Sbjct: 145 GALLIIAANE------TCPQPQTAEHLASVDMMHLKDIIIIQNKI 183
>At4g11160.1 68417.m01808 translation initiation factor IF-2,
mitochondrial, putative similar to SP|P46198|IF2M_BOVIN
Translation initiation factor IF-2, mitochondrial
precursor (IF-2Mt) (IF-2(Mt)) {Bos taurus}
Length = 743
Score = 32.7 bits (71), Expect = 0.26
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +3
Query: 303 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 407
+T +D PGH F + G + D VL+VAA G
Sbjct: 270 ITFLDTPGHAAFSEMRARGAAVTDIVVLVVAADDG 304
>At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein
similar to 1,4-alpha-glucan branching enzyme [Solanum
tuberosum] GI:1621012, 1,4-alpha-glucan branching enzyme
(EC 2.4.1.18) from [Homo sapiens] SP|Q04446, {Solanum
tuberosum} SP|P30924; contains Pfam profiles: PF00128
Alpha amylase catalytic domain, PF02922 Isoamylase
N-terminal domain
Length = 777
Score = 31.1 bits (67), Expect = 0.80
Identities = 25/78 (32%), Positives = 36/78 (46%), Gaps = 8/78 (10%)
Frame = +2
Query: 530 SEPR---FEEIKKEVSSYIKKIGYNPAAVAFVP-----FLDGTETTCWSLQPKCLGSRDA 685
SEP+ FEE K+V ++K+ GYN + VP F G T + G+ D
Sbjct: 257 SEPKVSTFEEFTKKVLPHVKRAGYNAIQLIGVPEHKDYFTVGYRVTNFFAASSRYGTPDD 316
Query: 686 VERKEGKADGKCLIEALD 739
+R +A G L+ LD
Sbjct: 317 FKRLVDEAHGLGLLVFLD 334
>At5g44785.2 68418.m05490 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 442
Score = 30.7 bits (66), Expect = 1.1
Identities = 16/42 (38%), Positives = 23/42 (54%)
Frame = +2
Query: 506 NDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPFLDG 631
N +TE + P+ E K E+S++I IG+ V F P DG
Sbjct: 61 NVATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCSDG 102
>At5g44785.1 68418.m05489 expressed protein contains Pfam PF05329:
Protein of unknown function (DUF731)
Length = 440
Score = 30.7 bits (66), Expect = 1.1
Identities = 16/42 (38%), Positives = 23/42 (54%)
Frame = +2
Query: 506 NDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPFLDG 631
N +TE + P+ E K E+S++I IG+ V F P DG
Sbjct: 61 NVATEKESTPPKKIEYKPEISNWINLIGFVEQPVQFGPCSDG 102
>At1g76825.1 68414.m08940 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profile PF00009: Elongation
factor Tu GTP binding domain
Length = 630
Score = 29.9 bits (64), Expect = 1.8
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +3
Query: 309 IIDAPGHRDFIKNMITGTSQADCAVLIV 392
+ID PGH F G+S D A+L+V
Sbjct: 113 VIDTPGHESFTNLRSRGSSLCDLAILVV 140
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 29.9 bits (64), Expect = 1.8
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +3
Query: 309 IIDAPGHRDFIKNMITGTSQADCAVLIV 392
+ID PGH F G+S D A+L+V
Sbjct: 772 VIDTPGHESFTNLRSRGSSLCDLAILVV 799
>At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1201
Score = 29.9 bits (64), Expect = 1.8
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +3
Query: 309 IIDAPGHRDFIKNMITGTSQADCAVLIV 392
+ID PGH F G+S D A+L+V
Sbjct: 705 VIDTPGHESFTNLRSRGSSLCDLAILVV 732
>At1g21160.1 68414.m02646 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1088
Score = 29.5 bits (63), Expect = 2.4
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = +3
Query: 303 VTIIDAPGHRDFIKNMITGTSQADCAVLIV 392
+ +ID PGH F G++ D A+L+V
Sbjct: 558 ILVIDTPGHESFTNLRSRGSNLCDLAILVV 587
>At5g38140.1 68418.m04596 histone-like transcription factor
(CBF/NF-Y) family protein similar to CCAAT-binding
transcription factor subunit AAB-1 (GI:2583171)
[Neurospora crassa]; contains a CBF/NF-Y subunit
signature (PDOC00578) presernt in members of
histone-like transcription factor family; contains Pfam
PF00808 : Histone-like transcription factor (CBF/NF-Y)
and archaeal histone
Length = 195
Score = 28.3 bits (60), Expect = 5.6
Identities = 16/55 (29%), Positives = 29/55 (52%)
Frame = +3
Query: 492 VGVNKMIPLNHHTVSPDLRKSRRKYPHTSRRLATTQLLSLSCHFWMARRQHVGAF 656
V + + P +H+T+ P L + R +P TS+ + L + FW +R+ +G F
Sbjct: 9 VRMEPVAPRSHNTM-PMLDQFRSNHPETSKIEGVSSLDTALKVFWNNQREQLGNF 62
>At1g76530.1 68414.m08906 auxin efflux carrier family protein
contains auxin efflux carrier domain, Pfam:PF03547
Length = 415
Score = 27.9 bits (59), Expect = 7.4
Identities = 15/57 (26%), Positives = 28/57 (49%), Gaps = 4/57 (7%)
Frame = +2
Query: 458 ARFHPRCQTAHRRSKQNDSTEPPYSEPRFEEIKKEVSSYIKKIG----YNPAAVAFV 616
+ + C+ SK+ + E + R+EE+K+ V S KK+ + PA +A +
Sbjct: 201 SNYDDSCKVQLISSKEEEKEEDNHQVGRWEEVKQRVVSLSKKVNLGSIFAPATIAAI 257
>At1g17220.1 68414.m02098 translation initiation factor IF-2,
chloroplast, putative similar to SP|P57997|IF2C_PHAVU
Translation initiation factor IF-2, chloroplast
precursor (PvIF2cp) {Phaseolus vulgaris}
Length = 1026
Score = 27.9 bits (59), Expect = 7.4
Identities = 12/32 (37%), Positives = 16/32 (50%)
Frame = +3
Query: 312 IDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 407
+D PGH F G D A+++VAA G
Sbjct: 557 LDTPGHEAFGAMRARGARVTDIAIIVVAADDG 588
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,292,980
Number of Sequences: 28952
Number of extensions: 348131
Number of successful extensions: 1130
Number of sequences better than 10.0: 34
Number of HSP's better than 10.0 without gapping: 1070
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1121
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1624036432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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